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| 1 | Characterization of focal liver lesions with SonoVue~-enhanced sonography: International multicenter-study in comparison to CT and MRI显示文摘AIM: To evaluate in a multicenter study whether the sonographic characterization of focal liver lesions can be improved using SonoVueüà-enhancement; and to compare this method with computed tomography (CT) and magnetic resonance imaging (MRI). METHODS: One hundred and thirty four patients with one focal liver lesion detected in baseline ultrasound (US) were examined with conventional US, contrastenhanced US (n = 134), contrast-enhanced CT (n = 115) and/or dynamic contrast-enhanced MRI (n = 70). The lesions were classified as malignant, benign or indeterminate and the type of lesion was determined. The f inal diagnosis based on the combined information of all imaging examinations, clinical information and histology (n = 32) was used. Comparisons were made to see whether the addition of contrast-enhanced US led to the improvement of the characterization of doubtful focal liver lesions. RESULTS: In comparison with unenhanced US, SonoVue markedly improves sensitivity and specificity for the characterization (malignant/benign) of focal liver lesions. In comparison with CT and/or dynamic MRI, SonoVueüà -enhanced sonography applied for characterization of focal liver lesions was 30.2% more sensitive in the recognition of malignancy and 16.1% more specific in the exclusion of malignancy and overall 22.9% more accurate. In the subgroup with confirmative histology available (n = 30), sensitivity was 95.5% (CEUS), 72.2% (CT) and 81.8% (MRI), and specif icity was 75.0% (CEUS), 37.5% (CT) and 42.9% (MRI). The sensitivity and specif icity of CEUS for the identif ication of focal nodular hyperplasia (FNH) and hemangiomas was 100% and 87%, resulting in an accuracy of 94.5%. CONCLUSION: SonoVueüà-enhanced sonography emerges as the most sensitive, most specif ic and thus most accurate imaging modality for the characterization of focal liver lesions. | Hervé Trillaud Jean-Michel Bruel Pierre-Jean Valette Valérie Vilgrain Gérard Schmutz Raymond Oyen Wieslaw Jakubowski Jan Danes Vlastimil Valek Christian Greis | 2009 | World Journal of Gastroenterology2009，15，30： | 34 |
| 2 | Rat model of cholelithiasis with human gallstones implanted in cholestasis-induced virtual gallbladder显示文摘AIM:To facilitate translational research on cholelithiasis,we have developed a rat model of human gallstones by exploiting the unique biliopancreatic features of this species.METHODS:Under anesthesia,16 adult rats of equal genders underwent two times of abdominal surgery.First,their common bile duct(CBD)was ligated to cause cholestasis by total biliary obstruction(TBO).On day 0,1,3,7,14,21 and 28 after TBO,magnetic resonance imaging(MRI)was conducted to monitor the dilatation of the CBD,and blood was sampled to analyze total serum bilirubin(TSB).Secondly,on day 30,the abdomen was re-opened and gallstone(s)collected from human patients were implanted in the dilated CBD asa virtual gallbladder(VGB),which was closed by suture ligation.This rat cholelithiasis model was examined by MRI,clinical observation,microcholangiography and histology.RESULTS:All rats survived two laparotomies.After ligation,the CBD was dilated to a stable size of 4 to30 mm in diameter on day 21-28,which became a VGB.The rats initially showed signs of jaundice that diminished over time,which paralleled with the evolving TSB levels from 0.6±0.3 mg/d L before ligation,through a peak of 10.9±1.9 mg/dL on day 14,until a nearly normalized value after day 28.The dilated CBD with thickened wall allowed an incision for implantation of human gallstones of 1-10 mm in diameter.The rat cholelithiasis was proven by in vivo MRI and postmortem microcholangiography and histomorphology.CONCLUSION:A rat model cholelithiasis with human gallstones has been established,which proves feasible,safe,reliable,nontoxic and cost-effective.Given the gallstones of human origin,applications of this model may be of help in translational research such as optical detection and lysis of gallstones by systemic drug administration. | Marlein Miranda Cona Yewei Liu Ting Yin Yuanbo Feng Feng Chen Stefaan Mulier Yue Li Jian Zhang Raymond Oyen Yicheng Ni | 2016 | World Journal of Methodology2016，6，2： | 7 |
