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| 1 | Characterization and enrichment of hepatic progenitor cells in adult rat liver显示文摘AIM: To detect the markers of oval cells in adult rat liver and to enrich them for further analysis of characterization in vitro.METHODS: Rat model for hepatic oval cell proliferation was established with 2-acetylaminofluorene and two third partial hepatectomy (2-AAF/PH). Paraffin embedded rat liver sections from model (11 d after hepatectomy) and control groups were stained with HE and OV6, cytokeratin19 (CK19),albumin, alpha fetoprotein (AFP), connexin43, and c-kit antibodies by immunohistochemistry. Oval cell proliferation was measured with BrdU incorporation test. C-kit positive oval cells were enriched by using magnetic activated cell sorting (MACS) .The sorted oval cells were cultured in a low density to observe colony formation and to examine their characterization in vitroby immunocytochemistry and RT-PCR.RESULTS: A 2-AAF/PH model was successfully established to activate the oval cell compartment in rat liver. BrdU incorporation test of oval cell was positive. The hepatic oval cells coexpressed oval cell specific marker OV6, hepatocytemarker albumin and cholangiocyte-marker CK19. They also expressed AFP and connexin 43. C-kit, one hematopoietic stem cell receptor, was expressed in hepatic oval cells at high levels. By using c-kit antibody in conjunction with MACS,we developed a rapid oval cell isolation protocol. The sorted cells formed colony when cultured in vitro. Cells in the colony expressed albumin or CK19 or coexpressed both and BrdU incorporation test was positive. RT-PCR on colony showed expression of albumin and CK19 gene.CONCLUSION: Hepatic oval cells in the 2-AAF/PH model had the properties of hepatic stem/progenitor cells. Using MACS, we established a method to isolate oval cells. The sorted hepatic oval cells can form colony in vitro which expresses different combinations of phenotypic markers and genes from both hepatocytes and cholangiocyte lineage. | Ai-LanQin Xia-QiuZhou WeiZhang HongYu Qinxie | 2004 | World Journal of Gastroenterology2004，10，10： | 18 |
| 2 | Axin bridges Daxx to p53显示文摘死亡联系域的蛋白质 Daxx 施加包括调停的许多报导功能经由激活 c6 月 N 终端从 FasL 发信号到 apoptosis apoptosis 的 kinase (JNK ) ,正式就职和抑制,和染色质改变的规定。它原来从酵母被克隆用船边交货对相似物体之连续感觉而形成心像口的细胞内部的尾巴的二混血儿的屏幕诱饵。而许多起始的报告仍然保持争论, Daxx 在一系列压力信号包括 UVirradiation,过氧化氢治疗和 TGF- β治疗触发的 apoptosis 的规定起重要作用,是清楚的。在这评论,我们在 Axinbeing 上集中连接 Daxx 到 p53 的一个拴住的因素。 | Sheng-Cai Lin Qinxi Li | 2007 | Cell Research2007，17，4： | 5 |
| 3 | APP physiological and pathophysiological functions: insights from animal models显示文摘淀粉的先锋蛋白质(应用软件) 在最近的年里的集中的学习下面,主要由于它在 Alzheimer 的疾病(广告) 的致病的关键角色。β淀粉(Aβ) 从应用软件解朊的劈开产生的肽能聚集,在人的广告大脑导致匾形成。削尖影响 Aβ 的应用软件的变化;生产被发现为世袭早发作原因家庭广告。它是很可能的那阐明应用软件的生理的性质将极大地在广告致病便于它的角色的理解。很多个应用软件 loss-of-function 和 gain-of-function 模型包括 Caenorhabditis elegans,果蝇, zebrafish 和老鼠在模型有机体被建立了。这些在 vivo 模型提供我们珍贵卓见进应用软件生理的函数。另外,几只敲门在里面老鼠当模特儿在生理的水平表示变异的应用软件是可得到的允许我们没有应用软件 overexpression,学习广告致病。这篇文章将考察生理的水流和应用软件的 pathophysiological 动物模型。 | Qinxi Guo Zilai Wang Hongmei Li Mary Wiese Hui Zheng | 2012 | Cell Research2012，22，1： | 5 |
