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| 1 | Role of CD56-expressing immature biliary epithelial cells in biliary atresia显示文摘AIM: To analyze the clinical and pathological parameters and expression of the neural cell adhesion molecule(CD56) in patients with biliary atresia(BA).METHODS: Established clinical laboratory markers of hepatic function, including enzyme activity, protein synthesis, and bilirubin metabolism, were evaluated in patients with BA and compared with those in patients with choledochal cysts and neonatal hepatitis. Pathological changes in tissue morphology and fibrosis were examined by histological and tissue collagen staining. Immunohistochemical staining for the biliary epithelial cell markers CD56 and CK19 together with the Notch signaling related molecules Notch1 and Notch2 was performed in the context of alterations in the structure of intrahepatic biliary ducts.RESULTS: Differences in some clinical laboratoryparameters among the three diseases examined were observed, but they did not correlate with the pathological classification of fibrosis in BA. Immunohistochemical staining showed the presence of CD56-positive immature bile ducts in most patients(74.5%) with BA but not in patients with choledochal cysts or neonatal hepatitis. The number of CD56-expressing cells correlated with disease severity, with more positive cells present in the later stages of liver damage(81.8% vs 18.2%). Furthermore, bile plugs were mainly found in CD56-positive immature biliary ducts. Notch signaling was a key regulatory pathway in biliary duct formation and played a role in tissue fibrosis. Notch1 was co-expressed in CD56-positive cells, whereas Notch2 was found exclusively in blood vessels in the portal area of patients with BA. CONCLUSION: The maturation of biliary epithelial cells and the expression of Notch may play a role in the pathogenesis of BA. | Rui-Zhong Zhang Jia-Kang Yu Jiao Peng Feng-Hua Wang Hai-Ying Liu Vincent CH Lui John M Nicholls Paul KH Tam Jonathan R Lamb Yan Chen Hui-Min Xia | 2016 | World Journal of Gastroenterology2016，22，8： | 8 |
| 2 | Downregulation of Hes1 expression in experimental biliary atresia and its effects on bile duct structure显示文摘AIM To analyze the expression and function of the notchsignaling target gene Hes1 in a rhesus rotavirusinduced mouse biliary atresia model. METHODS The morphologies of biliary epithelial cells in biliary atresia patients and in a mouse model were examined by immunohistochemical staining. Then, the differential expression of Notch signaling pathway-related molecules was investigated. Further, the effects of the si RNAmediated inhibition of Hes1 expression were examined using a biliary epithelial cell 3 D culture system.RESULTS Both immature(Ep CAM+) and mature(CK19+) biliary epithelial cells were detected in the livers of biliary atresia patients without a ductile structure and in the mouse model with a distorted bile duct structure. The hepatic expression of transcripts for most Notch signaling molecules were significantly reduced on day 7 but recovered to normal levels by day 14, except for the target molecule Hes1, which still exhibited lower m RNA and protein levels. Expression of the Hes1 transcriptional co-regulator, RBP-Jκ was also reduced. A 3 D gel culture system promoted the maturation of immature biliary epithelial cells, with increased expression of CK19+ cells and the formation of a duct-like structure. The administration of Hes1 si RNA blocked this process. As a result, the cells remained in an immature state, and no duct-like structure was observed.CONCLUSION Our data indicated that Hes1 might contribute to the maturation and the cellular structure organization of biliary epithelial cells, which provides new insight into understanding the pathology of biliary atresia. | Rui-Zhong Zhang Xin-Hao Zeng Ze-Feng Lin Ming-Fu Yan-Lu Tong Vincent CH Lui Paul KH Tam Jonathan R Lamb Hui-Min Xia Yan Chen | 2018 | World Journal of Gastroenterology2018，24，29： | 5 |
