|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Comparison of phenotypic markers and neural differentiation potential of multipotent adult progenitor cells and mesenchymal stem cells显示文摘AIM: To compare the phenotypic and neural differentiation potential of human bone marrow derived multipotent adult progenitor cells (MAPC) and mesenchymal stem cells (MSC). METHODS: Cultures of MAPC and MSC were established in parallel from same samples of human bone marrow (n = 5). Both stem cell types were evaluated for expression of pluripotency markers including Oct-4 and Nanog by immunocytochemistry and reversetranscription polymerase chain reaction (RT-PCR) and expression of standard mesenchymal markers including CD14, CD34, CD44, CD45, CD73, CD90, CD105 andhuman leukocyte antigen (HLA)-ABC by flow cytometry. After treatment with neural induction medium both MAPC and MSC were evaluated for expression of neural proteins [neuronal filament-200 (NF-200) and glial fibrillar acidic protein (GFAP)] by immunocytochemistry and Western blotting and neural genes [NF-200, GFAP, Tau, microtubule-associated protein (MAP)-1B, MAP-2, neuron-specific enolase (NSE) and oligodendrocyte-1 (Olig-1)] by quantitative real-time-PCR. RESULTS: MAPC had small trigonal shaped while MSC had elongated spindle-shaped morphology. The MAPC expressed Oct-4 and Nanog both at gene and protein levels, whereas MSC were negative for these pluripotent markers. MAPC were negative for HLA-ABC while MSC had high expression of HLA-ABC. In addition, MAPC as compared to MSC had significantly lower expression of CD44 (36.56% ± 1.92% vs 98.23% ± 0.51%), CD73 (15.11% ± 2.24% vs 98.53% ± 2.22%) and CD105 (13.81% ± 3.82%vs 95.12% ± 5.65%) (P < 0.001, for all) MAPC cultures compared to MSC cultures treated with neural induction medium had significantly higher fold change expression of NF-200 (0.64), GFAP (0.52), Tau (0.59), MAP-2 (0.72), Olig-1 (0.18) and NSE (0.29) proteins (P < 0.01 for Olig-1 and P < 0.001 for rest) as well as higher fold change expression of genes of NF-200 (1.34),GFAP (1.12),Tau (1.08),MAP-1B (0.92), MAP-2 (1.14) andNSE (0.4) (P < 0.001 for all). CONCLUSION: MAPC can be differentially characterized from MSC as Oct-4 and Nanog positive stem cells with no expression of HLA-ABC and low expression of mesenchymal markers CD44, CD73 and CD105 and when compared to MSC they possess greater predilection for differentiation into neuro-ectodermal lineage. | Saurabh Pratap Singh Naresh Kumar Tripathy Soniya Nityanand | 2013 | World Journal of Stem Cells2013，5，2： | 10 |
| 2 | Expression of interferon-gammaand tumor necrosis factor-alpha in bone marrow T cells and their levelsin bone marrow plasma in patients with aplastic anemia显示文摘 | Dubey S Shukla P Nityanand S | 2005 | Ann He-matol2005，84，9： | 1 |
| 3 | An Analysis of RadiM Segregation for Different Sized Spherical Solids in Rotary Cylinders 显示文摘 | Nityanand N Manley B Henein H | 1986 | Metallurgical Transactions B1986，17，2： | 1 |
| 4 | Cytokine mRNA repertoire of peripheral blood mononuclear cells in Takayasu's arteritis显示文摘 | Tripathy NK Chauhan SK Nityanand S | 2004 | Clin Exp Immunol2004，138，： | 1 |
| 5 | Expression of interferon-gamma and tumor necrosis factor-alpha in bone marrow T cells and their levels in bone marrow plasma in patients with aplastic anemia显示文摘 | DUBEY S SHUKLA P NITYANAND S | 2005 | Ann Hematol2005，84，： | 1 |
| 6 | Clinical and serologicalcharacterization of autoimmune hemolytic anemia in a tertiary caerhospital North lndia显示文摘 | Das S S Nityanand S Chaudhary R | 2009 | Ann Hemato丨2009，88，8： | 1 |
| 7 | Synthesis and physicochemical characterization of nanocrys-talline cobalt doped lanthanum strontium ferrite 显示文摘 | NITYANAND C NALIN W B RAJKUMAR B S | 2011 | SolidState Sci2011，13，： | 1 |
| 8 | A bias in the alphabeta t cell receptor variable region gene usage in Takayasu's arteritis显示文摘 | Nityanand S Giscombe R Srivastava S | 1997 | Clin Exp Immunol1997，107，： | 1 |
| 9 | Ret- rope ritonealapproach to abdominal aortic aneurysm repair preserves splanchnicperfusion as measured by gastric tonometry 显示文摘 | Nityanand Arya Muhammad Anees Sharif Luk Louis Lau | 2010 | Annals of Vascular Surgery2010，24，3： | 1 |
| 10 | Expanded T eel populations in patients with Wegener' s granulomatosis: characteristics and correlates with disease activity显示文摘 | Giscombe R Nityanand S I_ewin N | 1998 | J Clin Immunol1998，18，： | 1 |
| 11 | Clinical and serological characterization of autoimmune hemolytic anemia in a tertiary caer hospital North India显示文摘 | Das SS Nityanand S Chaudhary R | 2009 | Ann Hematol2009，88，8： | 1 |
| 12 | Interactions between GIPC–APPL and GIPC–TRP1 regulate melanosomal protein trafficking and melanogenesis in human melanocytes显示文摘 | Rajendra Kedlaya Gokul Kandala Tie Fu Liu Nityanand Maddodi Sulochana Devi Vijayasaradhi Setaluri | 2011 | Archives of Biochemistry and Biophysics2011，，2： | 1 |
| 13 | Platelets in essential hypertension显示文摘 | Nityanand S Pande I Bajpai VK | 1993 | Thromb Res1993，72，5： | 1 |
| 14 | Expression of interferon gamma and tumor necrosis factor alpha in bone marrow T cells and their levels in bone marrow plasma in patients with aplastie anemia显示文摘 | Dubey S Shukla P Nityanand S | 2005 | Ann Hematol2005，84，9： | 1 |
| 15 | Anti-annexin V antibodies in Takayasu's arteritis;prevalence and relationship with disease activity显示文摘 | Tripathy NK Sinha N Nityanand S | 2003 | Clin Exp Immunol2003，134，： | 1 |
| 16 | Antimonocyte antibodies in Takayasu's arteritis:prevalence of and relation to disease activity显示文摘 | Tripathy NK Sinha N Nityanand S | 2003 | Journal Rheumatol2003，30，： | 1 |
| 17 | Microbial Laccases and their applica- tions : A Review 显示文摘 | Desai S S Nityanand C | 2011 | Asian Journal of Biotechnology2011，3，2： | 1 |
| 18 | Expression of interferon-gamma and tumor necrosis factor-alpha in bone marrow T cells and their levels in bone marrow plasma in patients with aplastic anemia显示文摘 | Dubey S Shukla P Nityanand S | 2005 | Ann Hematol2005，84，9： | 1 |
| 19 | Expression of interferon-gamma and tumor necrosis factor-alpha in bone marrow T cells and their levels in bone marrow plasma in patients with aplastic anemia显示文摘 | DUBEY S SHUKLA P NITYANAND S | 2005 | Ann Hematol2005，84，： | 1 |
| 20 | Antibodies against cardiolipin and oxidatively modified LDL, in 50-year-old men predict myocardial infarction显示文摘 | Wu RH Nityanand S Berglund L | 1997 | Arterioscler Thromb Vasc Biol1997，17，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员