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| 1 | Comparative proteogenomic analysis of the Leptospira interrogans virulence-attenuated strain IPAV against the pathogenic strain 56601显示文摘稀释毒力的螺旋体 interrogans serovar Lai 紧张 IPAV 被延长实验室经过从在中国孤立的高度剧毒的祖先的紧张导出。我们学习了 IPAV 的基因变化对病原的 L 经由比较分析使它变为无毒害。interrogans serovar Lai 紧张 56601。IPAV 紧张的完全的染色体顺序决心、使用与作比较,然后修正并且重新注解紧张 56601 的染色体顺序。除了他们的高度类似的 genomic,结构和基因顺序, 33 插入的一个总数, 53 删除和 301 个单个核苷酸的变化(SNV ) 在整个直接影响 101 基因的 IPAV 的染色体被检测,在他们的 5 ′ 的任何一个;在上游的区域或在他们的编码区域以内。在他们之中, 44 功能的基因的多数涉及信号 transduction,压力反应, transmembrane 运输和氮新陈代谢。基于量的液体,层析(LC )-MS/MS 数据揭示了的比较 proteomic 分析在 orthologs 的 1 627 选择的对之中,在 IPAV 的 174 基因拉紧,这是 upregulated,与主要在精力的班上的丰富生产和类脂化合物新陈代谢。相反,在紧张 56601 的 228 基因是 upregulated,与在蛋白质翻译和 DNA 复制 / 修理的范畴充实的多数。 genomic 和 proteomic 途径的联合在批评基因说明了那改变的表情或变化,例如编码 Ser/Thr kinase ,碳饥饿蛋白质 CstA ,夫酸安合成酶,GTP有约束力的蛋白质 BipA , ribonucleotide-diphosphate reductase 和磷酸盐 transporter 的那些,并且在脂蛋白或外部膜蛋白质的翻译侧面的改变是可能的在紧张 IPAV 说明毒力变细。 | Yi Zhong Xiao Chang Xing-Jun Cao Yan Zhang Huajun Zheng Yongzhang Zhu Chengsong Cai Zelin Cui Yunyi Zhang Yuan-Yuan Li Xiu-Gao Jiang Guo-Ping Zhao Shengyue Wang Yixue Li Rong Zeng Xuan Li Xiao-Kui Guo | 2011 | Cell Research2011，21，8： | 10 |
| 2 | Effect of the W-beam central guardrails on wind-blown sand deposition on desert expressways in sandy regions显示文摘Many desert expressways are affected by the deposition of the wind-blown sand,which might block the movement of vehicles or cause accidents.W-beam central guardrails,which are used to improve the safety of desert expressways,are thought to influence the deposition of the wind-blown sand,but this has yet not to be studied adequately.To address this issue,we conducted a wind tunnel test to simulate and explore how the W-beam central guardrails affect the airflow,the wind-blown sand flux and the deposition of the wind-blown sand on desert expressways in sandy regions.The subgrade model is 3.5 cm high and 80.0 cm wide,with a bank slope ratio of 1:3.The W-beam central guardrails model is 3.7 cm high,which included a 1.4-cm-high W-beam and a 2.3-cm-high stand column.The wind velocity was measured by using pitot-static tubes placed at nine different heights(1,2,3,5,7,10,15,30 and 50 cm)above the floor of the chamber.The vertical distribution of the wind-blown sand flux in the wind tunnel was measured by using the sand sampler,which was sectioned into 20 intervals.In addition,we measured the wind-blown sand flux in the field at K50 of the Bachu-Shache desert expressway in the Taklimakan Desert on 11 May 2016,by using a customized 78-cm-high gradient sand sampler for the sand flux structure test.Obstruction by the subgrade leads to the formation of two weak wind zones located at the foot of the windward slope and at the leeward slope of the subgrade,and the wind velocity on the leeward side weakens significantly.The W-beam central guardrails decrease the leeward wind velocity,whereas the velocity increases through the bottom gaps and over the top of the W-beam central guardrails.The vertical distribution of the wind-blown sand flux measured by wind tunnel follows neither a power-law nor an exponential function when affected by either the subgrade or the W-beam central guardrails.At 0.0H and 0.5H(where H=3.5 cm,which is the height of the subgrade),the sand transport is less at the 3 cm height from the subgrade surface than at the 1 and 5 cm heights as a result of obstruction by the W-beam central guardrails,and the maximum sand transportation occurs at the 5 cm height affected by the subgrade surface.The average saltation height in the presence of the W-beam central guardrails is greater than the subgrade height.The field test shows that the sand deposits on the overtaking lane leeward of the W-beam central guardrails and that the thickness of the deposited sand is determined by the difference in the sand mass transported between the inlet and outlet points,which is consistent with the position of the minimum wind velocity in the wind tunnel test.The results of this study could help us to understand the hazards of the wind-blown sand onto subgrade with the W-beam central guardrails. | WANG Cui LI Shengyu LEI Jiaqiang LI Zhinong CHEN Jie | 2020 | Journal of Arid Land2020，12，1： | 2 |
