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| 1 | One-step generation of myostatin gene knockout sheep via the CRISPR/Cas9 system显示文摘The CRISPR/Cas(clustered regularly interspaced short palindromic repeats/CRISPR-associated)adaptive immune system,which was discovered in bacteria and archaea,can specifically degrade invasive viral and plasmid DNA by base pairing between crRNAs(CRISPR RNAs)and the target DNA[1,2].Recently,the Streptococcus pyogenes type II CRISPR system was shown to be able to perform efficient targeted gene disruption by employing three fundamental components:(1)Cas9 endonuclease. | Hongbing HAN Yonghe MA Tao WANG Ling LIAN Xiuzhi TIAN Rui HU Shoulong DENG Kongpan LI Feng WANG Ning LI Guoshi LIU Yaofeng ZHAO Zhengxing LIAN | 2014 | Frontiers of Agricultural Science and Engineering2014，1，1： | 8 |
| 2 | The Zα domain of fish PKZ facilitates the B-Z conformational transition of oligonucleotide DNAs with d(GC), inserts显示文摘PKZ (象 PKR 一样) 在鱼作为 elF2 kinase 家庭的一个成员被发现,它在 C 终端并且也拥有 eIF2 kinase 的一个保存催化领域在 N 终端的二个 Z-DNA-binding 领域(Z1 和 Z2 ) 。PKZ 能通过 Z 的绑定被激活到 Z-DNA。然而, PKZ Z 的规章的功能仍然保持不清楚。调查 PKZ Z 怎么与 Z-DNA 交往的分子的机制,我们在 Escherichia coli 罗塞达碑紧张表示了 Z 多肽 Z12 并且在 Ni-NTA 上由亲密关系层析净化了树脂。oligonucleotide DNA 与的不同长度各种各样插入,也就是 d (GC ) n (n = 6, 8, 10, 13 ) , d (TA ) n (n = 6, 10 ) , non-d (GC ) ,和 non-d (TA ) ,被设计并且综合。圆形的二色性光谱和胶化活动性移动试金被用来在不同 oligonucleotide DNA 的 conformational 转变上调查 Z12 的效果。结果证明 oligonucleotide DNA 当 Z12 不在时保留了常规 B-DNA 符合构造。与 Z12 滴定的增加的数量, d (GC ) n 被认出并且在某种程度上把符合构造变换成 Z-DNA。与增加重复数字(从 n = 6 到 n = 13 ) , conformational 转变的趋势变得更明显。然而,有 n 插入的 d (TA ) 的 oligonucleotides 的符合构造面对 Z12 有点变化了,并且 Z12 没在有 non-d (GC ) 或 non-d (TA ) 的 oligonucleotides 的转变插入的 conformational 上有效果。胶化活动性移动试金进一步证明 Z12 能与 d (GC ) 绑在 oligonucleotide 10。换句话说, Z12 能与 n 插入到 Z-DNA 符合构造的 d (GC ) 转 oligonucleotides 并且与高亲密关系绑在它。 | Puzhong Lu Shoulong Deng Youlin Zhu Yongbin Yan Yong Liu Chengyu Hu | 2012 | Acta Biochimica et Biophysica Sinica2012，44，11： | 3 |
| 3 | The Zα domain of fish PKZ converts DNA hairpin with d(GC)n inserts to Z-conformation显示文摘PKZ,蛋白质 kinase 包含 Z-DNA 领域,是脊椎动物 eIF2 kinase 家庭的一个新奇成员。在 N 终点在 C 终点和二个 Z-DNA 有约束力的领域(Z1 和 Z2 ) 包含一个催化领域, PKZ 能通过 Z 的绑定被激活到 Z-DNA。然而, PKZ Z 的规章的功能尚待被证实。这里,蛋白质被表示在 DNA conformational 转变,野类型的 Z1Z2 和 11 异种上理解 PKZ Z 的影响并且净化。同时, DNA hairpinsd (GC ) 的几不同长度 nT4 (GC ) n (n = 26 ) 并且 RNA hairpinr (GC ) 6T4 (GC ) 6 被综合。这些合成 DNA 或 RNA 发卡的符合构造上的 Z1Z2 和变异的蛋白质的效果被使用圆形的二色性光谱和胶化活动性移动试金调查。结果证明 DNA 发卡当 Z1Z2 不在时保留了常规 B-DNA 符合构造,当一些 DNA 发卡(n3 ) 在 Z1Z2 正式就职下面被变换成 Z 符合构造时。趋势按比例与 GC 重复的增加的数量被联系。与 Z1Z2 比较, Z1Z1 而非 Z2Z2 在变换 d (GC ) 显示了一个更高的能力 6T4 (GC ) 6 从 B- 到 Z-DNA。这些结果证明那 Z1 子域比 Z2 子域在 BZ conformational 转变的过程起了一个更必要的作用。变异的蛋白质(K34A, N38A, R39A, Y42A, P57A, P58A,和 W60A ) 不能变换 d (GC ) 6T4 (GC ) 6 进 Z-DNA,而 S35A 或 K56A 保留了一些部分活动。有趣地, Z1Z2 也能导致 r (GC ) 6T4 (GC ) 从 A 符合构造的 6 RNA 到在适当条件下面的 Z 符合构造。 | Puzhong Lu Shoulong Deng Chuxin Wu Youlin Zhu Yong Liu Gang Lin Yongbin Yan Chengyu Hu | 2013 | Acta Biochimica et Biophysica Sinica2013，45，12： | 2 |
| 4 | Over-expression of Toll-like receptor 2 up-regulates heme oxygenase-1 expression and decreases oxidative injury in dairy goats显示文摘Background: Mastitis, an infection caused by Gram-positive bacteria, produces udder inflammation and oxidative injury in milk-producing mammals. Toll-like receptor 2(TLR2) is important for host recognition of invading Grampositive microbes. Over-expression of TLR2 in transgenic dairy goats is a useful model for studying various aspects of infection with Gram-positive bacteria, in vivo.Methods: We over-expressed TLR2 in transgenic dairy goats. Pam3CSK4, a component of Gram-positive bacteria,triggered