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| 1 | Quantitative analysis of vascular endothelial growth factor, microvascular density and their clinicopathologic features in human hepatocellular carcinoma显示文摘BACKGROUND: Angiogenesis is known to be essential to the survival, growth, invasion, and metastasis of tumor cells. Vascular endothelial growth factor (VEGF) are an important angiogenic factor regulating tumor angiogenesis, but its significance and tumor pathologic features are un- clear in hepatocellular carcinoma (HCC). In the present study, we analyzed expression of tissue VEGF, alteration of microvascular density (MVD) in microvessel angiogenesis, development and metastasis of HCC, and level of serum VEGF in differential diagnosis of benign and malignant liv- er diseases. METHODS: Tumor specimens were prospectively collected from HCC patients undergoing resection. Total RNAs were extracted and the expression levels were detected from different parts of HCC tissues. The cellular distributions of VEGF and MVD of liver tumors and their paracancerous and distal cancerous tissues were investigated by streptavi- din peroxidase (S-P) immunohistochemistry, respectively. The VEGF levels of circulating blood and hepatoma tissues were measured by enzyme-linked immunosorbent assay. RESULTS: The incidence of VEGF expression was 63.9% in HCCs (23/36 cases), 78.3% in non-encapsulated HCCs (18/23), and 90.9% in HCCs with extrahepatic metastasis (10/11), respectively. The VEGF expression was tightly correlated with MVD (P <0.01). The MVD in HCC with metastasis, low differentiation or non-encapsulation was significantly higher than that in HCC with intact capsule, high differentiation, or no metastasis. No significant diffe- rence was found between VEGF, MVD, tumor size, and hepatitis virus infection. The level of total RNA in HCC tis- sues was significantly lower but the VEGF level significantly higher than those in paracancerous or distal cancerous ones (P<0.01). The abnormal expression levels of VEGF in sera of HCC patients were directly correlated with the me- tastasis and recurrence of tumors. CONCLUSION: The high expression of VEGF and abnor- mality of tissue MVD are useful predictors for vascular inva- sion and metastasis of liver tumors. | Deng-Fu Yao, Xin-Hua Wu, Yong Zhu, Gong-Sheng Shi, Zhi-Zhen Dong, Deng-Bing Yao, Wei Wu, Li-Wei Qiu and Xian-Yong Meng Nantong, China Research Center of Clinical Molecular Biology , Department of Pathology and Department of Gastroenterology , Affiliated Hos- pital of Nantong University Department of Diagnostics , and Institute of Neurosciences , Nantong University Nantong 226001, China | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，2： | 81 |
| 2 | Oncofetal antigen glypican-3 as a promising early diagnostic marker for hepatocellular carcinoma显示文摘BACKGROUND:Hepatocellular carcinoma (HCC) is characterized by a multi-cause,multi-stage and multi-focus process of tumor progression.Its prognosis is poor and early diagnosis is of utmost importance.This study was undertaken to investigate the dynamic expression of oncofetal antigen glypican-3 (GPC-3) and GPC-3 mRNA in hepatocarcinogenesis and to explore their early diagnostic value for HCC.METHODS:A hepatoma model was induced in male Sprague-Dawley rats with 0.05% 2-fluorenylacetamide and confirmed by hematoxylin and eosin staining and gamma-glutamyltransferase (GGT) expression.Total RNA was purified and transcribed into cDNA by reverse transcription.Fragments of the GPC-3 gene were amplified by nested RT-PCR,and confirmed by sequencing.GPC-3 was analyzed by immunohistochemistry,Western blotting or ELISA.RESULTS:Positive GPC-3 expression showed as brown granule-like staining localized in the cytoplasm.Histological examination of hepatocytes revealed three morphological stages of granule-like degeneration,atypical hyperplasia (precancerous),and cancer formation,with a progressive increase of liver total RNA and GGT expression.The incidence of liver GPC-3 mRNA and GPC-3,and serum GPC-3 was 100%,100% and 77.8% in the HCC group,100%,100%,and 66.7% in the precancerous group,83.3%,83.3%,and 38.9% in the degeneration group,and no expression in the