| 12 | Effects of Epidermal Growth Factor and β-mercaptoethanol on In-vitro Fertilization and Development of Oocytes from Hormone-Stimulated Lambs显示文摘[Objective]The aim was to explore optimization of system of oocytes in-vitro culture of young animals. [Method]Effects of EGF ( epidermal growth factor) and β-mercaptoethanol in maturation media on fertilization,cleavage and blastaea were researched,which were then compared with those of adult sheep. [Result]EGF in different concentrations had little effects on rates of cleavage and blastaea ( P >0.05) and β-mer-captoethanol in different concentrations would improve blastaea rate of oocytes,for example,100 μmol/L of β-mercaptoethanol has significant effects on balstaea rate ( P <0.05) ,but has little effects on cleavage rate ( P >0. 05) . In addition,rates of cleavage and balstaea of oocytes in lamb were both lower than those of adult sheep ( P <0.05) ; fertilization rate of oocytes in lamb ( P <0.05) ,which differed little with that of adult sheep ( P >0.05) ,could be significantly enhanced by 100 μmol/L of β-mercaptoethanol. Furthermore,polyspermy rate was higher than that of adult sheep without β-mercaptoethanol ( P <0.05) ; the rate was of little differences with that of adult sheep with 100 μmol/L of β-mercaptoethanol ( P > 0.05) ; unfertilization rate ( 20%) in media without β-mercaptoethanol was a little higher ( P >0.05) than those of adult sheep ( 12.3%) and those in media with β-mercaptoethanol ( 13.5%) . [Conclusion]Developmental capacity of oocytes and fertilization rate could be improved by 100 μmol/L of β-mercaptoethanol with polyspermy rate reduced,but developmental capacity of lamb was significantly lower than that of adult sheep. | GUO Hong1,2,3,WAN Peng-cheng2,3SHI Wen-yan4,NI Jian-hong2,3,DAI Rong2,3,MAO Qing-qing2,3,5,ZHU Hui1,2,3,ZHANG Bin2,3,LIU Chang-bin2,3,SHI Guo-qing2,3 1. College of Life Sciences,Shihezi University,Shihezi 832000,China 2. Animal Husbandry and Veterinary Institute,Xinjiang Academy of Agri- cultural and Reclamation Science,Shihezi 832000,China 3. Key Lab of Sheep Breeding and Reproduction,Xinjiang Agroreclamation Academy of Sciences,Shihezi 832000,China 4. Medical College,Shihezi University,Shihezi 832000,China 5. College of Animal Science & Technolo- gy,Nanjing Agricultural University,Nanjing 210095,China | 2012 | Animal Husbandry and Feed Science2012，4，2： | 0 |
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