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1篇 您的检索式:作者名="SHANG Youiun"
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1Infective viruses produced from full-length complementary DNA of swine vesicular disease viruses HK/70 strain显示文摘The full-length cDNA clone of swine vesicular disease virus HK/70 strain named pSVOK12 was constructed in order to study the antigenicity, replication, maturation and pathogenicity of swine vesicular disease virus. In vitro transcription RNA from pSVOK12 transfected IBRS-2 cells and the re- covered virus RNA were isolated and sequenced, then indirect hemagglutination test, indirect im- munofluorescence assays, eleectron microscope test, 50% tissue culture infecting dose (TCID50) assays and mouse virulence studies were performed to study the antigenicity and virulence of the recovered virus. The result showed that the infectious clones we ob- tained and the virus derived from pSVOK12 had the same biological properties as the parental strain HK/70. The full-length infectious cDNA clone, pSVOK12, will be very useful in studies of the anti- genicity, virulence, pathogenesis, maturation and replication of SVDV.ZHENG Haixue LIU Xiangtao SHANG Youiun WU Jinyan BAI Xingwen JIN Ye SUN Shiqi GUO Huichen TIAN Hong FENG Xia YIN Shuanghui GUO Jianhong CONG Guozheng LIU Zaixin CHANG Huiyun MA Junwu XIE Qingge 2006Chinese Science Bulletin2006,51,17:6
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