liver or blood of the control group,respectively.There was a positive correlation between liver GPC-3 mRNA and total RNA level (r=0.475,P<0.05) or liver GPC-3 (r=1.0,P<0.001) or serum GPC-3 (r= 0.994,P<0.001).CONCLUSION:Abnormal oncofetal antigen GPC-3 and GPC-3 mRNA expression in hepatocarcinogenesis may be promising molecular markers for early diagnosis of HCC. | Research Center of Clinical Medicine,Affiliated Hospital of Nantong University,Nantong 226001,China (Yao M,Yao DF,Qiu LW,Wu W,Sai WL,Yang JL and Zhang HJ) Department of Oncology,Yancheng First People’s Hospital,Yancheng 224001,China (Bian YZ) Department of Oncology,Second Affiliated Hospital,Nanjing Medical University,Nanjing 210011,China (Zhang CG) | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，3： | 51 |
| 3 | Quantitative analysis of hepatic hypoxia-inducible factor-1α and its abnormal gene expression during the formation of hepatocellular carcinoma显示文摘BACKGROUND:Hepatic hypoxia-inducible factor-1(HIF-1) is activated in the progression of hepatocellular carcinoma (HCC).This study aimed to investigate the dynamic alterations of HIF-1αand its gene expression so as to explore the relationship between HIF-1αexpression and hepatocarcinogenesis at the early stage of HCC. METHODS:A hepatoma model was made with 2-fluorenyl- acetamide(2-FAA)in male Sprague-Dawley rats.Morphological changes of rat hepatocytes were assessed pathologically (HE staining).The dynamic expression of hepatic and circulating HIF-1αwas quantitatively analyzed by ELISA. The gene fragments of hepatic HIF-1αmRNA were amplified by RT-PCR and confirmed by sequencing.The cellular distribution of hepatic HIF-1αexpression was confirmed by immunohistochemistry. RESULTS:Histological examination confirmed granulelike degeneration to atypical hyperplasia and HCC development in rat hepatocytes and progressive increases in the levels of hepatic and circulating HIF-1αand its gene expression during the course.The levels of HIF-1α expression in the liver and blood of rats with hepatoma were significantly higher than those in normal ratsand those with degeneration.Immunohistochemical analysis confirmed the positive expression and hepatocyte distribution of HIF-1αin the development of rat hepatoma. A positive relationship was found between HIF-1α expression in the liver and blood(P<0.01). CONCLUSIONS:The above observations support the hypothesis that the overexpression of HIF-1αand its gene are closely associated with the malignant transformation of hepatocytes and play an important role at the stage of hepatocarcinogenesis. | Deng-Fu Yao,Hua Jiang,Min Yao,Yue-Ming Li,Wen-Jing Gu,Yu-Cheng Shen, Li-Wei Qiu,Wei Wu,Xin-Hua Wu and Wen-Li Sai Research Center of Clinical Molecular Biology,Department of Laboratory Science,and Department of Oncology,Affiliated Hospital of Nantong University,Nantong 226001, China Third Department of Medicine,Jiaonan People’s Hospital,Jiaonan 266400,China | 2009 | Hepatobiliary & Pancreatic Diseases International2009，8，4： | 28 |
| 4 | Clinical impact of plasma TGF-β1 and circulating TGF-β1 mRNA in diagnosis of hepatocellular carcinoma显示文摘BACKGROUND:Transforming growth factor-β(TGF-β) plays an important role in the regulation of cell growth and differentiation,angiogenesis,extracellular matrix formation,immunosuppression and cancer development. In this study,we investigated the levels of TGF-β1 and TGF-β1 mRNA expression,their relationship with HBV replication,and their diagnostic value for hepatocellular carcinoma(HCC). METHODS:Total RNAs were extracted from HCC samples and matched non-tumor tissues,and from peripheral blood mononuclear cells in HCC patients.TGF-β1 mRNA was amplified by RT-PCR and confirmed by DNA sequencing. The distribution of TGF-β1 expression was assessed by immunohistochemistry.The clinical characteristics were analyzed between TGF-β1 and HBV replication.The diagnostic value of circulating TGF-β1 and TGF-β1 mRNA levels were investigated in HCC patients. RESULTS:The incidence of hepatic TGF-β1 expression was 83.3%in HCC samples,43.3%in the surrounding tissues, 94.7%in the HBV DNA-positive group,and 63.6%in the HBV DNA-negative group.Liver TGF-β1 expression was associated with