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| 1 | Seasonal variation of Microcystis in Lake Taihu and its relationships with environmental factors显示文摘In order to monitor the changes of Microcystis along with temporal and spatial variations, seasonal variation of Microcystis in Lake Taihu was investigated by 16S-23S rRNA internal transcribed spacer denaturing gradient gel electrophoresis (16S-23S rRNA-ITS DGGE) and microscopic evaluation. Samples were collected quarterly at four sites (River Mouth, Meiliang Bay, Cross Area, and Lake Center) from August 2006 to April 2007. Results showed that Microcystis dominated total phytoplankton abundance at the four sites in all seasons except winter. The average annual abundance of Microcystis was relatively high at River Mouth and Meiliang Bay, reaching 81.22 × 106 and 61.32 × 106 cells/L, respectively. For temporal variations, Shannon-Wiener diversity index (H ) according to DGGE profile revealed the richness of Microcystis in summer (H = 1.375 ± 0.034) and winter (H = 1.650 ± 0.032) was lower than that in spring (H = 2.078 ± 0.031) and autumn (H = 2.365 ± 0.032) (P < 0.05). While for spatial variations, the richness of Microcystis at River Mouth (H = 2.015 ± 0.074) was higher than at other sites during four seasons (P < 0.01). Very few dierences of Microcystis diversity in the same season were observed among the other three sites (P > 0.05). Canonical correspondence analysis (CCA) was performed to elucidate the relationships between Microcystis operational taxonomic units (OTUs) composition and the environmental factors. Results of CCA revealed that temperature was strongly positively correlated with the first axis (r = 0.963), while TSS was negative correlated with the second axis (r = -0.716). Phylogenetic tree based on the sequencing results of target bands on DGGE gel indicated that samples collected in summer and winter constituted two separated clusters. | TAN Xiao KONG Fanxiang ZENG Qingfei CAO Huansheng QIAN Shanqin ZHANG Min | 2009 | Journal of Environmental Sciences2009，21，7： | 16 |
| 2 | Cloning of HBsAg-encoded genes in different vectors and their expression in eukaryotic cells显示文摘AIM: To compare the efficiency of different plasmids as DNA vectors by cloning three HBsAg-encoded genes into two eukaryotic expression vectors, pRc/CMV and pSG5UTPL/Flag, and to express HBsAg S, MS, and LS proteins in SP2/0 cells, and to establish monoclone SP2/0 cell strains that are capable of expressing S or S2S proteins stably.METHODS: Segments of S, preS2-S, preS1-preS2-S genes of Hepatitis B virus were amplified by routine PCR and preS1S fragment was amplified by Over-Lap Extension PCR. The amplified segments were cleaved with restricted endonuclease Hind Ⅲ/Not Ⅰ followed by ligation with pRc/CMV, or BamHI/EcoR Ⅰ followed by ligation with pSG5UTPL/Flag. After the plasmid vectors were cleaved with the correspond enzymes, the amplified segments were inserted into pRc/CMV or pSGSUTPL/Flag plasmid vectors with T4DNA ligase. KOZAK sequence was added before the initial ATG code of each fragment using specific primer. The inserted segments in the recombinant plasmids were sequenced after subcloning. BALB/c mice myeloma cells (SP2/0 cell line) were transfected with the recombinant plasmids. The expressions of the different recombinants were compared by Western-blot, using a monoclonal anti-HBs antibody as the primary antibody and peroxidase-labeled multi-linker as the secondary. Stable SP2/0-pRc/CMV-S or SP2/0- pRc/CMV-MS clones were established through clone screening with G418.RESULTS: Fragments with anticipated size were harvested after PCR. After recombination