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| 1 | Precise in vivo genome editing via single homology arm donor mediated intron-targeting gene integration for genetic disease correction显示文摘In vivo genome editing represents a powerful strategy for both understanding basic biology and treating inherited diseases.However,it remains a challenge to develop universal and efficient in vivo genome-editing tools for tissues that comprise diverse cell types in either a dividing or non-dividing state.Here,we describe a versatile in vivo gene knock-in methodology that enables the targeting of a broad range of mutations and cell types through the insertion of a minigene at an intron of the target gene locus using an intracellularly linearized single homology arm donor.As a proof-of-concept,we focused on a mouse model of prematureaging caused by a dominant point mutation,which is difficult to repair using existing in vivo genome-editing tools.Systemic treatment using our new method ameliorated aging-associated phenotypes and extended animal lifespan,thus highlighting the potential of this methodology for a broad range of in vivo genome-editing applications. | Keiichiro Suzuki Mako Yamamoto Reyna Hernandez-Benitez Zhe Li Christopher Wei Rupa Devi Soligalla Emi Aizawa Fumiyuki Hatanaka Masakazu Kurita Pradeep Reddy Alejandro Ocampo Tomoaki Hishida Masahiro Sakurai Amy NNemeth Estrella Nunez Delicado Josep MCampistol Pierre Magistretti Pedro Guillen Concepcion Rodriguez Esteban Jianhui Gong Yilin Yuan Ying Gu Guang-Hui Liu Carlos Lopez-Otin Jun Wu Kun Zhang Juan Carlos Izpisua Belmonte | 2019 | Cell Research2019，29，10： | 2 |
| 2 | αKLOTHO and sTGFβR2 treatment counteract the osteoarthritic phenotype developed in a rat model显示文摘Dear Editor,Homeostasis and repair are critical biological processes that allow for tissue and organ preservation and function in multicellular organisms.Their regulation and extension vary drastically across the animal kingdom,and mammals show limited tissue-specific regenerative capacity that declines with age.During aging,articular cartilage is one of the tissues that undergo substantial changes in the matrix structure,molecular composition,metabolic activity,and mechanical properties(Loeser et al.2016). | Paloma Martinez-Redondo Isabel Guillen-Guillen Noah Davidsohn Chao Wang Javier Prieto Masakazu Kurita Fumiyuki Hatanaka Cuiqing Zhong Reyna Hernandez-Benitez Tomoaki Hishida Takashi Lezaki Akihisa Sakamoto Amy NNemeth Yuriko Hishida Concepcion Rodriguez Esteban Kensaku Shojima Ling Huang Maxim Shokhirev Estrella Nunez-Delicado Josep MCampistol Isabel Guillen-Vicente Elena Rodriguez-Inigo Juan Manuel Lopez-Alcorocho Marta Guillen-Vicente George Church Pradeep Reddy Pedro Guillen-Garcia Guang-Hui Liu Juan Carlos Izpisua Belmonte | 2020 | Protein & Cell2020，11，3： | 2 |
| 3 | Inter simple se- quence repeat (ISSR) polymorphism and its application in plant breeding显示文摘 | Pradeep Reddy M Sarla N Siddiq E A | 2002 | Euphytica2002，128，： | 1 |
| 4 | Role of DREB transcription factors in abiotic and biotic stress tolerance in plants显示文摘 | Pradeep K. Agarwal Parinita Agarwal M. K. Reddy Sudhir K. Sopory | 2006 | Plant Cell Reports2006，，12： | 1 |
| 5 | Geneti- cally modified mouse models for premature ovarian failure(POF) 显示文摘 | Krishna Jagarlamudi Pradeep Reddy Deepak Adhikari | 2010 | Molecular and Cellular Endocrinology2010，315，2： | 1 |
| 6 | Very high gravity(VHG)ethanolic brewing andfermentation:A research update显示文摘 | Pradeep Puligundla Daniela Smogrovicova Vijaya Sarathi Reddy Obulam | 2011 | J Ind Microbiol Biotechnol2011，38，9： | 1 |
| 7 | Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding显示文摘 | M. Pradeep Reddy N. Sarla E.A. Siddiq | 2002 | Euphytica2002，，1： | 1 |
| 8 | Inter simple sequence repeat(ISSR) polymorphism and its application in plant breeding显示文摘 | Pradeep Reddy M Sarla N Siddiq E A | 2002 | Euphytica2002，128，： | 1 |