the degree of HCC differentiation and the status of HBV replication,but not with the size or number of tumors.Circulating TGF-β1 level and incidence of TGF-β1 mRNA were significantly higher in the HCC groupthan in any group of patients with benign liver disease, with a higher sensitivity of 89.5%and a specificity of 94.0% for HCC diagnosis when circulating TGF-β1 levels were >1.2μg/L.No significant correlation was found between TGF-β1 expression and AFP level or tumor size.Combining TGF-β1 level and serum AFP raised the detection rate to 97.4%. CONCLUSIONS:Abnormal expression of hepatic TGF-β1 is associated with the degree of HCC differentiation and HBV replication.Both circulating TGF-β1 and TGF-β1 mRNA can be used as sensitive biomarkers for the diagnosis and prognosis of HBV-induced HCC. | Zhi-Zhen Dong,Deng-Fu Yao,Min Yao,Li-Wei Qiu,Lei Zong,Wei Wu, Xin-Hua Wu,Deng-Bing Yao and Xian-Yong Meng Department of Diagnostics,Research Center of Clinical Molecular Biology,Department of Laboratory Science,and Department of GastroenterologyAffiliated Hospital and Institute of Neurosciences,Nantong University,Nantong 226001,China | 2008 | Hepatobiliary & Pancreatic Diseases International2008，7，3： | 27 |
| 5 | Combined serum hepatoma-specific alpha-fetoprotein and circulating alpha-fetoprotein-mRNA in diagnosis of hepatocellular carcinoma显示文摘BACKGROUND: Hepatocellular carcinoma (HCC) is one of the most common malignant tumors worldwide, its prognosis is poor, and early detection is of utmost importance. Although alpha-fetoprotein (AFP) is a useful marker for detecting and monitoring HCC development, the false-negative or false-positive rates with AFP alone may be as high as 30%-40% for patients with small HCCs. To enhance the specificity and accuracy of AFP measurements for HCC, we analyzed AFP expression states in livers, detected the hepatoma-specific AFP (HS-AFP) fraction and AFP-mRNA from peripheral blood mononuclear cells, and explored their clinical implications for HCC diagnosis. METHODS: AFP expression and hepatocyte distributions in liver specimens were investigated by an immunohistoche- mical assay. Total RNAs were extracted from circulating blood, synthesized to cDNA through random primers and reverse transcriptase, and fragments of the AFP gene were amplified by a nested-PCR assay. The HS-AFP fraction was separated by lectin-affinity chromatography and its level was detected by radioimmunoassay. RESULTS: The incidence of AFP was 73.3% in HCC tissues and its expression in HCCs with moderate or low differentiation was significantly stronger than that of HCCs with high differentiation (P<0.05). The incidence of AFP gene fragments was 100% in HCCs, and 60% in paracancerous tissues (P<0.01). In the HCC and liver cirrhosis groups, the incidence of HS-AFP was 91.7% and 18% (P<0.01), and of AFP-mRNA was 56.7% and 16% (P<0.01), respectively, and neither was found in controls.HS-AFP or AFP-mRNA was not significantly related to size or number of HCC, but to its differentiation, metastasis, and relapse (P<0.05). CONCLUSIONS: Different AFP expression is present in different parts of HCC tissues. HS-AFP and AFP-mRNA fragments improve sensitivity and specificity, and both are useful markers to diagnose HCC or monitor metastasis and relapse. | Wei Wu, Deng-Fu Yao, Yong-Mei Yuan, Ji-Wei Fan, Xiu-Feng Lu, Xiao-Hua Li, Li-Wei Qiu, Lei Zong and Xin-Hua Wu Research Center of Clinical Molecular Biology, Affiliated Hospital of Nantong University, Nantong 226001, China | 2006 | Hepatobiliary & Pancreatic Diseases International2006，5，4： | 26 |
