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| 1 | The Genome of Artemisia annua Provides Insight into the Evolution of Asteraceae Family and Artemisinin Biosynthesis显示文摘Artemisia annua,通常已知的同样香甜的苦恼或 Qinghao,是到中国的一个灌木土著人并且长被用于药用的目的。A。annua 现在作为有势力抗疟药混合物的唯一的生来的来源全球性被栽培, artemisinin。这里,我们报导 A 的 1.74-gigabase 染色体的一个高质量的草稿集会。annua,高度异质接合,富于重复定序,并且包含 63 ? 226 编码蛋白质的基因,在定序的植物种类之中的最大的数字之一。我们发现了那,作为在 Asteraceae 的一些定序的染色体之一, A。annua 染色体包含对这大被子植物 clade 特定的很多基因。尤其是,扩大和编码涉及萜烯生合成的酶的基因的功能的多样化与 artemisinin biosynthetic 小径的进化一致。我们进一步由介绍那 A 的 transcriptome 揭示了。annua 发展了复杂 transcriptional 规章的网络位于 \O 下面 artemisinin 生合成。把转基因的 A 基于我们产生了的全面 genomic 和 transcriptomic 分析。artemisinin 高级的生产的 annua 线,它现在为大规模生产准备好了并且将从而帮助遇见增加 artemisinin 的全球需求的挑战。 | Qian Shen Lida Zhang Zhihua Liao Shengyue Wang Tingxiang Yan Pu Shi Meng Liu Xueqing Fu Qifang Pan Yuliang Wang Zongyou Lv Xu Lu Fangyuan Zhang Weimin Jiang Yanan Ma Minghui Chen Xiaolong Hao Ling Li Yueli Tang Gang Lv Yan Zhou Xiaofen Sun Peter E. Brodelius Jocelyn K.C. Rose Kexuan Tang | 2018 | Molecular Plant2018，11，6： | 47 |
| 2 | Homology-mediated end joining-based targeted integration using CRISPR/Cas9显示文摘 | Yao, Xuan Wang, Xing Hu, Xinde Liu, Zhen Liu, Junlai Zhou, Haibo Shen, Xiaowen Wei, Yu Huang, Zijian Ying, Wenqin Wang, Yan Nie, Yan-Hong Zhang, Chen-Chen Li, Sanlan Cheng, Leping Wang, Qifang Wu, Yan Huang, Pengyu Sun, Qiang Shi, Linyu Yang, Hui | 2017 | Cell Research2017，27，6： | 28 |
| 3 | One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs显示文摘CRISPR/Cas9 系统是一个有效编辑基因的方法,但是编辑基因的动物的多数显示出 mosaicism,与编辑仅仅在房间的部分发生。这里,我们证明那单个基因或多重基因能被 Cas9 mRNA 和多重邻近的单个指南的 RNA 的 zygotic 注射完全在老鼠和猴子胚胎击倒(spaced 10-200 bp 分开) 指向仅仅一把单个钥匙每基因的 exon。个别地在 Y 染色体上跟随八基因的指向的删除的 F0 老鼠的 Phenotypic 分析在产生猛烈老鼠表明了这条途径的坚韧性。重要地,这条途径在高效率交付完全的基因大美人(Arntl 上的 100% 并且 91% 在 Prrt2 上) 在猴子胚胎。最后,我们能在一个单个步骤产生一只完全的 Prrt2 猛烈猴子,表明在很快建立编辑基因的猴子模型的这条途径的实用性。 | Erwei Zuo Yi-Jun Cai Kui Li Yu Wei Bang-An Wang Yidi Sun Zhen Liu Jiwei Liu Xinde Hu Wei Wei Xiaona Huo Linyu Shi Cheng Tang Dan Liang Yan Wang Yan-Hong Nie Chen-Chen Zhang Xuan Yao Xing Wang Changyang Zhou Wenqin Ying Qifang Wang Ren-Chao Chen Qi Shen Guo-Liang Xu Jinsong Li Qiang Sun Zhi-Qi Xiong Hui Yang | 2017 | Cell Research2017，27，7： | 21 |
| 4 | Activation of hypoxia-inducible factor-1α via nuclear factor-κB in rats with chronic obstructive pulmonary disease显示文摘积累的数据建议了可诱导的因素(HIF )-1 玩的那组织缺氧在煽动性的过程的进化和繁殖的一个重要角色。与长期的妨碍的肺的疾病(COPD ) 在老鼠描绘 HIF-1 的激活并且检验原子因素(NF ) 的可能的角色在这个过程的 -B,老鼠被 lipopolysaccharide (LPS ) 和暴露的 introtracheal 灌输质问到香烟烟。Pyrrolidine dithiocarbamate (PDTC ) 经由口头的线路被管理在 LPS 或香烟管理前的 1 h。四个星期以后,肺的功能和组织学被测试;bronchoalveolar lavage 液体(BALF ) 和动脉的血气体是 assayed。肺的 NF-B 的激活被量的 PCR, immunoblot 分析,和 electrophoretic 活动性移动估计试金分别地。结果证明 LPS 和烟雾导致了在人看见的 COPD 的特征。PDTC 在 PDTC+COPD 的 BALF 减轻了 COPD 的发展和 cytokines 的层次组显著地与 COPD 的相比被减少组。肺的 NF-B 的激活被 PDTC 禁止,在 COPD 组的 HIF-1 基因表示的累积被 PDTC 预告的处理稀释。而且, HIF-1 的 mRNA 层次指向基因 heme oxygenase-1 (HO-1 ) 和脉管的 endothelial 生长因素(VEGF ) 与由 PDTC 的 HIF-1 的变细平行。这些调查结果显示经由 NF-B 的 HIF-1 小径的激活贡献 NF-B 禁止者的 COPD,和管理的发展可以稀释 COPD 的发展。 | Hong Jiang Yesen Zhu Hui Xu Yu Sun Qifang Li | 2010 | Acta Biochimica et Biophysica Sinica2010，42，7： | 15 |
