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| 1 | Phylogenetic relationships of five species of Dorippinae (Crustacea, Decapoda) revealed by 16S rDNA sequence analysis显示文摘A molecular phylogeny is presented for the subfamily Dorippinae (including 9 individuals, representing 5 species and 4 genera), based on the sequence data from 16S rRNA gene. Two-cluster test between lineages in these phylogenetic trees has been performed. On the basis of rate constancy, the rate of nucleotide substitutions of 16S rDNA sequence data is estimated as 0.27% per million years. The analysis strongly supports the recognition of the Dorippinae as a monophyletic subfamily. Phylogenetic tree indicates that the subfamily Dorippinae is divided into two main clades, and genus Dorippe appears basal in the subfamily, diverging from other species 36.6 Ma ago. It is also clear that the Heikea is closely related to the genus Neodorippe. The divergence time between them is 15.8 Ma. | FANYu LIXinzheng SONGLinsheng CAIZhonghua | 2004 | Acta Oceanologica Sinica2004，23，3： | 3 |
| 2 | cDNA cloning and mRNA expression of the translationally controlled tumor protein(TCTP)gene from Japanese sea perch(Lateolabrax japonicus)显示文摘A homologue of the lower vertebrates translationally controlled tumor protein (TCTP) was cloned from the marine fish Japanese sea perch (Lateolabrax japonicus) by the technology of homology cloning. The full-length cDNA sequence of the sea perch TCTP gene contained a 5’ untranslated region (UTR) of 47 bp, a 3’ UTR of 433 bp, and a putative open reading frame (ORF) of 510 bp encoding a polypeptide of 170 amino acids. The deduced amino acid sequence of the sea perch TCTP gene showed a high similarity to that of zebrafish, rohu, rabbit, chicken and human. Sequence analysis revealed there were a signature sequence of TCTP family, an N-glycosylation site, and five Casein kinase phosphorylation sites in the sea perch TCTP. The temporal expression of TCTP genes in healthy and lipopolysaccharide (LPS) challenged fishes was measured by semi-quantitative reverse transcription-PCR (RT-PCR). The results indicated that LPS could up-regulate the expression of sea perch TCTP in the examined tissues, including head-kidney, spleen and liver. | QIULihua SONGLinsheng WULongtao XUWei JIANGShigui | 2005 | Acta Oceanologica Sinica2005，24，2： | 3 |
| 3 | Molecular cloning and expression of interleukin Ibeta (IL-1β) from red seabream (Pagrus major)显示文摘The interleukin 1β (IL-1β) cDNA was cloned from the red seabream ( Pagrus major) by homology cloning strategy. A cDNA fragment was amplified by PCR using two degenerated primers, which were designed according to the conserved regions of other known IL-1β sequences, and elongated by 3' ends and 5' ends RACE PCR to get the full length coding sequence of red seabream IL-1β (RS IL-1β). The sequence contained 1252 nucleotides that included a 5' untranslated region (UTR) of 84 bp, a 3' UTR of 410 bp and an open reading frame (ORF) of 759 nucleotides which could be translated into a putative peptide of 253 amino acids with molecular weight of 28.6 kD and putative isoelectric point pl of 5.29. The deduced peptide contained two potential N-glycosylation sites and an identifiable IL1 family signature, but lacked the signal peptide and the clear ICE cut site, which were common in other nonmammalian IL-1β genes.The RS IL-1β had the highest homology with piscine IL-1β according to phylogenetic tree analysis. The transcript expression was detected in blood, brain, gill, heart, head kidney, kidney, liver, muscle and spleen in the pathogen challenged and healthy red seabream by RTPCR. Results showed that the RS IL-1β mRNA was constitutively expressed in most of the tissues both in stimulated and un-stimulated fish, and the expression could be enhanced by pathogen challenging. | CAIZhonghua SONGLinsheng GAOChunping WULongtao QIULihua | 2004 | Progress in Natural Science:Materials International2004，14，5： | 2 |
| 4 | Improvement of polyploidy induction in Eriocheir sinensis显示文摘An incubating temperature of 15 C is used to induce triploidy in Eriocheir sinensis through inhibition of the releaseof polar body II, and that of 18 C to induce tetraploidy through inhibition of the first cleavage. Flow cytometry isused to identify the ploidy in different developmental stages. For induction of triploidy in fertilized eggs in vitro, thehighest induction rate observed in blastula by cytochalasin B, 6-DMAP and KCl is 49.1%, 51.7% and 77.5%,respectively. In the KCl treatment of pregnant crabs with the fertilized eggs, the highest triploid induction rateobserved in the zoea is 85.3%. For induction of tetraploidy, the highest induction rate observed in the blastula bycytochaslasin B, 6-DMAP and KCl is 50.3%, 54.9% and 79.8% respectively. In the KCl treatment of pregnant crabswith the fertilized eggs, the highest induction rate in zoea is 27.3%. Through this study such difficulty as in vitroculture is overcome. Triploid zoea Eriocheir sinensis has been developed for the first time. The induction rate oftetraploid zoea has also been greatly improved. | CUIZhaoxia XIANGJianhai ZHOULinghua CAINaner SONGLinsheng | 2004 | Acta Oceanologica Sinica2004，23，4： | 0 |
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