| 9 | Inter simple sequence repeat(ISSR) polymorphism and its application in plant breeding 显示文摘 | Pradeep Reddy M Sarla N Siddiq E A | 2002 | Euphytica2002，128，： | 1 |
| 10 | Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding显示文摘 | M. Pradeep Reddy N. Sarla E.A. Siddiq | 2002 | Euphytica2002，，1： | 1 |
| 11 | Microsatellite instability and promoter hypermethylation in colorectal cancer in India显示文摘 | V. V. Ravi Kanth Sandeep Bhalsing M. Sasikala G. V. Rao R. Pradeep Urmila Steffie Avanthi D. Nageshwar Reddy | 2014 | Tumor Biology2014，，5： | 1 |
| 12 | Role of DREB transcription factors in abiotic and biotic stress tolerance in plants显示文摘 | Pradeep K. Agarwal Parinita Agarwal M. K. Reddy Sudhir K. Sopory | 2006 | Plant Cell Reports2006，，： | 1 |
| 13 | Inter-simple sequence repeat (ISSR) polymorphism and its application in plant breeding 显示文摘 | Pradeep Reddy M Sarla N Siddiq E A | 2002 | Euphytica2002，128，： | 1 |
| 14 | Robot-Assisted Endoscopic Submucosal Dissection Is Effective in Treating Patients With Early-Stage Gastric Neoplasia显示文摘 | Soo Jay Phee Nageshwar Reddy Philip W.Y. Chiu Pradeep Rebala Guduru V. Rao Zheng Wang Zhenglong Sun Jennie Y.Y. Wong Khek–Yu Ho | 2012 | Clinical Gastroenterology and Hepatology2012，，10： | 1 |
| 15 | Hepatoprotective evaluation of Anogeissus latifolia:In vitro and in vivo studies显示文摘AIM:To evaluate the hepatoprotective activity of a hydroalcoholic extract of the bark of Anogeissus latifolia; in vitro in primary rat hepatocyte monolayer culture and in vivo in the liver of Wistar rats intoxicated by carbon tetrachloride (CCl4).METHODS: In the in vitro study, a primary hepatocyte monolayer culture was treated with CCl4 and extract of Anogeissus latifolia. Hepatoprotective activity was demonstrated in the CCl4 damaged primary monolayer culture. In the in vivo study, the hepatoprotective activity of a hydroalcoholic extract of Anogeissus latifolia was analyzed in liver injured CCl4-treated rats. Biochemical parameters including serum transaminases [aspartate aminotransferase (AST) and alanine aminotransferase (ALT)] and alkaline phosphatase (ALP) in serum were analyzed. The biochemical findings were supplemented with histopathological examination of rat liver sections.RESULTS: In vitro: primary hepatocyte monolayer cultures were treated with CCl4 and extract of Anogeissus latifolia. A protective activity could be demonstrated in the CCl4 damaged primary monolayer culture. In vivo : Hydroalcoholic extract of Anogeissus latifolia (300 mg/kg) was found to have protective activity in rats with CCl4-induced liver damage as judged from serum marker enzyme activity.CONCLUSION: The above findings lead to the conclusion that the hydroalcoholic extract of Anogeissus latifolia is hepatoprotective. Hence, we suggest that the inclusion of this plant in the management of liver disorders is justified. | Hulikere Ananth Pradeep Saleemullah Khan Karamkonda Ravikumar Mohammed Fazil Ahmed Meesala Srinivasa Rao Mandava Kiranmai Dachani Sudhershan Reddy Shaik Rasheed Ahamed Mohammed Ibrahim | 2009 | World Journal of Gastroenterology2009，15，38： | 1 |
| 16 | Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding显示文摘 | Pradeep Reddy M Sarla N Siddiq E A | 2002 | Euphytica2002，128，： | 1 |
| 17 | Mechanisms maintaining the dormancy and survival of mammalian primordial follicles显示文摘 | Pradeep Reddy Wenjing Zheng Kui Liu | 2009 | Trends in Endocrinology & Metabolism2009，，2： | 1 |
| 18 | Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding显示文摘 | M. Pradeep Reddy N. Sarla E.A. Siddiq | 2002 | Euphytica2002，，1： | 1 |
| 19 | High gravity fermentation of sugarcane molasses to produce ethanol:Effect of nutrients显示文摘 | PRADEEP P REDDY O V | 2010 | Indian Journal of Microbiology2010，50，1： | 1 |
| 20 | Inter-simple se- quence repeat (ISSR) polymorphism and its applica- tion in plant breeding显示文摘 | Pradeep Reddy M Sarla N Siddiq E A | 2002 | Euphytica2002，128，： | 1 |
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