| 6 | Abnormal expression of hepatoma- derived γ-glutamyltransferase subtyping and its early alteration for carcinogenesis of hepatocytes显示文摘BACKGROUND: Although the hepatoma-specific band of gamma-glutamyltransferase ( GGT ) is a highly sensitive marker in diagnosis of hepatocellular carcinoma, the kine- tic expression and the early alterations of GGT in the deve- lopment of hepatoma remain unclear. In this study, we in- vestigated the expression and the alterations of GGT multi- ple molecular forms in hepatotumorigenesis. METHODS: The expression of GGT in a chemically in- duced hepatocarcinogenesis model was examined by giving 0. 05% of 2-fluoenylacetamide in diet for 12 weeks. The ex- pression levels of total RNA and GGT, and the changes of liver pathology, GGT multiple molecular forms and sugar- chain heterogeneity were investigated at the different stages of rat hepatoma development. RESULTS: Pathological examination and biochemical ana- lysis found that liver GGT was over-expressed and secreted into blood during canceration. Serum total GGT and liver GGT specific activities (IU/g) including soluble and mem- brane-combined GGT were significantly higher (P <0.05) in experimental groups than those in control group, respec- tively. A highly positive correlation was found between to- tal GGT activities and total RNA levels (r =0.90, P <0.05) of the liver. Both were higher six weeks later than before. Con A-non-reactive-GGT was increased consistantly dur- ing the development of rat hepatoma. GGT multiple mo- lecular forms in the liver and sera of experimental rats showed that fetal liver-type GGT bands were associated with the development of hepatoma. CONCLUSIONS: Fetal liver-type GGT in sera and the liver of rats is closely related to hepatotumorigenesis. It can be used as a sensitive enzymatic marker for the early diagnosis of liver cancer. | Deng-Fu Yao, Zhi-Zhen Dong, Deng-Bing Yao, Xin-Hua Wu, Wei Wu, Li-Wei Qiu, Hong-Mei Wang and Xian-Yong Meng Nantong, China Research Center of Clinical Molecular Biology, Affilia- ted Hospital of Nantong University, Nantong 226001 , China Insti- tute of Neuroscicnces, Nantong University, Nantong 226001 , China | 2004 | Hepatobiliary & Pancreatic Diseases International2004，3，4： | 17 |
| 7 | Abnormal expression of HSP gp96 associated with HBV replication in human hepatocellular carcinoma显示文摘BACKGROUND: Heat shock protein (HSP) gp96 is a member of the HSP90 family and presumably overexpresses as a result of stimulation by mutated or abnormal proteins. Its abnormal expression correlates with carcinogenesis, progression and prognosis of hepatocellular carcinoma (HCC). In this study, we investigated the pathological characteristics of liver gp96 expression and its relationship with hepatitis B virus (HBV) replication in HCC patients. METHODS: Tumor specimens were prospectively collected from 30 HCC patients undergoing liver resection. Total RNAs were extracted from HCC or their noncancerous tissues. The distribution of gp96 expression in hepatocytes was investigated by streptavidin peroxidase (S-P) immunohistochemistry and tissue HBV-DNA was detected by the in situ molecular hybridization technique. The association of gp96 expression with HBV replication, and the histopathological characteristics of HCC were analyzed. RESULTS: The gp96 was strongly expressed in HCC (73.3%, 22 of 30) and weakly (46.7%, 14 of 30) in non-cancerous tissues. The gp96 expression in HCC tissues was correlated with degree of tumor differentiation and tumor size, but not with tumor number (P>0.05). Immunohistochemical analysis showed that 17 of 19 HCC patients with HBVDNA -positive were strongly expressed for gp96, whereas only 5 of 11 patients with HBV-DNA-negative were positive for gp96. A significant difference was found between the two groups (89.5% vs. 45.5%, P<0.05). CONCLUSIONS: The abnormal expressions of HSP gp96 in HCC tissues are associated with HBV replication. This finding indicates that HBV infection plays an important role in the development of HCC. | Deng-Fu Yao,Xin-Hua Wu,Xiao-Qin Su,Min Yao, Wei Wu, Li-Wei Qiu, Li Zou and Xian-Yong Meng The Institute of Clinical Medicine, Nantong University, and Department of Gastroenterology,Affiliated Hospital of Nantong University, Nantong 226001, China and Nanjing Medical University, Nanjing 210009, China | 2006 | Hepatobiliary & Pancreatic Diseases International2006，5，3： | 14 |
