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| 1 | Gene expression profiling of MYC-driven tumor signatures in porcine liver stem cells by transcriptome sequencing显示文摘AIM:To identify the genes induced and regulated by the MYC protein in generating tumors from liver stem cells.METHODS:In this study,we have used an immortal porcine liver stem cell line,PICM-19,to study the role of c-MYC in hepatocarcinogenesis.PICM-19 cells were converted into cancer cells(PICM-19-CSCs)by overexpressing human MYC.To identify MYC-driven differential gene expression,transcriptome sequencing was carried out by RNA sequencing,and genes identified by this method were validated using real-time PCR.In vivo tumorigenicity studies were then conducted by injecting PICM-19-CSCs into the flanks of immunodeficient mice.RESULTS:Our results showed that MYC-overexpressing PICM-19 stem cells formed tumors in immunodeficient mice demonstrating that a single oncogene was sufficient to convert them into cancer cells(PICM-19-CSCs).By using comparative bioinformatics analyses,we have determined that>1000 genes were differentially expressed between PICM-19 and PICM-19-CSCs.Gene ontology analysis further showed that the MYCinduced,altered gene expression was primarily associated with various cellular processes,such as metabolism,cell adhesion,growth and proliferation,cell cycle,inflammation and tumorigenesis.Interestingly,six genes expressed by PICM-19 cells(CDO1,C22orf39,DKK2,ENPEP,GPX6,SRPX2)were completely silenced after MYC-induction in PICM-19-CSCs,suggesting that the absence of these genes may be critical for inducingtumorigenesis.CONCLUSION:MYC-driven genes may serve as promising candidates for the development of hepatocellular carcinoma therapeutics that would not have deleterious effects on other cell types in the liver. | Rajagopal N Aravalli Neil C Talbot Clifford J Steer | 2015 | World Journal of Gastroenterology2015，21，7： | 3 |
| 2 | Transanal total mesorectal excision:a valid option for rectal cancer?显示文摘Low anterior resection can be a challenging operation, especially in obese male patients and in particular after radiotherapy. Transanal total mesorectal excision(Ta TME) might offer technical advantages over laparoscopic or open approaches particularly for tumors in the distal third of the rectum. The aim of this article is to review the current experience with Ta TME. The limits and future developments are also explored. Although the experience with Ta TME is still limited, it might be a promising alternative to laparoscopic TME, especially for difficult cases where laparoscopy is too demanding. The preliminary data on complications and short-term oncological outcomes are good, but also emphasize the importance of careful patient selection. Finally, there is a need for large-scale trials focusing on long-term outcomes and oncological safety before widespread adoption can be recommended. | Nicolas C Buchs Gary A Nicholson Frederic Ris Neil J Mortensen Roel Hompes | 2015 | World Journal of Gastroenterology2015，21，41： | 3 |
| 3 | Catalase HPII from Escherichia coli Exhibits Enhanced Resistance to Denaturation显示文摘 | Switala J O'Neil J O Loewen P C | 1999 | Biochemistry1999，38，13： | 1 |
| 4 | Inhibition of NOTCH signaling by gamma secretase inhibitor engages the RB pathway and elicits cell cycle exit in T-Cell acute lymphoblastic leukemia cells显示文摘 | RAO S S O'NEIL J LIBERATOR C D | 2009 | Cancer Res2009，69，： | 1 |
| 5 | Oxygen iso- tope fractionation between synthetic aragonite and water: in-fluence of temperature and Mgz+ concentration显示文摘 | Kim S-T O'Neil J R Hillaire-Mareel C | 2007 | Geochimica et Cosmochimica Acta2007，71，： | 1 |
| 6 | Oxygen isotope fractionation between synthetic aragonite and water: Influence of temperature and Mg2 concentration 显示文摘 | KIM S T O' NEIL J R HILLAIRE-MARCEL C MUCCI A | 2007 | Geochimica Et Cosmochimica Acta2007，71，19： | 1 |
| 7 | Hydrochlori- nation of acetylene using gold catalysts = A study of cat- alyst deactivation 显示文摘 | Bongani N Neil J C Hutchigs G J | 1991 | Journal of Catalysis1991，，128： | 1 |
| 8 | Soybean aphid pred- ators and their use in integrated pest management 显示文摘 | Rutledge C E O'Neil R J Fox T B | 2004 | Ann En- tomol Soc Am2004，97，： | 1 |
