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| 1 | Direct and single-step sensing of primary ovarian cancers related glycosidases显示文摘High sensitive,accurate detection for tumor-associated overexpressed enzyme activity is highly significant for further understanding enzyme function,discovering potential drugs,and early diagnosis and prevention of diseases.In this work,we developed a facile,direct and single-step detection platform for primary ovarian cancers related glycosidase activity based on the inner filter effect(IFE)between glycosidase catalytic product and black phosphorus quantum dots(BPQDs).Highly fluorescent BPQDs were successfully synthesized from bulk black phosphorus by a simple liquid exfoliation method.Under the catalysis ofβ-galactosidase,p-nitrophenyl-β-D-galactopyranoside(PNPG)was transformed into pnitrophenol(PNP)andβ-D-galactopyranoside.Meanwhile,the absorption of catalytic product PNP greatly overlapped with the excitation and emission spectra of fluorescent BPQDs,leading to the fluorescence quenching of BPQDs with a high quenching efficiency.The proposed sensing strategy provided a low detection limit of 0.76 U/L,which was 1-2 orders of magnitude lower than most unmodified sensing platforms.D-Galactal was selected as the inhibitor forβ-galactosidase to further assess the feasibility of screening potential inhibitors.The fluorescence recovery of BPQDs suggests that the unmodified sensing platform is feasible to discover potential drugs ofβ-galactosidase.Our work paves a general way in the detection of glycosidase activity with fluorescent BPQDs,which can be promising for glycosidase-related disease diagnosis and pathophysiology elucidation. | Dan Li Ling Liang Yawei Tang Linna Fu Shehua Xiao Quan Yuan | 2019 | Chinese Chemical Letters2019，30，5： | 2 |
| 2 | A quantitative assessment of follicle size on oocyte developmen- tal competence 显示文摘 | Mitchell P Rosen MD Shehua Shen MD Anthony T Dobson MD PhD | 2008 | Fertility and Slerility2008，90，3： | 1 |
| 3 | Glabridin from Chinese herb licorice inhibits fatigue in mice 显示文摘 | SHANG Huaping CAO Shehua WANG Jihui | 2010 | African Journal of Traditional Complementary and Ahemative Medicines: AJTCAM/African Networks on Ethnomedicines2010，7，1： | 1 |
| 4 | Biomarkers identified with time-lapse imaging – Discovery, validation, and practical application显示文摘 | Alice A. Chen Lei Tan Vaishali Suraj Renee Reijo Pera Shehua Shen | 2013 | Fertility and Sterility2013，，： | 1 |
| 5 | A numerical study of turbulent flow and conjugate heat transfer in concentric annuli with moving inner rod显示文摘 | HUANG Shehua CHUN Chunghwan | 2003 | International Journal of Heat and Mass Transfer2003，46，： | 1 |
| 6 | Improving embryo selection using a computer-automated time-lapse image analysis test plus day 3 morphology: results from a prospective multicenter trial显示文摘 | Joe Conaghan Alice A. Chen Susan P. Willman Kristen Ivani Philip E. Chenette Robert Boostanfar Valerie L. Baker G. David Adamson Mary E. Abusief Marina Gvakharia Kevin E. Loewke Shehua Shen | 2013 | Fertility and Sterility2013，，2： | 1 |
| 7 | The role of endoplasmic reticulum stress in endothelial dysfunction induced by homocysteine thiolactone显示文摘 | Shujin Wu Xiang Gao Shehua Yang Min Meng Xiaolai Yang Bin Ge | 2015 | Fundam Clin Pharmacol2015，，： | 1 |
| 8 | Two variation of finite automata public key cryptosystem and digital signatures显示文摘 | Tao RenJi Chen Shehua | 1986 | Computer Science and Technology1986，1，1： | 1 |
| 9 | Photoresponsive Biomimetic Protocells for Near-Infrared-Light- Regulated Phototheranostics显示文摘Nature has created complex living systems with outstanding structure and remarkable function.Constructing biomimetic systems that rival living organisms has attracted considerable research inter-est in research fields of self-assembly and bionic sci-ence. | Ling Liang Yawei Tang Xiaoxiao Hu Jie Wang Shehua Xiao Dan Li Linna Fu Zhihao Li Quan Yuan | 2019 | CCS Chemistry2019，1，4： | 0 |
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