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| 1 | A new simple model trust-region method with generalized Barzilai-Borwein parameter for large-scale optimization显示文摘In this paper, a new trust region method with simple model for solving large-scale unconstrained nonlinear optimization is proposed. By employing the generalized weak quasi-Newton equations, we derive several schemes to construct variants of scalar matrices as the Hessian approximation used in the trust region subproblem. Under some reasonable conditions, global convergence of the proposed algorithm is established in the trust region framework. The numerical experiments on solving the test problems with dimensions from 50 to 20,000 in the CUTEr library are reported to show efficiency of the algorithm. | ZHOU QunYan SUN WenYu ZHANG HongChao | 2016 | Science China Mathematics2016，59，11： | 4 |
| 2 | Defensive deception framework against reconnaissance attacks in the cloud with deep reinforcement learning显示文摘Implementing defensive deception in the cloud is promising to proactively counter reconnaissance attack. This technique presents decoys to camouflage cloud assets and distracts attack resource. However,the major challenge is to develop an effective deception strategy to orchestrate digital decoys. To address this issue, we propose a deep reinforcement learning(DRL)-based defensive deception framework. First,we formulate a utility function, which mathematically models underlying threats associated with common vulnerabilities among virtual machines in the cloud. Then, we customize training interfaces and the neural networks for a DRL agent. The reward function reflects the effectiveness of asset concealment and the waste of attack resources, referring to a comprehensive defense goal. Finally, the well-trained DRL agent generates the optimal defense strategy. It specifies a more granular deception strategy than existing proposals. Simulation results show that the proposed framework leads to a 7.87% average advantage in realizing the comprehensive defense goal. Moreover, it can stably improve the concealment degree of cloud assets up to 20.58%, and increase the attack cost up to 40.40%. This study shows that it is promising to improve cloud security with deception defense and artificial intelligence techniques. | Huanruo LI Yunfei GUO Shumin HUO Hongchao HU Penghao SUN | 2022 | Science China(Information Sciences)2022，65，7： | 3 |
| 3 | Influence of Preparation Conditions on the Catalytic Performance of MoN_x/SBA-15 for Ammonia Decomposition显示文摘准备条件的影响(例如 H2-N2 比率,最后的氮化温度) 在为氨的 MoNx/SBA-15 催化剂的表演上,分解被调查。有 H2-N2 比率的催化活的变化可以被归因于表面作文和活跃部件的粒子大小的变化。氮化温度的变化导致改变作文的铝氮化物领域的形成,它为在他们关于氨分解的催化表演的差别负责。在 923 K,氨能完全用 15800 ml/h 被分解 ? GHSVNH3 的¤ g 猫,哪个为氨的催化分解的表演高效。给词调音:准备条件;MoNx/SBA-15;氨分解; | Hongchao Liu Hua Wang Jianghan Shen Ying Sun Zhongmin Liu | 2006 | Journal of Natural Gas Chemistry2006，15，3： | 3 |
| 4 | Methylation status of WWOX gene promoter CpG islands in epithelial ovariancancer and its clinical significance显示文摘 | Hongchao Yan Jieyun Sun | 2013 | Biomedical Reports2013，，3： | 1 |
| 5 | Stability condition of finite difference solution for viscoelastic wave equations显示文摘The stability problem is a very important aspect in seismic wave numerical modeling.Based on the theory of seismic waves and constitutive equations of viscoelastic models,the stability problems of finite difference scheme for Kelvin-Voigt and Maxwell models with rectangular grids are analyzed.Expressions of stability conditions with arbitrary spatial accuracies for two viscoelastic models are derived.With approximation of quality factor Q≥5,simplified expressions are developed and some numerical models are given to verify the validity of the corresponding theoretical results.Then this paper summarizes the influences of seismic wave velocity,frequency,size of grid and difference coefficients,as well as quality factor on stability condition.Finally the prerequisite conditions of the simplified stability equations are given with error analysis. | Chengyu Sun Yunfei Xiao Xingyao Yin Hongchao Peng | 2009 | Earthquake Science2009，22，5： | 1 |
