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| 1 | Pyrrolidine dithiocarbamate reduces ischemia-reperfusion injury of the small intestine显示文摘AIM: To evaluate whether pyrrolidine dithiocarbamate (PDTC), an enhancer of HO production, attenuates intestinal IR injury.METHODS: Eighteen male rats were randomly allocated into three groups: (a) sham; (b) IR, consisting of 30 min of intestinal ischemia, followed by 2-h period of reperfusion; and (c) PDTC treatment before IR. Intestinal microvascular perfusion (IMP) was monitored continuously by laser Doppler fiowmetry. At the end of the reperfusion, serum samples for lactate dehydrogenase (LDH) levels and biopsies of ileum were obtained. HO activity in the ileum was assessed at the end of the reperfusion period.RESULTS: At the end of the reperfusion in the IR group,IMP recovered partially to 42.5% of baseline (P<0.05vs sham), whereas PDTC improved IMP to 67.3% of baseline (P<0.01 vs IR). There was a twofold increase in HO activity in PDTC group (2 062.66±106.11) as compared to IR (842.3±85.12) (P<0.001). LDH was significantly reduced (P<0.001) in PDTC group (585.6±102.4)as compared to IR group (1 973.8±306.5). Histological examination showed that the ileal mucosa was significantly less injured in PDTC group as compared with IR group.CONCLUSION: Our study demonstrates that PDTC improves the IMP and attenuates IR injury of the intestine possibly via HO production. Additional studies are warranted to evaluate the clinical efficacy of PDTC in the prevention of IR injury of the small intestine. | Ismail H Mallick Wen-Xuan Yang Marc C Winslet Alexander M Seifalian | 2005 | World Journal of Gastroenterology2005，11，46： | 8 |
| 2 | Advances in regenerative therapies for spinal cord injury:a biomaterials approach显示文摘Spinal cord injury results in the permanent loss of function,causing enormous personal,social and economic problems.Even though neural regeneration has been proven to be a natural mechanism,central nervous system repair mechanisms are ineffective due to the imbalance of the inhibitory and excitatory factors implicated in neuroregeneration.Therefore,there is growing research interest on discovering a novel therapeutic strategy for effective spinal cord injury repair.To this direction,cell-based delivery strategies,biomolecule delivery strategies as well as scaffold-based therapeutic strategies have been developed with a tendency to seek for the answer to a combinatorial approach of all the above.Here we review the recent advances on regenerative/neural engineering therapies for spinal cord injury,aiming at providing an insight to the most promising repair strategies,in order to facilitate future research conduction. | Magdalini Tsintou Kyriakos Dalamagkas Alexander Marcus Seifalian | 2015 | Neural Regeneration Research2015，10，5： | 8 |
| 3 | Role of nanotopography in the development of tissue engineered 3D organs and tissues using mesenchymal stem cells显示文摘Recent regenerative medicine and tissue engineering strategies(using cells, scaffolds, medical devices and gene therapy) have led to fascinating progress of translation of basic research towards clinical applications. In the past decade, great deal of research has focused on developing various three dimensional(3D) organs, such as bone, skin, liver, kidney and ear,using such strategies in order to replace or regenerate damaged organs for the purpose of maintaining or restoring organs' functions that may have been lost due to aging, accident or disease. The surface properties of a material or a device are key aspects in determining the success of the implant in biomedicine, as the majority of biological reactions in human body occur on surfaces or interfaces. Furthermore, it has been established in the literature that cell adhesion and proliferation are, to a great extent, influenced by the micro- and nanosurface characteristics of biomaterials and devices. In addition, it has been shown that the functions of stem cells, mesenchymal stem cells in particular, could be regulated through physical interaction with specific nanotopographical cues. Therefore, guided stem cell proliferation, differentiation and function are of great importance in the regeneration of 3D tissues and organs using tissue engineering strategies. This review will provide an update on the impact of nanotopography on mesenchymal stem cells for the purpose of developing laboratory-based 3D organs and tissues, as well as the most recent research and case studies on this topic. | Shima Salmasi Deepak M Kalaskar Wai-Weng Yoon Gordon W Blunn Alexander M Seifalian | 2015 | World Journal of Stem Cells2015，7，2： | 4 |