| 3 | Factors influencing technical success and outcome of percutaneous balloon angioplasty in de novo native hemodialysis arteriovenous fistulas显示文摘 | Sam Heye Geert Maleux Johan Vaninbroukx Kathleen Claes Dirk Kuypers Raymond Oyen | 2011 | European Journal of Radiology2011，，9： | 1 |
| 4 | Differential diagnosis of gallstones by using hypericin as a fluorescent optical imaging agent显示文摘AIM: To explore the feasibility of using hypericin as an optical imaging probe with affinity for cholesterol for differential fluorescent detection of human gallstones.METHODS: Cholesterol, mixed and pigment stones from cholecystectomy patients were incubated with hypericin or solvent. After 72 h, the stones were analysed for fluorescence(365 nm) and treated with 2-propanol/dimethyl sulfoxide for high performance liquid chromatography(HPLC) analysis. Rats with virtual gallbladder containing human cholesterol, mixed or pigment gallstones(VGHG) received 5 mg/kg hypericin or solvent and VGHG rats with cholesterol stones were given different hypericin doses(5-15 mg/kg). Twelve hours later, the stones were analysed at 365 nm. Biliary excretion and metabolites of hypericin were assessed in common bile duct(CBD) cannulated rats for 9 h using fluorospectrometry, HPLC and matrixassisted laser desorption/ionization-time-of-flight mass spectrometry(MALDI-TOF MS).RESULTS: Homogeneous high fluorescence was seen on cholesterol stones either pre-incubated with hypericin or extracted from VGHG rats receiving hypericin. Mixed stones showed a dotted fluorescent pattern, whereas pigment and solvent-treated ones lacked fluorescence. HPLC showed 7.68, 6.65 and 0.08 × 10^(-3) M of cholesterol in extracts from cholesterol, mixed, and pigment gallstones, respectively. Hypericin accounted for 2.0, 0.5 and 0.2 × 10-6 M in that order. On cholesterol stones from VGHG rats receiving different hypericin doses, a positive correlation was observed between dose and fluorescence. In the bile from CBD-cannulated rats, fluorescence represented 20% of the injected dose with two peaks in 9 h. HPLC analysis revealed that hypericin conjugates reached 60% of the peak area. By MALDI-TOF MS, hypericinglucuronide was detected. CONCLUSION: This study proves the potential use of hypericin for differential fluorescent detection of human gallstones regarding their chemical composition. | Marlein Miranda Cona Ye-Wei Liu Antoine Hubert Ting Yin Yuan-Bo Feng Peter de Witte Etienne Waelkens Yan-Sheng Jiang Jian Zhang Stefaan Mulier Qian Xia Gang Huang Raymond Oyen Yi-Cheng Ni | 2016 | World Journal of Gastroenterology2016，22，29： | 0 |
| 5 | A single-dose toxicity study on non-radioactive iodinated hypericin for a targeted anticancer therapy in mice显示文摘目的: Hypericin (忧郁) 和它的收音机衍生物为诊断、治疗学的目的与 ischemic 心疾病和恶意在动物模型被调查了。在 radioiodinated 忧郁前(123 我忧郁或 131 我忧郁) 能被看作临床上有用的药,它的 chemotoxicity 上的精力旺盛的评估是必要的。在现在的学习,我们检验了非放射性的 127 在为 24 h 和 14 d 的正常老鼠的我忧郁。 | Jun-jie LI Marlein Miranda CONA Yuan-bo FENG Feng CHEN Guo-zhi ZHANG Xue-bin FU Uwe HIMMELREICH Raymond OYEN Alfons VERBRUGGEN Yi-cheng NI | 2012 | Acta Pharmacologica Sinica2012，33，12： | 0 |
| 6 | Correlations between age, Charlson score and outcome in clinical unilateral T3a prostate cancer显示文摘根据泌尿学(香水) 指南的欧洲协会,一条预期寿命 > 10 年在前列腺癌症的治疗被认为一个重要因素。Charlson 分数被用来基于 comorbidities 预言死亡。这研究的目的是与 cT3a 前列腺癌症在病人调查在年龄, Charlson 分数和结果之间的关系。在 1987 和 2004 之间,并且谁与临床的 T3a 前列腺癌症的 200 个病人经历了激进的前列腺切除术(RP ) ,被数字直肠的检查(DRE ) 以前检测。病人们被分成二个年龄组(< 65 和 65 岁) 。病人们也根据 Charlson 分数被划分成二个组(= 0 和 1 ) 。年龄和 Charlson 分数关于他们病人的结果的预兆的力量被分析。吝啬的后续时期是 70.6 个月,并且病人的吝啬的年龄是 63.3 年。总共, 106 个病人是 < 65 岁和 94 个病人是 65 岁。年龄是全面幸存(OS ) 的一个重要预言者。0,并且 1 中的 20 个在 90 个病人在 110 个病人被发现的 Charlson。Charlson 分数不是生物化学的没有前进的幸存(BPFS ) 的一个重要预言者,临床的没有前进的幸存(CPFS ) 或 OS。考克斯 multivariate 分析证明那边缘地位是在 BPFS 的一个重要独立因素,并且癌症体积是在 CPFS 的一个重要独立因素。Charlson 分数不与临床的局部地先进的前列腺癌症在病人影响结果。年龄可以影响 OS。RP 能在激发的健康更老的病人被执行。然而,病人需要关于可能的外科相关的副作用被建议,例如尿不能自制和可勃起的机能障碍,它是年龄依赖者并且 comorbidity 依赖。 | Chao-Yu Hsu Steven Joniau Raymond Oyen Tania Roskams Hein Van Poppel | 2009 | Asian Journal of Andrology2009，11，1： | 0 |