the TLR2 signal pathway by stimulating the monocytes-macrophages from the TLR2-positive transgenic goats, and induced over-expression of activator protein-1(AP-1), phosphatidylinositol 3-kinase(PI3K) and transcription factor nuclear factor kappa B(NF-κB) and inflammation factors downstream of the signal pathway.Results: Compared with wild-type controls, measurements of various oxidative stress-related molecules showed that TLR2, when over-expressed in transgenic goat monocytes-macrophages, resulted in weak lipid damage, high level expression of anti-oxidative stress proteins, and significantly increased m RNA levels of transcription factor NF-E2-related factor-2(Nrf2) and the downstream gene, heme oxygenase-1(HO-1). When Pam3CSK4 was used to stimulate ear tissue in vivo the HO-1 protein of the transgenic goats had a relatively high expression level.Conclusions: The results indicate that the oxidative injury in goats over-expressing TLR2 was reduced following Pam3CSK4 stimulation. The underlying mechanism for this reduction was increased expression of the anti-oxidation gene HO-1 by activation of the Nrf2 signal pathway. | Shoulong Deng Kun Yu Wuqi Jiang Yan Li Sutian Wang Zhuo Deng Yuchang Yao Baolu Zhang Guoshi Liu Yixun Liu Zhengxing Lian | 2017 | Journal of Animal Science and Biotechnology2017，8，2： | 2 |
| 5 | Large-scale plasma grafts voltage stabilizer on hexagonal boron nitride for improving electrical insulation and thermal conductivity of epoxy composite显示文摘Better electrical insulation and thermal management are both urgently required in integrated power semiconductors.Electrical insulation epoxy encapsulation suffers from poor heat conduction,which has increasingly become a bottleneck of power semiconductors integration.Although incorporating high thermal conductivity ceramics,such as hexagonal boron nitride(hBN),aluminium nitride etc.into epoxy promotes the thermal conductivity,the eco-friendly scalable fabrication of these composites with sufficient electrical breakdown strength remains a formidable challenge.Suitable voltage stabilizers are known to provide additional benefits to breakdown strength.Herein,a highthroughput approach combining plasma with roll-to-roll was developed.The voltage stabilizer(acetophenone)was grafted on interfaces between hBN and epoxy matrix through plasma.The high-energy electrons are consumed by the grafted interface,which leads to the significant suppression of partial discharge in Epoxy/hBN.Meanwhile,interfacial phonon scattering is repaired by grafting.Therefore,the epoxy composite concurrently exhibits improved breakdown strength(by 27.4%)and thermal conductivity(by 142.9%)at about 11.9 wt.%filler content,outperforming the pure epoxy.Consequently,a promising modification strategy for mass production is provided for the encapsulation materials in various high-power-density semiconductor devices. | Penghao Zhang Chenguo Yao Liang Yu Xuetong Zhao Lisheng Zhao Linghan Lan Shoulong Dong | 2023 | High Voltage2023，8，3： | 2 |
| 6 | Efficient production of pronuclear embryos in breeding and nonbreeding season for generating transgenic sheep overexpressing TLR4显示文摘Background: Brucella is a zoonotic Gram-negative pathogen that causes abortion and infertility in ruminants and humans. TLR4 is the receptor for LPS which can recognize Brucella and initiate antigen-presenting cell activities that affect both innate and adaptive immunity. Consequently, transgenic sheep over-expressing TLR4 are an suitable model to investigate the effects of TLR4 on preventing Brucellosis. In this study, we generated transgenic sheep overexpressing TLR4 