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| 1 | A deep learning-enabled portable imaging flow cytometer for cost-effective, highthroughput, and label-free analysis of natural water samples显示文摘We report a deep learning-enabled field-portable and cost-effective imaging flow cytometer that automatically captures phase-contrast color images of the contents of a continuously flowing water sample at a throughput of 100 mL/h.The device is based on partially coherent lens-free holographic microscopy and acquires the diffraction patterns of flowing micro-objects inside a microfluidic channel.These holographic diffraction patterns are reconstructed in real time using a deep learning-based phase-recovery and image-reconstruction method to produce a color image of each micro-object without the use of external labeling.Motion blur is eliminated by simultaneously illuminating the sample with red,green,and blue light-emitting diodes that are pulsed.Operated by a laptop computer,this portable device measures 15.5 cm×15 cm×12.5 cm,weighs 1 kg,and compared to standard imaging flow cytometers,it provides extreme reductions of cost,size and weight while also providing a high volumetric throughput over a large object size range.We demonstrated the capabilities of this device by measuring ocean samples at the Los Angeles coastline and obtaining images of its micro-and nanoplankton composition.Furthermore,we measured the concentration of a potentially toxic alga(Pseudo-nitzschia)in six public beaches in Los Angeles and achieved good agreement with measurements conducted by the California Department of Public Health.The cost-effectiveness,compactness,and simplicity of this computational platform might lead to the creation of a network of imaging flow cytometers for largescale and continuous monitoring of the ocean microbiome,including its plankton composition. | Zoltán Gӧrӧcs Miu Tamamitsu Vittorio Bianco Patrick Wolf Shounak Roy Koyoshi Shindo Kyrollos Yanny Yichen Wu Hatice Ceylan Koydemir Yair Rivenson Aydogan Ozcan | 2018 | Light(Science & Applications)2018，7，1： | 14 |
| 2 | Genetic Variation in IL28B Is Associated With Chronic Hepatitis C and Treatment Failure: A Genome-Wide Association Study显示文摘 | Andri Rauch Zoltán Kutalik Patrick Descombes Tao Cai Julia Di Iulio Tobias Mueller Murielle Bochud Manuel Battegay Enos Bernasconi Jan Borovicka Sara Colombo Andreas Cerny Jean–Fran?ois Dufour Hansjakob Furrer Huldrych F. Günthard Markus Heim Bernard Hirs | 2010 | Gastroenterology2010，，4： | 2 |
| 3 | 查看详情显示文摘 | Schuch R Garibian A Nygaard P Raymond S Araik G Hans H S Patrick J P Per N | | 0，，： | 1 |
| 4 | Pathogenesis of endometriosis显示文摘 | Annemiek W N Patrick G G Ayse Y D | 2004 | Best Pract Res Clob2004，18，2： | 1 |
| 5 | Optimizing the use of anthracyelines in older patients with breast cancer显示文摘 | Patrick G Morris Monica N | 2011 | Critical Reviews in Oncology/Hematology2011，77，2： | 1 |
| 6 | Multiple independent emer- gences of type 2 vaccine-derived polioviruses during a large outbreak in northern Nigeria显示文摘 | Burns C C Shaw J Jorba J Bukbuk D Adu F Gumede N Pate M A Abanida E A Gasasira A Iber J Chen Q Vincent A Chenoweth P Henderson E Wannemuehler K Naeem A Umarni R N Nishimura Y Shimizu H Baba M Adeniji A Williams A J Kil- patrick D R Oberste M S Wassilak S G Tomori O Pallansch M A Kew O | 2013 | J Virol2013，87，9： | 1 |
