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| 1 | Reduced function and disassembled microtubules of cultured cardiomyocytes in spaceflight显示文摘Lack of gravity during spaceflight has profound effects on cardiovascular system, but little is known about how the cardiomyocytes respond to microgravity. In the present study, the effects of spaceflight on the structure and function of cultured cardiomyocytes were reported. The primary cultures of neo- natal rat cardiomyocytes were carried on Shenzhou-6 spacecraft and activated at 4 h in orbit. 8 samples were fixed respectively at 4, 48 and 96 h after launching for immunofluorescence of cytoskeleton, and 2 samples remained unfixed to analyze contractile and secretory functions of the cultures. Ground sam- ples were treated in our laboratory in parallel. After 115 h spaceflight, video recordings displayed that the number of spontaneous beating sites in flown samples decreased significantly, and the cells in the beating aggregate contracted in fast frequency without synchrony. Radioimmunoassay of the medium showed that the atrial natriuretic peptide secreted from flown cells reduced by 59.6%. Confocal images demonstrated the time-dependant disassembly of mirotubules versus unchanged distribution and or- ganization of microfilaments. In conclusion, above results indicate reduced function and disorganized cytoskeleton of cardiomyocytes in spaceflight, which might provide some cellular basis for further investigations to probe into the mechanisms underlying space cardiovascular dysfunction. | YANG Fen LI YingHui DING Bai NIE JieLin WANG HongHui ZHANG XiaoYou WANG ChunYan LING ShuKuan NI ChengZhi DAI ZhongQuan TAN YingJun WAN YuMin | 2008 | Chinese Science Bulletin2008，53，8： | 5 |
| 2 | NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly显示文摘The impairment of mitochondrial bioenergetics,often coupled with exaggerated reactive oxygen species (ROS) production, is a fundamental disease mechanism in organs with a high demand for energy, including the heart. Building a more robust and safer cellular powerhouse holds the promise for protecting these organs in stressful conditions. Here, we demonstrate that NADH:ubiquinone oxidoreductase subunit AB1 (NDUFAB1), also known as mitochondrial acyl carrier protein, acts as a powerful cardio-protector by conferring greater capacity and efficiency of mitochondrial energy metabolism. In particular, NDUFAB1 not only serves as a complex I subunit, but also coordinates the assembly of respiratory complexes I, II, and III, and supercomplexes, through regulating iron-sulfur biosynthesis and complex I subunit stability. Cardiac-specific deletion of Ndufab1 in mice caused defective bioenergetics and elevated ROS levels, leading to progressive dilated cardiomyopathy and eventual heart failure and sudden death. Overexpression of Ndufab1 effectively enhanced mitochondrial bioenergetics while limiting ROS production and protected the heart against ischemia-reperfusion injury. Together, our findings identify that NDUFAB1 is a crucial regulator of mitochondrial energy and ROS metabolism through coordinating the assembly of respiratory complexes and supercomplexes, and thus provide a potential therapeutic target for the prevention and treatment of heart failure. | Tingting Hou Rufeng Zhang Chongshu Jian Wanqiu Ding Yanru Wang Shukuan Ling Qi Ma Xinli Hu Heping Cheng Xianhua Wang | 2019 | Cell Research2019，29，9： | 4 |
| 3 | The mechanosensitive lncRNA Neat1 promotes osteoblast function through paraspeckle-dependent Smurf1 mRNA retention显示文摘Mechanical stimulation plays an important role in bone remodeling. Exercise-induced mechanical loading enhances bone strength,whereas mechanical unloading leads to bone loss. Increasing evidence has demonstrated that long noncoding RNAs(lnc RNAs) play key roles in diverse biological, physiological and pathological contexts. However, the roles of lnc RNAs in mechanotransduction and their relationships with bone formation remain unknown. In this study, we screened mechanosensing lnc RNAs in osteoblasts and identified Neat1, the most