|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | RAB31 marks and controls an ESCRT-independent exosome pathway显示文摘Exosomes are generated within the multivesicular endosomes(MVEs)as intraluminal vesicles(ILVs)and secreted during the fusion of MVEs with the cell membrane.The mechanisms of exosome biogenesis remain poorly explored.Here we identify that RAB31 marks and controls an ESCRT-independent exosome pathway.Active RAB31,phosphorylated by epidermal growth factor receptor(EGFR),engages flotillin proteins in lipid raft microdomains to drive EGFR entry into MVEs to form ILVs,which is independent of the ESCRT(endosomal sorting complex required for transport)machinery.Active RAB31 interacts with the SPFH domain and drives ILV formation via the Flotillin domain of flotillin proteins.Meanwhile,RAB31 recruits GTPase-activating protein TBC1D2B to inactivate RAB7,thereby preventing the fusion of MVEs with lysosomes and enabling the secretion of ILVs as exosomes.These findings establish that RAB31 has dual functions in the biogenesis of exosomes:driving ILVs formation and suppressing MVEs degradation,providing an exquisite framework to better understand exosome biogenesis. | Denghui Wei Weixiang Zhan Ying Gao Liyan Huang Run Gong Wen Wang Ruhua Zhang Yuanzhong Wu Song Gao Tiebang Kang | 2021 | Cell Research2021，31，2： | 12 |
| 2 | hSSB1 regulates both the stability and the transcriptional activity of p53显示文摘肿瘤 suppressor p53 为涉及对多样的 genotoxic 应力的反应的几个细胞的过程是必要的,包括房间周期拘捕, DNA 修理, apoptosis 和老朽。p53 的规定的研究主要集中了于它的稳定性和 transactivation;然而,为 p53 的新规章的分子经常也被识别了。这里,我们报导那人的 ssDNA 绑定蛋白质 SSB1 (hSSB1 ) ,新奇 DNA 联系损坏的蛋白质,能与 p53 交往并且保护 p53 免受调停 ubiquitin 的降级的伤害。而且, hSSB1 也与 acetyltransferase p300 联系并且被在 lysine382 影响 p53 的 acetylation 为 p53 目标基因 p21 的有效 transcriptional 激活要求。机能上地, G2/M 检查点的 hSSB1 击倒导致废除部分依赖于 p53 或 p300。一起,我们的结果显示那 hSSB1 可以调整 DNA 损坏检查点由断然 modulating p53 和它的下游的目标 p21。 | Shuangbing Xu Yuanzhong Wu Qiong Chen Jingying Cao Kaishun Hu Jianjun Tang Yi Sang Fenju Lai Li Wang Ruhua Zhang Sheng-Ping Li Yi-Xin Zeng Yuxin Yina Tiebang Kang | 2013 | Cell Research2013，23，3： | 7 |
| 3 | The susceptibility gene for familial nasopharyngeal carcinoma is mapped on chromosome 4p11-p14 by haplotype analyses显示文摘In our previous study, one candidate suscepti-bility locus for familial nasopharyngeal carcinoma (NPC) has been defined to a 14.21-cM region on 4p15.1-q12, whereas the distal minimum boundary of this region remained to be further determined in respect that the two markers D4S2996 and D4S428 were uninformative. In the present study, we carried out a haplotype analysis to identify the exact bound-ary by using the combination of a set of microsatellite mark-ers and single nucleotide polymorphism (SNP) markers in two major NPC families. We defined the exact distal bound-ary between D4S1577 and D4S3347, and consequently shortened the susceptibility locus to an 8.29-cM segment on 4p11-p14. | CHEN Hankui, FENG Bingjian, LIANG Hui, ZHANG Ruhua & ZENG Yixin Cancer Center, Sun Yet-sun University, Guangzhou 510060, China Correspondence should be addressed to Zeng Yixin (e-mail: yxzeng@ gzsums.edu.cn) | 2003 | Chinese Science Bulletin2003，48，21： | 2 |
| 4 | Systematic screening for potential therapeutic targets in osteosarcoma through a kinome-wide CRISPR-Cas9 library显示文摘Objective:Osteosarcoma is the most common primary malignant bone tumor.However,the survival of patients with osteosarcoma has remained unchanged during the past 30 years,owing to a lack of efficient therapeutic targets.Methods:We constructed a kinome-targeting CRISPR-Cas9 library containing 507 kinases and 100 nontargeting controls and screened the potential kinase targets in osteosarcoma.The CRISPR screening sequencing data were analyzed with the Model-based Analysis of Genome-wide CRISPR/Cas9 Knockout(MAGeCK)Python package.The functional data were applied in the 143B cell line through lenti-CRISPR-mediated gene knockout.The clinical significance of kinases in the survival of patients with osteosarcoma was analyzed in the R2:Genomics Analysis and Visualization Platform.Results:We identified 53 potential kinase targets in osteosarcoma.Among these targets,we analyzed 3 kinases,TRRAP,PKMYT1,and TP53RK,to validate their oncogenic functions in osteosarcoma.PKMYT1 