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| 1 | Loss of disabled-2 expression is an early event in esophageal squamous tumorigenesis显示文摘AIM: Disabled-2 (DAB2) is a candidate tumor-suppressor gene identified in ovarian cancer that negatively influences mitogenic signal transduction of growth factors and blocks ras activity. In a recent study, we observed down-regulation of DAB2 transcripts in ESCCs using cDNA microarrays. In the present study, we aimed to determine the clinical significance of loss of DAB2 protein in esophageal tumorigenesis, hypothesizing that DAB2 promoter hypermethylation-mediated gene silencing may account for loss of the protein. METHODS: DAB2 expression was analyzed by immunohistochemistry in 50 primary esophageal squamous cell carcinomas (ESCCs), 30 distinct hyperplasia, 15 dysplasia and 10 non-malignant esophageal tissues. To determine whether promoter hypermethylation contributes to loss of DAB2 expression in ESCCs, methylation status of DAB2 promoter was analyzed in DAB2 immuno-negative tumors using methylation-specifi c PCR. RESULTS: Loss of DAB2 protein was observed in 5/30 (17%) hyperplasia, 10/15 (67%) dysplasia and 34/50 (68%) ESCCs. Significant loss of DAB2 protein was observed from esophageal normal mucosa to hyperplasia, dysplasia and invasive cancer (Ptrend < 0.001). Promoter hypermethylation of DAB2 was observed in 2 of 10 (20%) DAB2 immuno-negative ESCCs. CONCLUSION: Loss of DAB2 protein expression occurs in early pre-neoplastic stages of development of esophageal cancer and is sustained down the tumorigenic pathway. Infrequent DAB2 promoter methylation in ESCCs suggests that epigenetic genesilencing is only one of the mechanisms causing loss of DAB2 expression in ESCCs. | Kumar Anupam Chatopadhyay Tusharkant Siddhartha Datta Gupta Ralhan Ranju | 2006 | World Journal of Gastroenterology2006，12，37： | 11 |
| 2 | siRNA-mediated downregulation of TC21 sensitizes esophageal cancer cells to cisplatin显示文摘AIM:To determine the functional significance of TC21 in esophageal squamous cell carcinoma(ESCC).METHODS:TC21 siRNA transfection was carried out using Hyperfectamine to knock down TC21,and transcripts were analyzed by reverse transcription-polymerase chain reaction and protein by Western blotting.We demonstrated the effect of TC21 downregulation of cell signaling in esophageal cancer cells by assessing the phosphorylation status of its downstream targets,phosphoinositide 3-kinase(PI3K),phosphatase and tensin homolog(PTEN),protein kinase B(pAkt),nuclear factor-B(NF-B) and cyclinD1 using specific antibodies.Cell survival analysis after cisplatin treatment was carried out by cell viability assay and cell cycle analysis using flow cytometry.RESULTS:TC21 knockdown in human ESCC cell line TE13 cells,showed only a marginal increase(14.2%) in cell death compared with control cells.The expressions of the signaling proteins PI3K and pAkt,transcription factor NF-B,and cell cycle protein cyclin D1 were markedly decreased in response to TC21 downregulation,whereas the level of pPTEN,an antagonist of PI3K,was increased.In addition,we evaluated the potential of TC21 as a putative target for sensitizing ESCC cells to the chemotherapeutic agent cisplatin.Increased cell death(38.4%) was observed in cells treated with cisplatin after TC21 knockdown compared with cells which were treated with cisplatin alone(20% cell death).CONCLUSION:Results suggest that TC21 mediates its effects via the PI3K-Akt pathway,NF-B and cyclin D1,and enhances chemoresistance in esophageal cancer cells. | Md.Raghibul Hasan Shyam Singh Chauhan Rinu Sharma Ranju Ralhan | 2012 | World Journal of Gastroenterology2012，18，31： | 3 |
