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| 1 | Promoter hypermethylation of CDH1, FHIT, MTAP and PLAGL1 in gastric adenocarcinoma in individuals from Northern Brazil显示文摘AIM: To evaluate the methylation status of CDH1, FHIT, MTAP and PLAGL1 promoters and the association of these findings with clinico-pathological characteristics. METHODS: Methylation-specific PCR (MSP) assay was performed in 13 nonneoplastic gastric adenocarcinoma, 30 intestinal-type gastric adenocarcinoma and 35 diffuse- type gastric adenocarcinoma samples from individuals in Northern Brazil. Statistical analyses were performed using the chi-square or Fisher’s exact test to assess associations between methylation status and clinico- pathological characteristics. RESULTS: Hypermethylation frequencies of CDH1, FHIT, MTAP and PLAGL1 promoter were 98.7%, 53.9%, 23.1% and 29.5%, respectively. Hypermethylation of three or four genes revealed a significant association with diffuse-type gastric cancer compared with nonneoplastic cancer. A higher hypermethylation frequency wassignificantly associated with H pylori infection in gastric cancers, especially with diffuse-type. Cancer samples without lymph node metastasis showed a higher FHIT hypermethylation frequency. MTAP hypermethylation was associated with H pylori in gastric cancer samples, as well as with diffuse-type compared with intestinal-type. In diffuse-type, MTAP hypermethylation was associated with female gender. CONCLUSION: Our findings show differential gene methylation in tumoral tissue, which allows us to conclude that hypermethylation is associated with gastric carcinogenesis. MTAP promoter hypermethylation can be characterized as a marker of diffuse-type gastric cancer, especially in women and may help in diagnosis, prognosis and therapies. The H pylori infectious agent was present in 44.9% of the samples. This infection may be correlated with the carcinogenic process through the gene promoter hypermethylation, especially the MTAP promoter in diffuse-type. A higher H pylori infection in diffuse-type may be due to greater genetic predisposition. | Mariana Ferreira Leal Eleonidas Moura Lima Patrícia Natália Oliveira Silva Paulo Pimentel Assumpo Danielle Queiroz Calcagno Spencer Luiz Marques Payo Rommel Rodríguez Burbano Marília de Arruda Cardoso Smith | 2007 | World Journal of Gastroenterology2007，13，18： | 24 |
| 2 | MYC and gastric adenocarcinoma carcinogenesis显示文摘MYC is an oncogene involved in cell cycle regulation,cell growth arrest,cell adhesion,metabolism,ribosome biogenesis,protein synthesis,and mitochondrial function. It has been described as a key element of several carcinogenesis processes in humans. Many studies have shown an association between MYC deregulation and gastric cancer. MYC deregulation is also seen in gastric preneoplastic lesions and thus it may have a role in early gastric carcinogenesis. Several studies have suggested that amplification is the main mechanism of MYC deregulation in gastric cancer. In the present review,we focus on the deregulation of the MYC oncogene in gastric adenocarcinoma carcinogenesis,including its association with Helicobacter pylori (H pylori) and clinical applications. | Danielle Queiroz Calcagno Mariana Ferreira Leal Paulo Pimentel Assumpo Marília de Arruda Cardoso Smith Rommel Rodríguez Burbano | 2008 | World Journal of Gastroenterology2008，14，39： | 8 |