and TP53RK showed higher expression in osteosarcoma than in normal bone tissue,whereas TRRAP showed no significant difference.High expression of all 3 kinases was associated with relatively poor prognosis in patients with osteosarcoma.Conclusions:Our results not only offer potential therapeutic kinase targets in osteosarcoma but also provide a paradigm for functional genetic screening by using a CRISPR-Cas9 library,including target design,library construction,screening workflow,data analysis,and functional validation.This method may also be useful in potentially accelerating drug discovery for other cancer types. | Yuanzhong Wu Liwen Zhou Zifeng Wang Xin Wang Ruhua Zhang Lisi Zheng Tiebang Kang | 2020 | Cancer Biology & Medicine2020，17，3： | 2 |
| 5 | Room temperature ozone sensing properties of p-type CuCrO 2 nanocrystals显示文摘 | Shu Zhou Xiaodong Fang Zanhong Deng Da Li Weiwei Dong Ruhua Tao Gang Meng Tao Wang | 2009 | Sensors & Actuators: B Chemical2009，，1： | 1 |
| 6 | Synthesis of CuAlO2 Ceramics using sol-gel 显示文摘 | Deng Zanhong Zhu Xuebin Tao Ruhua | 2007 | Materials Letters2007，61，3： | 1 |
| 7 | Hydrothermal synthesis and characterization of CuCrO 2 laminar nanocrystals显示文摘 | Shu Zhou Xiaodong Fang Zanhong Deng Da Li Weiwei Dong Ruhua Tao Gang Meng Tao Wang Xuebin Zhu | 2008 | Journal of Crystal Growth2008，，24： | 1 |
| 8 | A novel approach to titania nanowire ar-rays as photoanodes of back-illuminated dye-sensitized solarcells显示文摘 | Tao Ruhua | 2010 | J Power Sources2010，195，： | 1 |
| 9 | Sn Online Adaptive Neuro-Fuzzy Power System Stabilizer for Multimachine Systems显示文摘 | You Ruhua Eghbali H J Nehrir M H | 2003 | Power Systems IEEE Transactions on2003，18，1： | 1 |
| 10 | The application of fiber optic sensor to the study of engineering structure显示文摘 | Zhu Qirong Fang Ruhua Yang Guobiao | 2006 | Key Engineering Materials:Experimental Mechanics in Nano and Biotechnology2006，，： | 1 |
| 11 | An Online Adaptive Neuro-Fuzzy Power System Stabilizer for Multimachine Systems 显示文摘 | Ruhua You Hassan J Eghbali M | 2003 | IEEE Transactions on Power Systems2003，18，1： | 1 |
| 12 | Finite element analysis of material in-terfacial fracture intensity in porcelain-fused-to-metal restoration显示文摘 | Mi Honglin Fang Ruhua | 2005 | Journal of Tongji University2005，33，1： | 1 |
| 13 | Study on t he Kinetics of Nisin Fermentation 显示文摘 | Xie Fengchu Yang Bo Yao Ruhua | 2001 | Journal of South China University of Technology ( Natural Science Edition)2001，29，5： | 1 |
| 14 | Rab22a-NeoF1 fusion protein promotes osteosarcoma lung metastasis through its secretion into exosomes显示文摘It remains unknown for decades how some of the therapeutic fusion proteins positive in a small percentage of cancer cells account for patient outcome.Here,we report that osteosarcoma Rab22a-NeoF1 fusion protein,together with its binding partner PYK2,is sorted into exosomes by HSP90 via its KFERQ-like motif(RVLFLN^(142)).The exosomal Rab22a-NeoF1 fusion protein facilitates the pulmonary pre-metastatic niche formation by recruiting bone marrow-derived macrophages.The exosomal PYK2 activates RhoA in its negative recipient osteosarcoma cells and induces signal transducer and activator of transcription 3 activation in its recipient macrophages to increase M2 phenotype.Consequently,lung metastases of its recipient osteosarcoma cells are promoted by this exosomal Rab22a-NeoF1 fusion protein,and this event can be targeted by disrupting its interaction with PYK2 using a designed internalizing RGD peptide. | Li Zhong Dan Liao Jingjing Li Wenqiang Liu Jingxuan Wang Cuiling Zeng Xin Wang Zhiliang Cao Ruhua Zhang Miao Li Kuntai Jiang Yi-Xin Zeng Jianhua Sui Tiebang Kang | 2021 | Signal Transduction and Targeted Therapy2021，6，3： | 1 |
| 15 | Optimized Design for the Plow of a Submarine Plowing Trencher显示文摘耕作 trencher 的潜水艇的犁是主要功能的机制之一,并且它的优化是很重要的。设计参数在决定一个容器的拖引的力量的要求起一个很重要的作用。一个多客观的基因算法在获得犁的最佳的设计的努力基于犁表面的分析模型被检验了并且适用。为一个特定的土壤条件,草稿力量和是在犁的工作过程的关键参数的犁板表面区域被发现犁片穿入角度的相应最佳的价值和犁的主要的切片的二个表面角度优化。象偏差,犁板电梯角度,正切线的尖变化,和跨越的长度的犁板方面角度那样的参数也沿着土壤流动方向关于犁板表面的力量被分析。结果证明优化的犁有改进的犁表演。分别地,主要的切片和犁板的草稿力量是 10.6% 和 7% 不到原来的设计。犁板的 Gaussian 弯曲的标准差被 64.5% 降低,它暗示优化犁板表面的光滑比原版大得多。 | Hanyang Gong Ruhua Yuan Xiaodong Xing Liquan Wang Zhipeng Wang Haixia Gong | 2013 | Journal of Marine Science and Application2013，12，1： | 1 |