| 6 | Urban spatial structure analysis:quantitative identification of urban social functions using building footprints显示文摘Analysis of urban spatial structures is an effective way to explain and solve increasingly serious urban problems.However,many of the existing methods are limited because of data quality and availability,and usually yield inaccurate results due to the unclear description of urban social functions.In this paper,we present an investigation on urban social function based spatial structure analysis using building footprint data.An improved turning function(TF)algorithm and a selforganizing clustering method are presented to generate the variable area units(VAUs)of high-homogeneity from building footprints as the basic research units.Based on the generated VAUs,five spatial metrics are then developed for measuring the morphological characteristics and the spatial distribution patterns of buildings in an urban block.Within these spatial metrics,three models are formulated for calculating the social function likelihoods of each urban block to describe mixed social functions in an urban block,quantitatively.Consequently,the urban structures can be clearly observed by an analysis of the spatial distribution patterns,the development trends,and the hierarchy of different social functions.The results of a case study conducted for Munich validate the effectiveness of the proposed method. | Zhiyao ZHAO Xianwei ZHENG Hongchao FAN Mengqi SUN | 2021 | Frontiers of Earth Science2021，15，3： | 1 |
| 7 | Effects of arginine replacement with L-citrulline on the arginine/nitric oxide metabolism in chickens:An animal model without urea cycle显示文摘Background This study examined the efficacy of L-citrulline supplementation on the arginine/nitric oxide metabolism,and intestinal functions of broilers during arginine deficiency.A total of 288 day-old Arbor Acre broilers were randomly assigned to either an arginine deficient basal diet(NC diet),NC diet+0.50%L-arginine(PC diet),or NC diet+0.50%L-citrulline(NCL diet).Production performance was recorded,and at 21 days old,chickens were euthanized for tissue collection.Results The dietary treatments did not affect the growth performance of broilers(P>0.05),although NC diet increased the plasma alanine aminotransferase,urate,and several amino acids,except arginine(P<0.05).In contrast,NCL diet elevated the arginine and ornithine concentration higher than NC diet,and it increased the plasma citrulline greater than the PC diet(P<0.05).The nitric oxide concentration in the kidney and liver tissues,along with the plasma and liver e NOS activities were promoted by NCL diet higher than PC diet(P<0.05).In the liver,the activities of arginase 1,ASS,and ASL,as well as,the gene expression of i NOS and OTC were induced by PC diet greater than NC diet(P<0.05).In the kidney,the arginase 1,ASS and ASL enzymes were also increased by PC diet significantly higher than the NC and NCL diets.Comparatively,the kidney had higher abundance of n NOS,ASS,ARG2,and OTC genes than the liver tissue(P<0.05).In addition,NCL diet upregulated(P<0.05)the m RNA expression of intestinal nutrient transporters(EAAT3 and PEPT1),tight junction proteins(Claudin 1 and Occludin),and intestinal mucosal defense(MUC2 and p Ig R).The intestinal morphology revealed that both PC and NCL diets improved(P<0.05)the ileal VH/CD ratio and the jejunal VH and VH/CD ratio compared to the NC fed broilers.Conclusion This study revealed that NCL diet supported arginine metabolism,nitric oxide synthesis,and promoted the intestinal function of broilers.Thus,L-citrulline may serve as a partial arginine replacement in broiler’s diet without detrimental impacts on the performance,arginine metabolism and gut health of chickens. | Victoria Anthony Uyanga Lijing Sun Yu Liu Meiming Zhang Jingpeng Zhao Xiaojuan Wang Hongchao Jiao Okanlawon M.Onagbesan Hai Lin | 2023 | Journal of Animal Science and Biotechnology2023，14，3： | 1 |