| 3 | Role of SPECT/CT in differentiated thyroid cancer显示文摘 | Tara D. Barwick Ranju T. Dhawan Valerie Lewington | 2012 | Nuclear Medicine Communications2012，，8： | 2 |
| 4 | Contingent valuation of ecotourism in Annapurna conservation area, Nepal: Implications for sustainable park finance and local development显示文摘 | Nabin Baral Marc J. Stern Ranju Bhattarai | 2008 | Ecological Economics2008，，2： | 1 |
| 5 | In vitro cytotoxie, an- tioxidative and ct-glueosidase inhibitory potential of a herbal mixture comprised of Allium sativum and Lagerstroemia speciosa 显示文摘 | Kesavanarayanan KS Sathiya S Ranju V | 2012 | Eur Rev Med Pharmacol Sci2012，16，3： | 1 |
| 6 | iTRAQ-Multidimensional liquid chromatography and tandem mass spectrometry-based identification of potential biomarkers of oral epithelial dysplasia and novel networks between inflammation and premalignancy 显示文摘 | RANJU RALHAN LEROI V DESOUZA AJAY MATI'A | 2009 | Journal of Protcome Research2009，8，1： | 1 |
| 7 | Epigenetic alterations of CDH1 and APC genes: Relationship with activation of Wnt/β-catenin Pathway in invasive ductal carcinoma of breast显示文摘 | Chandra P. Prasad Sameer Mirza Gayatri Sharma Rajinder Prashad Siddhartha DattaGupta Gayatri Rath Ranju Ralhan | 2008 | Life Sciences2008，，9： | 1 |
| 8 | The effect of addition of intrathecal clonidine to hyperbaric bupivacaine on postoperative pain after lower segment caesarean section: A randomized control trial显示文摘 | Ranju Singh Deepti Gupta Aruna Jain | 2013 | Saudi Journal of Anaesthesia2013，，3： | 1 |
| 9 | Frequent loss of Dab2 protein and infrequent promoter hypermethylation in breast cancer显示文摘 | Sarangadhara Appala Raju Bagadi Chandra Prakash Prasad Anurag Srivastava Rajinder Prashad Siddarth Datta Gupta Ranju Ralhan | 2007 | Breast Cancer Research and Treatment2007，，3： | 1 |
| 10 | Changing pattern of antibiotic sensitivity of Salmonella typhi显示文摘 | Ranju C Pais P Ravindran GD | 1998 | Natl Med J Ind1998，11，： | 1 |
| 11 | Telmisartan attenuates MPTP induced dopaminergic degeneration and motor dysfunction through regulation of α-synuclein and neurotrophic factors (BDNF and GDNF) expression in C57BL/6J mice显示文摘 | Sekar Sathiya Vijayan Ranju Periyathambi Kalaivani Raju Jyothi Priya Haridass Sumathy Adoor Gopalan Sunil Chidambaram Saravana Babu | 2013 | Neuropharmacology2013，，： | 1 |
| 12 | Discovery and verification of head-and-neck cancer biomarkers by differential protein expression analysis using iTRAQ labeling, multidimensional liquid chromatography, and tandem mass spectrometry显示文摘 | Ranju R DeSouza L V Matta A | 2008 | Molecular and Cellular Proteomics2008，7，6： | 1 |
| 13 | MGMT expression in oral precancerous and cancerous lesions: Correlation with progression, nodal metastasis and poor prognosis显示文摘 | Meenakshi Sawhney Nidhi Rohatgi Jatinder Kaur Siddhartha D. Gupta Suryanaryana V.S. Deo Nootan K. Shukla Ranju Ralhan | 2006 | Oral Oncology2006，，5： | 1 |
| 14 | Total oligomeric flavonoids of Cyperus rotundus ameliorates neurological deficits, excitotoxicity and behavioral alterations induced by cerebral ischemic–reperfusion injury in rats显示文摘 | A.G. Sunil K.S. Kesavanarayanan P. Kalaivani S. Sathiya V. Ranju R. Jyothi Priya B. Pramila F.D. Solomon Paul J. Venkhatesh C. Saravana Babu | 2011 | Brain Research Bulletin2011，，： | 1 |
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