| 7 | Separate calculation of DW-MRI in assessing therapeutic effect in liver tumors in rats显示文摘AIM:To explore whether the antitumor effect of a vascular disrupting agent(VDA)would be enhanced by combining with an antiangiogenic agent,and whether such synergistic effects can be effectively evaluated with separate calculation of diffusion weighted magnetic resonance imaging(DW-MRI).METHODS:Thirty-seven rats with implanted liver tumors were randomized into the following three groups:(1)ZD6126,a kind of VDA;(2)ZDTHA,ZD6126 in combination with an antiangiogenic,thalidomide;and(3)control.Morphological DW-MRI were performed and quantified before,4 h and 2 d after treatment.The apparent diffusion coefficient(ADC)values were calculated separately for low b values(ADC low),high b values(ADC high)and all b values(ADC all).The tissue perfusion contribution,ADC perf,was calculated as ADC low-ADC high.Imaging findings were finally verified by histopathology.RESULTS:The combination therapy with ZDTHA significantly delayed tumor growth due to synergistic effects by inducing cumulative tumor necrosis.In addition to delaying tumor growth,ZDTHA caused tumor necrosis in an additive manner,which was verified by HE staining.Although both ADC high and ADC all in the ZD6126and ZDTHA groups were significantly higher compared to those in the control group on day 2,the entire tumor ADC high of ZDTHA was even higher than that of ZD6126,but the significant difference was not observed for ADCall between ZDTHA and ZD6126.This indicated that the perfusion insensitive ADC high values calculated from high b value images performed significantly better than ADC all for the monitoring of tumor necrosis on day 2.The perfusion sensitive ADC perf derived from ADC low by excluding high b value effects could better reflect the reduction of blood flow due to the vessel shutdown induced by ZD6126,compared to the ADC low at 4 h.The ADC perf could provide valuable perfusion information from DW-MRI data.CONCLUSION:The separate calculation of ADC is more useful than conventional averaged ADC in evaluating the efficacy of combination therapy with ZD6126and thalidomide for solid tumors. | Feng Chen Frederik De Keyzer Yuan-Bo Feng Marlein Miranda Cona Jie Yu Guy Marchal Raymond Oyen Yi-Cheng Ni | 2013 | World Journal of Gastroenterology2013，19，47： | 0 |
| 8 | Intra-individual comparison of therapeutic responses to vascular disrupting agent CA4P between rodent primary and secondary liver cancers显示文摘AIM To compare therapeutic responses of a vascular-disrupting-agent, combretastatin-A4-phosphate(CA4 P), among hepatocellular carcinomas(HCCs) and implanted rhabdomyosarcoma(R1) in the same rats by magneticresonance-imaging(MRI), microangiography and histopathology.METHODS Thirty-six HCCs were created by diethylnitrosamine gavage in 14 rats that were also intrahepatically implanted with one R1 per rat as monitored by T2-/T1-weighted images(T2wI/T1wI) on a 3.0 T clinical MRIscanner. Vascular response and tumoral necrosis were detected by dynamic contrast-enhanced(DCE-) and CE-MRI before, 1 h after and 12 h after CA4P iv at 10 mg/kg(treatment group n = 7) or phosphate-buffered saline at 1.0 mL/kg(control group n = 7). Tumor blood supply was calculated by a semiquantitative DCE parameter of area under the time signal intensity curve(AUC30). In vivo MRI findings were verified by postmortem techniques.RESULTS On CE-T1wIs, unlike the negative response in all tumors of control animals, in treatment group CA4P caused rapid extensive vascular shutdown in all R1-tumors, but mildly or spottily in HCCs at 1 h. Consequently, tumor necrosis occurred massively in R1-tumors but patchily in HCCs at 12 h. AUC30 revealed vascular closure(66%) in R1-tumors at 1 h(P < 0.05), followed by further perfusion decrease at 12 h(P < 0.01), while less significant vascular clogging occurred in HCCs. Histomorphologically, CA4P induced more extensive necrosis in R1-tumors(92.6%) than in HCCs(50.2%)(P < 0.01); tumor vascularity heterogeneously scored +^+++ in HCCs but homogeneously scored ++ in R1-tumors.CONCLUSION This study suggests superior performance of CA4P in metastatic over primary liver cancers, which could guide future clinical applications of vascular-disruptingagents. | Ye-wei Liu Frederik De Keyzer Yuan-bo Feng Feng Chen Shao-li Song Johan Swinnen Guy Bormans Raymond Oyen Gang Huang Yi-cheng Ni | 2018 | World Journal of Gastroenterology2018，24，25： | 0 |
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