| 8 | GJB2 mutation spectrum in deaf population in a typical southeastern area of China显示文摘Mutations in GJB2 gene are the most frequently found mutations in patients with nonsyndromic hearing impairment. However, the spectrum and prevalence of mutations in this gene vary among different ethnic groups. In China, 30,000 infants are born with congenital hearing impairment annually. In order to provide appropriate genetic testing and counseling to the families, we investigated the molecular etiology of nonsyndromic deafness in 103 unrelated school children attending Nantong School for the Deaf and Mute in Jiangsu Province, China. The coding exon of the GJB2 gene was PCR amplified and sequenced. Sixty two GJB2 mutant alleles were identified in 35.9% (37/103) of the patients. Twenty five patients carried two pathogenic mutations and 12 patients carried one mutant allele. The 235delC was the most common mutation accounting for 69.4% (43/62) of GJB2 mutant alleles. The GJB2 mutant alleles accounted for 30.1% (62/206) of all chromosomes responsible for nonsyndromic hearing impairment. Testing of the 3 most prevalent deleterious frame shift mutations in this cohort detected 100% of all GJB2 mutant alleles. These results demonstrate that an effective genetic testing of GJB2 gene for patients and families with nonsyndromic hearing impairment is possible. | DAI Pu1*, YOU Yi-wen2*, CUI Jing-hong2*, YU Fei1, HAN Bing 1, KANG Dong-yang1, YUAN Hui-jun1, HAN Dong-yi1, 1. Department of Otolaryngology, PLA General Hospital, Beijing, People’s Republic China, 100853 2. Department of Otolaryngology, Nantong University Affiliated Hospital, Nantong, Jiangsu Province, People’s Republic China, 226001 *Pu Dai, Yiwen You, Jinghong Cui contribute equally to this paper | 2006 | Journal of Otology2006，1，2： | 10 |
| 9 | Glycylproline dipeptidyl aminopeptidase isoenzyme in diagnosis of primary hepatocellular carcinoma显示文摘AIM: To investigate the role of glycylproline dipeptidyl aminopeptidase (GPDA) isoenzyme in the diagnosis of primary hepatocellular carcinoma (PHC), especially in patients with negative alpha-fetoprotein (AFP).METHODS: A stage gradient polyacrylamide gel discontinuous electrophoresis system was developed to separate serum GPDA isoenzymes, which were determined in 102 patients with PHC, 45 cases with liver cirrhosis, 24cases with chronic hepatitis, 35 cases with benign liver spaceoccupying lesions, 20 cases with metastatic liver cancer and 50 cases with extra-hepatic cancer, as well as 80 healthy subjects. The relationships between GPDA isoenzymes and AFP, the sizes of tumors, as well as alanine aminotransferase (ALT) were also analyzed.RESULTS: Serum GPDA was separated into two isoenzymes,GPDA-S and GPDA-F. The former was positive in all subjects,while the latter was found mainly in majority of PHC (85.3 %)and a few cases with liver cirrhosis (11.1%), chronic hepatitis (33.3 %), metastatic liver cancer (15.0 %) and non-hepatic cancer (16.0 %). GPDA-F was negative in all healthy subjects and patients with benign liver space-occupying lesions,including abscess, cysts and angioma. There was no correlation between GPDA-F and AFP concentration or tumor size. GPDA-F was consistently positive and not correlated with ALT in PHC, but GPDA-F often converted to negative as decline of ALT in benign liver diseases. The electrophoretic migration of GPDA-F became sluggish after the treatment of neuraminidase.CONCLUSION: GPDA-F is a new useful serum marker for PHC. Measurement of serum GPDA-F is helpful in diagnosis of PHC, especially in patients with negative AFP. GPDA-F is one kind of glycoproteins rich in sialic acid. | Run-Zhou Ni Jie-Fei Huang Ming-Bing Xiao Mei Li Xian-Yong Meng Department of Gastroenterology,Affiliated Hospital of Nantong Medical College,Nantong 226001,Jiangsu Province,China | 2003 | World Journal of Gastroenterology2003，9，4： | 10 |
| 10 | Dog sciatic nerve gap repaired by artificial tissue nerve graft显示文摘The feasibility of repairing dog sciatic nerve damage by using a biodegradable artificial tissue nerve graft enriched with neuroregenerating factors is investigated. The artificial nerve graft was implanted to a 30 mm gap of the sciatic nerve damage in 7 dogs. The dogs with the same nerve damage that were repaired by interposition of the autologous nerve or were given no treatment served as control group 1 or 2, respectively. The observations include gross and morphological observations, immune reaction, electrophysiological examination, fluorescence tracing of the neuron formation and the number of the neurons at the experimental sites, etc. Results