| 7 | Sources of and Responses to Risk:Factor Analyses of Large-scale US Combelt Farmers显示文摘 | Musser W N | 1997 | Manaholt Studies1997，，7： | 1 |
| 8 | Sources of and Responses to Risk: Factor Analyses of Large-scale US Cornbeh Farmers 显示文摘 | Patrick G F Musser W N | 1997 | Manshoh Studies1997，7，： | 1 |
| 9 | Conversion of p35 to p25Deregulates cdk5 activity and promotes neurodegeneration显示文摘 | Patrick G N Zukerberg L Nikolic M et a1 | 1999 | Nature1999，402，6762： | 1 |
| 10 | Impact of an unsealed forest road stream crossing: water quality and sediment sources 显示文摘 | Patrick N J L G J Sheridan | 2002 | Hydrology Process2002，16，： | 1 |
| 11 | Clinical and eco-nomic outcomes from a community hospital,s antimicrobial stew-ardship program显示文摘 | ANURAG N MALANI MD PATRICK G | 2013 | Am J Infect Control2013，41，2： | 1 |
| 12 | Extraction of proteins from plant tissues for two-dimensional electrophoresis analysis显示文摘 | PATRICK G ECKHARD N HANS L | 2003 | Electrophoresis2003，24，1： | 1 |
| 13 | Direct yaw moment control actuated through electric drivetrains and fric- tion brakes: Theoretical design and experimental assessment显示文摘 | LEONARDO D N ALDO S PATRICK G | 2015 | Mechatronics2015，26，： | 1 |
| 14 | Self-assembly structures of nonionic surfactants at graphite/solution interfaces显示文摘 | Gregory G Warr Srinivas Manne | 1997 | Langmuir1997，13，： | 1 |
| 15 | Genetic Variation in IL28B Is Associated With Chronic Hepatitis C and Treatment Failure: A Genome-Wide Association Study显示文摘 | Andri Rauch Zoltán Kutalik Patrick Descombes Tao Cai Julia Di Iulio Tobias Mueller Murielle Bochud Manuel Battegay Enos Bernasconi Jan Borovicka Sara Colombo Andreas Cerny Jean–Fran?ois Dufour Hansjakob Furrer Huldrych F. Günthard Markus Heim Bernard Hirs | 2010 | Gastroenterology2010，，4： | 1 |
| 16 | Human Paragonimiasis After Eating Raw or Undercooked Crayfish - Missouri, July 2006-September 2010显示文摘 | Patrick S L Turabelidze G Marx H Grim A Lane M A Weil G J Bailey T C ?nen N F Demertzis L M Tuteur P G Hayes E V Davila S Z Folk S M Mitchem R E Kammerer E Fannon L P Jones J L Wilkins P P Lo Y C | 2010 | MMWR. Morbidity and Mortality Weekly Report2010，，： | 1 |
| 17 | New Synthetic pathways from Gibberellins to Antheridiogens isolated from the Fern Genus Anemia 显示文摘 | FURBER M MANDER L N PATRICK G L | 1990 | J Org Chem1990，55，： | 1 |
| 18 | Enhanced resistance to helicoverpa zea in tobacco expressing an activated form of Maize ribosome-inactivating protein显示文摘 | PATRICK F D WEI N Z JEFFERY W G | 2003 | Journal of Agricultural and Food Chemistry2003，51，： | 1 |
| 19 | Genetic Variation in IL28B Is Associated With Chronic Hepatitis C and Treatment Failure: A Genome-Wide Association Study显示文摘 | Andri Rauch Zoltán Kutalik Patrick Descombes Tao Cai Julia Di Iulio Tobias Mueller Murielle Bochud Manuel Battegay Enos Bernasconi Jan Borovicka Sara Colombo Andreas Cerny Jean–Fran?ois Dufour Hansjakob Furrer Huldrych F. Günthard Markus Heim Bernard Hirs | 2010 | Gastroenterology2010，，4： | 1 |
| 20 | Conversion of p35 to p25 deregulates cdk5 Activity and promotes neurodegeneration显示文摘 | ZUKERBERG L NIKOLIC M | 1999 | Nature1999，402，： | 1 |
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