| 8 | Design of a prompt-gamma neutron activation analysis system on China Advanced Research Reactor显示文摘In this paper,computational methods are used to optimize the design of a prompt-gamma neutron activation analysis(PGNAA) system on China Advanced Research Reactor(CARR).Approaches are adopted for obtaining accurate neutron beam parameter and saving the computing time.For the radiation shielding design,the optimizing factors include the cost,weight,volume,machining convenience and background radiation at the detector position.Low background spectrum and high sensitivity are expected.The simulation results,and experiences from international PGNAA community,were used in the design of the CARR PGNAA system. | SUN Hongchao NI Bangfa XIAO Caijin ZHANG Guiying LIU Cunxiong HUANG Jinfeng | 2011 | Nuclear Science and Techniques2011，22，5： | 0 |
| 9 | Targeting myeloid-derived suppressor cells for cancer therapy显示文摘The emergence and clinical application of immunotherapy is considered a promising breakthrough in cancer treatment.According to the literature,immune checkpoint blockade(ICB)has achieved positive clinical responses in different cancer types,although its clinical efficacy remains limited in some patients.The main obstacle to inducing effective antitumor immune responses with ICB is the development of an immunosuppressive tumor microenvironment.Myeloid-derived suppressor cells(MDSCs),as major immune cells that mediate tumor immunosuppression,are intimately involved in regulating the resistance of cancer patients to ICB therapy and to clinical cancer staging and prognosis.Therefore,a combined treatment strategy using MDSC inhibitors and ICB has been proposed and continually improved.This article discusses the immunosuppressive mechanism,clinical significance,and visualization methods of MDSCs.More importantly,it describes current research progress on compounds targeting MDSCs to enhance the antitumor efficacy of ICB. | Hongchao Tang Hao Li Zhijun Sun | 2021 | Cancer Biology & Medicine2021，18，4： | 0 |
| 10 | Confinement of Fe atoms between MoS_(2) interlayers drives phase transition for improved reactivity in Fenton-like reactions显示文摘Phase manipulation of MoS_(2) from thermodynamically stable 2H phase to the unstable but more reactive 1T phase represents a crucial strategy for improving the reactivity in many reactions.The widely adopted wet chemistry approach uses intercalating entities especially alkali metal ions to achieve the phase transition;however,these entities are normally inert for the target reaction.Here,we describe the first use of iron atoms for the intercalation of 2H-MoS_(2) layers,driving the partial transition from 2H to 1T phase.Interestingly,in the peroxymonosulfate(PMS)-based Fenton-like reactions,the interlayered confinement of Fe atoms not only activates the inert basal plane,but also adds more reactive Fe sites for the formation of metal-PMS complex as primary reactive species for pollutant removal.In the degradation of a model pollutant carbamazepine(CBZ),the Fe-intercalated MoS_(2) exhibits a first order rate constant 13.3 times higher than 2H-MoS_(2).This strategy is a new direction for manipulating the phase composition and boosting the catalytic reactivity of MoS_(2)-based catalysts in various scenarios,including environmental remediation and energy applications. | Yibing Sun Yu Zhou Hongchao Li Chuan Wang Xuan Zhang Qian Ma Yingchun Cheng Jieshu Qian Bingcai Pan | 2024 | Nano Research2024，17，3： | 0 |