| 5 | Preliminary analysis of the hanging wall effect and velocity pulse of the 5.12 Wenchuan earthquake显示文摘A preliminary study of the PGA attenuation, hanging wall effect and velocity pulse characteristics from the 2008 Wenchuan earthquake of Ms =8.0 is described in this paper. The study was carried out through analyses in the time and frequency domains of main earthquake records. In the PGA attenuation study, records from 316 stations less than 1000 km from the surface rupture of the fault were used as a database and attenuation relationships were developed and compared with some existing relationships that are widely used in China's Mainland, Chinese Taiwan and the US. At the same time, records from 28 stations less than 100 km from the fault were used to study the hanging wall effect and velocity pulse characteristics of this earthquake based on the distribution of PGA, PGV, spectral acceleration, and the velocity pulse peak, and the results are compared with the 1999 Chi-Chi earthquake. In addition, the large PGAs of the UD components observed in this event are also discussed in this paper. From the results of the preliminary study, some conclusions are developed and suggestions for further research are proposed. | Liu Qifang and Li Xiaojun Institute of Engineering Mechanics, China Earthquake Administration, Harbin 150080, China Associate Professor Professor | 2009 | Earthquake Engineering and Engineering Vibration2009，8，2： | 11 |
| 6 | Hypoxia Induces Transforming Growth Factor-β1 Gene Expression in the Pulmonary Artery of Rats via Hypoxia-inducible Factor-1α显示文摘现在的学习被承担调查组织缺氧的动态表示可诱导的 factor-1a (HTF-1a ) 和在老鼠的 hypoxia-inducedpulmonary 高血压的转变生长 factor-beta1 (TGF-beta1 ) 。意味着在组织缺氧的 7 d 以后显著地增加的肺的动脉的压力(mPAP ) ,这被发现。肺的动脉改变索引和恰好室的肥大在 hypoxia.HIF-1 高山的 14 d 以后变得明显哈染色的 mRNA 在控制,为 3 d 的组织缺氧和为 7 d 的组织缺氧是不太积极的,但是开始在组织缺氧的 14 d 以后显著地提高了,然后仍然是的马厩。在控制的 HIF-1alpha 蛋白质的表示是不太积极的,但是在肺的动脉的大桶集成通信适配器是起来调整的在肺的动脉的墙中的所有 hypoxic rats.TGF-beta1 mRNA 表达式的 intima 在组织缺氧的 14 d 以后显著地被增加,但是没在组织缺氧的 3 或 7 d 以后显示出明显的变化。在肺的大桶集成通信适配器外膜和大桶集成通信适配器媒介, TGF-beta1 蛋白质染色在控制老鼠是不太积极的,但是显著地在组织缺氧的 3 d 以后被提高,到达它在组织缺氧的 7 d 以后的山峰,然后在组织缺氧的 14 和 21 d 以后变弱。西方的弄污显示出层次在组织缺氧的 7 d 以后显著地增加了然后在高 level.TGF-beta1 蛋白质水平留下了的那 HIF-1alpha 蛋白质,到达它是显著地在 3 以后提高了组织缺氧的 d 在组织缺氧的 7 d 以后说,然后在组织缺氧的 14 和 21 d 以后减少。线性关联分析显示出那 HIF-1alpha mRNA , TGF-beta1 mRNA , TGF-beta1 蛋白质断然与 mPAP ,容器 morphometry 和权利被相关室的肥大 index.TGF-beta1 蛋白质( tunicaadventitia )否定地一起与 HIF-1alpha mRNA.Taken 被相关,我们的结果建议在在组织缺氧以后的 HIF-1alpha 和 TGF-beta1 表示的变化起一个重要作用老鼠的 导致inhypoxia 的肺的高血压。 | Yongliang JIANG Aiguo DAI Qifang LI and Ruicheng HU Depoptment of Respiratory Medicine,Hunan Institute of Gerontology,Hunan Province Geriatric Hospital,Changsha 410001,China | 2007 | Acta Biochimica et Biophysica Sinica2007，39，1： | 10 |