showed that 6 months after the implantation of the graft, the regenerated nerve repaired the damage of the sciatic nerve without occurrence of rejection and obvious inflammatory reaction in all 7 dogs, and the function of the sciatic nerve recovered with the nerve conduction velocity of (23.91±11.35)m/s. The regenerated neurons and the forming of axon could be observed under an electron microscope. This proves that artificial tissue nerve graft transplantation can bridge the damaged nerve ends and promote the nerve regeneration. | GU Xiaosong, ZHANG Peiyun, WANG Xiaodong, DING Fei, PENG Luping and CHENG Hongbing(Nantong Medical College, The Key Laboratory of Neuroregeneration of Jiangsu Province, Nantong 226001, China) | 2003 | Progress in Natural Science:Materials International2003，13，8： | 6 |
| 11 | Comparison of Hypolipidemic Effect of Refined Konjac Meal With Several Common Dietary Fibers and Their Mechanisms of Action显示文摘The effects of RKM in comparison with pectin, algin and agar on lipid levels in serum and liver and on liver histopathology in rats were studied. In addition, the effects of all the tested materials on the composition and output of fecal bile acid were observed. All four kinds of dietary fiber were given at a level of 5% of diet to young male rats of Wistar strain fed on a lipid-rich diet contalning 5 % lard, 1% cholesteral and 0. 25 % cholate. All the dietary fibers tested have similar effects on serum lipid composition. In all groups, these substances prevent ed increases in total cholesterol in fasting serum, but the level of triglyceride was tmchangd.The concentrations of totaI cholesterol and triglyceride in the liver were lower in the RKM group than in the control group and the other three groups. Hepatic histopathological exami nation also showed the most significant lipotropic effect in the RKM group. The daily output of fecal bile acids (CDCA+GDCA) was significantly increased in the four experimental groups than in the normal group and the control group. The increase of CDCA was more significant than GDCA, suggesting that the increase of fecal bile acids, especially CDCA, may be one of the mechanisms by which RKM and the other three dietary fibers exerts a hypocholesterolemic | WU JIE AND PENG SHU-SHENG (Department of Nutrition and Food Hygiene, Nantong Medical College, Nantong City, 226001, Jiangsu, China Department of Nutrition and Food Hygiene, West China University of Medical Sciences,Chengdu, Sichuan, China) | 1997 | Biomedical and Environmental Sciences1997，10，1： | 5 |
| 12 | Isolation and differentiation of neural stem/progenitor cells from fetal rat dorsal root ganglia显示文摘To find a promising alternative to neurons or schwann cells (SCs) for peripheral nerve repair applications,this study sought to isolate stem cells from fetal rat dorsal root ganglion (DRG) explants.Molecular expression analysis confirmed neural stem cell characteristics of DRG-derived neurospheres in terms of expressing neural stem cell-specific genes and a set of well-defined genes related to stem cell niches and glial fate decision.Under the influence of neurotrophic factors,bFGF and NGF,the neurospheres gave rise to neurofilament-expressing neurons and S100-expressing Schwann cell-like cells by different pathways.This study suggests that a subpopulation of stem cells that reside in DRGs is the progenitor of neurons and glia,which could directly induce the differentiation toward neurons,or SCs. | GU Yun1,2,HU Nan2,LIU Jie2,DING Fei2 & GU XiaoSong2* 1Medical School of Soochow University,Suzhou 215123,China 2Jiangsu Key Laboratory of Neuroregeneration,Nantong University,Nantong 226001,China | 2010 | Science China(Life Sciences)2010，53，9： | 4 |
