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| 1 | Developmental changes in contraction of gastric smooth muscle cells in rats correlate with their differences in RhoA/ROCK pathway显示文摘Previous research has shown that smooth muscle of the stomach undergoes developmental changes in the intracellular regulatory mechanism responsible for the contractile process. Whether these developmental changes relate to differences in the expression and/or activity of the key enzymes regulating smooth muscle contraction has not been previously evaluated. Therefore, we aimed to examine the expression and activation of the small monomeric G protein 'Rho A' and Rho kinase(ROCK) as well as their correlation with the contraction of gastric smooth muscle cells(GSMCs) in newborn vs. adult rats. Freshly isolated single GSMCs from Sprague-Dawley rats at 1 week(newborn) and 3 months(adult) of age were used in the study. Protein and m RNA expression levels of both ROCK2 and total Rho A were higher in adult compared to newborn rats. Moreover, acetylcholine(ACh)-induced contractions of GSMCs in adult rats were significantly higher than that in newborn animals. Meanwhile,ROCK and Rho activation was higher in adult stomach cells compared to newborn ones. Pretreatment of GSMCs with Y-27632, the ROCK inhibitor, significantly reduced ACh-induced contraction in both groups of cells and greatly abolished contractile differences. In conclusion, our results indicate that Rho A/ROCK pathway and contraction of stomach muscle cells are under developmental regulation. | Othman Al-Shboul Mahmoud Alfaqih Ayman Mustafa Ahmed Al-Dwairi Mohammad Alqudah Saja Haifawi Mohanad Alebbini Mohammad Al-Zaareer Khobaib Alahmar | 2019 | The Journal of Biomedical Research2019，33，5： | 2 |
| 2 | Specifc inflammatory osteoclast precursors induced during chronic inflammation give rise to highly active osteoclasts associated with inflammatory bone loss显示文摘Elevated osteoclast(OC)activity is a major contributor to inflammatory bone loss(IBL)during chronic inflammatory diseases.However,the specific OC precursors(OCPs)responding to inflammatory cues and the underlying mechanisms leading to IBL are poorly understood.We identified two distinct OCP subsets:Ly6C^(hi)CD11b^(hi) inflammatory OCPs(iOCPs)induced during chronic inflammation,and homeostatic Ly6C^(hi)CD11b^(lo)OCPs(hOCPs)which remained unchanged.Functional and proteomic characterization revealed that while iOCPs were rare and displayed low osteoclastogenic potential under normal conditions,they expanded during chronic inflammation and generated OCs with enhanced activity.In contrast,hOCPs were abundant and manifested high osteoclastogenic potential under normal conditions but generated OCs with low activity and were unresponsive to the inflammatory environment.Osteoclasts derived from iOCPs expressed higher levels of resorptive and metabolic proteins than those generated from hOCPs,highlighting that different osteoclast populations are formed by distinct precursors.We further identified the TNF-αand S100A8/A9 proteins as key regulators that control the iOCP response during chronic inflammation.Furthermore,we demonstrated that the response of iOCPs but not that of hOCPs was abrogated in tnf-α^(-/-)mice,in correlation with attenuated IBL.Our findings suggest a central role for iOCPs in IBL induction.iOCPs can serve as potential biomarkers for IBL detection and possibly as new therapeutic targets to combat IBL in a wide range of inflammatory conditions. | Yaron Meirow Milena Jovanovic Yuval Zur Juliana Habib Daniele Filippo Colombo Nira Twaik Hadas Ashkenazi-Preiser Kerem Ben-Meir Ivan Mikula Jr. Or Reuven Guy Kariv Leonor Daniel Saja Baraghithy Yehuda Klein Jeroen Krijgsveld Noam Levaot Michal Baniyash | 2022 | Bone Research2022，10，3： | 2 |
