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您的检索式：作者名="Pablo Smircich"
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| 1 | Trypanosoma cruzi : Molecular characterization of an RNA binding protein differentially expressed in the parasite life cycle显示文摘 | Leticia Pérez-Díaz María Ana Duhagon Pablo Smircich José Sotelo-Silveira Carlos Robello Marco Aurelio Krieger Samuel Goldenberg Noreen Williams Bruno Dallagiovanna Beatriz Garat | 2007 | Experimental Parasitology2007，，： | 1 |
| 2 | Evidence for a negative feedback control mediated by the 3′ untranslated region assuring the low expression level of the RNA binding protein TcRBP19 in T. cruzi epimastigotes显示文摘 | Leticia Pérez-Díaz Lucía Pastro Pablo Smircich Bruno Dallagiovanna Beatriz Garat | 2013 | Biochemical and Biophysical Research Communications2013，，： | 1 |
| 3 | Conserved Curvature of RNA Polymerase Ⅰ Core Promoter Beyond rRNA Genes: The Case of the Tritryps显示文摘In trypanosomatids, the RNA polymerase I(RNAPI)-dependent promoters controlling the ribosomal RNA(r RNA) genes have been well identified. Although the RNAPI transcription machinery recognizes the DNA conformation instead of the DNA sequence of promoters, no conformational study has been reported for these promoters. Here we present the in silico analysis of the intrinsic DNA curvature of the r RNA gene core promoters in Trypanosoma brucei, Trypanosoma cruzi, and Leishmania major. We found that, in spite of the absence of sequence conservation, these promoters hold conformational properties similar to other eukaryotic r RNA promoters. Our results also indicated that the intrinsic DNA curvature pattern is conserved within the Leishmania genus and also among strains of T. cruzi and T. brucei. Furthermore, we analyzed the impact of point mutations on the intrinsic curvature and their impact on the promoter activity. Furthermore, we found that the core promoters of protein-coding genes transcribed by RNAPI in T. brucei show the same conserved conformational characteristics. Overall, our results indicate that DNA intrinsic curvature of the r RNA gene core promoters is conserved in these ancient eukaryotes and such conserved curvature might be a requirement of RNAPI machinery for transcription of not only r RNA genes but also protein-coding genes. | Pablo Smircich María Ana Duhagon Beatriz Garat | 2015 | Genomics, Proteomics & Bioinformatics2015，13，6： | 0 |
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