| 11 | Macrophage LMO7 deficiency facilitates inflammatory injury via metabolic-epigenetic reprogramming显示文摘Inflammatory bowel disease(IBD)is a formidable disease due to its complex pathogenesis.Macrophages,as a major immune cell population in IBD,are crucial for gut homeostasis.However,it is still unveiled how macrophages modulate IBD.Here,we found that LIM domain only 7(LMO7)was downregulated in pro-inflammatory macrophages,and that LMO7 directly degraded 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3(PFKFB3)through K48-mediated ubiquitination in macrophages.As an enzyme that regulates glycolysis,PFKFB3 degradation led to the glycolytic process inhibition in macrophages,which in turn inhibited macrophage activation and ultimately attenuated murine colitis.Moreover,we demonstrated that PFKFB3 was required for histone demethylase Jumonji domaincontaining protein 3(JMJD3)expression,thereby inhibiting the protein level of trimethylation of histone H3 on lysine 27(H3K27me3).Overall,our results indicated the LMO7/PFKFB3/JMJD3 axis is essential for modulating macrophage function and IBD pathogenesis.Targeting LMO7 or macrophage metabolism could potentially be an effective strategy for treating inflammatory diseases. | Shixin Duan Xinyi Lou Shiyi Chen Hongchao Jiang Dongxin Chen Rui Yin Mengkai Li Yuseng Gou Wenjuan Zhao Lei Sun Feng Qian | 2023 | Acta Pharmaceutica Sinica B2023，13，12： | 0 |
| 12 | Phylogeny of SARS-CoV as inferred from complete genome comparison显示文摘SARS-CoV, as the pathogeny of severe acute respiratory syndrome (SARS), is a mystery that the origin of the virus is still unknown even a few isolates of the virus were completely sequenced. To explore the genesis of SARS-CoV, the FDOD method previously developed by us was applied to comparing complete genomes from 12 SARS-CoV isolates to those from 12 previously identified coronaviruses and an unrooted phylogenetic tree was constructed. Our results show that all SARS-CoV isolates were clustered into a clique and previously identified coronaviruses formed the other clique. Meanwhile, the three groups of coronaviruses depart from each other clearly in our tree that is consistent with the results of prevenient papers. Differently, from the topology of the phylogenetic tree we found that SARS-CoV is more close to group 1 within genus coronavirus. The topology map also shows that the 12 SARS-CoV isolates may be divided into two groups determined by the association with the SARS-CoV from the Hotel M in Hong Kong that may give some information about the infectious relationship of the SARS. | QI Zhen HU Yu LI Wei CHEN Yanjun ZHANG Zhihua SUN Shiwei LU Hongchao ZHANG Jingfen BU Dongbo LING Lunjiang CHEN Runsheng | 2003 | Chinese Science Bulletin2003，48，12： | 0 |
| 13 | The optimal dietary arginine level of laying hens fed with low-protein diets显示文摘Background:Arginine(Arg)is an essential amino acid(EAA)in poultry,an important substrate for protein synthesis and a precursor of several molecules.Supplementation of EAAs with low protein(LP)diet increases the utilization efficiency of dietary crude protein(CP).However,if the EAA requirement is changed in hens fed a LP diet remains to be elucidated.The aim of the present study was to evaluate the optimal level of dietary Arg in the LP diet of hens.A total of 1350 Hy-Line Brown laying hens were randomly allocated to six dietary treatments:a basal diet(16%CP,positive control),or an isoenergetic LP diet(14%CP,0.80%Arg)supplemented 0,0.05%,0.10%,0.15%,and 0.20%L-Arg,corresponding to 0.80%,0.85%,0.90%,0.95%and 1.00%dietary Arg,respectively.Results:The feed efficiency was decreased(P<0.05)by 0.80%and 1.00%Arg-LP diets,compared to control.Within LP diets,dietary Arg level had significant quadratic effects(P<0.05)on laying rate,egg mass,and feed efficiency.Compared to control,the plasma CAT activity or T-AOC content were decreased by 0.80%(P<0.001).However,the hens offered 0.85%and 0.90%Arg-LP diets had higher CAT activity(P<0.001)than 0.80%Arg-LP diet.In contrast,1.00%Arg-LP group had the highest MDA and the lowest T-AOC content in plasma,liver,duodenal and jejunal mucosa(P<0.05).Compared to control,the villus height was decreased by 0.80%,0.95%and 1.00%Arg-LP diets,while the villus height to crypt depth(V/C)ratio was reduced by 0.95%and 1.00%Arg-LP diets in duodenum.Conclusion:The result demonstrates that LP diet(14%CP)deficient in Arg(0.80%Arg)result in augmented oxidative damage and impaired development of intestinal mucosa.According to the quadratic broken-line regression model,the optimal dietary arginine levels for Hy-Line Brown laying hens fed with low protein diet(14%CP)aged 33 to 40 weeks are 0.85%,0.86%,and 0.86%to obtained the maximum laying rate,egg mass,and feed efficiency,respectively. | Mingfa Sun Ning Ma Hui Liu Yu Liu Yunlei Zhou Jingpeng Zhao Xiaojuan Wang Haifang Li Baishun Ma Hongchao Jiao Hai Lin | 2022 | Journal of Animal Science and Biotechnology2022，13，6： | 0 |