| 13 | Abnormal expression of hepatomas and circulating telomerase and its clinical values显示文摘BACKGROUND: Hepatocellular carcinoma (HCC) is one of the most common malignant tumours worldwide. Te-lomerase is reactivated in various types of malignant tumors and may contribute to the development of HCC. To evaluate the role of telomerase in formation and development of HCC, we analyzed its expression status in different parts of HCC tissues and peripheral blood mono-nuclear cells (PBMCs) , and explored its clinical implications for diagnosis of HCC. METHODS: Total RNAs and telomerase were extracted from HCC and their non-cancerous tissues, and both relationships were analyzed between them. The expression of telomerase reverse transcriptase (hTERT) mRNA and telomerase activities in liver tissues and PBMCs were detected by RT-PCR and telomeric repeat amplification protocol (TRAP)-ELISA, respectively. The diagnostic values of telomerase in PBMCs were investigated in the diagnosis and differentiation of HCC. RESULTS: The specific activities of telomerase were 18.25 ±15.02 A/μg RNA in HCC tissues, and significantly higher than those in their non-cancerous tissues (8.16 ± 6.22 A/μg RNA, P <0. 05). But total RNA levels in HCC were 12.40 ±7.34 μg/mg wet liver and lower than 53.77 ± 52.02 μg/mg wet liver in their non-cancerous parts (P < 0.01). The different telomerase levels could be detected in PBMCs from patients with chronic liver diseases and control group. The enzyme activities were significantly higher in HCC than in any other groups (P <0.01). However, circulating telomerase levels were more obviously decreased in HCC patients after transcatheter arterial embolization treatment than before the treatment (P <0.01). The analysis of combined serum alpha-fetoprotein level and PBMCs telomerase was more sensitive and specific for the diagnosis of HCC. CONCLUSION: The abnormal expression of telomerase in HCC tissues and circulating PBMCs could be a useful marker to the diagnosis and prognosis of HCC. | Wei Wu, Deng-Fu Yao, Li-Wei Qiu, Xin-Hua Wu, Ming Yao, Xiao-Qin Su and Li Zou Nantong, China Research Center of Clinical Molecular Biology, Affiliated Hospital, Nantong University, Nantong 226001, China | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，4： | 1 |
| 14 | Application of Luxol Fast Blue staining in locating the corticospinal tract in adult rats显示文摘BACKGROUND: There are many methods for myelin staining,mordant,or the special reaction of osmic acid with lipoid is used according to different principles.The commonly used methods are classic Well staining ,classic lithium carbonate-haematine staining,fast green staining,silver staining ,etc.Luxol Fast Blue can brightly stain myelin sheath,and has certain specificity .The background can be very clean if there is proper differentiation,whereas Luxol Fast Blue is cheap and convenient to operate,thus it is an ideal staining reagent for routine myelin sheath.OBJECTIVE: To show the coricospinal tract of normal adult rats with Luxol Fast Blue shaining method.DESIGN:A repetitive measurement design.SETTINGS: Institute of Nuerobiology,Nantong University;Department of Rehabilitation Medicine,Affiliated Hospital of Nantong University.MATERIALS: Six healthy adult male SD rats of clean dergree,weighing averagely 300 g.were provided by the experimental animal center of Nantong University.1 g/L Luxol Fast Blue solution was provided by Sigma Company;Leica CM1900 cryostat microtome by Leica Company;Leica DMR microscope by Leica Company.METHODS:The experiment was carried out in the Staff Room of Human Anatomy,Nantong University in May 2005.The rats were given intraperitoneal injection of combined anesthetic(2 mL/kg),then the chest was open for perfusing saline and phosphate buffer containing formamint via heart. Brain and spinal cord were removed after 1 hour then fixed,then changed to phosphate buffer(pH 7.4)containing 300 g/L saccharu at 4 ℃.and stayed overnight,tissue blocks at pyramid,decussation of pyramid and cervical,thoracic,lumbar and sacral segments of spinal cord were removed to prepare continuous horizontal frozen sections(30 μm) after sedimentation,the sections were dried at room temperature.The corticospinal tract of normal adult rats were shown with Luxol Fast Blue staining method,and observed under Leica DMR microscope.MAIN OUTCOME MEASURES:Positive fibers in Luxol Fast Blue staining.RESULTS:After the Luxol Fast Blue staining,the labeled myelinated nerve fibers were bright blue.They located in the pyramid,decussation of pyramid and the ventral part of posterior funiculus in cervical,thoracic,lumbar and sacral segments of spinal cord.CONCLUSION:Luxol Fast Blue staining method may manifest the distribution of corticospinal tract with clear distinct in adult rats. | Su Liu1, Guangyu Shen1, Guangming Lü2, Xiaosong Gu3 1Department of Rehabilitation Medicine, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China 2Department of Human Anatomy, Nantong University, Nantong 226001, Jiangsu Province, China 3Jiangsu Key Laboratory of Nerve Regeneration, Nantong 226001, Jiangsu Province, China | 2006 | Neural Regeneration Research2006，1，7： | 1 |