| 4 | The Axin/TNKS complex interacts with KIF3A and is required for insulin-stimulated GLUT4 translocation显示文摘由葡萄糖 transporter GLUT4 的刺激胰岛素的葡萄糖举起在整个身体的葡萄糖动态平衡起一个中央作用,哪个导致类型 2 糖尿病的 dysregulation。然而,调整刺激胰岛素的葡萄糖举起的分子的部件和机制仍然保持大部分不清楚。这里,我们证明 Axin 与 ADP-ribosylase tankyrase 交往 2 (TNKS2 ) 并且 kinesin 马达蛋白质 KIF3A,响应胰岛素形成为 GLUT4 translocation 关键的第三的建筑群。特定到血浆膜的复杂稀释刺激胰岛素的 GLUT4 translocation 的单个部件击倒。重要地, TNKS2 −/− 老鼠展出减少的胰岛素敏感和更高的血葡萄糖层次什么时候在 fasting 以后的 re-fed。机械学地,我们证明当胰岛素不在时, Axin, TNKS 和 KIF3A 与 trans-Golgi 网络上的 GLUT4 是 co 局部性的。胰岛素处理压制 TNKS 的 ADP-ribosylase 活动,导致在自动数据处理 ribosylation 和 ubiquitination 的减小 Axin 和 TNKS,和建筑群的并发的稳定。Akt 的抑制,胰岛素发信号的主要受动器 kinase,废除调停胰岛素的复杂稳定。我们因此阐明了直接在刺激胰岛素的 GLUT4 translocation 与马达蛋白质 kinesin 被联系的新蛋白质建筑群。 | Hui-Ling Guo Cixiong Zhang Qi Liu Qinxi Li Guili Lian Di Wu Xuebin Li Wei Zhang Yuemao Shen Zhiyun Ye Slau-Yong Lin Sheng-Cai Lin | 2012 | Cell Research2012，22，8： | 4 |
| 5 | REINORCING THEORY AND TECHNIQUE OF SLOPE EXCAVATION显示文摘Afteraseriesoflargescalemodellingtest,theoreticalanalysesanddigitalmodellinganalysesofslopeexcava-tionincompositesoilbeingreinforcedwithsteelbars,thepropertyandbearingcapacityofcompositesoilslopewereanalyzedsystematically.Thereinforcingtheoryforslopeexcavationwassoputforward.thatis,activeretainingsta-bilitymechanism,andreinforcingtechniqueforexcavationslopewithsteelbars. | Huang Yutian, Zhang Qinxi, Gao Huadong(Beijing Polytechnic University,Beijing 100022 ) | 1996 | 西部探矿工程1996，8，5： | 1 |
| 6 | Existence and stability of periodic solutions for Hopfield neutral network equations with periodic input显示文摘 | Dong Qinxi Matsui K Huang Xiankai | 2002 | Nonlinear Analysis TMA2002，49，4： | 1 |
| 7 | Potential protection of green tea polyphenols against intracel ular amyloid beta-induced toxicity on primary cultured prefrontal cortical neurons of rats显示文摘 | QinXY Cheng Y Yu LC | | 0，，02： | 1 |
| 8 | Advancesofresearchontobaccobushytopdisease(inChinese)显示文摘 | ChenHR LiF QinXY etal | 2006 | 烟草农业科学2006，2，1： | 1 |
| 9 | Glial‐derived neurotrophic factor regulates intestinal epithelial barrier function and inflammation and is therapeutic for murine colitis显示文摘 | Dei KuiZhang Fu QianHe Tian KeLi Xue HuaPang De JunCui QinXie Xiao LiHuang Hua TianGan | 2010 | J Pathol2010，，2： | 1 |
| 10 | 显示文摘 | 秦雪英(QinXY) 汪靖伦(WangJL) 张灵志(ZhangLZ) | 2012 | 化学进展2012，24，5： | 1 |
| 11 | Advances of research on tobacco bushy top disease显示文摘 | ChenHR LiF QinXY etal | 2006 | 烟草农业科学2006，2，1： | 1 |
| 12 | Analysis of monitoring and numerical simulation on anchor force of foundation pit显示文摘 | WANG Guihe ZHANG Qinxi HUANG Xuegang | 2013 | Przeglad Elektrotechniczny2013，89，3： | 1 |
| 13 | HSPBAP1 is found extensively in the anterior temporal neocortex of patients with intractable epilepsy显示文摘 | Zhi‐QinXi Ji‐JunSun Xue‐FengWang Ming‐WeiLi Xian‐ZengLiu Lin‐YuanWang XiZhu FeiXiao Jin‐MeiLi YunGong Li‐FengGuan | 2007 | Synapse2007，，9： | 1 |
| 14 | VERIFICATION OF REINFORCING TECHNIQUE OF SLOPE EXCAVATION WITH显示文摘hetechniqueofslopeexcavationwithreinforcedsteelbarswasdevelopedbasedonthemechanismofactivere-tainingstability。Alotofengineeringpracticesexperiencesshowedthatthisreinforcingtheoryisverifiedanditstech-niqueissuccessful。Soatheoreticalbasisforthedevelopmentofthistechniquewaspresented。 | Huang Yutian,Zhang Qinxi (ijing Polytechnic UniversityBeijing,100022 ) | 1996 | 西部探矿工程1996，8，5： | 0 |
| 15 | TEST IN-SITU AND STUDIES ON REINFORCING SLOPE INSERTING WITH STE显示文摘Thedesign,conditionandtechnicalrneasureswereintroducedoftestin-situofreinforcingslopeinsertingwithsteelbars.Acertainregularitieshavebeenacquiredfromthetest,examinationandstudy.Theresultswerealsoanal-ysed. | Huang Yutian Zhang Qinxi Gao Huadong(Beijing Polytechnical University,Beijing 100022 ) | 1996 | 西部探矿工程1996，8，5： | 0 |