and screening, the sequences of the inserted segments in the recombinants were confirmed to be S, preS2S, preSl-preS2S and preSlS encoding genes,determined by sequencing. The results of Western-blot hybridization were positive for the anticipated proteins.Among them, pRc/CMV-S or pRc/CMV-MS demonstrated the highest expressing their respective antigen.CONCLUSION: Eight recombinant plasmids expressing S,M, L or preSlS proteins are obtained. For hepatitis surface antigen expression in eukaryotic cells, the vector pRc/CMV is superior to pSG5UTPL/Flag, and pRc/CMV-S and pRc/CMVMS are the most efficient in the pRc/CMV clones. SP2/0 cells stably expressing HBsAg are established, and may be used as target cells for evaluating the CTL activity of a DNA vaccine in vitro. | ShanQin HongTang Lian-SanZhao FangHe YongLin LiLiu Xiao-MeiHe | 2003 | World Journal of Gastroenterology2003，9，5： | 12 |
| 3 | Enrichment of heavy metal elements and their adsorption on iron oxides during carbonate rock weathering process显示文摘Analysis of trace elements in the Lower Palaeozoic carbonate rock strata of the Beijing area shows that the Cr, Zn, Cd and Pb contents increase from primary carbonate rocks to weathered carbonate rocks and from primary carbonate rocks to the soil coexisting with carbonate rock. Distributions of Cr, Zn, Cd and Pb have the same trends as that of Fe2O3. Iron oxides play different roles at different stages of weathering of carbonate rock. The adsorption of Cr, Zn, Cd and Pb on Fe2O3 is greater than on FeO in soil. However, FeO, as a main component of clay mineral, is a better adsorbent of Cr, Zn, Cd and Pb than Fe2O3 in weathered and primary carbonate rocks. According to R-type clustering analysis, the main components of carbonate rock are divided into marine and continental sediments. Correlation analysis shows that heavy metal elements mainly exist in continental substances. | Shanqin Ni Yiwen Ju Quanlin Hou Shijie Wang Qing Liu Yudong Wu Lingling Xiao | 2009 | Progress in Natural Science:Materials International2009，19，9： | 8 |
| 4 | Heavy metal content in scalp hair of the inhabitants near Dexing Copper Mine,Jiangxi Province,China显示文摘Levels of seven metals(Mn,Cu,Zn,Pb,Cr,Cd,and Se) in the scalp hair of Daicun and Xiangtian inhabitants were determined by ICP-MS/AFS.Daicun,as an experimental site,is near Dexing Copper Mine,Jiangxi Province,China,and exposed to mining pollution;Xiangtian,as a control site,is free from any mining and other industrial pollution.The heavy metal distribution in the scalp hair of the two diverse population segments exposed to different environments was discussed against the background of the heavy metal content of local plants,waters,and soils.The results show:1) Levels of Cu,Zn,Pb,Cr,and Cd in the scalp hair of Daicun inhabitants were higher compared with Xiangtian counterparts which showed higher comparative levels of Se and Mn.But there were no significant differences of the levels of Cu and Zn in the hair samples between the two villages.In the hair of the inhabitants in the two villages,the levels for Se,Zn,and Cu were lower,while the level for Mn was higher,than the corresponding levels from other regions of the world.Compared with the standard issued by the Trace Element Research Council of China for Chinese inhabitants there were serious Se,Zn,Cu and Cr-deficiency phenomena in the hair samples from Daicun and Xiangtian.2) Against the geological background of high levels of Zn,Cu,Se and Cr,there were deficiencies of Zn,Cu,Se and Cr in the hair samples of the Daicun population.This may have been caused by the distribution forms of the heavy metals in the soils,and antagonism among the elements.3) Principal Component Analysis(PCA) and Cluster Analysis(CA) indicated that the origins of the heavy metals in the hair samples from the two villages had some differences as well as some similarities,this should be contributed mainly to the influence of the mining activities. | NI ShanQin LI RuiPing WANG AnJian | 2011 | Science China Earth Sciences2011，54，5： | 6 |