| 14 | AgAuSe quantum dots with absolute photoluminescence quantum yield of 87.2%:The effect of capping ligand chain length显示文摘Surface ligands of colloidal quantum dots(QDs)have a profound influence on their surface states,which has been verified in the studies of the effect of ligand head groups on the photoluminescence(PL)properties of QDs.However,the investigation of the ligand chain length is limited.Here,we systematically explored the effect of chain length on the Ag_(2)Se QDs by selecting three ligands,1-octanethiol(OTT),1-dodecanethiol(DDT),and 1-hexadecanethiol(HDT),with diverse chain lengths.We found that the PL intensity of Ag_(2)Se QDs increased with the decrease of the ligand chain length due to the enhanced passivation of surface defects emerging from the robust QD-ligand interface binding affinity and the weaker hydrophobic chain–chain interaction.Subsequently,AgAuSe QDs terminated with OTT were obtained by alloying parent OTT-Ag_(2)Se QDs with Au precursor with a record absolute PL quantum yield(PLQY)of 87.2%at 970 nm,facilitating ultrasensitive in vivo angiography imaging in a nude mouse model.We expect that our finding of the important role of the ligand chain length on the optical properties of QDs will be suggestive to the design and synthesis of high-quality QDs,and also look forward to the clinical applications of the ultra-bright AgAuSe QDs. | Ziqiang Sun Cheng Liu Hongchao Yang Xiaohu Yang Yejun Zhang Hongzhen Lin Youyong Li Qiangbin Wang | 2022 | Nano Research2022，15，9： | 0 |
| 15 | Si_(3)N_(4)nanowires@pyrolytic carbon nanolayers coupled withhydroxyapatite nanosheets as reinforcement for carbon matrixcomposites with boosting mechanical and friction properties显示文摘Extensive attention has been drawn to the development of carbon-matrix composites for application in the aerospace and military industry,where a combination of high mechanical strength and excellent frictional properties are required.Herein,carbon-matrix composites reinforced by Si_(3)N_(4)nanowires@pyrolytic carbon nanolayers(Si_(3)N_(4nws)@PyCnls)coupled with hydroxyapatite nanosheets is reported.The Si_(3)N_(4nws)@PyCnls(SP)with coaxial structure could increase the surface roughness of Si_(3)N_(4nws)and promote the stress transfer to the carbon matrix,whereas the porous hydroxyapatite nanosheets favor the infiltration of the carbon matrix and promote the interfacial bonding between the SP and carbon matrix.The carbon matrix composites reinforced by SP coupled with hydroxyapatite nanosheets(Si_(3)N_(4nws)@PyCnls-HA-C)exhibit excellent mechanical strength.Compare with the conventional Si_(3)N_(4nws)reinforced carbon composites,Si_(3)N_(4nws)@PyCnls-HA-C(SPHC)have 162%and 249%improvement in flexural strength and elastic modulus,respectively.Moreover,the friction coefficient and wear rate decreased by 53%and 23%,respectively.This study provides a co-reinforcement strategy generated by SP coupled with hydroxyapatite nanosheets for effective improvement of mechanical and frictional properties of carbon matrix composites that are used for aerospace and military industry applications. | Lina Sun Leilei Zhang Xuemin Yin Yeye Liu Yao Guo Hongchao Sheng Xianghui Hou | 2023 | Journal of Materiomics2023，9，1： | 0 |