| 7 | NON-INVASIVE IMAGING OF CORONARY ARTERY WITH 16-SLICE SPIRAL COMPUTED TOMOGRAPHY显示文摘To evaluate the value of 16-slice spiral CT in the demonstration of coronary artery and in the diagnose of coronary artery stenosis. Methods Plain and enhanced CT scans were performed with a 16-slice CT scanner (Sensation 16, Siemens, Germany) in 230 patients with suspected coronary heart disease (CHD). Parameters of the plain scan were: 120 kV, 133 mA, slice col-limation 16 mm×1.5 mm, rotation time 0.42 seconds, increment 1.5 mm, and slice width 3 mm. Parameters of the enhanced scan were: 120 kV, 500 mA, slice collimation 16 mm×0.75 mm, rotation time 0.42 seconds, increment 0.5 mm, and slice width 1 mm. Enhanced CT scan was performed with a rapid intravenous injection of 100 mL iothalamate meglumine (Ultravist) (370 mgI/mL) or Omnipaque (350 mgI/mL) and 30 mL 0.9% NaCl chaser bolus at a flow rate of 3.5 mL/s. Calcium scoring with plain scan images and two and three dimensional reconstruction with enhanced scan images were made in all cases, among which 30 cases underwent conventional coronary angiography. Demonstration of coronary arteries and their stenosis were evaluated and the factors that might influence the image quality were analyzed. Results Coronary calcium scores were calculated and coronary artery was demonstrated in our study. In the evaluationof image quality with volume rendering technique (VRT) images, 78.3% of the images were of the first class, 12.2% the sec-ond class, and 9.6% the third class. Multi-planar reconstruction (MPR) and maximal intensity projection (MIP) were better than VRT in the demonstration of small branches. The image quality was related to the heart rate, with or without arrhythmia, and breath-hold ability of patients. Comparative study of the stenosis of coronary arteries in 30 cases showed that the sensi-tivity and specificity of 16-slice coronary CT angiography (CTA) to diagnose significant stenosis were 95.8% and 94.8% resp-ectively. Conclusion As a non-invasive and quick method, 16-slice coronary CTA is sensitive and specific to diagnose the stenosis of coronary arteries and can be used as a screening method in the diagnosis of CHD. | Zhu-huaZhang Zheng-yuJin Dong-jingLi Song-baiLin Shu-yangZhang Ling-yanKong YunWang Lin-huiWang Wen-minZhao Wen-binMou Li-RenZhang Wen-lingZhu ChaoNi HuaRen Hong-quanYu QiMiao QiFang | 2004 | Chinese Medical Sciences Journal2004，19，3： | 6 |
| 8 | Three-dimensional simulations of strong ground motion in the Shidian basin based upon the spectral-element method显示文摘The strong motion of a small long and narrow basin caused by a moderate scenario earthquake is simulated by using the spectral-element method and the parallel computing technique.A total of five different geometrical profiles within the basin are used to analyze the generation and propagation of surface waves and their relation to the basin structures in both the time and frequency domain.The amplification effects are analyzed by the distribution of peak ground velocity(PGV)and cumulative kinetic energy(Ek) in the basin.The results show that in the 3D basin,the excitation of the fundamental and higher surface wave modes are similar to that of the 