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| 1 | Vascularization and osteogenesis in ectopically implanted bone tissue-engineered constructs with endothelial and osteogenic differentiated adipose-derived stem cells显示文摘BACKGROUND A major problem in the healing of bone defects is insufficient or absent blood supply within the defect.To overcome this challenging problem,a plethora of approaches within bone tissue engineering have been developed recently.Bearing in mind that the interplay of various diffusible factors released by endothelial cells(ECs)and osteoblasts(OBs)have a pivotal role in bone growth and regeneration and that adjacent ECs and OBs also communicate directly through gap junctions,we set the focus on the simultaneous application of these cell types together with platelet-rich plasma(PRP)as a growth factor reservoir within ectopic bone tissue engineering constructs.AIM To vascularize and examine osteogenesis in bone tissue engineering constructs enriched with PRP and adipose-derived stem cells(ASCs)induced into ECs and OBs.METHODS ASCs isolated from adipose tissue,induced in vitro into ECs,OBs or just expanded were used for implant construction as followed:BPEO,endothelial and osteogenic differentiated ASCs with PRP and bone mineral matrix;BPUI,uninduced ASCs with PRP and bone mineral matrix;BC(control),only bone mineral matrix.At 1,2,4 and 8 wk after subcutaneous implantation in mice,implants were extracted and endothelial-related and bone-related gene expression were analyzed,while histological analyses were performed after 2 and 8 wk.RESULTS The percentage of vascularization was significantly higher in BC compared to BPUI and BPEO constructs 2 and 8 wk after implantation.BC had the lowest endothelial-related gene expression,weaker osteocalcin immunoexpression and Spp1 expression compared to BPUI and BPEO.Endothelial-related gene expression and osteocalcin immunoexpression were higher in BPUI compared to BC and BPEO.BPEO had a higher percentage of vascularization compared to BPUI and the highest CD31 immunoexpression among examined constructs.Except Vwf,endothelial-related gene expression in BPEO had a later onset and was upregulated and well-balanced during in vivo incubation that induced late onset of Spp1 expression and pronounced osteocalcin immunoexpression at 2 and 8 wk.Tissue regression was noticed in BPEO constructs after 8 wk.CONCLUSION Ectopically implanted BPEO constructs had a favorable impact on vascularization and osteogenesis,but tissue regression imposed the need for discovering a more optimal EC/OB ratio prior to considerations for clinical applications. | Jelena G Najdanović Vladimir J Cvetković Sanja T Stojanović MarijaĐVukelić-Nikolić Jelena MŽivković Stevo J Najman | 2021 | World Journal of Stem Cells2021，13，1： | 3 |
| 2 | Dynamic mechanical and thermal properties of MAPE treated jute/HDPE compos- ites显示文摘 | SMITA M SUSHIL K V SANJA K N | 2006 | Compos Sci Technol2006，66，： | 1 |
| 3 | Dynam- ics of steam accumulation 显示文摘 | VLADIMIR D S BLAZENKA M SANJA P | 2012 | Applied Thermal Engi- neering2012，37，： | 1 |
| 4 | Inflammation,oxidative stress and repair capacity of the vascular endothelium in obstructive sleep apnea显示文摘 | Sanja J Margherita P Steven M K | 2008 | Circulation2008，117，： | 1 |
| 5 | Dynamic mechanical and ther- mal properties of MAPE treated jute/HDPE composites显示文摘 | Smita M Sushil K V Sanja K N | 2006 | Compos Sci Technol2006，66，： | 1 |