| 3 | Clinical implication of 14-3-3 epsilon expression in gastric cancer显示文摘AIM:To evaluate for the first time the protein and mRNA expression of 14-3-3εin gastric carcinogenesis.METHODS:14-3-3εprotein expression was determined by western blotting,and mRNA expression was examined by real-time quantitative RT-PCR in gastric tumors and their matched non-neoplastic gastric tissue samples.RESULTS:Authors observed a significant reduction of 14-3-3εprotein expression in gastric cancer(GC)samples compared to their matched non-neoplastic tissue.Reduced levels of 14-3-3εwere also associated with diffuse-type GC and early-onset of this pathology.Our data suggest that reduced 14-3-3εmay have a role in gastric carcinogenesis process.CONCLUSION:Our results reveal that the reduced 14-3-3εexpression in GC and investigation of 14-3-3ε interaction partners may help to elucidate the carcino-genesis process. | Mariana Ferreira Leal Danielle Queiroz Calcagno Smia Demachki Paulo Pimentel Assumpo Roger Chammas Rommel Rodríguez Burbano Marília de Arruda Cardoso Smith | 2012 | World Journal of Gastroenterology2012，18，13： | 6 |
| 4 | Carebral and basilar artery thrombosis,following cervical spinal injury显示文摘 | Niedeggen A Rommel O Theile A | 1998 | Eentralbl Neurochir1998，60，1： | 1 |
| 5 | Cevebral and banilar artery thrembosis following cerrical spinal injury显示文摘 | Niedeggen A Rommel O Theile A | 1999 | J Neurochir1999，60，1： | 1 |
| 6 | Early diagnosis inpost-traumatic complex regional pain syndrome显示文摘 | Schurmann M Gradl G Rommel O | 2007 | Or-thopedics2007，30，6： | 1 |
| 7 | The directional feature of current transients,application in high-speed transmission-line protection显示文摘 | ROMMEL A FABIAN P EDUARDO O | 2013 | IEEE Transactions on Power Delivery2013，28，2： | 1 |
| 8 | Heparan sulfate proteoglycans interact exclusively with conformationally intact HPV L1 assemblies:basis for a virus-like particle ELISA显示文摘 | Rommel O Dillner J Fligge C | 2005 | J Med Virol2005，75，1： | 1 |
| 9 | Heparan sulfate proteoglycans interact exclusively with conformationally intact ttPV LI assemblies: basis for a virus-like particle ELISA显示文摘 | Rommel O Dillner J Fligge C | 2005 | J Med Virol2005，75，1： | 1 |
| 10 | STAT6-mediated BCL6 repression in primary mediastinal B-cell lymphoma (PMBL)显示文摘 | Ritz O Rommel K Dorsch K | 2013 | Oncotarget2013，4，： | 1 |
| 11 | Cerebral and basilar artery throm bosis,following cerviical spinal injury显示文摘 | Niiedeggen A Rommel O Theile A | 1998 | Entralbl Neruochir1998，60，1： | 1 |
| 12 | hTERT methylation and expression in gastric cancer显示文摘 | Carolina Oliveira Gigek Mariana Ferreira Leal Patricia Natalia Oliveira Silva Luara Carolina Frias Lisboa Eleonidas Moura Lima Danielle Queiroz Calcagno Paulo Pimentel Assump??o Rommel Rodriguez Burbano Marilia de Arruda Cardoso Smith | 2009 | Biomarkers2009，，8： | 1 |
| 13 | Cerebral and basilar artery thrombosis following cervical spinal injury显示文摘 | Niedeggen A Rommel O Theile A | 1999 | Zentralbl Neurochir1999，60，1： | 1 |
| 14 | The Role of Heart Failure Pharmacotherapy After Left Ventricular Assist Device Support显示文摘 | Rommel JJ O'Neill TJ Lishmanov A | 2014 | Heart Fail Clin2014，10，4： | 1 |
| 15 | Muscle pain in myophosphorylase deficiency (McArdle's disease):The role of gender,genotype,and pain-related coping显示文摘 | Rommel O Kley RA Dekomien G | 2006 | Pain2006，20，： | 1 |
| 16 | Establishment and conventional cytogenetic characterization of three gastric cancer cell lines显示文摘 | Mariana Ferreira Leal José Luiz Martins do Nascimento Carla Elvira Araújo da Silva Maria Fernanda Vita Lamar?o Danielle Queiroz Calcagno André Salim Khayat Paulo Pimentel Assump??o Isabel Rosa Cabral Marília de Arruda Cardoso Smith Rommel Rodríguez Burban | 2009 | Cancer Genetics and Cytogenetics2009，，1： | 1 |
| 17 | The plasma membrane H^+ -ATPase from the biotrophie rust fungus Uromyces fabae: molecular characterization of the gene (PMA 1 ) and functional expression of the enzyme in yeast 显示文摘 | Struck C Siebels C Rommel O | 1998 | Mol Plant Microbe interact1998，11，： | 1 |
| 18 | Quantitative sensorytesting,neurophysiological and psychological examination in pa-tients with complex regional pain syndrome and hemisensorydeficits显示文摘 | Rommel O Malin J P Zenz M Jnig W | 2001 | Pain2001，93，： | 1 |