| 4 | Control of stem cell fate by engineering their micro and nanoenvironment显示文摘Stem cells are capable of long-term self-renewal and differentiation into specialised cell types, making them an ideal candidate for a cell source for regenerative medicine. The control of stem cell fate has become a major area of interest in the field of regenerative medicine and therapeutic intervention. Conventional methods of chemically inducing stem cells into specific lineages is being challenged by the advances in biomaterial technology, with evidence highlighting that material properties are capable of driving stem cell fate. Materials are being designed to mimic the clues stem cells receive in their in vivo stem cell niche including topographical and chemical instructions. Nanotopographical clues that mimic the extracellular matrix(ECM) in vivo have shown to regulate stem cell differentiation. The delivery of ECM components on biomaterials in the form of short peptides sequences has also proved successful in directing stem cell lineage. Growth factors responsible for controlling stem cell fate in vivo have also been delivered via biomaterials to provide clues to determine stem cell differentiation. An alternative approach to guide stem cells fate is to provide genetic clues including delivering DNA plasmids and small interfering RNAs via scaffolds. This review, aims to provide an overview of the topographical, chemical and molecular clues that biomaterials can provide to guide stem cell fate. The promising features and challenges of such approaches will be highlighted, to provide directions for future advancements in this exciting area of stem cell translation for regenerative medicine. | Michelle F Griffin Peter E Butler Alexander M Seifalian Deepak M Kalaskar | 2015 | World Journal of Stem Cells2015，7，1： | 3 |
| 5 | Haemoxygenase modulates cytokine induced neutrophil chemoattractant in hepatic ischemia reperfusion injury显示文摘AIM To investigate the hepatic microcirculatory changes due to Haemoxygenase(HO),effect of HO inhibition on remote ischemic preconditioning(RIPC) and modulation of CINC.METHODS Eight groups of animals were studied- Sham,ischemia reperfusion injury(IRI) the animals were subjected to 45 min of hepatic ischemia followed by three hours of reperfusion,RIPC(remote ischemic preconditioning) + IRI group,remote ischemic preconditioning in sham(RIPC + Sham),PDTC + IR(Pyridodithiocarbamate,HO donor),Zn PP + RIPC + IRI(Zinc protoporphyrin prior to preconditioning),IR-24(45 min of ischemia followed by 24 h of reperfusion),RIPC+IR-24(preconditioning prior to. After 3 and 24 h of reperfusion the animals were killed by exsanguination and samples were taken. RESULTS Velocity of flow(160.83 ± 12.24 μm/s),sinusoidal flow(8.42 ± 1.19) and sinusoidal perfusion index(42.12 ± 7.28) in hepatic IR were lower(P < 0.05) in comparison to RIPC and PDTC(HO inducer). RIPC increased velocity of flow(328.04 ± 19.13 μm/s),sinusoidal flow(17.75 ± 2.59) and the sinusoidal perfusion index(67.28 ± 1.82)(P < 0.05). PDTC(HO induction) reproduced the effects of RIPC in hepatic IR. PDTC restored RBC velocity(300.88 ± 22.109 μm/s),sinusoidal flow(17.66 ± 3.71) and sinusoidal perfusion(82.33 ± 3.5) to near sham levels. Zn PP(HO inhibition) reduced velocity of flow of RBC in the RIPC group(170.74 ± 13.43 μm/s and sinusoidal flow in the RIPC group(9.46 ± 1.34). Zn PP in RIPC(60.29 ± 1.82) showed a fall in perfusion only at 180 min of reperfusion. Neutrophil adhesion in IR injury is seen in both postsinusoidal venules(769.05 ± 87.48) and sinusoids(97.4 ± 7.49). Neutrophil adhesion in RIPC + IR injury is reduced in both postsinusoidal venules(219.66 ± 93.79) and sinusoids(25.69 ± 9.08)(P < 0.05). PDTC reduced neutrophil adhesion in both postsinusoidal venules(89.58 ± 58.32) and sinusoids(17.98 ± 11.01)(P < 0.05) reproducing