clearly decreased lnc RNA under simulated microgravity. Of note, not only Neat1 expression but also the specific paraspeckle structure formed by Neat1 was sensitive to different mechanical stimulations, which were closely associated with osteoblast function. Paraspeckles exhibited small punctate aggregates under simulated microgravity and elongated prolate or larger irregular structures under mechanical loading. Neat1 knockout mice displayed disrupted bone formation, impaired bone structure and strength, and reduced bone mass. Neat1 deficiency in osteoblasts reduced the response of osteoblasts to mechanical stimulation. In vivo, Neat1 knockout in mice weakened the bone phenotypes in response to mechanical loading and hindlimb unloading stimulation. Mechanistically, paraspeckles promoted nuclear retention of E3 ubiquitin ligase Smurf1 m RNA and downregulation of their translation, thus inhibiting ubiquitination-mediated degradation of the osteoblast master transcription factor Runx2, a Smurf1 target. Our study revealed that Neat1 plays an essential role in osteoblast function under mechanical stimulation, which provides a paradigm for the function of the lnc RNA-assembled structure in response to mechanical stimulation and offers a therapeutic strategy for long-term spaceflight-or bedrest-induced bone loss and age-related osteoporosis. | Caizhi Liu Xingcheng Gao Yuheng Li Weijia Sun Youjia Xu Yingjun Tan Ruikai Du Guohui Zhong Dingsheng Zhao Zizhong Liu Xiaoyan Jin Yinlong Zhao Yinbo Wang Xinxin Yuan Junjie Pan Guodong Yuan Youyou Li Wenjuan Xing Guanghan Kan Yanqing Wang Qi Li Xuan Han Jianwei Li Shukuan Ling Yingxian Li | 2022 | Bone Research2022，10，2： | 3 |
| 4 | Redox regulation of actin by thioredoxin-1 is mediated by the interaction of the proteins via cysteine 62显示文摘 | Xiaogang Wang Shukuan Ling Dingsheng Zhao | 2010 | Antioxidants & Redox Signaling2010，13，： | 1 |
| 5 | Electrochemi- cal synthesis of a ferrocenecarboxylic acid-C60 composite and its electrocatalysis to hydrogen peroxide显示文摘 | Miao Xiaopei Liu Lu Wang Shukuan | 2010 | Electro- chemistry Communications2010，12，： | 1 |
| 6 | Stackelberg game models between two competitive retailers in fuzzy decision environment显示文摘 | Shukuan Liu Zeshui Xu | 2014 | Fuzzy Optimization and Decision Making2014，，1： | 1 |
| 7 | The design of node operating system for cyber physical systems 显示文摘 | Du Xiaozhou Qiao Jianzhong Lin Shukuan | 2012 | Procedia Engineering2012，29，： | 1 |
| 8 | On the Existence of Positive Solutions for 2n-order Singular Boundary Value Problem显示文摘 | Liu Shukuan Du Xinsheng | 2007 | Journal of Qufu Normal University(Natural Science)2007，33，2： | 1 |
| 9 | PTEN Upregulation May Explain the Development of Insulin Resistance and Type 2 Diabetes with High Dose Statins显示文摘 | Yochai Birnbaum Manjyot K. Nanhwan Shukuan Ling Jose R. Perez-Polo Yumei Ye Mandeep Bajaj | 2014 | Cardiovascular Drugs and Therapy2014，，5： | 1 |
| 10 | Phosphodiesterase III Inhibition Increases cAMP Levels and Augments the Infarct Size Limiting Effect of a DPP-4 Inhibitor in Mice with Type-2 Diabetes Mellitus显示文摘 | Yochai Birnbaum Alexander C. Castillo Jinqiao Qian Shukuan Ling Hongmei Ye Jose R. Perez-Polo Mandeep Bajaj Yumei Ye | 2012 | Cardiovascular Drugs and Therapy2012，，6： | 1 |
| 11 | In vivo efficacy of recombinant methioninase is enhanced by the combination of polyethylene glycol conjugation and pyridoxal 5-phosphate supplementation显示文摘 | Sun Xinghua Yang Zhijian Li Shukuan | 2003 | Cancer Res2003，1，63： | 1 |
