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| 1 | miRNA expression profiles in cerebrospinal fluid and blood of patients with Alzheimer’s disease and other types of dementia-an exploratory study显示文摘Background:MicroRNAs(miRNAs)are small non-coding RNA molecules that function as posttranscriptional regulators of gene expression.Measurements of miRNAs in cerebrospinal fluid(CSF)and blood have just started gaining attention as a novel diagnostic tool for various neurological conditions.The purpose of this exploratory investigation was to analyze the expression of miRNAs in CSF and blood of patients with Alzheimer’s disease(AD)and other neurodegenerative disorders in order to identify potential miRNA biomarker candidates able to separate AD from other types of dementia.Methods:CSF was collected by lumbar puncture performed on 10 patients diagnosed with AD and 10 patients diagnosed with either vascular dementia,frontotemporal dementia or dementia with Lewy bodies.Blood samples were taken immediately after.Total RNA was extracted from cell free fractions of CSF and plasma,and a screening for 372 known miRNA sequences was carried out by real time quantitative polymerase chain reactions(miRCURY LNA™Universal RT miRNA PCR,Polyadenylation and cDNA synthesis kit,Exiqon).Results:Fifty-two miRNAs were detected in CSF in at least nine out of ten patients in both groups.Among these,two miRNAs(let-7i-5p and miR-15a-5p)were found significantly up-regulated and one miRNA(miR-29c-3p)was found significantly down-regulated in patients with AD compared to controls.One hundred and sixty-eight miRNAs were frequently detected in the blood,among which miR-590-5p and miR-142-5p were significantly up-regulated and miR-194-5p was significantly down-regulated in AD patients compared to controls.Conclusions:Detection of miRNA expression profiles in blood and in particular CSF of patients diagnosed with different types of dementia is feasible and it seems that several expressional differences between AD and other dementia types do exist when measured in a clinically relevant setup.In this explorative pilot study,the deregulated miRNAs in CSF of AD patients may be associated with relevant target genes related to AD pathology,including APP and BACE1,which suggests that miRNAs are interesting candidates for AD biomarkers in the future. | Sofie Sølvsten Sørensen Ann-Britt Nygaard Thomas Christensen | 2016 | Translational Neurodegeneration2016，5，1： | 14 |
| 2 | Mechanical stresses in carotid plaques using MRI based fluid structure interaction mod- els显示文摘 | Kock S A Nygaard J y Eldrup N | 2008 | J Bomech2008，41，8： | 1 |
| 3 | Full-Scale Mea- surements of SO2 Gas Phase Concentrations and Slurry Compositions in a Wet Flue Gas Desulphurisation Spray Absorber 显示文摘 | Nygaard H G Kiil S Johnson J E | 2004 | Fuel2004，83，11： | 1 |
| 4 | The effect of culinary preparation on carbohydrate composition, texture and sensory quality of Jerusalem artichoke tubers ( Helianthus tuberosus L.)显示文摘 | Vibe Bach Sidsel Jensen Ulla Kidmose J?rn Nygaard S?rensen Merete Edelenbos | 2013 | LWT - Food Science and Technology2013，，1： | 1 |
| 5 | Recovery of sensory nervefibres surgical decompression in diabetes in lumbar radiculopathy use of quantitative sensory testing in the expooration of different populations of nerve fibres显示文摘 | Nygaard O P Kloster R Mellgren S I | 1998 | J Neurol Neurosurg Psychiart1998，64，： | 1 |
| 6 | 查看详情显示文摘 | Schuch R Garibian A Nygaard P Raymond S Araik G Hans H S Patrick J P Per N | | 0，，： | 1 |
| 7 | Factors determining performance of bronchodilator reversibility tests in middle-aged and elderly显示文摘 | Lehmann S Vollset SE Nygaard HA | 2004 | Respir Med2004，98，11： | 1 |
| 8 | Full fluid-solid cohesive finite-element model to simulate near wellbore fractures显示文摘 | SALEHI S NYGAARD R | 2015 | Journal of Energy Resources TechnologyTransactions of the ASME2015，137，01： | 1 |
| 9 | Trophic importance of Laminaria hyperborea to kelp forest consumers and the importance of bacterial degradation to food quality显示文摘 | Norderhaug K M Fredriksen S Nygaard K | 2003 | Marine Ecology- Progress Series2003，255，： | 1 |
| 10 | Radioiodine therapy for multinodular toxic goiter显示文摘 | Nygaard B Hegedǖs L Ulriksen P | 1999 | Arch Intern Med1999，159，12： | 1 |
| 11 | Pelvic Organ Pro- lapse in the Women' Health Initiative: Gravity and Gravidity 显示文摘 | Hendrix S L Clark A Nygaard I | 2002 | Am J Obstet Gynecol2002，186，6： | 1 |
| 12 | Dopa-responsive dystonia-long-ternl treatment response and prognosis 显示文摘 | Nygaard TG Marsden CD Fahn S | 1991 | Neurology1991，41，21： | 1 |
| 13 | Dynamic asset allocation under mean-reverting returns, stocbastic interest rates and inflation uncertainty- Are popular recommendations consistent with rational behavior? 显示文摘 | Claus Munk Carsten S?rensen Tina Nygaard Vinther | 2004 | International Review of Economics and Finance2004，2004，： | 1 |
| 14 | Tracking techno-science networks : A case study of fuel cells and related hy- drogen technology R&D in Norway 显示文摘 | Klitkou A Nygaard S Meyer M | 2007 | Scientometrics2007，70，2： | 1 |
| 15 | Pelvic organ prolapse in the Women' s Health Initiative: Gravity and gravidity 显示文摘 | Hendrix S L Clark A Nygaard I | 2002 | Am J Obstet Gyneco12002，186，06： | 1 |
| 16 | Identification and analysis of miRNAs in human breast cancer and teratoma samples using deep sequencing显示文摘 | NYGAARD S JACOBSEN A LINDOW M | 2009 | BMC Med Genomics2009，2，： | 1 |
| 17 | Pelvic or- gan prolapse in the Women'S Health Initiative: gravity and gravidity显示文摘 | HENDRIX S L CLARK A NYGAARD 1 | 2002 | Am J Obstet Gynecol2002，186，6： | 1 |
| 18 | Preoperative radiotherapy prolongs survival in operable esophageal carcinoma: a randomized, muhicenter study of preoperative radiotherapy and chemotherapy-the second Scandinavian trial in esophagus cancer 显示文摘 | Nygaard K Hagen S Hansen HS | 1992 | World J Surg1992，16，： | 1 |
| 19 | Pelvic organ pro- lapse in the Women' Health Initiative:gravity and gravidi- ty显示文摘 | Hendrix S L Clark A Nygaard I | 2002 | Am J Obstet Gynaeeol2002，186，： | 1 |
| 20 | Single-walled and multi-walled carbon nanotubes promote allergic immune responses in mice显示文摘 | NYGAARD U C HANSEN J S SAMUELSEN M | 2009 | Toxicol Sci2009，109，： | 1 |
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