| 16 | Distribution Properties of Internal Air Voids in Ultrathin Asphalt Friction Course显示文摘The distribution characteristics of air voids in ultrathin asphalt friction course(UAFC) samples with different gradations and compaction methods were statistically analyzed using X-ray computed tomography(CT) and image analysis techniques. Based on the results, compared with the AC-5 sample, the OGFC-5mixture has a higher air void ratio, a larger air void size and a greater number of air voids, with the distribution of internal air voids being more uniform and their shapes being more rounded. The two-parameter Weibull function was applied to fit the gradation of air voids. The fitting results is good, and the function parameters are sensitive to changes in both mineral gradation and compaction method. Moreover, two homogeneity indices were proposed to evaluate the compaction uniformity of UAFC samples. Compared with the Marshall method,the SGC method is more conducive to improve the compaction uniformity of UAFC samples. The compaction method significantly influences the air void distribution characteristics and compaction uniformity of AC-5sample, but has a less significant impact on OGFC-5 sample. The experimental results in the study provides a solid foundation for further explorations on the internal structure and mixture design of UAFC. | 林宏伟 杜晓博 ZHONG Changyu WU Ping LIU Wenchang SUN Mutian ZHANG Hongchao | 2023 | Journal of Wuhan University of Technology(Materials Science)2023，38，3： | 0 |
| 17 | Land Use/Cover Change and Its Policy Implications in Typical Agriculture-forest Ecotone of Central Jilin Province,China显示文摘During the 21st century,policies toward agriculture,forestry,and urbanization have emerged to ensure food security,ecological restoration,and human well-being by managing land in Northeast China.However,the integrated effects and relationships of various policies are still not well understood.This study observed the land use land cover changes in Central Jilin from 2000 to 2019 and,by considering policy involvement,aimed to understand the effects and trade-offs of policies.Results showed that the cropland,including dryland and rice paddy,and the forest,including coniferous forest and deciduous forest,are dominant land types in Central Jilin.During 2000–2019,the land changed diversely,of which the main changes were the expanded dryland(+0.43 million ha),the increased deciduous forest(+22 million ha),the decreased coniferous forest(–0.08 million ha),and the expanded urban settlement(+0.04 million ha).With these changes,despite the unit grain yield showing a rising trend,the yield contribution of Central Jilin to the national total decreased.The poor cultivating structure made for the cropland expansion and reduced the implementation space of environmental restoration projects such as the Grain to Green.Thus,in Central Jilin that transits from the agri-food production zone to the eco-regulation zone,environmental projects coexisted in a trade-off manner with agricultural policies that aim to liberate agricultural productivity.In the key urban agglomerations of Central Jilin,the increase in the proportion of green space improved the thermal environment and carbon balance.The gross domestic product of the large city and its local proportion also rose.These improvements benefited from the promotion of development policies and urbanization policies at key time points.In the future,it is necessary to coordinate agricultural policies and environmental projects and promote the progress of small-and medium-sized cities to ensure the equality of regional development.This study has implications for making decisions to revitalize Northeast China and researchers who inform decisions. | DONG Yulin REN Zhibin FU Yao YANG Ran SUN Hongchao HE Xingyuan | 2021 | Chinese Geographical Science2021，31，2： | 0 |
| 18 | Double[3+2] cycloaddition of nitrile oxides with allenoates:Synthesis of spirobidihydroisoxazoles显示文摘The double [3 + 2] cycloaddition of allenoates with nitrile oxides is presented. The reaction worked well under mild reaction conditions to give the spirobidihydroisoxazole in moderate to excellent yields with excellent diastereoselectivities. The two dihydroisoxazole rings have been formed via a sequential double [3 + 2] cycloaddition. | Wei Sun Feng Jiang Honglei Liu Xing Gao Hao Jia Cheng Zhang Hongchao Guo | 2019 | Chinese Chemical Letters2019，30，2： | 0 |