| 12 | HuR-mediated nucleocytoplasmic translocation of HOTAIR relieves its inhibition of osteogenic differentiation and promotes bone formation显示文摘Bone marrow mesenchymal stem cell(BMSC)osteogenic differentiation and osteoblast function play critical roles in bone formation,which is a highly regulated process.Long noncoding RNAs(lncRNAs)perform diverse functions in a variety of biological processes,including BMSC osteogenic differentiation.Although several studies have reported that HOX transcript antisense RNA(HOTAIR)is involved in BMSC osteogenic differentiation,its effect on bone formation in vivo remains unclear.Here,by constructing transgenic mice with BMSC(Prx1-HOTAIR)-and osteoblast(Bglap-HOTAIR)-specific overexpression of HOTAIR,we found that Prx1-HOTAIR and Bglap-HOTAIR transgenic mice show different bone phenotypes in vivo.Specifically,Prx1-HOTAIR mice showed delayed bone formation,while Bglap-HOTAIR mice showed increased bone formation.HOTAIR inhibits BMSC osteogenic differentiation but promotes osteoblast function in vitro.Furthermore,we identified that HOTAIR is mainly located in the nucleus of BMSCs and in the cytoplasm of osteoblasts.HOTAIR displays a nucleocytoplasmic translocation pattern during BMSC osteogenic differentiation.We first identified that the RNA-binding protein human antigen R(HuR)is responsible for HOTAIR nucleocytoplasmic translocation.HOTAIR is essential for osteoblast function,and cytoplasmic HOTAIR binds to miR-214 and acts as a ceRNA to increase Atf4 protein levels and osteoblast function.BglapHOTAIR mice,but not Prx1-HOTAIR mice,showed alleviation of bone loss induced by unloading.This study reveals the importance of temporal and spatial regulation of HOTAIR in BMSC osteogenic differentiation and bone formation,which provides new insights into precise regulation as a target for bone loss. | Yuheng Li Weijia Sun Jianwei Li Ruikai Du Wenjuan Xing Xinxin Yuan Guohui Zhong Dingsheng Zhao Zizhong Liu Xiaoyan Jin Junjie Pan Youyou Li Qi Li Guanghan Kan Xuan Han Shukuan Ling Xiqing Sun Yingxian Li | 2023 | Bone Research2023，11，4： | 0 |
| 13 | National Report on Space Medicine Progress in 2012–2014显示文摘The application of manned spaceflight makes a great impetus on the development of space medicine. With the deep growth of Chinese manned space, our national space medicine has attained great achievements. In this paper, the basic research of Space Medicine and its application in China during 2012–2014 were briefly reviewed. | LI Yinghui ZHANG Xiaoyou WAN Yumin DING Bai LIU Zhaoxia LING Shukuan BAI Yanqiang CHEN Shanguang LIU Shujuan | 2014 | 空间科学学报2014，34，5： | 0 |
| 14 | National Report on Space Medicine Progress in 2014-2016显示文摘The progress of China Spaceflight makes a great impetus for the development of space medicine.With the developing of China Space Station,the national space medicine has attained great achievements.In this paper,the basic research of Space Medicine and its application in China during 2014—2016 are briefly reviewed. | LI Yinghui ZHANG Xiaoyou WAN Yumin DING Bai LIU Zhaoxia LING Shukuan BAI Yanqiang CHEN Shanguang | 2016 | 空间科学学报2016，36，5： | 0 |
| 15 | Progress of Space Medicine Research in China显示文摘With the approaching of the Chinese Space Station(CSS)era,the focus of space medicine applications and related research has shifted to addressing the astronauts’health support in long-duration spaceflights,including nutrition,countermeasure against the physiological effects of weightlessness,medical monitoring and support,psychology status,etc.,and accordingly the human experiments to simulate long-duration weightlessness have been carried out.Increasingly,basic research has been put forward in the key areas,such as space bone loss,cardiovascular dysfunction and the molecular mechanisms underlying radiobiological effects.Moreover,specific novel research fields,such as hypometabolism technology,were explored.The research projects in the field of space medicine experiment,as an important aspect of the Chinese Space Station’s application,have been officially approved and launched. | DING Bai LIU Zhaoxia LÜKe LING Shukuan LIU Yue XU Zi LI Yinghui | 2020 | 空间科学学报2020，40，5： | 0 |