| 3 | 牛体内重组牛生长激素(rbST)的快速高灵敏度检测显示文摘重组牛生长激素(rbST)在一些国家和地区常被作为猪或牛的生长促进剂(用于乳牛可以增加牛奶产量),但在欧盟国家,有法规明确禁止生长激素的使用。重组牛生长激素本身是蛋白质,在生物基质中的浓度较低,且样品基质十分复杂,对其进行有效分析还存在一定难度,因而,长久以来其分析方法一直局限为酶联免疫检测法。 | Gaud Pinel Sandrine Roulet-Rochereau ylvain Chéreau Fabrice Monteau Bruno Le Bizec Paul Silcock Marie-Hélène Le Breton | 2010 | 食品安全导刊2010，，8： | 2 |
| 4 | CT of Dry Skulls with Craniofacial Deformi ties:Accuracy of Three-Dimensional Reconstruction显示文摘 | David CH Paul LT | 1985 | Radiology1985，157，1： | 1 |
| 5 | Ideal and perfect hierar- chical secret sharing schemes 显示文摘 | Tentu A N Paul P Venkaiah V Ch | 2013 | IACR Cryptology ePrint Archive2013，，： | 1 |
| 6 | Using data clustering to improve cleaning performance for flash memory显示文摘 | Mei-Ling Chiang Paul CH Lee Ruei-Chuan Chang | 1999 | Software Practice and Experience1999，29，3： | 1 |
| 7 | Effect of ionic valence and electronic correlations on the thermoelectric power in some filled skutterudites 显示文摘 | Bauer E Berger St Paul Ch | 2003 | Physica B2003，328，12： | 1 |
| 8 | A new t*ridoscof>i(、stagingsystem for angiofibromas 显示文摘 | Snyderman CH Paul H Carrau KL | 2010 | Arch Otolaryngol Head Neck Surg2010，136，6： | 1 |
| 9 | Visualization of coronary tree and detection of coronary artery stenosis using 16-slice, sub-millimeter computed tomography: preliminary experience显示文摘 | Paul JF Ohanessian A Caussin Ch | 2004 | Arch Mal Coeur Vaiss2004，97，1： | 1 |
| 10 | Ideal and perfect hier- archical secret sharing schemes显示文摘 | Tentu A N Paul P Venkaiah V Ch | 2013 | IACR Cryptology ePrint Archive2013，，： | 1 |
| 11 | New markers of inflammation and endothelial cell activation显示文摘 | Paul E Szmitko BS Wang CH | 2003 | Circulation2003，108，19： | 1 |
| 12 | CT of dry skulls with craniofacial deformities: accuracy of three dimensional reconstruction显示文摘 | David CH Paul LT | 1985 | Radiology1985，157，1： | 1 |
| 13 | Phase field model of solidification in binary alloys: capillarity and solute trapping effects 显示文摘 | Charach Ch Paul C F | 1999 | Journal of Crystal Growth1999，，2： | 1 |
| 14 | CT of dry skulls with craniofacial deformities:Accuracy of three-dimensional reconstruction显示文摘 | David CH Paul LT | 1985 | Radiology1985，157，1： | 1 |
| 15 | Isobolographic analysis of non-depolarising muscle relaxant interactions at their receptor site 显示文摘 | Paul M Kindler CH Fokt RM | 2002 | Eur J Pharmacol2002，438，： | 1 |
| 16 | Characterization of the interactions between volatile anesthetics and neuromuscular blockers at the muscle nicotinic acetylcholine receptor显示文摘 | PAUL M FOKT RM KINDLER CH | 2002 | Anesth Analg2002，95，2： | 1 |
| 17 | Role for stearoyl-CoA desaturase-1 in leptin-mediated weight loss显示文摘 | Paul CH Makoto MZ Nicholas DS | 2002 | Science2002，297，： | 1 |
| 18 | Neurologic complications in adult living donor liver transplant patients: an underestimated factor?显示文摘 | Fuat Hakan Saner Julia Gensicke Steven W. M. Olde Damink Goran Pavlakovi? Juergen Treckmann Marc Dammann Gernot M. Kaiser Georgios C. Sotiropoulos Arnold Radtke Susanne Koeppen Susanne Beckebaum Vito Cicinnati Silvio Nadalin Massimo Malagó Andreas Paul Ch | 2010 | Journal of Neurology2010，，2： | 1 |
| 19 | Erythropoietin in renaltrnsplant recipients:stdiesbased on recombinant human erythropoietion显示文摘 | Ward HJ Sun CH Paul WL, | 1989 | Trans Proceedings1989，21，： | 1 |
| 20 | Adrenal my-elolipoma-report of a case显示文摘 | Chakraborty J Paul P Ch Gumta M K | 2006 | J Indian Med Assoc2006，104，3： | 1 |
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