| 16 | COHESION PROPERTY BETWEEN GROUTING MIXTURE AND INSERTING BA显示文摘Researchandmeasurementofthecohesionbetweenseveralnormally-usedgroutingmaterialsandconcretehavebeendone,andrelationbetweenthecohesionandmaterialspropertyaimedatfindingoutaneconomicandefficientgroutingmaterialssoastouselesscementandcutthecosthasbeeninvestigated. | Huang Yutian Zhang Qinxi Zhao Guoqing(Beijing Polytechnic University,Beijing 100022 ) | 1996 | 西部探矿工程1996，8，5： | 0 |
| 17 | High sensitivity of semimetal photodetection via Bose–Einstein condensation显示文摘The discovery of semiconductor has witnessed remarkable strides toward high performance of photodetectors attributed to its excellent carrier properties.However,semimetal,owning to the high carrier concentration and low carrier mobility compared to those of semiconductor,is generally considered unsuitable for photodetection.Herein,we demonstrate an outstanding photodetection in a layered semimetal titanium diselenide(TiSe_(2))in Bose-Einstein condensation(BEC)state.High sensitivity of semimetal photodetector is realized in the range of visible,infrared and terahertz bands.The noise equivalent power(NEP)has threefold improvement at the visible and infrared wavebands,and significant decrease by one order of magnitude in the terahertz frequencies via BEC phenomenon,attributed to the electrical parameter variation after condensation.The best NEP value in the terahertz frequency is comparable to that of commercial Si photodetector.Our results show another recipe to fabricate high performance of photodetection via semimetal except for semiconductor and pave the way to exploit macroscopic quantum phenomena for optoelectronics. | Tuntan Wu Qinxi Qiu Yongzhen Li Qiangguo Zhou Wanli Ma Jingbo Li Lin Jiang Wei Zhou Zhiming Huang | 2024 | InfoMat2024，6，2： | 0 |
| 18 | Earthquake Monitoring and Study in the Jingpohu Volcano Cluster Area显示文摘Seismicity in the Jingpohu volcanic area was investigated based on the seismic data recorded by the mobile seismic network consisting of 14 stations equipped with 24-bit broad-band 3-component seismographs around Crater Forest.Results show that there appears certain seismicity in Jingpohu and its adjacent areas with a low activity level and most of the recorded earthquakes are the volcanic-tectonic ones.The results of location indicate a dominant focal depth of 10km~30km,most of the earthquakes are smaller than M_L2.0,and are concentrated in the area of 'Crater Forest' and on the Dunhua-Mishan fault which runs through the volcanic area.At station No.2,which has better observation conditions,two types of events,likely associated to volcanism,were recorded;their waveform characteristics are somewhat similar to that of the long-period volcanic event and the volcanic tremor,but with different feature of frequencies. | Liu Zhi Duan Yonghong Xu Zhaofan Yuan Qinxi Yang Jian Zhou Xuesong | 2008 | Earthquake Research in China2008，22，2： | 0 |
