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| 1 | Study of the effect of atorvastatin on the interaction between ICAM-1 and CD11b by live-cell single-molecule force spectroscopy显示文摘The interaction between the cell adhesion molecule CD11b and its ligand ICAM-1 plays an important role in inflammatory responses in the disease of atherosclerosis. Atorvastatin is a commonly prescribed statin drug which has been considered as one of the most potent therapeutic agents for atherosclerosis due to its lipid-lowering effect. Recently, there is a growing body of evidence that atorvastatin has anti-inflammatory effect. We have applied the advanced method of live-cell single-molecule force spectroscopy to investigate the effect of atorvastatin on adhesion force between ICAM-1 and CD11b. Our result showed that single-molecule binding force of ICAM-1 and CD11b detected by AFM in the living cells was about 40 pN, and atorvastatin did not affect this force by blocking ICAM-1 or CD11b. This was different from the ICAM-1 monoclonal antibody, which could directly reduce the binding force of ICAM-1 and CD11b. Flow cytometry results revealed that atorvastatin pretreatment decreased the ICAM-1 expression in TNF-α activated HUVECs, which may contribute to its anti-inflammatory effect. The study provides a new approach to study anti-inflammatory mechanism for clinic drugs. | LI Yi1, SHI XiaoLi2, LIU HuiLiang1, YI ShaoQiong3, ZHANG XueJie2 & FANG XiaoHong2 1Department of Cardiology, the General Hospital of Chinese People’s Armed Police Forces, Beijing 100039, China 2Beijing National Laboratory for Molecular Sciences Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China 3State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China | 2010 | Science China Chemistry2010，53，4： | 2 |
| 2 | Studies on Human Fetal Lens Crystallins Under Oxidative Stress and Protective Effects of Tea Polyphenols显示文摘Purpose: To investigate the oxidative modification of water-soluble crystallins of human fetal lens with H2O2 and fourteen metal ions with or without EDTA. Tea-polyphenols (TP) was added to above solutions in order to testing their antioxidative abilities. Methods: The experiments were performed at 37℃ with final concentration of 2.5mg/ml protein, 0.1 mM metal ions, 0.3 mM EDTA and 1.0 mM H2O2. Then the TP was added to the solution with CuSO4 and H2O2, after 5 or 24 hours, the crystallins were analysed with SDS-PAGE and IEF.Results : There were marked oxidative modifications of lens protein in H2O2 and copper without EDTA. In SDS-PAGE patterns, we found an increase in those species above of bands higher than 30 kD and some diffuse bands from 30 to 17 kD after 5 hours. In IEF patterns, there were a general increase on acidity with loss of the more basic species. When the TP was added, there was not any difference with control group.Conclusion: The results indicate that exposure of water-soluble | Hui Xiang, Suhua Pan, Shaozhen LiGuangdong Provincial Corps Hospital, Chinese People’ s Armed Police Forces, Guangzhou 510010, China Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China | 1998 | 眼科学报1998，14，3： | 2 |
| 3 | Characterization and enrichment of cardiomyocytes derived from human embryonic stem ceils 显示文摘 | Xu C Police S Rao N | 2003 | Circ Res2003，91，6： | 1 |
| 4 | “全球化背景下高等教育特色发展模式论坛”综述显示文摘'全球化背景下高等教育特色发展模式论坛'围绕着高等教育的发展特色,高等教育如何与社会接轨、如何为社会服务,高等教育的培养目标、教育方法等热点问题进行了充分的交流。强调信息化时代对高等教育提出了新的挑战,教育要具有适应性和弹性;强调高等教育必须服务于社会需求;强调高等教育必须注重内涵建设;引入了公安教育的新视角——私人保安教育与培训,为我院公安教育的深入研究和发展提供了新的思路、拓展了新的领域。 | Dean’s Office of Liaoning Administrators College of Police and Justice (Liaoning Administrators College of Police and Justice,Shenyang,Liaoning 110161, China) | 2010 | 辽宁公安司法管理干部学院学报2010，，1： | 1 |
| 5 | Increases in striatal preproenkephalin gene expression are associated with nigrostriatal damage but not L-DOPA-induced dyskinesias in the squirrel monkey显示文摘 | Quik M Police S Langston JW | 2002 | Neuroscience2002，113，1： | 1 |
| 6 | Mechanisms of death in elderly patients with acute myocardial infarction exposed to frbrinolytic therapy显示文摘 | Polic S Rumboldt Z Novak K | 2006 | Eur Heart J2006，27，2： | 1 |
| 7 | Characterization and enrichment of cardiomyocytes derived from human embryonic stem cells显示文摘 | Xu C Police S Rao N | 2002 | Circ Res2002，91，6： | 1 |
| 8 | Characterization and enrichment of eardiomyocytes derived from human embryonic stem cells显示文摘 | Xu C Police S Rao N | 2002 | IRC Res2002，91，6： | 1 |
| 9 | Mechanisms of death inelderly patients with acute myocardial infarction exposed tofibrinolytic therapy显示文摘 | Polic S Rumboldt Z Novak K | 2006 | Eur Heart J2006，27，2： | 1 |
| 10 | Characterization and enrichment of cardiomyocytes derived from human embryonic stem cells显示文摘 | Xu C Police S Rao N | 2002 | Circ Res2002，91，： | 1 |
| 11 | Characterization and enrichment of cardiomyocytes derived from human embryonic stem cells显示文摘 | Xu C Police S Rao N | 2002 | Circ Res2002，91，6： | 1 |
| 12 | Characterization and enrichment of cardiomyocytes derived from human embryonic stem cells 显示文摘 | Xu C Police S Rao N | 2002 | Circ Res2002，91，6： | 1 |
| 13 | Characterization and enrichment of cardiomyocytes derived from human embryonic stem cells显示文摘 | Xu C Police S Rao N | | 0，，06： | 1 |
| 14 | Characterization and enrichment of cardiomyocytes derived from human embryonic stem cells 显示文摘 | Xu CH Police S Rao N | 2002 | Circulation Research2002，91，6： | 1 |
| 15 | Characterization and enrichment of cardiomyocytes derived from human embryonic stem cells显示文摘 | Police S Rao N | 2002 | Circ Res2002，91，6： | 1 |
| 16 | Characterization and enrichment of cardiomyocyte derived from human embryonic stem cells 显示文摘 | Xu C Police S Rao N et cd | 2002 | Cire Res2002，91，6： | 1 |
| 17 | Viral inhibitors of NKG2D ligands: friends or foes of immune surveillance?显示文摘 | Jonjic S Polic B Krmpotic A | 2008 | EurJ Immunol2008，38，11： | 1 |
| 18 | Immune evasion of natural killer cells by viruses 显示文摘 | Jonjic S Babic M Polic B | 2008 | Curr Opin Immunol2008，20，1： | 1 |
| 19 | Characterization and enrichment of cardiomyocytes derived from human embryonic stem cells显示文摘 | Xu CH Police S Rao N | 2002 | Circ Res2002，91，： | 1 |
| 20 | Characterization and enrichment of cardiomyocytes derived from human embryonic stem cells 显示文摘 | Xu C Police S Rao N | 2002 | Circ Res2002，91，： | 1 |
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