| 6 | Efficient numerical method for district heating system hydraulics显示文摘 | Vladimir DS Sanja P Blazenka M Branislav Z Srdjan N | | 0，，： | 1 |
| 7 | Clinical comparasion of the quality of fissure sealing with fisural and ionosit-seal in the observation period of up to two years显示文摘 | Lidija M Dragan M Sanja M | 2010 | Scientific Journal of the Faculty of Medicine2010，27，3： | 1 |
| 8 | Modeling Cellular Pharmacokinetics of 14-and 15-Membered Macrolides with Physicochemical Properties显示文摘 | Vinja S Sanja K Ivica M | 2011 | Journal of medicinal Chemistry2011，54，3： | 1 |
| 9 | Pharmacological intervention in age-associated brain disorders by Flupirtine: Alzheimer’s and Prion diseases显示文摘 | Sanja Perovic Markus B?hm Erik Meesters Andrea Meinhardt Gabriela Pergande Werner E.G Müller | 1998 | Mechanisms of Ageing and Development1998，，1： | 1 |
| 10 | Effect of Flupirtine on Bcl-2 and Glutathione Level in Neuronal Cells Treated in Vitro with the Prion Protein Fragment (PrP106-126)显示文摘 | Sanja Perovic Heinz C. Schr?der Gabriela Pergande Hiroshi Ushijima Werner E.G. Müller | 1997 | Experimental Neurology1997，，2： | 1 |
| 11 | Thermodynamic characterization of metal dissolution and inhibitor adsorption processes in the low carbon steel/mimosa tannin/sulfuric acid system 显示文摘 | SANJA M IVICA S | 2002 | Appl Surf Sci2002，199，： | 1 |
| 12 | Inflammation, Oxidative Stress, and Repair Capacity of the Vascular Endothelium in Ob- structive Sleep Apnea 显示文摘 | Jelic Sanja Padeletti Margherita Kawut Steven M | 2008 | Circulation2008，117，17： | 1 |
| 13 | Thermodynamic characterization of metal dissolution and inhibitor adsorption processes in the low carbon steel/mimosa tannin/sulfuric acid system显示文摘 | SANJA M IVICA S | | 0，，： | 1 |
| 14 | Molecular control of serial module formation along the apical–basal axis in the sponge Lubomirskia baicalensis: silicateins, mannose-binding lectin and mago nashi显示文摘 | Matthias Wiens Sergey I. Belikov Oxana V. Kaluzhnaya Anatoli Krasko Heinz C. Schr?der Sanja Perovic-Ottstadt Werner E. G. Müller | 2006 | Development Genes and Evolution2006，，5： | 1 |
| 15 | Differentiation capacity of epithelial cells in the sponge Suberites domuncula显示文摘 | Heinz C. Schr?der Sanja Perovi?-Ottstadt Matthias Wiens Renato Batel Isabel M. Müller Werner E. G. Müller | 2004 | Cell and Tissue Research2004，，2： | 1 |
| 16 | Origin of neuronal-like receptors in Metazoa: cloning of a metabotropic glutamate/GABA-like receptor from the marine sponge Geodia cydonium显示文摘 | Sanja Perovic Anatoli Krasko I. Prokic Isabel M. Müller W. E. G. Müller | 1999 | Cell & Tissue Research1999，，2： | 1 |
| 17 | How do plants cope with oxidative stress in nature? A study on the dwarf bearded iris (Iris pumila)显示文摘 | Ana V Sanja M J Branka T | 2015 | Acta Physiologiae Plantarum2015，37，17： | 1 |
| 18 | Adding value to the oil cake as a waste from oil processing indus- try :production of lipase and protease by Candida uti- lisin solid state fermentation显示文摘 | Omar A S M Sanja G Milena : | 2012 | Applied Biochemis- try and Biotechnology2012，166，2： | 1 |
| 19 | Multi-objective genetic algorithms for scheduling of radiotherapy treatments for categorised cancer patients显示文摘 | Dobrila P Mohammad M Sanja P | 2011 | Expert Systems with Applications2011，38，6： | 1 |
| 20 | Characterization of traditional Istrian dry-cured ham by means of physical and chemical analyses and volatile compounds显示文摘 | NIVES M MARINKO P SANJA V | 2011 | Meat Science2011，88，4： | 1 |
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