| 16 | Bacterial Cellulose-Based Janus Films with Radiative Cooling and Solar Heating Properties for All-Season Thermal Management显示文摘Advanced radiative cooling materials with both heating and cooling mode is of pivotal importance for all-season energy-saving in buildings.In this work,we report the design and fabrication of bacterial cellulose-based Janus films(J-BC)with radiative cooling and solar heating properties,which were developed by two-step vacuum-assisted filtration of modified MXene-doped bacterial cellulose and modified silicon nitride(Si_(3)N_(4))-doped bacterial cellulose,followed by hot-pressing and drying treatments.The as-prepared J-BC films show a unique Janus structure where modified MXene nanosheets and cellulose nanofibers are on the bottom surface,and modified silicon nitride(Si_(3)N_(4))nanoparticles and cellulose nanofibers are on the top surface.The radiative cooling effect of J-BC films is enabled by the Si_(3)N_(4)-doped bacterial cellulose due to the high mid-infrared emissivity of Si_(3)N_(4) nanoparticles,which shows a high solar reflection of~98.1%and high emissivity of~93.6%in the atmospheric transparency window(8-13μm).Thanks to the enhanced photothermal conversion of the modified MXene nanosheets,a reduced solar reflection(6.6%)and relatively low thermal emissivity in the atmospheric window(71.4%)are achieved,making sure the solar heating effect of J-BC films.In the outdoor tests,J-BC films achieve a sub-ambient temperature drop of~3.8°C and an above-ambient temperature rise of~14.2°C.Numerical prediction demonstrated that the J-BC films with dual modes have great potential of all-season energy saving for buildings and a corresponding energy-saving map in China is also created.The work disclosed herein can provide an avenue for the shaping of advanced radiative cooling materials for emerging applications of personal thermal management,sustainable energy-efficient buildings,and beyond. | Shukuan Shi Cristian Valenzuela Yanzhao Yang Yuan Liu Binxuan Li Ling Wang Wei Feng | 2023 | Chinese Journal of Chemistry2023，41，20： | 0 |
| 17 | LABEL-FREE DETECTION OF PROTEIN MICROARRAY WITH HIGH THROUGHPUT SURFACE PLASMON RESONANCE IMAGING(SPRI)显示文摘A surface plasmon resonance imaging(SPRI)system was developed for the discrimination of proteins on a gold surface.As a label-free and high-throughput technique,SPRI enables simultaneously monitoring of the biomolecular interactions at low concentrations.We used SPRI as a label-free and parallel method to detect different proteins based on protein microarray.Bovine Serum Albumin(BSA),Casein and Immunoglobulin G(IgG)were immobilized onto the Au surface of a gold-coated glass chip as spots forming a 6×6 matrix.These proteins can be discriminated directly by changing the incident angle of light.Excellent reproducibility for label-free detection of protein molecules was achieved.This SPRI platform represents a simple and robust method for performing high-sensitivity detection of protein microarray. | SHUKUAN XU GUOLIANG HUANG CHENG DENG JIANG ZHU CHAO HAN XIAOYONG YANG | 2008 | Journal of Innovative Optical Health Sciences2008，1，1： | 0 |
| 18 | Frequent 2-Episode Mining with Minimal Occurrences Based on Episode Matrix and Lock State显示文摘 | LIN Shukuan WANG Ya WANG Jue GUO Tianzhu QIAO Jianzhong | 2012 | Chinese Journal of Electronics2012，21，4： | 0 |
| 19 | DETECTION AND APPLICATION OF MICROFLUIDIC ISOTHERMAL AMPLIFICATION ON CHIP显示文摘Loop-mediated isothermal amplification(LAMP)is a novel nucleic acid amplification method.Compared with the widely utilized polymerase chain reaction(PCR),LAMP has higher speed and efficiency as well as lower requirement for system temperature control because the whole amplification process is isothermal and no efforts are needed to switch between different temperatures.In this paper,we designed and fabricated different kinds of polycarbonate(PC)microfluid chips,explored appropriate reaction condition for LAMP in microenvironment(1 nL→10μL),and developed a microfluidic isothermal amplification detection system.The DNA optimal amplification temperature is obtained;the starting time of exponential amplification of DNA is put forward farther.The optimal condition of DNA amplification in microenvironment,with a little reaction materials and early starting exponential amplification time of DNA are very important for clinic DNA detection and the application of Lab-on-a-Chip. | GUOLIANG HUANG XIAOYONG YANG JIANG ZHU SHUKUAN XU CHENG DENG CHAO HAN | 2008 | Journal of Innovative Optical Health Sciences2008，1，2： | 0 |
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