| 5 | Effectiveness and safety of traditional Chinese herbs in children with cough variant asthma: a systematic review and Meta-analysis显示文摘OBJECTIVE:To systematically investigate the clinical effectiveness and safety of traditional Chinese herbs(TCHs)as an alternative to conventional medicine(CM)in children with cough variant asthma(CVA).METHODS:Randomized controlled trial(RCT)studies that were published from their inceptions to March 31,2020,were identified from the electronic databases of China National Knowledge Infrastructure,Wangfang,Pub Med,and Cochrane Central Library.The primary outcome of the review was the total effective rate(TER),and the secondary outcomes were immunoglobulin E(Ig E),peak expiratory flow(PEF),adverse drug reactions,and relapse rates of interventions.RESULTS:For the Meta-analysis,13 studies involving 992 children with CVA were included.In terms of TER and Ig E,the experimental interventions of TCH,when compared with the control interventions of CM,on pediatric CVA were found to be significantly effective(P<0.0001),whereas for spirometry,PEF was not significantly improved in the TCH group(P=0.48).The incident rates of adverse drug reaction and relapse were found to be significantly lower in the TCH group than those in the CM group(P=0.02 and P<0.0001,respectively).CONCLUSION:Compared with CM therapy,the effects of TCH therapy on pediatric CVA were significantly beneficial in terms of TER and Ig E,but not for PEF,and the methodological quality of included studies was poor.Therefore,the results should be interpreted with caution.More randomized controlled trials with rigorous experimental methodologies are required for objectivity in the future. | Kyou-Hwan Han Ki Haeng Cho CUI Shanqin Lily Lin Jaejong Kim | 2021 | Journal of Traditional Chinese Medicine2021，41，5： | 6 |
| 6 | Terrestrial Mio-Pliocene Boundary in the Linxia Basin,Gansu,China显示文摘在 Gansu 省的 Linxia 盆的更低的上新世是仅仅为在北中国,并且甚至在东亚的早上新世沉积记录的一些代表性的节之一。最近,丰富的哺乳动物的石块在这盆以内在 Guanghe 县在 Duikang 从更低的上新世 Hewangjia 形成的红泥土的底被发现。以前,上新世哺乳动物稀疏地在中国被发现,并且最从在东方黄土高原的河、湖的存款是镇定的。从广泛地分布式的上新世 Hipparion 红克莱的哺乳动物是在数字的更少。从 Duikang 的已知的石块包括 20 种类并且属于 Shilidun 动物志。他们的动物区系的部件类似于从 Yushe 的早上新世 Gaozhuang 动物志,山西。在另一方面,从 Duikang 的某 taxa 没在 Gaozhuang 动物志被发现,在进化水平是稍微更原始的,并且在主要出现晚中新世。作为结果, Duikang 石块的年龄可能比 Gaozhuang 动物志的稍微早、离上新世的更低的边界更靠近。Duikang 含化石的床是在晚中新世的 Liushu 形成,和三马 Hipparion pater 的第一出现的顶上面的 0.8 m 能被认为是 Miocene/Pliocene 边界的一个 biostratigraphical 标记。在结论, Duikang 是一个理想的候选人地区作为中国陆上的上新世的更低的边界的 stratotype 建立。 | DENG Tao HOU Sukuan SHI Qinqin CHEN Shaokun HE Wen CHEN Shanqin | 2011 | Acta Geologica Sinica(English Edition)2011，85，2： | 4 |
| 7 | Development of a reverse transcription quantitative polymerase chain reaction-based assay for broad coverage detection of African and Asian Zika virus lineages显示文摘The Zika virus(ZIKV) is an arbovirus that has spread rapidly worldwide within recent times. There is accumulating evidence that associates ZIKV infections with Guillain-Barré Syndrome(GBS) and microcephaly in humans. The ZIKV is genetically diverse and can be separated into Asian and African lineages. A rapid, sensitive, and specific assay is needed for the detection of ZIKV across various pandemic regions. So far, the available primers and probes do not cover the genetic