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| 1 | A review on TiO_2 nanotube arrays:Fabrication, properties, and sensing applications显示文摘Fabrication, properties, and sensing applications of TiO2 nanotubes have been reviewed, and the highly ordered TiO2 nanotube arrays made by anodic oxidation in fluoride-contained electrolytes highlighted. The effect of anodization parameters (electrolyte, pH, and voltage) on the titania nanotube size and shape were discussed. The excellent biocompatibility of TiO2, the high orienta- tion, the large surface area with tunable pore sizes, as well as the high electron transfer rate along with the nanotubes make TiO2 nanotube array an ideal substrate for the sensor's fabrication and application. The sensors based on the TiO2 nanotube arrays for sensing hydrogen, oxygen, humidity, glucose and hydrogen peroxide all exhibited low detection limit, high stability, very good reproducibility and high sensitivity. | YANG LiXia LUO ShengLian CAI QingYun YAO ShouZhuo | 2010 | Chinese Science Bulletin2010，55，4： | 11 |
| 2 | The direct electrochemistry of glucose oxidase based on the synergic effect of amino acid ionic liquid and carbon nanotubes显示文摘Amino acid ionic liquids(AAILs) have attracted much attention due to their special chemical and physical properties,especially their outstanding biocompatibility and truly green aspect.In this work,a novel electrochemical biosensing platform based on AAILs/carbon nanotubes(CNTs) composite was fabricated.AAILs were used as a novel solvent for glucose oxidase(GOD) and the GOD-AAILs/CNTs/GC electrode was conveniently prepared by immersing the carbon nanotubes(CNTs) modified glassy carbon(GC) electrode into AAILs containing GOD.The direct electrochemistry of GOD on the GOD-AAILs/CNTs/GC electrode has been investigated and a pair of reversible peaks was obtained by cyclic voltammetry.The immobilized glucose oxidase could retain bioactivity and catalyze the reduction of dissolved oxygen.Due to the synergic effect of AAILs and CNTs,the GOD-AAILs/CNTs/GC electrode shows excellent electrocatalytic activity towards glucose with a linear range from 0.05 to 0.8 mM and a detection limit of 5.5 μM(S/N = 3).Furthermore,the biosensor exhibits good stability and ability to exclude the interference of commonly coexisting uric and ascorbic acid.Therefore,AAILs/CNTs composite can be a good candidate biocompatible material for the direct electrochemistry of the redox-active enzyme and the construction of third-generation enzyme sensors. | WANG MengDong DENG ChunYan NIE Zhou XU XiaHong YAO ShouZhuo | 2009 | Science China Chemistry2009，52，11： | 4 |
| 3 | Simultaneous determination of water-soluble and fat-soluble synthetic colorants in foodstuff by high-performance liquid chromatography–diode array detection–electrospray mass spectrometry显示文摘 | Ming Ma Xubiao Luo Bo Chen Shengpei Su Shouzhuo Yao | 2005 | Journal of Chromatography A2005，，1： | 2 |
| 4 | Electrochemical Sensor for Sensitive Determination of Ga(III) Ion Based on β-Cyclodextrin Incorporated Multi-walled Carbon Nanotubes and Imprinted Sol-gel Composite Film显示文摘为踪迹镓离子的察觉的一个新奇传感器[Ga (III )] 被一个金电极的逐步的修正与 -cyclodextrin 创造(-CD)/multi-walled 碳 nanotubes (MWCNT ) 和一个离子印了聚合物(IIP ) 。传感器表面形态学被扫描电子显微镜学描绘。印的传感器的电气化学的表演被周期的 voltammetry,微分脉搏 voltammetry 和 chronoamperometry 调查。传感器向目标 Ga (III ) 离子显示了优秀选择。同时,介绍 MWCNT 显示了显著催化活动,并且 CD 表明了重要丰富能力。一条线性刻度曲线被获得从 5.0 | Hu,Yufang Zhang,Zhaohui Zhang,Huabin Luo,Lijuan Zhang,Minglei Yang,Xiao Yao,Shouzhuo | 2012 | Chinese Journal of Chemistry2012，30，2： | 2 |