and aimed to evaluate the effects of different seasons(breeding and non-breeding season) on superovulation and the imported exogenous gene on growth.Results: In total of 43 donor ewes and 166 recipient ewes in breeding season, 37 donor ewes and 144 recipient ewes in non-breeding season were selected for super-ovulation and injected embryo transfer to generate transgenic sheep.Our results indicated the no. of embryos recovered of donors and the rate of pronuclear embryos did not show any significant difference between breeding and non-breeding seasons(P > 0.05). The positive rate of exogenous TLR4 tested were 21.21 % and 22.58 % in breeding and non-breeding season by Southern blot. The expression level of TLR4 in the transgenic sheep was 1.5 times higher than in the non-transgenic group(P < 0.05). The lambs overexpressing TLR4 had similar growth performance with non-transgenic lambs, and the blood physiological parameters of transgenic and non-transgenic were both in the normal range and did not show any difference.Conclusions: Here we establish an efficient platform for the production of transgenic sheep by the microinjection of pronuclear embryos during the whole year. The over-expression of TLR4 had no adverse effect on the growth of the sheep. | Yan Li Di Lian Shoulong Deng Xiaosheng Zhang Jinlong Zhang Wenting Li Hai Bai Zhixian Wang Hongping Wu Juncai Fu Hongbing Han Jianzhong Feng Guoshi Liu Ling Lian Zhengxing Lian | 2017 | Journal of Animal Science and Biotechnology2017，8，1： | 2 |
| 7 | Long-term field fertilization alters the diversity of autotrophic bacteria based on the ribulose-1,5-biphosphate carboxylase/oxygenase (RubisCO) large-subunit genes in paddy soil显示文摘 | Hongzhao Yuan Tida Ge Xiaohong Wu Shoulong Liu Chengli Tong Hongling Qin Minna Wu Wenxue Wei Jinshui Wu | 2012 | Applied Microbiology and Biotechnology2012，，4： | 1 |
| 8 | A novel high-voltage solid-state switch based on the SiC MOSFET series and its overcurrent protection显示文摘All-solid-state switches are one of the core components of pulsed power supply systems.However,the voltage level of a single switch is limited.By optimizing the chip structure,the voltage level of a single switch can be improved.Due to the immaturity of the production process and the positive correlation between the blocking voltage and the onresistance of the switch,it is difficult to improve the blocking voltage and the continuous forward current of a single switch simultaneously.The series-connected switch is a way to increase the switch's blocking voltage without reducing the switch's continuous forward current.Magnetically coupled isolated drive and resistor-capacitor forced voltage equalization techniques are investigated to increase the blocking voltage of the switches using multiple SiC metal-oxide-semiconductor field-effect transistors(MOSFETs)in series connections.Meanwhile,a special overcurrent protection scheme is designed to improve the reliability of the series-connected switch.Finally,a high-voltage solid-state switch is developed based on the SiC MOSFET series connections,whose output pulse width is adjustable from 20 to 300μs,frequency is adjustable from 1 Hz to 3 kHz,the maximum output voltage can reach 57 kV(1 Hz),and the overcurrent protection time is about 1μs. | Dazhao He Wenjie Sun Yixin Liao Penghao Zhang Liang Yu Shoulong Dong Chenguo Yao Xin liu | 2023 | High Voltage2023，8，4： | 1 |