| 3 | Tri bologieal properties of bronze-graphite composites under sea water condition显示文摘 | Cui Gongjun Bi Qinling Zhu Shengyu | 2012 | Tribology Interna- tional2012，53，： | 1 |
| 4 | Tribological prop-erties of bronze-graphite composites under sea water condition显示文摘 | Cui Gongjun Bi Qinling Zhu Shengyu | 2012 | Tribology International2012，53，： | 1 |
| 5 | 3D FEM Simulation of Milling Force in Corner Machining Process显示文摘To optimize milling force and machining accuracy quality in corner milling process, the changing law of milling force is revealed by Finite Element Method(FEM).Based on DEFORM software a serial of 3D FEM models for corner milling process are devloped. Tool curved trajectory is achieved by establishing accurate relationship of tool location with milling time. Adaptive remeshing technique and iterative algorithm are adopted to ensure convergence of FEM model. Component force characteristics are revealed by analyzing FEM simulation results. It indicates that the milling force in Y direction becomes negative comparing with forces in X and Z direction.Magnitude of forces in three directions decreases with increase of spindle speed, while it increases with increase of milling feedrate. The simulation results for cutting force are in good agreement with those obtained from experiment. The FEM simulation model is first successfully established for corner milling process in this study, and the results provide a guide for optimizing cutting parameters in cutting process. | Caixu YUE Cui HUANG Xianli LIU Shengyu HAO Jun LIU | 2017 | Chinese Journal of Mechanical Engineering2017，30，2： | 1 |
| 6 | Study of polysiloxanes containing epoxy groups: I Synthesis and characterization of polysiloxanes containing 3-(2,3-epoxypropoxy)propyl groups显示文摘 | FENG SHENGYU CUI MENGZHONG | 2000 | Reactive & Functional Polymers2000，45，2： | 1 |
| 7 | Tribological properties of bronze–graphite composites under sea water condition显示文摘 | Gongjun Cui Qinling Bi Shengyu Zhu Jun Yang Weimin Liu | 2012 | Tribology International2012，，： | 1 |
| 8 | Tribological properties of bronze–graphite composites under sea water condition显示文摘 | Gongjun Cui Qinling Bi Shengyu Zhu Jun Yang Weimin Liu | 2012 | Tribology International2012，，： | 1 |
| 9 | Fibroblast growth factor 5 overexpression ameliorated lipopolysaccharide-induced apoptosis of hepatocytes through regulation of the phosphoinositide-3-kinase/protein kinase B pathway显示文摘Background:Sepsis is a systemic inflammatory syndrome induced by several infectious agents.Multiple organs are affected by sepsis,including the liver,which plays an important role in metabolism and immune homeostasis.Fibroblast growth factors(FGFs)participate in several biological processes,although the role of FGF5 in sepsis is unclear.Methods:In this study,lipopolysaccharide(LPS)was administrated to mice to establish a sepsis-induced liver injury.A similar in vitro study was conducted using L-02 hepatocytes.Western blot and immunohistochemistry staining were performed to evaluate the FGF5 expression level in liver tissues and cells.Inflammatory cell infiltrations,cleaved-caspase-3 expressions,reactive oxygen species and levels of inflammatory cytokines were detected by