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| 1 | SARS-CoV-2 cell entry and targeted antiviral development显示文摘Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is the causative agent of the pandemic coronavirus disease 2019(COVID-19),which threatens human health and public safety.In the urgent campaign to develop anti-S ARS-CoV-2 therapies,the initial entry step is one of the most appealing targets.In this review,we summarize the current understanding of SARS-CoV-2 cell entry,and the development of targeted antiviral strategies.Moreover,we speculate upon future directions toward nextgeneration of SARS-CoV-2 entry inhibitors during the upcoming post-pandemic era. | Zinuo Chen Ruikun Du Jazmin M.Galvan Achi Lijun Rong Qinghua Cui | 2021 | Acta Pharmaceutica Sinica B2021，11，12： | 3 |
| 2 | Allopregnanolone targets nucleoprotein as a novel influenza virus inhibitor显示文摘Influenza A virus(IAV)poses a global public health concern and remains an imminent threat to human health.Emerging antiviral resistance to the currently approved influenza drugs emphasizes the urgent need for new therapeutic entities against IAV.Allopregnanolone(ALLO)is a natural product that has been approved as an antidepressant drug.In the present study,we repurposed ALLO as a novel inhibitor against IAVs.Mechanistic studies demonstrated that ALLO inhibited virus replication by interfering with the nucleus translocation of viral nucleoprotein(NP).In addition,ALLO showed significant synergistic activity with compound 16,a hemagglutinin inhibitor of IAVs.In summary,we have identified ALLO as a novel influenza virus inhibitor targeting NP,providing a promising candidate that deserves further investigation as a useful anti-influenza strategy in the future. | Meiyue Dong Yanyan Wang Ping Li Zinuo Chen Varada Anirudhan Qinghua Cui Lijun Rong Ruikun Du | 2023 | Virologica Sinica2023，38，6： | 0 |
| 3 | Characterization of the viral fibroblast growth factor homolog of Helicoverpa armigera single nucleopolyhedrovirus显示文摘Fibroblast growth factor(FGF) is found throughout multicellular organisms; however, fgf homologs(vfgf) have only been identified among viruses in lepidopteran baculoviruses. The function of v FGFs from Group I alphabaculoviruses, including Autographa californica multiple nucleopolyhedrovirus(Ac MNPV) and Bombyx mori nucleopolyhedrovirus(Bm NPV), involves accelerated killing of infected larvae by both viruses. The v FGF of Group II alphabaculovirus is structurally different from that of Group I alphabaculovirus, with a larger C-terminal region and additional N-linked glycosylation sites. In this study, we characterized the Group II alphabaculovirus v FGF of Helicoverpa armigera single nucleopolyhedrovirus(Hear NPV). The transcription and expression of vfgf was detected at 3 h and 16 h post-infection in Hear NPV-infected cells. To further study v FGF function, we constructed vfgf-knockout and-repaired Hear NPV bacmids and investigated their affect in both cultured cells and insects. Deletion of vfgf had no effect on budded-virus production or viral DNA replication in cultured Hz AM1 cells. However, bioassays showed that Hear NPV vfgf deletion significantly increased the median lethal dose and delayed the median lethal time by ~12 h in the host insect when the virus was delivered orally. These results suggested that v FGF is an important virulent factor for HearN PV infection and propagation in vivo. | Feifei Yin Ruikun Du Wenhua Kuang Guang Yang Hualin Wang Fei Deng Zhihong Hu Manli Wang | 2016 | Virologica Sinica2016，31，3： | 0 |
| 4 | Optimization and applications of an in vivo bioluminescence imaging model of influenza A virus infections显示文摘Dear Editor,In vivo bioluminescence imaging(BLI)models of virus infection possess unique advantages over conventional assays.For instance,the BLI model enables rapid and real-time detection of viral load and dissemination in the same animal over time(Mehle,2015;Wen et al.,2022). | Xiaojing Lin Murong Zhu Xiujuan Zhao Longlong Si Meiyue Dong Varada Anirudhan Qinghua Cui Lijun Rong Ruikun Du | 2023 | Virologica Sinica2023，38，4： | 0 |
| 5 | An in vitro recombination-based reverse genetic system for rapid mutagenesis of structural genes of the Japanese encephalitis virus显示文摘Japanese encephalitis virus(JEV) is one of the most common pathogens of severe viral encephalitis, which is a severe threat to human health. Despite instability of the JEV genome in bacteria, many strategies have been developed to establish molecular clone systems of JEV, providing convenient tools for studying the virus life cycle and virus–host interactions. In this study, we adapted an In-Fusion enzyme-based in vitro recombination method to construct a reverse genetic system of JEV, thereby providing a rapid approach to introduce mutations into the structural genes. A truncated genome without the structural genes was constructed as the backbone, and the complementary segment containing the structural genes was recombined in vitro, which was then transfected directly into virus-permissive cells. The progeny of the infectious virus was successfully detected in the supernatant of the transfected cells, and showed an identical phenotype to its parental virus. To provide a proof-of-principle, the 12 conserved cysteine residues in the envelope(E) protein of JEV were respectively mutated using this approach, and all mutations resulted in a complete failure to generate infectious virus. However, a leucine-tophenylanine mutation at amino acid 107 of the E protein did not interfere with the production of the infectious virus. These results suggested that all 12 cysteines in the E protein are essential for the JEV life cycle. In summary, a novel reverse genetic system of JEV was established for rapidly introducing mutations into structural genes, which will serve as a useful tool for functional studies. | Ruikun Du Manli Wang Zhihong Hu Hualin Wang Fei Deng | 2015 | Virologica Sinica2015，30，5： | 0 |
| 6 | Revisiting influenza A virus life cycle from a perspective of genome balance显示文摘Influenza A virus(IAV)genome comprises eight negative-sense RNA segments,of which the replication is well orchestrated and the delicate balance of multiple segments are dynamically regulated throughout IAV life cycle.However,previous studies seldom discuss these balances except for functional hemagglutinin-neuraminidase balance that is pivotal for both virus entry and release.Therefore,we attempt to revisit IAV life cycle by highlighting the critical role of“genome balance”.Moreover,we raise a“balance regression”model of IAV evolution that the virus evolves to rebalance its genome after reassortment or interspecies transmission,and direct a“balance compensation”strategy to rectify the“genome imbalance”as a result of artificial modifications during creation of recombinant IAVs.This review not only improves our understanding of IAV life cycle,but also facilitates both basic and applied research of IAV in future. | Ruikun Du Qinghua Cui Zinuo Chen Xiujuan Zhao Xiaojing Lin Lijun Rong | 2023 | Virologica Sinica2023，38，1： | 0 |
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