| 9 | Development and Application of Decision Support Model for the Performance Optimization of Office Buildings Based on Grasshopper显示文摘With the expansion of the office building area,the energy consumption of office buildings is growing.High⁃performance building design contributes to energy saving and the development of green buildings.However,there is a lack of high⁃performance building tools and the workflow is often time⁃consuming.The building performance simulation,multiple objective optimizations,and the decision support model are the new approaches of high⁃performance building design.This paper proposes a newly developed decision support model,a high⁃performance building decision model named HPBuildingDSM,which integrates the building performance simulation,building performance multiple objective optimizations,building performance sampling,and parameter sensitivity analysis to design high⁃performance office buildings.In this research,the HPBuildingDSM was operated to search for the desirable office building design results with low⁃energy and high⁃quality daylighting performances.The simulated results had better daylighting performance and lower energy consumption,whose UDI100-2000 was 37.94%and annual energy consumption performance was 76.28 kWh/(m2·a),indicating a better building performance than the optimized results in the previous case study. | Hui Ren Shoulong Wang | 2021 | Journal of Harbin Institute of Technology(New Series)2021，28，4： | 0 |
| 10 | GPPre:A Python⁃Based Tool in Grasshopper for Office Building Performance Optimization显示文摘With the development of the economic and low⁃carbon society,high⁃performance building(HPB)design plays an increasingly important role in the architectural area.The performance of buildings usually includes the building energy consumption,building interior natural daylighting,building surface solar radiation,and so on.Building performance simulation(BPS)and multiple objective optimizations(MOO)are becoming the main methods for obtaining a high performance building in the design process.Correspondingly,the BPS and MOO are based on the parametric tools,like Grasshopper and Dynamo.However,these tools are lacking the data analysis module for designers to select the high⁃performance building more conveniently.This paper proposes a toolkit“GPPre”developed based on the Grasshopper platform and Python language.At the end of this paper,a case study was conducted to verify the function of GPPre,which shows that the combination of the sensitivity analysis(SA)and MOO module in the GPPre could aid architects to design the buildings with better performance. | Hui Ren Shoulong Wang | 2021 | Journal of Harbin Institute of Technology(New Series)2021，28，5： | 0 |
| 11 | Overexpression of Toll-like receptor 4 contributes to the internalization and elimination of Escherichia coli in sheep by enhancing caveolae-dependent endocytosis显示文摘Background:Gram-negative bacterial infections have a major economic impact on both the livestock industry and public health.Toll-like receptor 4(TLR4)plays a crucial role in host defence against Gram-negative bacteria.Exploring the defence mechanism regulated by TLR4 may provide new targets for treatment of inflammation and control of bacterial infections.In a previous study,we generated transgenic sheep overexpressing TLR4 by microinjection to improve disease resistance.The defence mechanism through which TLR4 overexpression protected these sheep against pathogens is still not fully understood.Results:In the present study,we used Escherichia coli to infect monocytes isolated from peripheral blood of the animal model.The overexpression of TLR4 strongly enhanced the percentage of endocytosis and capacity of elimination in monocytes during the early stages of infection.This phenomenon was mainly due to overexpression of TLR4 promoting