| 5 | Separation of 20(S)-protopanaxdiol type ginsenosides and 20(S)-protopanaxtrioltypeginsenosides with the help of macroporous resin adsorption and micro-wave assisted desorption显示文摘 | ZHAO YU CHEN BO YAO SHOUZHUO | 2007 | Separation and PurificationTechnology2007，52，3： | 1 |
| 6 | Amperometric Glucose Biosensor Based on Adsorption of Glucose Oxidase at Platinum Nanoparticle-Modified Carbon Nanotube Electrode显示文摘 | Tang Hao Chen Jinhua Yao Shouzhuo | 2004 | Anal Biochem2004，331，： | 1 |
| 7 | High-performance liquid chromatography electrospray ionization-mass spectrometry for simultaneous determination of taurine and 10 water-soluble vitamins in multivitamin tablets显示文摘 | CHEN Zhi CHEN Bo YAO Shouzhuo | 2006 | Analytica Chimica Acta2006，569，12： | 1 |
| 8 | Square wave anodic stripping voltammetric determination of lead(II) using a glassy carbon electrode modified with a lead ionophore and multiwalled carbon nanotubes显示文摘 | Xiuhui He Li Chen Xuan Xie Zhaohong Su Cong Qin Ying Liu Ming Ma Shouzhuo Yao Le Deng Qingji Xie Yun Tian Dilan Qin Yueping Luo | 2012 | Microchimica Acta (-)2012，，1： | 1 |
| 9 | Assembly of layer-by-layer films of superoxide dismutase and gold nanorods:A third generation biosensor for superoxide anion显示文摘Based on the layer-by-layer self-assembly of positively charged cetyltrimethylammonium bromide (CTAB) wrapped gold na- norods (AuNRs) and negatively charged superoxide dismutase (SOD) from their aqueous solutions on cysteine modified gold electrode (Cys/Au), a third generation electrochemical biosensor ((SOD/AuNRs)2/Cys/Au) for superoxide anion (O2·-) was developed. The two layers assembly of SOD/AuNRs can significantly enhance the direct electron transfer between SOD and the electrode. The functional enzymatic activities of the SOD offer an electrochemical approach to the determination of O2·-. In the reductive regions, the proposed sensor exhibits excellent analytical performances, such as wide linear range (200 nM to 0.2 mM O2·-), low detection limit (100 nM O2·-), high sensitivity (22.11 nA cm-2 μM-1), short response time (less than 5 s), good stability and reproducibility, while no obvious interferences are caused by commonly met interfering species including hydrogen peroxide (H2O2), uric acid (UA) and ascorbic acid (AA). | WANG MengDong HAN YiTao LIU XingXing NIE Zhou DENG ChunYan GUO ManLi YAO ShouZhuo | 2011 | Science China Chemistry2011，54，8： | 1 |
| 10 | DNA biosensor based on chitosan film doped with carbon nanotubes显示文摘 | Jia Li Qian Liu Yingju Liu Shanchao Liu Shouzhuo Yao | 2005 | Analytical Biochemistry2005，，1： | 1 |
| 11 | Application of ultrasonic technique for extracting chlorogenic acid from Eucommia ulmodies Oliv. ( E. ulmodies )显示文摘 | Hui Li Bo Chen Shouzhuo Yao | 2004 | Ultrasonics - Sonochemistry2004，，4： | 1 |
| 12 | Stability Analysis of an Underground Power Cavern in a Bedded Rock Formation显示文摘 | Yuli Xia Shouzhuo Peng Zhaoqi Gu | 2007 | Tunnelling and Underground Space Technology2007，22，2： | 1 |
| 13 | A sulpha - drug sensitive sensor based on ion - pair complex modified PQC resonator显示文摘 | Jinbin Yuan shouzhuo Yao | 2002 | Talanta2002，58，： | 1 |
| 14 | High-performanceliquid chromatography/eelectrospray ionization-mass spectrometry for simultaneous determination of taurine and 10 water-soluble vitamins in multivitamin tablets显示文摘 | Chen Zhi Chen Bo Yao Shouzhuo | 2006 | Analytica Chimica Acta2006，569，12： | 1 |
