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您的检索式：作者名="SHE Zhen Ding"
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| 1 | Biocompatibility and Immunotoxicology of the Preclinical Implantation of a Collagen-based Artificial Dermal Regeneration Matrix显示文摘Objective Graft rejection, with the possibility of a violent immune response, may be severe and life threatening. Our aim was to thoroughly investigate the biocompatibility and immunotoxicology of collagen-based dermal matrix(DM) before assessment in clinical trials. Methods DM was subcutaneously implanted in BALB/c mice in two doses to induce a potential immune response. The spleen and lymph nodes were assessed for shape, cell number, cell phenotype via flow cytometry, cell activation via CCK8 kit, Annexin V kit, and Ki67 immunostaining. Serum samples were used to measure antibody concentration by enzyme-linked immunosorbent assay. Local inflammation was analyzed by histology and immunohistochemistry staining. Data analysis was performed by one-way ANOVA and non-parametric tests. Results Our data illustrate that the spleen and lymph node sizes were similar between the negative control mice and mice implanted with DM. However, in the high-dose DM(DM-H) group, the total cell populations in the spleen and lymph nodes, T cells and B cells in the spleen had slight increases in prophase, and the low-dose DM(DM-L) group did not display gross abnormities. Moreover, DM-H initiated moderate cell activation and proliferation in the early phase post-immunization, whereas DM-L did not. Neither DM-H nor DM-L implantation noticeably increased IgM and IgG serum concentrations. Examination of the local cellular response revealed only benign cell infiltration and TNF-α expression in slides of DM in the early phase. Conclusion Overall, DM-H may have induced a benign temporary acute immune response post-implantation, whereas DM-L had quite low immunogenicity. Thus, this DM can be regarded as a safe product. | WANG Wei ZHANG Lin SUN Lei SHE Zhen Ding TAN Rong Wei NIU Xu Feng | 2018 | Biomedical and Environmental Sciences2018，31，11： | 14 |
| 2 | Stable isotopic composition of riverine dissolved inorganic carbon of the Xijiang River Inner Estuary显示文摘为研究河流的溶解的无机的碳(DIC ) 的稳定的同位素的作文的时间空间的变化,我们在整个水疗院逻辑年执行了调查在期间它 δ
13 C 波浪交流的 DIC | JIAO Shulin TAO Zhen GAO Quanzhou LIU Kun SHE Jianwei DING Jian LIU Zufa | 2008 | Journal of Geographical Sciences2008，18，3： | 8 |
| 3 | Distribution of bone marrow stem cells in large porous polyester scaffolds显示文摘This work examined the optimal syringing depth during in vitro cell loading in order to even cell distribution after syringing a drop of cell suspension in cylinder poly(lactide-co-glycolide) (PLGA) porous scaffolds. The scaffolds of 10 mm height and 10 mm diameter were fabricated via room-temperature compression molding & particulate leaching technique based on spherical porogens. In vitro tests were employed for such examinations: a global observation of a cell-loaded scaffold stained by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) technique and a quantitative measurement of spatial distribution of cells after slicing the cell-loaded scaffolds into layers. It was found that an even distribution of cells was soon achieved only if the initial cell suspension was seeded on the layer that was below the top surface but above the middle of scaffolds. The availability of in vitro osteoblastic differentiation of rat bone marrow stem cells in such a kind of spherical-pore PLGA scaffolds was meanwhile confirmed. | WANG Zhen ZHANG Zheng ZHANG JunChuan SHE ZhenJue DING JianDong | 2009 | Chinese Science Bulletin2009，54，17： | 8 |
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