| 16 | Study on interracial fracture behaviors of the porcelain-fused-to-metalrestoration by moir6 interferometry method and finite element analysis显示文摘 | Ruhua Fang Dongmei Wang Xiuyin Zhang | 2001 | The International Conference onExperimetal Mechanics(SPIE)2001，4537，： | 1 |
| 17 | SUMOylation stabilizes hSSB1 and enhances the recruitment of NBS1 to DNA damage sites显示文摘Human single-stranded DNA-binding protein 1(hSSB1)is required for the efficient recruitment of the MRN complex to DNA doublestrand breaks and is essential for the maintenance of genome integrity.However,the mechanism by which hSSB1 recruits NBS1 remains elusive.Here,we determined that hSSB1 undergoes SUMOylation at both K79 and K94 under normal conditions and that this modification is dramatically enhanced in response to DNA damage.SUMOylation of hSSB1,which is specifically fine-tuned by PIAS2α,and SENP2,not only stabilizes the protein but also enhances the recruitment of NBS1 to DNA damage sites.Cells with defective hSSB1 SUMOylation are sensitive to ionizing radiation,and global inhibition of SUMOylation by either knocking out UBC9 or adding SUMOylation inhibitors significantly enhances the sensitivity of cancer cells to etoposide.Our findings reveal that SUMOylation,as a novel posttranslational modification of hSSB1,is critical for the functions of this protein,indicating that the use of SUMOylation inhibitors(e.g.,2-D08 and ML-792)may be a new strategy that would benefit cancer patients being treated with chemo-or radiotherapy. | Liwen Zhou Lisi Zheng Kaishun Hu Xin Wang Ruhua Zhang Yezi Zou Li Zhong Shang Wang Yuanzhong Wu Tiebang Kang | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 1 |
| 18 | Study on the bioavailability of baicalin-phospholipid complex by using HPLC显示文摘 | WU Jianmei CHEN Dawei ZHANG Ruhua | 1999 | Biomedical Chromatography1999，13，7： | 1 |
| 19 | Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity显示文摘STING,an endoplasmic reticulum(ER)transmembrane protein,mediates innate immune activation upon cGAMP stimulation and is degraded through autophagy.Here,we report that activated STING could be transferred between cells to promote antitumor immunity,a process triggered by RAB22A-mediated non-canonical autophagy.Mechanistically,RAB22A engages PI4K2A to generate PI4P that recruits the Atg12–Atg5–Atg16L1 complex,inducing the formation of ER-derived RAB22A-mediated non-canonical autophagosome,in which STING activated by agonists or chemoradiotherapy is packaged.This RAB22A-induced autophagosome fuses with RAB22A-positive early endosome,generating a new organelle that we name Rafeesome(RAB22A-mediated non-canonical autophagosome fused with early endosome).Meanwhile,RAB22A inactivates RAB7 to suppress the fusion of Rafeesome with lysosome,thereby enabling the secretion of the inner vesicle of the autophagosome bearing activated STING as a new type of extracellular vesicle that we define as R-EV(RAB22A-induced extracellular vesicle).Activated STING-containing R-EVs induce IFNβrelease from recipient cells to the tumor microenvironment,promoting antitumor immunity.Consistently,RAB22A enhances the antitumor effect of the STING agonist diABZI in mice,and a high RAB22A level predicts good survival in nasopharyngeal cancer patients treated with chemoradiotherapy.Our findings reveal that Rafeesome regulates the intercellular transfer of activated STING to trigger and spread antitumor immunity,and that the inner vesicle of non-canonical autophagosome originated from ER is secreted as R-EV,providing a new perspective for understanding the intercellular communication of organelle membrane proteins. | Ying Gao Xueping Zheng Boyang Chang Yujie Lin Xiaodan Huang Wen Wang Shirong Ding Weixiang Zhan Shang Wang Beibei Xiao Lanqing Huo Youhui Yu Yilin Chen Run Gong Yuanzhong Wu Ruhua Zhang Li Zhong Xin Wang Qiuyan Chen Song Gao Zhengfan Jiang Denghui Wei Tiebang Kang | 2022 | Cell Research2022，32，12： | 1 |
| 20 | Preparation of the Traditinal Chinese Medicine Compound Recipe Heart-Protecting Musk pH-Dependent Gradient-Release显示文摘 | Hongtao Song Tao Guo Ruhua Zhang | 2002 | Drug Development and Industrial Pharmacy2002，28，10： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员