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| 1 | Lentivirus介导神经营养因子-3促进脊髓损伤大鼠功能恢复显示文摘目的探讨Lentivirus介导分泌神经营养因子-3(NT-3)直接体内转基因治疗大鼠脊髓损伤作用和机制。方法将28只Wistar大鼠在T10水平制成半横断损伤模型,随机分为体内转基因治疗组和损伤对照组,每组14只;用携带NT-3和绿色荧光蛋白(GFP)的Lentivirus对大鼠行直接体内转基因治疗;荧光显微镜观察体内转基因表达,后肢运动功能评分法(BBB评分)检测大鼠后肢功能恢复情况。结果用荧光显微镜检测到损伤脊髓内有较多的持续表达转基因的细胞;BBB评分结果显示,从伤后第4周开始,实验组大鼠后肢功能较对照组明显恢复(P<0.01),至第10周时,实验组和对照组BBB分差增大,达3.3分。结论Lentivirus是一种有效的转基因载体,由Lentivirus介导分泌NT-3的直接体内转基因治疗能够明显促进损伤脊髓的功能恢复。 | 蔡培强 汤逊 林月秋 Oudega Mart 徐林 Blits B 阳运康 M.OUDEGA | 2005 | 中华创伤杂志2005，21，7： | 2 |
| 2 | Adeno-associated viral vectormediated neurotrophin gene transfer in the injured adult rat spinal cord improves hind-limc function显示文摘 | Blits B Oudega M Boer GJ | 2003 | Neurosci2003，118，1： | 1 |
| 3 | Adeno-aasociated viral vector-mediated neurotrophin gene transfer in the injured adult rat spinal cord improves hind~limb function显示文摘 | Blits B Oudega M Boer GJ | 2003 | Neuroscience2003，118，： | 1 |
| 4 | Adeno-associated viral vector-mediated neurotrophin gene transfer in the injured adult rat spinal cord improves hind-limb function显示文摘 | Blits B Oudega M Boer GJ Bartlett Bunge M Verhaagen J | | 0，，： | 1 |
| 5 | The F4 fimbrial antigen of Escherichia coli and its' receptors 显示文摘 | Van den Broeck W Cox E Oudega B | 2000 | Vet Microbiol2000，71，： | 1 |
| 6 | Adeno-Associated viral vector-mediated neurotrophin gene transfer in the injured adult rat spinal cord improves hind-limb function 显示文摘 | Blits B Oudega M Boer GJ | 2003 | Neuroscience2003，118，1： | 1 |
| 7 | The F4 fimbrial antigen of Escherichia coli and its receptors 显示文摘 | Broeck W V Cox E Oudega B | 2000 | Vet Microb2000，71，： | 1 |
| 8 | mrp, a rnultigenc, rnultifunctional locus in Bacillus subtilis with roles in resistance to cholate and to Na+ and in pH homeostasis显示文摘 | Ito M Guffanti AA Oudega B | 1999 | Journal of Bacteriology1999，181，8： | 1 |
| 9 | Analysis of the type Ⅳ fimbrial-subunit gene fimA of Xanthomonas hyacinthi:application in PCR-mediated detection of yellow disease in hyacinthis显示文摘 | van Doorn J Hollinger T C Oudega B | 2001 | Appl Environ Microbiol2001，67，2： | 1 |
| 10 | The F4 fim- brial antigen of Escherichia coli and its receptors显示文摘 | Van den Broeck W Cox E Oudega B | 2000 | Veterinary Microbiology2000，71，34： | 1 |
| 11 | Molecular and structural aspects of fimbriae biosynthesis and assembly in Escherichia coli显示文摘 | MOL O OUDEGA B | 1996 | FEMS Microbiol Rev1996，19，1： | 1 |
| 12 | Binding of bovine transferrin by Pasteurella multocida serotype B: 2, 5, a strain which causes haemorrhagic septicaemia in buffalo and cattle 显示文摘 | VEKEN J W OUDEGA B LUIRINK J | 1994 | FEMS Microbiol Lett1994，115，23： | 1 |
| 13 | Adeno-associated viral vector-mediated neurotrophin gene transfer in the injured adult rat spinal cord improves hind-limb function显示文摘 | Blits B Oudega M Boer GJ | 2003 | Neuroscience2003，118，： | 1 |
| 14 | Conduction of impulses by axons regenerated in a Schwann cell graft in the transected adult rat thoracic spinal cord显示文摘 | Pinzon A Calancie B Oudega M | 2001 | J Neurosci2001，64，5： | 1 |
| 15 | The Escherichia coli K99 periplasmic chaperone FanE is a monomeric protein显示文摘 | Mol O Fokkema H Oudega B | 1996 | FEMS Microbiol Lett1996，138，223： | 1 |
| 16 | Analysis of the type Ⅳ fimbrial-subunit gene fimA of Xanthomonas hyacinthi:application in PCR-mediated detection of yellow disease in hyacinths显示文摘 | Doorn J Van Hollinger T C Oudega B | 2001 | Appl Environ Microbiol2001，67，2： | 1 |
| 17 | 基因工程化分泌神经营养因子-3的鼠胚神经干细胞实验研究显示文摘目的:探索以Lentivirus为载体,构建同时表达绿色荧光蛋白(GFP)和神经营养因子-3(NT-3)的基因工程化鼠胚神经干细胞(NSC)的可行性。方法:体外分离培养鼠胚NSC,用同时携带NT-3和GFP的lentivirus转染构建工程化NSC;用荧光显微镜、鼠胚背根神经结培养(DorsalRootGanglion,DRG)、Westernblot等方法检测基因工程NSC的转基因表达。结果:荧光显微镜观察到几乎100%的工程化NSC表达GFP;DRG培养和Westernblot检测到基因工程化NSC能高效分泌NT-3蛋白。结论:以Lentivirus为载体,构建同时携带并稳定表达GFP和NT-3的基因工程化鼠胚NSC是可行的,可为脊髓损伤基础研究提供有价值的细胞资源。 | 蔡培强 汤逊 林月秋 Blits B Oudega M 阳运康 徐林 周田华 | 2006 | 中国康复医学杂志2006，21，5： | 1 |
| 18 | Neurotrophic factors, cellular bridges and gene therapy for spinal cord injury显示文摘 | JONES L L OUDEGA M BUNGE M B | 2001 | J Physiol2001，533，1： | 1 |
| 19 | Long-term effects of methylprednisolone follwing transaction of adult rat spinal cord显示文摘 | Oudega M Vagas C G Weber A B | 1999 | Eur J Neurosci1999，11，7： | 1 |
| 20 | The F4 fimbrial antigen of Escherichia coli and its receptors 显示文摘 | van den Broeck W Cox E Oudega B | 2000 | Veterinary Microbiology2000，71，： | 1 |
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