|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Mapping a mammalian adult adrenal gland hierarchy across species by microwell-seq显示文摘Recently,single-cell RNA-seq technologies have been rapidly updated,leading to a revolution in biology.We previously developed Microwell-seq,a cost-effective and high-throughput single cell RNA sequencing(scRNA-seq)method with a very simple device.Most cDNA libraries are sequenced using an expensive Illumina platform.Here,we present the first report showing combined Microwell-seq and BGI MGISEQ2000,a less expensive sequencing platform,to profile the whole transcriptome of 11,883 individual mouse adult adrenal gland cells and identify 18 transcriptionally distinct clusters.Moreover,we performed a single-cell comparative analysis of human and mouse adult adrenal glands to reveal the conserved genetic networks in these mammalian systems.These results provide new insights into the sophisticated adrenal gland hierarchy and provide a benchmark,low-cost strategy for highthroughput single-cell RNA study. | Shujing Lai Lifeng Ma Weigao E Fang Ye Haide Chen Xiaoping Han Guoji Guo | 2020 | Cell Regeneration2020，9，1： | 2 |
| 2 | A molecular roadmap for induced multi-lineage transdifferentiation of fibroblasts by chemical combinations显示文摘 | Xiaoping Han Hao Yu Daosheng Huang Yang Xu Assieh Saadatpour Xia Li Lengmei Wang Jie Yu Luca Pinello Shujing Lai Mengmeng Jiang Xueying Tian Fen Zhang Yanhong Cen Yuko Fujiwara Wei Zhu Bin Zhou Tianhua Zhou Hongwei Ouyang Jianan Wang Guo-Cheng Yuan Shumin Duan Smart H Orkin Guoji Guo | 2017 | Cell Research2017，27，3： | 2 |
| 3 | scRNA-seq profiling of neonatal and adult thymus-derived CD4+ T cells by a T cell origin-time tracing model显示文摘It is well documented that the neonatal thymus-derived (neonatal-TD) regulatory T cells (Treg) are essential to prevent lethal autoimmune diseases and allergies, and neonatal and adult thymus possesses distinct output potentials for naïve T cells, including Treg. However, the molecular features and detailed functional differences between neonatal-TD and adult thymus-derived (adult-TD) T cells in terms of their ability to maintain immune homeostasis during long-term environmental influences are still largely unknown, partially due to the lack of appropriate animal models to precisely trace these cells at specific time points. In this study, neonatal-TD and adult-TD CD4+ T cells from the spleen and Peyer's patches were traced for 9 weeks by a T cell origin-time tracing mouse model and analysed by single-cell RNA sequencing. More Treg but fewer naïve T cells were found in neonatal-TD CD4+ T cells from both tissues than those from adult-TD counterparts. Interestingly, the neonatal-TD Treg in both the spleen and Peyer's patches exhibited augmented expression of Foxp3, Gata3, Ctla4, Icos, Il2ra, Tgfb1, and Nrp1, as well as enriched Gene Ontology terms like T cell activation and tolerance induction, indicating an enhanced immunosuppressive function. These results were further confirmed by flow cytometry analysis and in vitro immune suppression assays. Flow cytometry also revealed a significantly higher proportion of neonatal-TD Treg in total Treg than that of adult-TD counterparts, suggesting the longer lifespan of neonatal-TD Treg. To investigate the intrinsic features of neonatal-TD and adult-TD CD4+ T cells, a shortened tracing time was performed. Surprisingly, the neonatal-TD and adult-TD CD4+ T cells had similar proportions of Treg and did not exhibit significant differences in Foxp3, Gata3, Ctla4, Icos, Il2ra, and Tgfb1 expression levels after tracing for 12 days. On the other hand, neonatal-TD Treg present an increased Nrp1 expression level compared with adult-TD counterparts, indicating the enhanced stability. Together, our work reveals that the neonatal-TD Treg are more immunosuppressive, which is likely shaped primarily by environmental factors. | Yuheng Han Xinxing Ouyang Yao Chen Shujing Lai Hongxiang Sun Ningbo Wu Chun Ruan Limin Lu Bing Su | 2022 | Journal of Molecular Cell Biology2022，14，12： | 0 |
| 4 | 利用微孔板测序技术绘制小鼠细胞图谱显示文摘文章简介长久以来,生命科学的研究主要基于群体细胞水平的分析,近几年所涌现的单细胞组学技术,使我们能够从单个细胞的水平上更为精确的解析细胞的分化、再生、衰老以及病变。在本研究中,科研团队自主开发了一套完全国产化的Microwell-seq高通量单细胞测序平台. | Xiaoping Han Renying Wang Yincong Zhou Lijiang Fei Huiyu Sun Shujing Lai Assieh Saadatpour Ziming Zhou Haide Chen Fang Ye Daosheng Huang Yang Xu Wentao Huang Mengmeng Jiang Xinyi Jiang Jie Mao Yao Chen Chenyu Lu Jin Xie Qun Fang Yibin Wang Rui Yue Tiefeng Li He Huang Stuart H.Orkin Guo-Cheng Yuan Ming Chen 郭国骥 | 2019 | 科学新闻2019，0，2： | 0 |
| 5 | Variability and Correlation of Egg Qualitative Characteristics in Ducks of Shaoxing Breed显示文摘Birds' selection on increase of egg's mass resulted in changes in the ratio of egg components. In this regard,now selection on improvement of eggs' quality became one of the most important directions of selection work. In this work,the interrelation among various qualitative characteristics of eggs of Shaoxing egg ducks breed,their variability and an individual assessment of ducks-mothers was considered. Prospects of carrying out selection on increase of yolk ratio in egg were estimated. | Lapa M.A Dmitriev V.B. Zeng Tao Lai Shujing He Li Li Liumen Lu Lizhi | 2015 | Animal Husbandry and Feed Science2015，7，4： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员