the effects of RIPC. Zn PP(HO inhibition) increased venular(589.04 ± 144.36) and sinusoidal neutrophil adhesion in preconditioned animals(121.39 ± 30.65)(P < 0.05). IR after 24 h of reperfusion increased venular and sinusoidal neutrophil adhesion in comparison to the early phase and was significantly reduced by RIPC. Hepatocellular cell death in IRI(80.83 ± 13.03),RIPC + IR(17.35 ± 2.47),and PTDC+IR(11.66 ± 1.17) Zn PP + RIPC + IR(41.33 ± 3.07) reduced hepatocellular death. Zn PP significantly increased hepatocellular death(P < 0.05 PTDC/RIPC vs Zn PP and IR). The CINC cytokine levels in sham(101.32 ± 6.42). RIPC + sham(412.18 ± 65.24) as compared to sham(P < 0.05). Hepatic IR(644.08 ± 181.24)(P < 0.05). RIPC CINC-1 levels in the early phase(401.62 ± 78.56). And PDTC(HO inducer) CINC-1 levels in hepatic IR(413.36 ± 63.06) were significantly lower. HO inhibition in preconditioned animals with Zinc protoporphyrin increased serum CINC levels(521.81 ± 74.9)(P < 0.05). The serum CINC levels were high in the late phase of hepatic IR(15306 ± 1222.04). RIPC reduced CINC levels in the late phase of IR(467.46 ± 26.06),P < 0.05.CONCLUSION RIPC protects hepatic microcirculation by induction of HO and modulation of CINC in hepatic IR. | Niteen Tapuria Sameer Junnarkar Mahmoud Abu-amara Barry Fuller Alexander M Seifalian Brian R Davidson | 2016 | World Journal of Gastroenterology2016，22，33： | 3 |
| 6 | Nanosilver as a new generation of nanoproduct in biomedical applications显示文摘 | Karla Chaloupka Yogeshkumar Malam Alexander M. Seifalian | 2010 | Trends in Biotechnology2010，，11： | 2 |
| 7 | Protection of the liver by ischemic preconditioning: a review of mechanisms and clinical applications显示文摘 | Koti R S Seifalian A M Davidson B R | 2003 | Dig Surg2003，20，5： | 2 |
| 8 | Apoptosis and colorectal cancer: implications for therapy显示文摘 | Shi Yu Yang Kevin M. Sales Barry Fuller Alexander M. Seifalian Marc C. Winslet | 2009 | Trends in Molecular Medicine2009，，5： | 2 |
| 9 | The application of exosomes as a nanoscale cancer vaccine 显示文摘 | Tan A H De La Pena Seifalian A M | 2010 | Int J Nanomedicine2010，5，： | 1 |
| 10 | Induction of adhesion molecule expression in liver ischemia-reperfusion injury is associated with impaired hepatic parenchymal microcirculation 显示文摘 | Eiwahsh M Seifalian AM Fuller BJ | 2004 | Br J Surg2004，91，8： | 1 |
| 11 | Effect of graded hypoxia on the rat hepatic tissue oxygenation and energy metabolism monitored by near-infrared and 31P nuclear magnetic resonance spectroscopy显示文摘 | El-Desoky H Delpy D T | 2001 | FASEB J2001，15，14： | 1 |
| 12 | Graduated compression stockings in the prevention of venous thrombo- embolism显示文摘 | AGU O SEIFALIAN A HAMILTON G BAKER D | 1999 | Br J Surg1999，86，： | 1 |
| 13 | Nanosilver as a new generation of nanoproduct in biomedical applications显示文摘 | CHALOUPKA K MALA Y SEIFALIAN A M | 2010 | Trends Biotechnol2010，28，： | 1 |
| 14 | In vivo demonstration of impaired microcirculation in steatotic human liver grafts显示文摘 | Seifalian AM Chidambaram V Rolles K | 1998 | Liver Transpl Surg1998，4，1： | 1 |
| 15 | The role of established and emerging risk factors in peripheral vascular graft occlusion显示文摘 | Fatourou EM Paraskevas KI Seifalian AM | 2007 | Expert Opin Pharmacother2007，8，7： | 1 |
| 16 | The effect of mechanically enhancing portal venous inflow on hepatic oxygenation, microcirculation, and function in a rabbit model with extensive hepatic fibrosis 显示文摘 | Jiao LR Seifalian AM Habib N Davidson BR | 1999 | Hepatology1999，30，1： | 1 |
| 17 | Biochemical engineering nerve conduits using peptide amphiphiles显示文摘 | Tan A Rajadas J Seifalian AM | | 0，，03： | 1 |
| 18 | REVIEW: Ischemia—Reperfusion Injury of the Intestine and Protective Strategies Against Injury显示文摘 | Ismail Hameed Mallick Wenxuan Yang Marc C. Winslet Alexander M. Seifalian | 2004 | Digestive Diseases and Sciences2004，，9： | 1 |
| 19 | Effect of liver blood flow and function on hepatic indocyanine green clearance mea- sured directly in a cirrhotic animal model 显示文摘 | Jiao LR El-Desoky AA seifalian AM | 2000 | Br J Surg2000，87，5： | 1 |
| 20 | 显示文摘 | Ghanbari Hossein Cousins Brian G Seifalian Alexander M | 2011 | Macromol Rapid Commun2011，32，14： | 1 |
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