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| 1 | Immunocytochemical identification and localization of APUD cells in the gut of seven stomachless teleost fishes显示文摘AIM To study the cell types,localization,distribution density and morphology of APUDcells in the intestinal mucosa of stomachlessteleost fishes.METHOD By using the peroxidase-antiperoxidase complex(PAP)immunocytochemical staining technique theidentification,localization and morphology ofimmunoreactive(IR)endocrine cells seattered inthe intestinal mucosa of grass carp(Cyenopharyngodon idellus),black carp(Mylopharyngodon piceus)and common carp(Cyprinus carpio)were investigated with 20kinds of antisera prepared against mammalianpeptide hormones of APUD cells,and likewise byusing avidin-biotin-peroxidase complex(ABC)method those of silver carp(Hypophthalmichthys molitrix),bighead(Aristichthys nobilis),silver crucian carp(Carassius gibelio)and bluntnose black bream(Megalobrama amblyocephala)were alsostudied with 5 different antisera.Thereplacement of the first antiserum by phosphatebuffered saline(PBS)was employed as a control.IR endocrine cells were counted with asquare-mesh ocular micrometer from 10 fieldsselected randomly in every section of each partof the intestine specimen.The average numberof IR endocrine cells per mm2 was counted toquantify their distribution density.RESULT Gastrin(GAS)-,Gastric inhibitorypeptide(GIP)-,glucagon(GLU)-,glucagon-likeimmunoreactants(GLI)-,bovine pancreaticpolypeptide(BPP)-,leucine-enkephalin(ENK)-and substance P(SP)-IR endocrine cells werefound in the gut of grass carp,black carp andcommon carp,and somatostatin(SOM)-IRendocrine cells were only seen in common carp.GAS-,GIP-and GLU-IR endocrine cells werefound in the intestinal mucosa of silver carp,bighead,silver crucian carp and bluntnose blackbream.Most of IR endocrine cells had the higherdistribution density in the foregut and midgut,and were longer in shape.They had a long apicalcytoplasmic process extended to the gut lumenand a basal process extended to adjacent cellsor basement membrane and touched with it.Sometimes,the basal cytoplasmic processformed an enlarged synapse-like structure in thecontiguous part with basement membrane.Thisphenomenon provided new morphologicalevidence for neuroendocrine and paracrinesecretory function of these enteroendocrinecells.CONCLUTION At least 8 kinds of IR endocrinecells were found in the gut of stomachlessteleost species for the first time in China.TheseIR endocrine cells scattering in the gut mucosabelong to the APUD system.Among them,thehormones secreted by SP-,ENK-,SOM-and GLU-IR endocrine cells belong to the peptides of dualdistribution in the brain and gut.This providednew evidence for the concept of brain-gutpeptide.According to the cell types,distribution density,morphologicalcharacteristics and variety in shape of APUDcells in the gut of stomachless teleost fishes,itis deemed that the digestive tract of fishes isalso an endocrine organ of great importance andcomplexity. | Pan QS Fang ZP Zhao YX | 2000 | World Journal of Gastroenterology2000，6，1： | 25 |
| 2 | Magnetic resonance imaging and biological makers in pituitary adenomas with invasion of the cavernous sinus space显示文摘 | Chen ZP Liu YS | 2005 | J Neurooncol2005，74，： | 1 |
| 3 | Magnetic resonance imaging and biological markers in pituitary adenomas with invasion of the cavernous sinus space显示文摘 | Pan LX Chen ZP Liu YS | 2005 | J Neurooncol2005，74，1： | 1 |