caveolae-mediated endocytosis.Pretreatment of the transgenic sheep monocytes with inhibitors of TLR4,Src signalling,or the caveolae-mediated endocytosis pathway reduced the internalization of bacteria,weakened the ability of the monocytes to eliminate the bacteria,and increased the pH of the endosomes.Conclusion:Together,our results reveal the effects of TLR4 on the control of E.coli infection in the innate immunity of sheep and provide crucial evidence of the caveolae-mediated endocytosis pathway required for host resistance to invading bacteria in a large animal model,providing theoretical support for breeding disease resistance in the future.Furthermore,Src and caveolin 1(CAV1)could be potentially valuable targets for the control of infectious diseases. | Yao Li Yue Zhao Xueling Xu Rui Zhang Jinlong Zhang Xiaosheng Zhang Yan Li Shoulong Deng Zhengxing Lian | 2021 | Journal of Animal Science and Biotechnology2021，12，4： | 0 |
| 12 | Fast and Huge Anisotropic Diffusion of Cu(Ag) and Its Resistance on the Sn Self-diffusivity in Solid β–Sn显示文摘The site preferences,fast and huge anisotropic diffusion mechanisms of Cu(Ag) in β-Sn as well as their reduction on the self-diffusivity of Sn,have been investigated using the first-principles and ab initio molecular dynamics methods.We have found that Cu prefers the interstitial site,whereas Ag is preferable in the substitutional site,which is mostly dominated by their different size factors.Electronic structure further evidences that the d-s hybridization between the solute and the host atom also contributes to the site preferences.It is also deduced that the fast diffusion of Cu(Ag) is mostly due to the interstitial diffusion mechanism and their diffusivity can be correlated with the amount of their respective interstitial solution.Their faster diffusion along the c-axis can be attributed to the extremely low migration energy barrier caused by the straight tunnel of considerable size with the screw axis symmetry of 2π/4along the c-axis.Furthermore,it is found that during the process of diffusion the interstitially dissolved Cu(Ag) atoms would combine with the nearby Sn-vacancy and further annihilate the vacancy,thereby reducing the self-diffusion of Sn. | Peitao Liu Shoulong Wang Dianzhong Li Yiyi Li Xing-Qiu Chen | 2016 | Journal of Materials Science & Technology2016，32，2： | 0 |
| 13 | Effect of peroxiredoxin 6 on total and progressive motility of human spermatozoa after cryopreservation显示文摘Sperm cryopreservation is useful in assisted reproductive technology and male fertility preservation.However,freezing and thawing significantly reduces the total and progressive motility of human spermatozoa.In the present study,we explored the effects of peroxiredoxin 6(PRDX6)on total and progressive motility of human spermatozoa after cryopreservation.Semen samples of 20 males with normal parameters were collected and frozen in media supplemented with different concentrations of PRDX6(0 mM,10−5 mM,10−7 mM,and 10−9 mM,respectively).Postthaw total and progressive motility of sperms were measured.The results showed that in comparison with 0 mM,the concentrations of 10−5 mM,10−7 mM,and 10−9 mM of PRDX6 all significantly improved total motility and progressive motility of sperms(p<0.05).The 10−7 mM of PRDX6 showed the best performance.In conclusion,the supplementation of PRDX6 helps to maintain the total and progressive motility of human spermatozoa. | TIECHENG SUN LUYANG CHENG JING MA SHANJIE ZHOU YANDONG ZHANG WENDI ZHOU SHOULONG DENG SHUSONG WANG TIAN LI | 2020 | BIOCELL2020，44，3： | 0 |
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