| 19 | Reference genes for quantitative RT-PCR data in gastric tissues and cell lines显示文摘AIM:To evaluate the suitability of reference genes in gastric tissue samples and cell lines.METHODS:The suitability of genes ACTB,B2M,GAPDH,RPL29,and 18S rRNA was assessed in21 matched pairs of neoplastic and adjacent nonneoplastic gastric tissues from patients with gastric adenocarcinoma,27 normal gastric tissues from patients without cancer,and 4 cell lines using reverse transcription quantitative real-time polymerase chain reaction(RT-qPCR).The ranking of the best single and combination of reference genes was determined by NormFinder,geNorm,BestKeeper,and DataAssist.In addition,GenEx software was used to determine the optimal number of reference genes.To validate the results,the mRNA expression of a target gene,DNMT1,was quantified using the different reference gene combinations suggested by the various software packages for normalization.RESULTS:ACTB was the best reference gene for all gastric tissues,cell lines and all gastric tissues plus cell lines.GAPDH+B2M or ACTB+B2M was the best combination of reference genes for all the gastric tissues.On the other hand,ACTB+B2M was the best combination for all the cell lines tested and was also the best combination for analyses involving all the gastric tissues plus cell lines.According to the GenEx software,2 or 3 genes were the optimal number of references genes for all the gastric tissues.The relative quantification of DNMT1 showed similar patterns when normalized by each combination of reference genes.The level of expression of DNMT1 in neoplastic,adjacent non-neoplastic and normal gastric tissues did not differ when these samples were normalized using GAPDH+B2M(P=0.32),ACTB+B2M(P=0.61),or GAPDH+B2M+ACTB(P=0.44).CONCLUSION:GAPDH+B2M or ACTB+B2M is the best combination of reference gene for all the gastric tissues,and ACTB+B2M is the best combination for the cell lines tested. | Fernanda Wisnieski Danielle Queiroz Calcagno Mariana Ferreira Leal Leonardo Caires dos Santos Carolina de Oliveira Gigek Elizabeth Suchi Chen Thaís Brilhante Pontes Paulo Pimentel Assumpo Mnica Barauna de Assumpo Smia Demachki Rommel Rodríguez Burbano Marília de Arruda Cardoso Smith | 2013 | World Journal of Gastroenterology2013，19，41： | 0 |
| 20 | Tale of two kinases:Protein kinase A and Ca^(2+)/calmodulin-dependent protein kinase Ⅱ in pre-diabetic cardiomyopathy显示文摘Metabolic syndrome is a pre-diabetic state characterized by several biochemical and physiological alterations,including insulin resistance,visceral fat accumulation,and dyslipidemias,which increase the risk for developing cardiovascular disease.Metabolic syndrome is associated with augmented sympathetic tone,which could account for the etiology of pre-diabetic cardiomyopathy.This review summarizes the current knowledge of the pathophysiological consequences of enhanced and sustainedβ-adrenergic response in pre-diabetes,focusing on cardiac dysfunction reported in diet-induced experimental models of pre-diabetic cardiomyopathy.The research reviewed indicates that both protein kinase A and Ca^(2+)/calmodulin-dependent protein kinase Ⅱ play important roles in functional responses mediated byβ1-adrenoceptors;therefore,alterations in the expression or function of these kinases can be deleterious.This review also outlines recent information on the role of protein kinase A and Ca^(2+)/calmodulin-dependent protein kinase Ⅱ in abnormal Ca^(2+)handling by cardiomyocytes from diet-induced models of pre-diabetic cardiomyopathy. | Pamela Gaitán-González Rommel Sánchez-Hernández José-Antonio Arias-Montaño Angélica Rueda | 2021 | World Journal of Diabetes2021，12，10： | 0 |
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