diversity and geographic distribution of all ZIKV strains. To this end, we have developed a one-step quantitative reverse transcription polymerase chain reaction(qRT-PCR) assay based on conserved sequences in the ZIKV envelope(E) gene. The detection limit of the assay was determined to be five RNA transcript copies and 2.94 × 10^(–3) 50% tissue culture infectious doses(TCID50) of live ZIKV per reaction. The assay was highly specific and able to detect five different ZIKV strains covering the Asian and African lineages without nonspecific amplification, when tested against other flaviviruses. The assay was also successful in testing for ZIKV in clinical samples. Our assay represents an improvement over the current methods available for the detection ZIKV and would be valuable as a diagnostic tool in various pandemic regions. | Yang Yang Gary Wong Baoguo Ye Shihua Li Shanqin Li Haixia Zheng Qiang Wang Mifang Liang George F Gao Lei Liu Yingxia Liu Yuhai Bi | 2017 | Virologica Sinica2017，32，3： | 3 |
| 8 | Foraging behavior of Chleuastochoerus(Suidae, Artiodactyla): A case study of skull and mandible morpho-functional analysis显示文摘The skull and mandible of a Late Miocene fossil pig,Chleuastochoerus,are compared morphologically with those of extant pigs and peccaries,and subjected to a functional analysis.The presence of a rostral bone in the skull and relatively strong rostral muscles indicates that Chleuastochoerus possessed considerable digging ability,though the relatively narrow occipital surface and the shortness of the skull would have restricted the range of motion through which the skull could be swung.The distinctive pre-zygomatic plate and over-canine arch-niche may have played a role in protecting the skull and canines during digging.The massive masticatory muscles,reconstructed based on well-developed parietal and zygomatic crests,would have enabled the mouth to close quickly and contributed to moving the mandible both longitudinally and laterally.The configuration of the cranio-mandibular joint and its relatively high position show clearly that Chleuastochoerus was capable of both crushing and grinding movements during mastication.The comparatively simple structure of the crown surfaces of the cheek teeth suggests that the food of Chleuastochoerus may have been softer than that of extant suids.The diet of Chleuastochoerus is postulated to fall between those of true forest pigs and open habitat pigs,and the habitat of Chleuastochoerus to be relatively humid forest edge or nearby areas of open steppe. | HOU SuKuan DENG Tao HE Wen CHEN ShanQin | 2014 | Science China Earth Sciences2014，57，5： | 2 |
| 9 | AMPK Phosphorylates and Inhibits SREBP Activity to Attenuate Hepatic Steatosis and Atherosclerosis in Diet-Induced Insulin-Resistant Mice显示文摘 | Yu Li Shanqin Xu Maria M. Mihaylova Bin Zheng Xiuyun Hou Bingbing Jiang Ogyi Park Zhijun Luo Etienne Lefai John Y.-J. Shyy Bin Gao Michel Wierzbicki Tony J. Verbeuren Reuben J. Shaw Richard A. Cohen Mengwei Zang | 2011 | Cell Metabolism2011，，4： | 2 |
| 10 | Polyphenols stimulate AMP-activated protein kinase, lower lipids, and inhibit accelerated atherosclemsis in diabetic LDL receptor-deficient mice 显示文摘 | ZANG Mengwei XU Shanqin MAITLAND-TOOLAN K A | 2006 | Diabetes2006，55，8： | 1 |