2D model.Small bowls in the basin have great influence on the amplification and distribution of strong ground motion,due to their lateral resonances when the wavelengths of the lateral surface waves are comparable to the size of the bowls.Obvious basin edge effects can be seen at the basin edge closer to the source for constructive interference between direct body waves and the basin-induced surface waves.The Ek distribution maps show very large values in small bowls and some corners in the basin due to the interference of waves propagating in different directions.A high impedance contrast model can excite more surface wave modes,resulting in longer shaking durations as well as more complex seismograms and PGV and Ek distributions. | Liu Qifang Yu Yanyan Zhang Xubin | 2015 | Earthquake Engineering and Engineering Vibration2015，14，3： | 6 |
| 9 | Role of N4-acetylcytidine for continuously activating NLRP3 inflammosome by HMGB1 pathway in microglia显示文摘N4-acetylcytidine(N4A) is an organic compound and a metabolite of transferrable ribonucleic acid.Its molecular formula is C_(11)H_(15)N_3O_6. | Hua Bai Qifang Zhang | 2021 | Neural Regeneration Research2021，16，7： | 4 |
| 10 | Centralized Bi-level Spatial-Temporal Coordination Charging Strategy for Area Electric Vehicles显示文摘Increased penetration of electric vehicles(EVs)is expected to impact power system performance in adverse ways,e.g.,overloading,uncertain power quality,and increased voltage fluctuation,particularly at the distribution level.Most EV charging control strategies that have been proposed only benefit the grid or EV users.A centralized EV charging strategy based on bilevel optimization is proposed in this paper with the objectives of deriving benefits for the grid and EV users simultaneously.The proposed strategy involves distributing the EV charging load more beneficially across both spatial and temporal levels.In the spatial problem,the whole fleet of EVs is controlled to minimize load variance as spatial coordination,with total charging rate and energy needed as the constraint.While in the temporal problem,EVs in each aggregator are controlled to minimize the charging cost or maximize the EV user’s degree of satisfaction with each aggregator’s charging rate and energy needed as the constraint.The proposed bi-level charging strategy is transformed to a single-stage optimization problem and solved using the classical optimization method.The impacts of uncontrolled charging on the grid and EV users are studied using the Monte Carlo Simulation(MCS)method.Then,the effectiveness of the proposed charging strategy is demonstrated via results obtained in the MCS. | Lei Yu Tianyang Zhao Qifang Chen Jianhua Zhang | 2015 | CSEE Journal of Power and Energy Systems2015，1，4： | 4 |