| 4 | A powerful hybrid puc operon promoter tightly regulated by both of IPTG and low oxygen level 显示文摘 | Hu ZL Zhao ZP Pan Y Tu Y Chen GP | 2010 | Biochemistry2010，75，： | 1 |
| 5 | Magnetic resonance imaging and biological markers in pituitary adenomas with invasion of the cavernous sinus space显示文摘 | Pan LX Chen ZP Liu YS | 2005 | J Neurooncol2005，74，1： | 1 |
| 6 | Magnetic resonance imaging and biological markers in pituitary adenomas with invasion of the cavernous sinus space显示文摘 | Pan LX Chen ZP Liu YS | 2005 | J Neurooncol2005，74，1： | 1 |
| 7 | Analysis of 7cases with colon lipoma显示文摘 | CHEN ZP PAN QX ZHUANG JL | 2002 | Chinese Misdiagnosis Journal2002，2，1： | 1 |
| 8 | Altered expression of claudin -3 and claudin -4 in ectopic endometrium of women with endometriosis 显示文摘 | Pan XY Li X Weng ZP | 2009 | Fertil Steril2009，91，5： | 1 |
| 9 | Antitumor efficacy of SarCNU in a human glioma xenograft model expressing both MGMT and extraneuronal monoamine transporter 显示文摘 | Chen ZP Pan J Huang Q | 2001 | Neuro Oncol2001，1，1： | 1 |
| 10 | Phenotypic plasticity rather than locally adapted ecotypes allows the invasive alligator weed to colonize a wide range of habitats显示文摘 | Geng YP Pan XY Xu CY Zhang WJ Li B Chen JK Lu BR Song ZP | 2007 | Biol Invasions2007，9，3： | 1 |
| 11 | Microsomal triglyceride transfer protein gene -493 G/T polymorphism and its association with serum lipid levels in Bama Zhuang long-living families in China 显示文摘 | Pan SL Luo XQ Lu ZP | 2012 | Lipids Health Dis2012，11，： | 1 |
| 12 | Simultaneous tracking and stabilization of mobile robots:An adaptive approach显示文摘 | Do KD Jiang ZP Pan J | 2004 | IEEE Transactions on Automatic Control2004，49，7： | 1 |
| 13 | A global output-feedback controner for simultaneous tracking and stabilization of unicycle-type mobile robots 显示文摘 | Do KD Jiang ZP Pan J | 2004 | IEEE Transactions on Robotics and Automation2004，20，3： | 1 |
| 14 | Magnetic resonance imaging and biological markers in pituitary adenomas with invasion of the cavernous sinus space显示文摘 | Pan LX Chen ZP Liu YS | 2005 | J Neurooncol2005，74，1： | 1 |
| 15 | Correlation of tumor markers p53,bcl-2,CD34,CD44H,CD44v6,and Ki-67 with survival and metastasis in laryngeal squamous cell carcinoma显示文摘 | Spafford MF Koeppe J Pan ZP | 1996 | Arch Otolaryngol Head Neck Surg1996，18，7： | 1 |
| 16 | An algorithm for risk assessment and intervention of mother to child transmission of hepatitis B virus显示文摘 | Pan CQ Duan ZP Bhamidimarri KR | 2012 | Clin Gastroenterol Hepatol2012，10，5： | 1 |
| 17 | Magnetic resonance imaging and biological markers in pituitary adenomas with invasion of the cavernous sinus space 显示文摘 | Pan LX Chen ZP Liu YS | 2005 | J Neuroonco12005，74，1： | 1 |
| 18 | Cholesteryl ester transferprotein TaqlB polymorphism and its association with ser-um lipid levels and longevity in Chinese Bama Zhuangpopulation显示文摘 | Pan SL Wang F Lu ZP | 2012 | Lipids Health Dis2012，11，： | 1 |
| 19 | Determination of free digoxin in sera of 8 patiens with chronic cardiac insufficiency显示文摘 | Li JH Pan H Wu ZP | 1995 | Acta Pharmacol Sin1995，16，1： | 1 |
| 20 | Magnetic resonance imaging and biological markers in pituitary adenomas with invasion of the cavernous sinus space 显示文摘 | Pan LX Chen ZP Liu YS | 2005 | J Neurooncol2005，74，1： | 1 |
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