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| 1 | Predominant mucosal expression of 5-HT_(4(+h)) receptor splice variants in pig stomach and colon显示文摘AIM: To investigate cellular 5-HT4(-h/+h) receptor distribution, particularly in the epithelial layer, by laser mi-crodissection and polymerase chain reaction (PCR) in porcine gastrointestinal (GI) tissues. METHODS: A stepwise approach was used to evaluate RNA quality and to study cell-specific 5-HT4 receptor mRNA expression in the porcine gastric fundus and colon descendens. After freezing, staining and laser microdissection and pressure catapulting (LMPC), RNA quality was evaluated by the Experion automated electrophoresis system. 5-HT4 receptor and glyceral-dehyde-3-phosphate dehydrogenase (GAPDH) expressions were examined by endpoint reverse transcription (RT)-PCR in mucosal and muscle-myenteric plexus (MMP) tissue fractions, in mucosal and MMP parts of hematoxylin and eosin (HE) stained tissue sections andin microdissected patches of the epithelial and circular smooth muscle cell layer in these sections. Pig gastric fundus tissue sections were also stained immunohisto-chemically (IHC) for enterochromaffin cells (EC cells; MAB352); these cells were isolated by LMPC and examined by endpoint RT-PCR. RESULTS: After HE staining, the epithelial and circular smooth muscle cell layer of pig colon descendens and the epithelial cell layer of gastric fundus were identified morphologically and isolated by LMPC. EC cells of pig gastric fundus were successfully stained by IHC and isolated by LMPC. Freezing, HE and IHC staining, and LMPC had no influence on RNA quality. 5-HT4 recep-tor and GAPDH mRNA expressions were detected in mucosa and MMP tissue fractions, and in mucosal and MMP parts of HE stained tissue sections of pig colon descendens and gastric fundus. In the mucosa tissue fractions of both GI regions, the expression of h-exon containing receptor [5-HT4(+h) receptor] mRNA was significantly higher (P<0.01) compared to 5-HT4(-h) re-ceptor expression, and a similar trend was obtained in the mucosal part of HE stained tissue sections. Large microdissected patches of the epithelial and circular smooth muscle cell layer of pig colon descendens and of the epithelial cell layer of pig gastric fundus, also showed 5-HT4 receptor and GAPDH mRNA expression. No 5-HT4 receptor mRNA expression was detected in gastric LMPC-isolated EC cells from IHC stained tissues, which cells were positive for GAPDH. CONCLUSION: Porcine GI mucosa predominantly expresses 5-HT4(+h) receptor splice variants, suggesting their contribution to the 5-HT4 receptor-mediated mu-cosal effects of 5-HT. | Evelien KV Priem Joris H De Maeyer Mado Vandewoestyne Dieter Deforce Romain A Lefebvre | 2013 | World Journal of Gastroenterology2013，19，24： | 2 |
| 2 | Skeletal Muscle PGC-1α1 Modulates Kynurenine Metabolism and Mediates Resilience to Stress-Induced Depression显示文摘 | Leandro Z. Agudelo Teresa Femenía Funda Orhan Margareta Porsmyr-Palmertz Michel Goiny Vicente Martinez-Redondo Jorge C. Correia Manizheh Izadi Maria Bhat Ina Schuppe-Koistinen Amanda T. Pettersson Duarte M.S. Ferreira Anna Krook Romain Barres Juleen R. Zi | 2014 | Cell2014，，1： | 1 |
| 3 | Global strategy for the diagnosis,management,and prevention of chronic obstructive pulmonary disease显示文摘 | ROMAIN A PAUWELS A SONIA B | 2001 | Am J Respir Crit Care Med2001，163，5： | 1 |
| 4 | Thymidine kinase as a proliferative marker: clinical relevance in 1692 primary breast cancer patients 显示文摘 | Brot P Romain S Daver A | 2001 | J Clin Oncol2001，19，： | 1 |
| 5 | Thymidine kinase as a prolifer- ative marker: clinical relevance in 1692 primary breast cancer patients显示文摘 | Brot P Romain S Daver A | 2001 | J Clin Oneol2001，19，11： | 1 |
| 6 | Thymidine Kinase as a proliferative marker:clinical relevance in 1,692 primary bresast cancer patients显示文摘 | Broet P Romain S Daver A | | 0，，11： | 1 |
| 7 | Long term stability of metal oxide- based gas sensors for e-nose environmental applications: an overview显示文摘 | Romain A C Nicolas J | 2010 | Sensors and Actuators B: Chemical2010，146，2： | 1 |
| 8 | Effect of late percutaneous angioplastic recanalization of total coronary artery occlusion on left ventricular remodeling,EF and RWM显示文摘 | Nicolas D Michael A Romain C | 1996 | Am J Cardiol1996，78，： | 1 |
| 9 | The electronic nose as a warning device of the odour emergence in a compost hall显示文摘 | Nicolas J Romain A Ledent C | | 0，，： | 1 |
| 10 | Long term stability of metal oxide-based gas sensors for e-nose environmental applications:an overview显示文摘 | Romain A C Nicolas J | 2010 | Sensors and Actuators B: Chemical2010，146，2： | 1 |
| 11 | Thymidine kinase as a proliferative marder:clinical relevance in 1 692 primary breast caner patients显示文摘 | Bro(e)t P Romain S Daver A | 2001 | J Clin Oncol2001，19，11： | 1 |
| 12 | Asymptotic theory for the principal component analysis of a vector random function:Some applications to statistical inference显示文摘 | J Dauxois A Pousse Y Romain | 1982 | Journal of Multivariate Analysis1982，12，1： | 1 |
| 13 | Thymidine kinase as a proliferative marker: clinical relevance in 1,692 primary breast cancer patients 显示文摘 | Broet P Romain S Daver A | 2001 | J Clin Oncol2001，19，11： | 1 |
| 14 | A New Motion Parameter Estimation Algorithm Based on The Continuous Wavelet Transform显示文摘 | Femando A Mujica Jean-Pierre Leduc Romain Murenzi | 2000 | IEEE Transactions on Image Processing2000，9，5： | 1 |
| 15 | Long term stability of metal oxide-based gas sensors for e-nose environmental applica- tions: An overview显示文摘 | ROMAIN A C NICOLAS J | 2010 | Sensors and Actuators B2010，146，2： | 1 |
| 16 | Treatment of ox-aliplatin-induced peripheral neuropathy by intravenous mangafo-dipir显示文摘 | ROMAIN C JEROME A CAROLE N | 2014 | J Clin Invest2014，124，1： | 1 |
| 17 | Detection of diverse mould speeies growing on building materials by gas sensor arrays and pattern recognition显示文摘 | KUSKE M PADILLA M ROMAIN A C | 2006 | Sensors and Actuators B:Chemical2006，119，1： | 1 |
| 18 | Thymidine kinase as a proliferative marker: clinical relevance in 1,692 primary breast cancer patients 显示文摘 | BROET P ROMAIN S DAVER A | 2001 | J Clin Oncol2001，19，11： | 1 |
| 19 | Long Term Stability of Metal Oxide-Based Gas Sensors for E-Nose Environmental Applications:An Overview显示文摘 | Romain A Nicolas J | | 0，，2： | 1 |
| 20 | Hypericin and photodynamic therapy decreases human pancreatic cancer in vitro and in vivo显示文摘 | Carson D L Kwan D Romaine A | 2000 | J Surg Res2000，93，1： | 1 |
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