| 15 | Protein expression profile in the differentiation of rat bone marrow stromal cells into Schwann cell-like cells显示文摘During the last decade,increasing evidence suggested that bone marrow stromal cells(MSCs) have the potential to differentiate into neural lineages.Many studies have reported that MSCs showed morphological changes and expressed a limited number of neural proteins under experimental conditions.However,no proteomic studies on MSCs differentiated into Schwann cell-like cells have been reported.In this study,we isolated MSCs from adult Sprague-Dawley rat femur and tibia bone marrows and induced the cells in vitro under specific conditions.By using two-dimensional gel electrophoresis(2-DE),we compared the protein profiles of MSCs before and after induced differentiation.We obtained 792 protein spots in the protein profile by 2-DE,and found that 74 spots changed significantly before and after the differentiation using PDQuest software,with 43 up-regulated and 31 down-regulated.We analyzed these 74 spots by a matrix assisted laser desorption ionization-time of flight mass spectrometry(MALDI-TOF-MS) and by database searching,and found that they could be grouped into various classes,including cytoskeleton and structure proteins,growth factors,metabolic proteins,chaperone proteins,receptor proteins,cell cycle proteins,calcium binding proteins,and other proteins.These proteins also include neural and glial proteins,such as BDNF,CNTF and GFAP.The results may provide valuable proteomic information about the differentiation of MSCs into Schwann cell-like cells. | LI WenTing1,SUN HuaLin2,XU ZengLu1,DING Fei2 & GU XiaoSong2 1 Department of Anatomy,Histology and Embryology,Peking Union Medical College,Institute of Basic Medical Science,Chinese Academy of Medical Sciences,Beijing 100005,China 2 Jiangsu Key Laboratory of Neuroregeneration,Nantong University,Nantong 226001,China | 2009 | Science China(Life Sciences)2009，52，3： | 1 |
| 16 | Heterosis of Slaughter Performance and Meat Quality of New Zealand White Rabbit,Fujian Yellow Rabbit and Hybrid Groups显示文摘[Objective]The aim was to evaluate heterosis of hybridization groups of New Zealand White rabbits and Fujian Yellow rabbits. [Method] Totaling 80 rabbits,including New Zealand White rabbits,Fujian Yellow rabbits,and direct-cross and reciprocal cross rabbits were measured in slaughter performance and meat quality. [Result] Live-weight,evisceration weight and semi-evisceration weight of rabbits in four groups were 1 817. 20 -2 308. 60,914. 10 -1 231. 50 and 985. 99 -1 315. 20 g,respectively. For slaughter performance,FY × NZ was the highest among male rabbits and NZ was the highest among female rabbits. In addition,shear forces of crureus and longissimusdorsi for rabbits in four groups were 25. 11 -40. 44 and 18. 02 -26. 44 N,respectively,among which male NZ was the lowest in shear force of crureus and female NZ was the lowest of longissimusdorsi. Water-loss rates of crureus and longissimusdorsi were 33. 43% -41. 52% and 31. 79% -39. 01%,among which male NZ proved the lowest in water-loss of crureus and male FY achieved the lowest level for the rate of longissimusdorsi. [Conclusion]Hybrid groups showed some heteroses in slaughter performance and meat quality. | XU Shi-yong 1 ,LIU Chun 2 ,FENG Kai 3 ,ZHAO Jian-ya 4 ,WU Xin-sheng 3 1. Nantong Center for Animal Disease Control and Prevention,Nantong 226006,China 2. Experimental Animal Center,Nantong University, Nantong 226001,China 3. College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China 4. School of Public Health,Nantong University,Nantong 226019,China | 2012 | Animal Husbandry and Feed Science2012，4，2： | 0 |
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