| 9 | Understanding the Char- acteristics of Quality for Software Engineering Processes : A Grounded Theory Investigation 显示文摘 | TRENT A K NEIL J D STEPHEN C C | 2014 | Information and Software Technology2014，56，2： | 1 |
| 10 | Haemodynamic monitoring with pulse - induced contour cardiac output (PiCCO) in critical care显示文摘 | Ros C Neil M David J | 2003 | Intensive and Critical Care Nursing2003，8，12： | 1 |
| 11 | An innovative steel/concrete construction system 显示文摘 | HUGH BOWERMAN NEIL COYLE CHAPMAN J C | 2002 | The Structural Engineer2002，80，21： | 1 |
| 12 | The role of long lived free radicals in the ageing of irradiated ultra high molecular weight polyethylene显示文摘 | O'Neil P O Birknshaw C Leahy J J | 1999 | Polym Degradation Stability1999，63，1： | 1 |
| 13 | Normal brain in hu- man newborns: apparent diffusion coefficient and diffusion anisotropy measured by using diffusion tensor MR imaging显示文摘 | Neil J J Shiran S I McKinstry R C | 1998 | Radiology1998，209，1： | 1 |
| 14 | Soybean aphid predators and their use in integrated pest management 显示文摘 | RUTLEDGE C E O'NEIL R J FOX T B | 2004 | Ann Entomol Soc Am2004，97，2： | 1 |
| 15 | Optimal timing of wound drain re-moval following total joint arthroplasty 显示文摘 | Drinkwater C J Neil M J | 1995 | J Arthroplasty1995，10，2： | 1 |
| 16 | Nitric oxide prevents leukocxyte adherence role of superoxide显示文摘 | Gaboury J Richard C W Neil G | 1993 | Am J Physiol1993，265，： | 1 |
| 17 | Tranexamic acid for intracerebral haemorrhage within 2 hours of onset: protocol of a phase Ⅱ randomised placebo-controlled double-blind multicentre trial显示文摘Rationale Haematoma growth is common early after intracerebral haemorrhage(ICH),and is a key determinant of outcome.Tranexamic acid,a widely available antifibrinolytic agent with an excellent safety profile,may reduce haematoma growth.Methods and design Stopping intracerebral haemorrhage with tranexamic acid for hyperacute onset presentation including mobile stroke units(STOP-MSU)is a phase Ⅱ double-blind,randomised,placebo-controlled,multicentre,international investigator-led clinical trial,conducted within the estimand statistical framework.Hypothesis In patients with spontaneous ICH,treatment with tranexamic acid within 2 hours of onset will reduce haematoma expansion compared with placebo.Sample size estimates A sample size of 180 patients(90 in each arm)would be required to detect an absolute difference in the primary outcome of 20%(placebo 39%vs treatment 19%)under a two-tailed significance level of 0.05.An adaptive sample size re-estimation based on the outcomes of 144 patients will allow a possible increase to a prespecified maximum of 326 patients.Intervention Participants will receive 1 g intravenous tranexamic acid over 10 min,followed by 1 g intravenous tranexamic acid over 8 hours;or matching placebo.Primary efficacy measure The primary efficacy measure is the proportion of patients with haematoma growth by 24±6 hours,defined as either≥33%relative increase or≥6 mL absolute increase in haematoma volume between baseline and follow-up CT scan.Discussion We describe the rationale and protocol of STOP-MSU,a phase Ⅱ trial of tranexamic acid in patients with ICH within 2 hours from onset,based in participating mobile stroke units and emergency departments. | Nawaf Yassi Henry Zhao Leonid Churilov Bruce C V Campbell Teddy Wu Henry Ma Andrew Cheung Timothy Kleinig Helen Brown Philip Choi Jiann-Shing Jeng Annemarei Ranta Hao-Kuang Wang Geoffrey C Cloud Rohan Grimley Darshan Shah Neil Spratt Der-Yang Cho Karim Mahawish Lauren Sanders John Worthington Ben Clissold Atte Meretoja Vignan Yogendrakumar Mai Duy Ton Duc Phuc Dang Nguyen Thai My Phuong Huy-Thang Nguyen Chung Y Hsu Gagan Sharma Peter J Mitchell Bernard Yan Mark W Parsons Christopher Levi Geoffrey A Donnan Stephen M Davis | 2022 | Stroke & Vascular Neurology2022，7，2： | 1 |
| 18 | Treatment of chronic painful diabetic neu- ropathy with isosorbide dinitrate spray 显示文摘 | Kevin C J Neil R Gerry R | 2003 | Diabetes Care2003，25，10： | 1 |
| 19 | Treatment of chronic painful diabetic neuropathy with isosorbide dinitrate spray显示文摘 | Kevin C J Neil R Gerry R | | 0，，10： | 1 |
| 20 | Measurement of the percentage volume particle size distribution of powdered microcrystalline cellulose using reflectance near-infrared speetroscopy显示文摘 | O'NEIL A J JEER D MOFFAT A C | 2003 | Analyst2003，128，： | 1 |
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