| 11 | Up-regulation of heme oxygenase-1 by isoflurane preconditioning during tolerance against neuronal injury induced by oxygen glucose deprivation显示文摘Heme oxygenase (惊讶) 是在降级的限制率的酶他我生产胆汁颜料和碳一氧化物。HO-1 isozyme 被象热那样的许多因素导致,他我,局部缺血,和氢过氧化物。在最近的年里,装调查结果建议了那 HO-1 举办一项 neuroprotective 活动对是化学家损害。isoflurane 的 neuroprotective 角色,一通常使用麻药,很好被记录了,但是很少对包含的内在的机制被知道。最近, isoflurane 在肝被显示出到起来调整 HO-1。在这研究,我们证明 isoflurane preconditioning 支持幸存有教养是化学家由增加熬过神经原和他们的生存能力的数字的海马趾的神经原。由反向的抄写聚合酶链反应和西方的污点分析的进一步的学习证明 isoflurane preconditioning 显著地在导致的氧葡萄糖剥夺(OGD ) 增加 HO-1 表示 neuronal 损害。而且,抑制惊讶由听 protoporphyrin 的活动部分废除 isoflurane preconditioning 测量了由的保护的效果喂奶在 OGD 神经原的脱氢酶版本。这些调查结果显示对导致 OGD 的损害的 isoflurane preconditioning 的 neuroprotective 角色可能在起来调整的 HO-1 与它的角色被联系在是化学家神经原。 | Qifang Li Yesen Zhu Hong Jiang Hui Xu Heping Liu | 2008 | Acta Biochimica et Biophysica Sinica2008，40，9： | 3 |
| 12 | A detachable coating of cholesterol-anchored PEG improves tumor targeting of cell-penetrating peptide-modified liposomes显示文摘Cell-penetrating peptides(CPPs)have been widely used to enhance the membrane transloca-tion of various carriers for many years,but the non-specificity of CPPs seriously limits their utility in vivo.In this study,cholesterol-anchored,reduction-sensitive PEG(first synthesized by our laboratory)was applied to develop a co-modified liposome with improved tumor targeting.Following optimization of the formulation,the in vitro and in vivo properties of the co-modified liposome were evaluated.The co-modified liposome had a much lower cellular uptake and tumor spheroid uptake,but a much higher tumor accumulation compared to CPP-modified liposome,indicating the non-specific penetration of CPPs could be attenuated by the outer PEG coating.With the addition of exogenous reducing agent,both the in vitro and in vivo cellular uptake was markedly increased,demonstrating that the reduction-sensitive PEG coating achieved a controllable detachment from the surface of liposomes and did not affect the penetrating abilities of CPPs.The present results demonstrate that the combination of cholestervsitive PEG and CPPs is an ideal alternative for the application of CPP-modified carriers in vivo. | Jie Tang Li Zhang Han Fu Qifang Kuang Huile Gao Zhirong Zhang Qin Hen | 2014 | Acta Pharmaceutica Sinica B2014，4，1： | 3 |
| 13 | Ameliorating the interfacial issues of all-solid-state lithium metal batteries by constructing polymer/inorganic composite electrolyte显示文摘Lithium metal is one of the most promising anodes for next-generation batteries due to its high capacity and low reduction potential.However,the notorious Li dendrites can cause the short life span and safety issues,hindering the extensive application of lithium batteries.Herein,Li_(7)La_(3)Zr_(2)O_(12)(LLZO)ceramics are integrated into polyethylene oxide(PEO)to construct a facile polymer/inorganic composite solid-state electrolyte(CSSE)to inhibit the growth of Li dendrites and widen the electrochemical stability window.Given the feasibility of our strategy,the designed PEO-LLZO-LiTFSI composite solid-state electrolyte(PLLCSSE)exhibits an outstanding cycling property of 134.2 mAh g^(-1) after 500 cycles and the Coulombic efficiency of 99.1%after 1000 cycles at 1 C in LiFePO_(4)-Li cell.When cooperated with LiNi_(0.6)Co_(0.2)Mn_(0.2)O_(2)(NCM622)cathode,the