| 19 | A new method for preparing a rat intracerebral hemorrhage model by combining focused ultrasound and microbubbles显示文摘Background:We aimed to prepare a non-invasive,reproducible,and controllable rat model of intracerebral hemorrhage with focused ultrasound(FUS).Methods:A rat intracerebral hemorrhage(ICH)model was established by combining FUS and microbubbles(μBs),and edaravone was used to verify whether the free radical scavenger had a protective effect on the model.The brain tissue of each group was sectioned to observe the gross histology,blood-brain barrier(BBB)permeability,cerebral infarction volume,and histopathological changes.Results:Compared with the FUS group,the BBB permeability was significantly increased in the FUS+μBs(F&B)group(p=0.0021).The second coronal slice in the F&B group had an obvious hemorrhage lesion,and the FUS+μBs+edaravone(F&B&E)group had smaller hemorrhage areas;however,ICH did not occur in the FUS group.The cerebral infarction volume in the F&B group was significantly larger than that in the FUS group(p=0.0030)and F&B&E group(p=0.0208).HE staining results showed that nerve fibrinolysis,neuronal necrosis,microglia production,and erythrocytes were found in both the F&B group and the F&B&E group,but the areas of the nerve fibrinolysis and neuronal necrosis in the F&B group were larger than the F&B&E group.Conclusions:A rat ICH model was successfully prepared using theμBs assisted FUS treatment,and edaravone had a therapeutic effect on this model.This model can be used to study the pathophysiological mechanism of ICH-related diseases and in preclinical research on related new drugs. | Yao He Jie Yang Fengying Hu Min Liao Yuru Nie Xiaoxia Zhu Tao Zhang Keer Song Qinxi Li Xiaojie Li Chenghan Mei Zhe Wu Qiang Lu Zhihui Zhong | 2023 | Animal Models and Experimental Medicine2023，6，2： | 0 |
| 20 | Analysis of pressure response at an observation well against pressure build-up by early stage of CO_(2)geological storage project显示文摘To ensure a safe and stable CO_(2)storage,pressure responses at an observation well are expected to be an important and useful field monitoring item to estimate the CO_(2)storage behaviors and the aquifer parameters during and after injecting CO_(2),because it can detect whether the injected CO_(2)leaks to the ground surface or the bottom of the sea.In this study,pressure responses were simulated to present design factors such as well location and pressure transmitter of the observation well.Numerical simulations on the pressure response and the time-delay from pressure build-up after CO_(2)injection were conducted by considering aquifer parameters and distance from the CO_(2)injection well to an observation well.The measurement resolution of a pressure transmitter installed in the observation well was presented based on numerical simulation results of the pressure response against pressure build-up at the injection well and CO_(2)plume front propagations.Furthermore,the pressure response at an observation well was estimated by comparing the numerical simulation results with the curve of CO_(2)saturation and relative permeability.It was also suggested that the analytical solution can be used for the analysis of the pressure response tendency using pressure build-up and dimensionless parameters of hydraulic diffusivity.Thus,a criterion was established for selecting a pressure transducer installed at an observation well to monitor the pressure responses with sufficient accuracy and resolution,considering the distance from the injection well and the pressure build-up at the injection well,for future carbon capture and storage(CCS)projects. | Qiang Sun Kyuro Sasaki Qinxi Dong Zhenni Ye Hui Wang Huan Sun | 2024 | Journal of Rock Mechanics and Geotechnical Engineering2024，16，2： | 0 |
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