immunofluorescence,dihydroethidium staining,and reverse transcription quantitative polymerase chain reaction analysis,respectively.Flow cytometry was used to detect the apoptosis level of cells.In addition,ribonucleic acid(RNA)-sequencing was applied to explore the possible mechanism by which FGF5 exerted effects.Results:LPS administration caused FGF5 down-regulation in the mouse liver as well as in L-02 hepatocytes.Additionally,with FGF5 overexpression,liver injury and the level of hepatocyte apoptosis were ameliorated.Further,RNA sequencing performed in hepatocytes revealed the phosphoinositide-3-kinase/protein kinase B(PI3K/AKT)pathway as a possible pathway regulated by FGF5.This was supported using an inhibitor of the PI3K/AKT pathway,which abrogated the protective effect of FGF5 in LPSinduced hepatocyte injury.Conclusion:The anti-apoptotic effect of FGF5 on hepatocytes suffering from LPS has been demonstrated and was dependent on the activation of the PI3K/AKT signaling pathway. | Shengyu Cui Yuhua Li Xutao Zhang Bing Wu Ming Li Jixian Gao Lin Xu Hao Xia | 2022 | Chinese Medical Journal2022，135，23： | 0 |
| 10 | Unconventional metamagnetic phase transition in R_(2)In(R=Nd, Pr)with lambda-like specific heat and nonhysteresis显示文摘The phase transition of Nd_(2)In and Pr_(2)In has been studied and found to be between the first-order and second-order nature.Hardly any hysteresis nor obvious cell volume changes are presented.Concurrently the field-triggered metamagnetism is demonstrated with lambda-like peak on specific heat curve.For the field change of 7 T,giant magnetic entropy changes of 13.2 J kg^(-1)K^(-1) and 19.5 J kg^(-1)K^(-1) and adiabatic temperature changes of 6.5 K and 7.4 K have been achieved in Nd_(2)In and Pr_(2) In alloys respectively,which is partially contributed by strong magnetoelastic coupling represented by high saturation magnetostriction of~450 ppm.Such unique feature is proposed to be originated from the delocalized 5d electrons,as evidenced by the negative magnetoresistance caused by the phase transition. | Weibin Cui Guiquan Yao Shengyu Sun Qiang Wang Jie Zhu Sen Yang | 2022 | Journal of Materials Science & Technology2022，，6： | 0 |
| 11 | Green biomanufacturing promoted by automatic retrobiosynthesis planning and computational enzyme design显示文摘Biomanufacturing,which uses renewable resources as raw materials and uses biological processes to produce energy and chemicals,has long been regarded as a production model that replaces the unsustainable fossil economy.The construction of non-natural and efficient biosynthesis routes of chemicals is an important goal of green biomanufacturing.Traditional methods that rely on experience are difficult to support the realization of this goal.However,with the rapid development of information technology,the intelligence of biomanufacturing has brought hope to achieve this goal.Retrobiosynthesis and computational enzyme design,as two of the main technologies in intelligent biomanufacturing,have developed rapidly in recent years and have made great achievements and some representative works have demonstrated the great value that the integration of the two fields may bring.To achieve the final integration of the two fields,it is necessary to examine the information,methods and tools from a bird’s-eye view,and to find a feasible idea and solution for establishing a connection point.For this purpose,this article briefly reviewed the main ideas,methods and tools of the two fields,and put forward views on how to achieve the integration of the two fields. | Ziheng Cui Shiding Zhang Shengyu Zhang Biqiang Chen Yushan Zhu Tianwei Tan | 2022 | Chinese Journal of Chemical Engineering2022，35，1： | 0 |
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