PLL-CSSE renders a capacity retention of 82.4%after 200 cycles at 0.2 C.More importantly,the uniform dispersion of LLZO in PEO matrix is tentative tested via Raman and FT-IR spectra and should be responsible for the improved electrochemical performance.The same conclusion can be drawn from the interface investigation after cycling.This work presents an intriguing solid-state electrolyte with high electrochemical performance,which will boost the development of all-solid-state lithium batteries with high energy density. | Su Wang Qifang Sun Wenxiu Peng Yue Ma Ying Zhou Dawei Song Hongzhou Zhang Xixi Shi Chunliang Li Lianqi Zhang | 2021 | Journal of Energy Chemistry2021，30，7： | 2 |
| 14 | Two-photon Excited Fluorescence of Bithiophene Derivatives显示文摘 | GuangMingXIA QiFANG XinGuangXU GuiBaoXU WeiWANG ZhiQiangLIU GuiLanSONG YongJieWU | 2003 | Chinese Chemical Letters2003，14，6： | 2 |
| 15 | An MPC-based manoeuvre stability controller for full drive-by-wire vehicles显示文摘Aiming at the actuator time delay caused by the drive-by-wire technology,a novel manoeuvre stability controller based on model predictive control is proposed for full drive-by-wire vehicles.Firstly,the future vehicle dynamics are predicted by a twodegree-of-freedom vehicle model with input delay.Secondly,in order to prevent the vehicle from destabilizing due to excessive side slip angles,the determined ideal yaw rate and side slip angle are tracked simultaneously by optimizing the front wheel angle and additional yaw moment.Moreover,in order to improve the trajectory tracking ability,a side slip angle constraint determined by phase plane stability boundaries is added to the cost function.The results of Matlab and veDYNA co-simulation show that the regulated yaw rate can track the reference value well and the side slip angle decreases.Meanwhile,the trajectory tracking ability is improved obviously by compensating the time delay. | Ping WANG Ziyang LIU Qifang LIU Hong CHEN | 2019 | Control Theory and Technology2019，17，4： | 2 |
| 16 | Controllable synthe- sis of Bi2WO6 nanofibrous mat by electrospinning and enhanced visible photocatalytic degradation performances 显示文摘 | Zhao Gang Liu Suwen Lu Qifang | 2012 | Industrial & Engineering Chemistry Research2012，51，10： | 1 |
| 17 | 显示文摘 | Song Lingjun Liu Suwen Lu Qifang | 2012 | Applied Surface Science2012，258，8： | 1 |
| 18 | Compatibility and thermal properties of poly ( acrylonitrile-butadiene-styrene ) copolymer blends with poly (methylmethacrylate) and poly ( styrene-coacrylo- nitrile) 显示文摘 | LI Qifang TIAN Ming | 2002 | J Appl Polym Sei2002，85，： | 1 |
| 19 | The synthesis and characterization of polystyrene/magnetic polyhedral oligomeric silsesquioxane (POSS) nanocomposites 显示文摘 | Song Xiaoyan Geng Haiping Li Qifang | 2006 | Polymer2006，47，9： | 1 |
| 20 | Dynamic analysis of a pilot-oper- ated two-stage solenoid valve used in pneumatic system显示文摘 | Ye Qifang Chen Jiangping | 2009 | Simulation Modelling Practice and Theory2009，17，5： | 1 |
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