| 3 | An onroad motor vehicle emissions inventory for Denver: an efficient alternative to modeling 显示文摘 | Saja S Pokharel Gary A Bishop Donald H Sted man | 2002 | Atomospheric Environment2002，36，33： | 1 |
| 4 | Functionalized graphene sheet filled silicone foam nanocomposites 显示文摘 | AngelaRodriguez Perez M de Saja J A AngelaLopez Manchado M | 2008 | Journal of Materials Chemistry2008，18，19： | 1 |
| 5 | Constrained crystallization and activity of filler in surface modified talc polypropylene composites显示文摘 | Alonso M Velasco J I de Saja J A | 1997 | Eur Poly J1997，33，3： | 1 |
| 6 | Anti-inflammatory effect of curcu- rain involves downregulation of MMP-9 in blood mononuclear cells 显示文摘 | Saja K Babu MS Karuagaran D et at | 2007 | Int Immunopharmaco12007，7，13： | 1 |
| 7 | Crystallization behavior of polypropylene filled with surface - modifled talc显示文摘 | VELASCO J I DE SAJA J A MARTINEZ A B | 1996 | J Appl Polym Sci1996，61，： | 1 |
| 8 | Foaming behavior, cellular structure and physical properties of poly- benzoxazine foams显示文摘 | Ardanuy M Rodriguez-P6rez M ~ de Saja J A | 2012 | Polymers for Advanced Technologies2012，23，5： | 1 |
| 9 | Strategies to improve the mechanical properties of starchbased materials:Plasticization and natural fibers reinforcement显示文摘 | Lopez-Gil A Rodriguez-Perez M A De Saja J A | 2014 | Polímeros Ciênciae Tecnologia2014，，： | 1 |
| 10 | Langmuir-blodgett mixed films of titanyl( VI ) pthalocyanine and arachidic acid: Molecular orientation and film structure显示文摘 | CANO T D AROCA R SAJA D J A RODRIGUEZ-MENDEZ M L | 2003 | Langmuir2003，19，： | 1 |
| 11 | Array of sensors based on conducting polymers for the quality control of the aroma of the virgin olive oil显示文摘 | Guadarrama A Rodriguez-Mendez M L De Saja J A | 2000 | Sensors and Actuators B:Chemical2000，69，3： | 1 |
| 12 | Tensile forces stimulate vascular remodeling and epidermal cell proliferation in living skin 显示文摘 | Giorgio P Perry L Saja S | 2007 | Ann Surg2007，246，5： | 1 |
| 13 | Analysis of saffron volatile fraction by TD–GC–MS and e-nose显示文摘 | Manuel Carmona Javier Martínez Amaya Zalacain Ma Luz Rodríguez-Méndez José Antonio Saja Gonzalo Luis Alonso | 2006 | European Food Research and Technology2006，，1： | 1 |
| 14 | Constrained crystallization and activity of filler in surface modified talc polypropylene composites显示文摘 | ALONSO M VELASCO J I SAJA J A | 1997 | J Eur Poly1997，33，： | 1 |
| 15 | Dy- namic Meehanieal Analysis of Injection-moulded Discs of Polypropylene and Untreated and Silane-Treated Tale-Filled Polypropylene composites 显示文摘 | DIEZ Gutierrez S RODR1GUEZ Ma DE Saja Ja | 1999 | Polymer1999，40，19： | 1 |
| 16 | Modified carbon paste electrodes for discrimination of vegetable oils 显示文摘 | Apetrei C Rodriguez-Mendez M L De Saja J A | 2005 | Sensors and Actuators B2005，，111112： | 1 |
| 17 | Extinction Coefficient of Polyolefin Foams显示文摘 | R.A.Campo Arnaiz M.A.Rodriguez Perez B.Calvo J.A.De Saja | | 0，，： | 1 |
| 18 | Langmuir- Blodgett film and carbon paste electrodes based on phthalocyanines as sensing units for taste显示文摘 | Arrieta A Rodriguez- Mendez M L de Saja J A | 2003 | Sensors and Actuators B2003，95，： | 1 |
| 19 | Evaluation of oxygen exposure levels and polyphenolic content of red wines using an electronic panel formed by an electronic nose and an electronic tongue显示文摘 | M.L. Rodriguez-Mendez C. Apetrei M. Gay C. Medina-Plaza J.A. de Saja S. Vidal O. Aagaard M. Ugliano J. Wirth V. Cheynier | 2014 | Food Chemistry2014，，： | 1 |
| 20 | Dynamic Mechanical Analysis of Injection- moulded Discs of Polypropylene and Untreated and Silane- Treated Talc- Filled Polypropylene composites显示文摘 | Diez-Gutierrez s Rodriguez Ma De Saja Ja | 1999 | Polymer1999，40，19： | 1 |
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