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您的检索式：作者名="Ruijie Wan"
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| 1 | Osteogenic and antibacterial dual functions of a novel levofloxacin loaded mesoporous silica microspheres/nano-hydroxyapatite/ polyurethane composite scaffold显示文摘Lev/MSNs/n-HA/PU has been proved to be a novel scaffold material to treat bone defect caused by chronic osteomyelitis.We have previously identified that this material can effectively treat chronic osteomyelitis caused by Staphylococcus aureus in vivo.However,the potential mechanisms of antibacterial and osteogenic induction properties remain unclear.Thus,for osteogenesis property,immunohistochemistry,PCR,and Western blot were performed to detect the expression of osteogenic markers.Furthermore,flow cytometry and TUNEL were applied to analyze MC3T3-E1 proliferation and apoptosis.For antibacterial property,the material was co-cultivated with bacteria,bacterial colony forming units was counted and the release time of the effective levofloxacin was assayed by agar disc-diffusion test.Moreover,scanning electron microscope was applied to observe adhesion of bacteria.In terms of osteogenic induction,we found BMSCs adherently grew more prominently on Lev/MSNs/n-HA/PU.Lev/MSNs/n-HA/PU also enhanced the expression of osteogenic markers including OCN and COL1a1,as well as effectively promoted the transition from G1 phase to G2 phase.Furthermore,Lev/MSNs/n-HA/PU could reduce apoptosis of MC3T3-E1.Besides,both Lev/MSNs/n-HA/PU and n-HA/PU materials could inhibit bacterial colonies,while Lev/MSNs/n-HA/PU possessed a stronger antibacterial activities,and lower bacterial adhesion than n-HA/PU.These results illustrated that Lev/MSNs/n-HA/PU composite scaffold possess favorable compatibility in vitro,which induce osteogenic differentiation of MSCs,promote proliferation and differentiation of MC3T3-E1,and inhibit apoptosis.Moreover,clear in vitro antibacterial effect of Lev/MSNs/n-HA/PU was also observed.In summary,this study replenishes the potential of Lev/MSNs/n-HA/PU composite scaffold possess dual functions of anti-infection and enhanced osteogenesis for future clinical application. | Zhiping Kuang Guangming Dai Ruijie Wan Dongli Zhang Chen Zhao Cheng Chen Jidong Li Hongchen Gu Wei Huang | 2021 | Genes & Diseases2021，8，2： | 4 |
| 2 | A temperature responsive adhesive hydrogel for fabrication of flexible electronic sensors显示文摘Flexible electronics are playing an increasingly important role in human health monitoring and healthcare diagnosis.Strong adhesion on human tissue would be ideal for reducing interface resistance and motion artifacts,but arising problems such as skin irritation,rubefaction,and pain upon device removal have hampered their utility.Here,inspired by the temperature reversibility of hydrogen bonding,a skin-friendly conductive hydrogel with multiple-hydrogen bonds was designed by using biocompatible poly(vinyl alcohol)(PVA),phytic acid(PA),and gelatin(Gel).The obtained PVA/PA/Gel(PPG)hydrogel with temperature-triggered tunable mechanic could reliably adhere to skin and detect electrophysiological signals under a hot compress while be readily removed under a cool compress.Furthermore,the additional advantages of transparency,breathability,and antimicrobial activity of the PPG hydrogel ensure its long-time wearable value on the skin.It is both environmentally friendly and cost saving for the waste PPG hydrogel during production can be recycled based on their reversible physical bonding.The PPG hydrogel sensor is expected to have good application prospects to record electrophysiological signals in human health monitoring. | Wan Liu Ruijie Xie Jingyu Zhu Jiansheng Wu Junfeng Hui Xiaoyan Zheng Fengwei Huo Daidi Fan | 2022 | npj Flexible Electronics2022，6，1： | 2 |
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