| 19 | Damage Mechanism of Ultra-thin Asphalt Overlay(UTAO) based on Discrete Element Method显示文摘Aiming to analyze the damage mechanism of UTAO from the perspective of meso-mechanical mechanism using discrete element method(DEM),we conducted study of diseases problems of UTAO in several provinces in China,and found that aggregate spalling was one of the main disease types of UTAO.A discrete element model of UTAO pavement structure was constructed to explore the meso-mechanical mechanism of UTAO damage under the influence of layer thickness,gradation,and bonding modulus.The experimental results show that,as the thickness of UTAO decreasing,the maximum value and the mean value of the contact force between all aggregate particles gradually increase,which leads to aggregates more prone to spalling.Compared with OGFC-5 UTAO,AC-5 UTAO presents smaller maximum and average values of all contact forces,and the loading pressure in AC-5 UTAO is fully diffused in the lateral direction.In addition,the increment of pavement modulus strengthens the overall force of aggregate particles inside UTAO,resulting in aggregate particles peeling off more easily.The increase of bonding modulus changes the position where the maximum value of the tangential force appears,whereas has no effect on the normal force. | 杜晓博 GAO Liang RAO Faqiang 林宏伟 ZHANG Hongchao SUN Mutian XU Xiuchen | 2024 | Journal of Wuhan University of Technology(Materials Science)2024，39，2： | 0 |
| 20 | L-Arginine/nitric oxide regulates skeletal muscle development via muscle fibre-specific nitric oxide/mTOR pathway in chickens显示文摘L-Arginine (L-Arg), the precursor of nitric oxide (NO), plays an important role in muscle function. Fasttwitchglycolytic fibres are more susceptible to age-related atrophy than slow-twitch oxidative fibres.The effect of L-Arg/NO on protein metabolism of fast- and slow-twitch muscle fibres was evaluated inchickens. In Exp. 1, 48 chicks at 1 day old were divided into 4 groups of 12 birds and subjected to 4treatments: basal diet without supplementation or supplemented with 1% L-Arg, and water supplementedwith or without L-nitro-arginine methyl ester (L-NAME, 18.5 mM). In Exp. 2, 48 chicks weredivided into 4 groups of 12 birds fed with the basal diet and subjected to the following treatments: tapwater (control), tap water supplemented with L-NAME (18.5 mM), or molsidomine (MS, 0.1 mM), or18.5 mM L-NAME t 0.1 mM MS (NAMS). The regulatory effect of L-Arg/NO was further investigatedin vitro with myoblasts obtained from chicken embryo pectoralis major (PM) and biceps femoris (BF).In vivo, dietary L-Arg supplementation increased breast (t14.94%, P < 0.05) and thigh muscle mass(t23.40%, P < 0.05);whereas, MS treatment had no detectable influence. However, L-NAME treatmentblocked the beneficial influence of L-Arg on muscle development. L-Arg decreased (P < 0.05) proteinsynthesis rate, phosphorylated mTOR and ribosomal protein S6 kinase beta-1 (p70S6K) levels in breastmuscle, which was recovered by L-NAME treatment. In vitro, L-Arg or sodium nitroprusside (SNP)reduced protein synthesis rate, suppressed phosphorylated mTOR/p70S6K and decreased atrogin-1 andmuscle RING finger 1 (MuRF1) in myoblasts from PM muscle (P < 0.05). L-NAME abolished the inhibitoryeffect of L-Arg on protein synthesis and the mTOR/p70S6K pathway. However, myoblasts from BF muscleshowed the weak influence. Moreover, blocking the mTOR/p70S6K pathway with rapamycin suppressedprotein synthesis of the 2 types of myoblasts;whereas, the protein expression of atrogin-1 and MuRF1levels were restricted only in myoblasts from PM muscle. In conclusion, L-Arg/NO/mTOR/p70S6Kpathway enhances protein accumulation and muscle development in fast-twitch glycolytic muscle inchickens. L-Arg/NO regulates protein turnover in a muscle fibre specific way, which highlights the potentialclinical application in fast-twitch glycolytic muscle fibres. | Ruxia Wang Kelin Li Li Sun Hongchao Jiao Yunlei Zhou Haifang Li Xiaojuan Wang Jingpeng Zhao Hai Lin | 2022 | Animal Nutrition2022，，3： | 0 |
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