| 15 | A Series Piezoelectric Quartz Crystal Microbial Sensing TechniqueUsed for Biochemical Oxygen Demand Assayin Environmental Monitoring显示文摘 | Jinzhong Zhang Lili Bao Shouzhuo Yao | 1999 | Microchemical Journal1999，62，： | 1 |
| 16 | A goat-anti-human IgG modified piezoimmunosensor for Staphylococcus aureus detection显示文摘 | Deng Le Feng-jiang He Tai Jiao Jiang Lihua Nie Shouzhuo Yao | 1995 | Journal of Microbiological Methods1995，，2： | 1 |
| 17 | Slaration of 20 (S) -- proto- panaxdiol type ginsenosides and 20 (S)- protopanaxtriol type ginsenosides with the help of macroporous resin adsorption and microwave assisted desorption 显示文摘 | Yu Zhao Bo Chen Shouzhuo Yao | 2007 | Sep Purif Technol2007，，52： | 1 |
| 18 | Simultaneous direct analysis of benzimidazole fungicides and relevant metabolites in agricultural products based on multifunction dispersive solid-phase extraction and liquid chromatography–mass spectrometry显示文摘 | Bin Guo Zhiqiang Huang Meiling Wang Xiaoying Wang Ying Zhang Bo Chen Yongjun Li Hongfei Yan Shouzhuo Yao | 2010 | Journal of Chromatography A2010，，29： | 1 |
| 19 | Amperometric biosensor for NADH and ethanol based on electroreduced graphene oxide–polythionine nanocomposite film显示文摘 | Zou Li Yi Huang Li Chen Xiaoli Qin Zhao Huang Yaping Zhou Yue Meng Jing Li Siyu Huang Yan Liu Wen Wang Qingji Xie Shouzhuo Yao | 2013 | Sensors & Actuators: B Chemical2013，，： | 1 |
| 20 | Sensitive detection of DNA methyltransferase activity based on supercharged fluorescent protein and template-free DNA polymerization显示文摘DNA methylation, catalyzed by DNA methyltransferases(MTases), is a key component of genetic regulation, and DNA MTases have been regarded as potential targets in anticancer therapy. Herein, based on our previously developed DNA-mediated supercharged green fluorescent protein(Sc GFP)/graphene oxide(GO) interaction, coupled with methylation-initiated template-free DNA polymerization, we propose a novel fluorescence assay strategy for sensitive detection of DNA MTase activity. A hairpin DNA with a methylation-sensitive site and an amino-modified 3′-terminal(DNA-1) was designed and worked as a starting molecule. In the presence of DNA MTase, methylation-sensitive restriction endonuclease, and terminal deoxynucleotidyl transferase(Td T), DNA-1 can be sequentially methylated, cleaved, and further elongated. The resulting long DNA fragments quickly bind with Sc GFP and form the Sc GFP/DNA nanocomplex. Such nanocomplex can effectively protect Sc GFP from being adsorbed and quenched by GO. Without the methylation-initiated DNA polymerization, the fluorescence of Sc GFP will be quenched by GO. Thus, the DNA MTase activity, which is proportional to the amount of DNA polymerization products, can be measured by reading the fluorescence of Sc GFP/GO. The method was successfully used to detect the activity of DNA adenine methylation(Dam) MTase with a wide linear range(0.1–100 U/m L) and a low detection limit of 0.1 U/m L. In addition, the method showed high selectivity and the potential to be applied in a complex sample. Furthermore, this study was successfully extended to evaluate the inhibition effect of 5-fluorouracil on Dam MTase activity and detect Td T activity. | Daiqi Li Guoyan Lu Chunyang Lei Zhen Wang Lijun Li Zhou Nie Yan Huang Shouzhuo Yao | 2016 | Science China Chemistry2016，59，7： | 1 |
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