| 11 | Occurrence Reasons and Control Measures of Caligula Japonica in Longnan City of China显示文摘Caligula Japonica has continuously broken out in large scale in Longnan City,Gansu Province of China,causing heavy economic losses. In order to effectively control the insect and to increase the production benefit of walnut in Longnan,the authors have investigated and observed the morphological characters and living habits of C. Japonica. The major causes of continuous large-scale occurrence of C. Japonica in Longnan City are analyzed concretely,and the integrated control measures are put forward from the aspects of silvicultural measures,physical control,chemical control and biological control. | Qiao Xu Wang Yicun Wu Gang Wang Siyuan Hu Jizhou Liu Tiexin Feng Shanqin | 2014 | Plant Diseases and Pests2014，5，5： | 1 |
| 12 | A new flavonol glycoside from glandless cotton seeds显示文摘A new flavonol glycoside,namely quercetin 3-O-[a-D-apiofuranosyl(1–5)-b-D-apiofuranosyl(1–2)]-a-L-rhamnopyranosyl(1–6)-b-D-glucopyranoside(1),was isolated from glandless cotton seeds together with the known compounds quercetin 3-O-a-L-rhamnopyranosyl(1–2)-[a-Lrhamnopyranosyl(1–6)]-b-D-glucopyranoside(manghaslin,2),kaempferol 3-O-b-D-apiofruranosyl(1–2)-b-D-glucopyranoside(3)and kaempferol 3-O-a-L-rhamnopyranosyl(1–6)-b-D-glucopyranoside(4).It is interesting that the tetrasaccharide fragment of 1 contained both a b-apiosyl and an unusual a-apiosyl group. | Shanqin Yuan Ming Yang Yimin Zhao | 2012 | Acta Pharmaceutica Sinica B2012，2，1： | 1 |
| 13 | AMPK phosphorylates and inhibits SREBP activity to attenuate hepatic steatosis and atherosclerosis in diet-induced insulin- resistant mice显示文摘 | LI Yu XU Shanqin MIHAYLOVA M M | 2011 | Cell Metab2011，13，4： | 1 |
| 14 | SIRT1Regu-lates Hepatocyte Lipid Metabolism through ActivatingAMP-activated Protein Kinase显示文摘 | Xiuyun Hou Shanqin Xu Sato K | 2008 | Journal of BiolChem2008，283，20： | 1 |
| 15 | Allyl mercaptan,a major metabolite of garlic compounds,reduces cellular cholesterol synthesis and its secretion in Hep-G2 cells显示文摘 | Shanqin Xu BH Simon Cho | 1999 | J Nutr Biochem1999，10，： | 1 |
| 16 | Allyl mercaptan, a major metabolite of garlic compounds, reduces cellular cholesterol synthesis and its secretion in Hep-G cells显示文摘 | XU Shanqin CHO S B H | 1999 | J Nutr Biochem1999，10，： | 1 |
| 17 | Activation of Thromboxane Receptor Upregulates Interleukin (IL)-1β-Induced VCAM-1 Expression Through JNK Signaling显示文摘 | Hossein Bayat Shanqin Xu David Pimentel | 2008 | Arterioscler Thromb Vasc Biol2008，28，1： | 1 |
| 18 | Migration and enrichment of trace elements of Lower Palaeozoic carbonate rock strata in Beijing显示文摘Analyses of trace elements of the Lower Palaeozoic carbonate rock strata in Beijing show that the contents of As, Hg, F increase from primary carbonate rocks to weathered carbonate rocks and from primary carbonate rocks to the soil coexisting with carbonate rocks, but the distribution regularity of S is not obvious. In the whole weathered stages, the sorption of As is mainly affected by Fe2O3. In soil Fe2O3 is also the main affecting factor of Hg enrichment. The main existing forms of Hg in primary carbonate rocks should simply be physical adsorption, coprecipitation and false isomorphous form between surface of carbonate rock and Hg. In soil the enrichment of F has little relationship with sul-fides and Fe2O3. In primary carbonate rocks, F is mainly absorbed by sulfides and clay minerals, etc. Weathered samples have closer genetic relationships with primary carbonate rocks. This also implies that weathered carbonate rocks have the close existing forms to that of primary carbonate rocks. In primary carbonate rocks FeS2 and FeS are the main forms of S, and sulfides have fixation effect on some heavy metals, whereas in weathered carbonate rocks and soil the fixation effect is weakened. | NI ShanQin HOU QuanLin JU YiWen XIAO LingLing WU YuDong LIU Qing | 2008 | Science China Earth Sciences2008，51，12： | 1 |
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