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| 1 | Wx^lv、the Ancestral Allele of Rice Waxy Gene显示文摘In rice grains,the Waxy (Wx) gene is responsible for the synthesis of amylose,the most important determinant for eating and cooking quality.The effects of several Wx alleles on amylose content and the taste of cooked rice have been elucidated.However,the relationship between artificial selection and the evolution of various Wx alleles as well as their distribution remain unclear.Here we report the identification of an ancestral allele,Wx^lv,which dramatically affects the mouthfeel of rice grains by modulating the size of amylose molecules.We demonstrated that WF originated directly from wild rice,and the three major Wx alleles in cultivated rice (Wx^b,Wx^a,and Wx^in) differentiated after the substitution of one base pair at the functional sites.These data indicate that the Wx^lv allele played an important role in artificial selection and domestication.The findings also shed light on the evolution of various Wx alleles,which have greatly contributed to improving the eating and cooking quality of rice. | Changquan Zhang Jihui Zhu Shengjie Chen Xiaolei Fan Qianfeng Li Yan Lu Min Wang Hengxiu Yu Chuandeng Yi Shuzhu Tang Minghong Gu Qiaoquan Liu | 2019 | Molecular Plant2019，12，8： | 22 |
| 2 | A rare Waxy allele coordinately improves rice eating and cooking quality and grain transparency显示文摘In rice(Oryza sativa), amylose content(AC) is the major factor that determines eating and cooking quality(ECQ). The diversity in AC is largely attributed to natural allelic variation at the Waxy(Wx)locus. Here we identified a rare Wx allele, Wx^(mw) ,which combines a favorable AC, improved ECQ and grain transparency. Based on a phylogenetic analysis of Wx genomic sequences from 370 rice accessions, we speculated that Wx^(mw) may have derived from recombination between two important natural Wx alleles, Wx^(in) and Wx^(b). We validated the effects of Wx^(mw) on rice grain quality using both transgenic lines and near-isogenic lines(NILs). When introgressed into the japonica Nipponbare(NIP) background, Wx^(mw) resulted in a moderate AC that was intermediate between that of NILs carrying the Wx^(b)allele and NILs with the Wx^(mp) allele. Notably, mature grains of NILs fixed for Wx^(mw) had an improved transparent endosperm relative to soft rice. Further, we introduced Wx^(mw) into a high-yielding japonica cultivar via molecular marker-assisted selection: the introgressed lines exhibited clear improvements in ECQ and endosperm transparency. Our results suggest that Wx^(mw) is a promising allele to improve grain quality, especially ECQ and grain transparency of high-yielding japonica cultivars, in rice breeding programs. | Changquan Zhang Yong Yang Shengjie Chen Xueju Liu Jihui Zhu Lihui Zhou Yan Lu Qianfeng Li Xiaolei Fan Shuzhu Tang Minghong Gu Qiaoquan Liu | 2021 | Journal of Integrative Plant Biology2021，63，5： | 18 |
| 3 | Development of gene-tagged molecular markers for starch synthesis-related genes in rice显示文摘Improving grain quality is an important goal in breeding new elite rice varieties,requiring effective tools for the identification of target genotypes.Molecular marker-aided selection(MAS),combined with conventional breeding approaches,enables us to pre-cisely identify the individual genotypes that are associated with different grain quality features,which can dramatically improve the breeding efficiency.However,to date,the number of molecular markers used in MAS for grain quality improvement is still somewhat limited.In this study,based on our previous study that rice grain quality is strongly associated with starch synthesis in the endosperm,we developed 51 gene-tagged molecular markers according to sequence variations in 18 starch synthesis-related genes from 16 typical rice cultivars.These markers can discriminate the different alleles among rice germplasms.These novel markers will provide effective tools in improving grain quality via the breeding new elite rice varieties. | TIAN ZhiXi YAN ChangJie QIAN Qian YAN Song XIE HuiLan WANG Fang XU JieFen LIU GuiFu WANG YongHong LIU QiaoQuan TANG ShuZhu LI JiaYang GU MingHong | 2010 | Chinese Science Bulletin2010，55，33： | 12 |
| 4 | Development and characterization of interspecific hybrids between Oryza sativa and O. latifolia by in situ hybridization显示文摘Oryza sativa and O. latifolia belong to the AA and CCDD genomes of Oryza, respectively. In this study, interspecific hybrids of these species were obtained using the embryo rescue technique. Hybrid panicle traits, such as long awns, small grain, exoteric large purple stigma, grain shattering and dispersed panicles, resemble that of the paternal parent, O. latifolia, whereas there is obvious heterosis in such respects as plant height, tillering ability and vegetative vigor. Chromosome pairing and the genomic components of the hybrid were subsequently investigated using genomic in situ hybridization (GISH) and fluorescent in situ hybridization (FISH) analysis. Based on the mitotic metaphase chromosome numbers of the root tips investigated, the hybrid is a triploid with 36 chromosomes. The genomic constitution of the hybrid is ACD. In the meiotic metaphase I of the hybrid pollen mother cell, poor chro- mosome pairing was identified and most of the chromosomes were univalent, which resulted in com- plete male sterility in the hybrid. | YI ChuanDeng TANG ShuZhu ZHOU Yong LIANG GuoHua GONG ZhiYun GU MingHong | 2008 | Chinese Science Bulletin2008，53，19： | 4 |
| 5 | Fine mapping and cloning of MT1,a novel allele of D10显示文摘瑞斯是为谷物生产的一个重要决定因素到 ering 为止。调查机制到 ering 为止,我们描绘了一到从装饰用的梨树变化识别的 ering 异种(mt1 ) 为止多重, Zhonghua 11,与他们对待。展览推进了到 ering 开发为止并且与野类型的植物相比相形见绌的这异种。印射的基因分析和好基因显示 mt1 异种被后退的基因控制,住在染色体 1 的 AP003376 上的一扇 29-kb 窗户上。在这个区域的一通常认为的基因,编码类胡萝卜素劈开 dioxygenase 8 (CCD8 ) ,对 D10 突变而产生之遗传。mt1 异种显型被野类型的 MT1 的介绍补充,并且在模仿的野类型的米饭的 MT1 击倒变异的显型。实时 PCR 分析显示 MT1 基因在腋的芽,圆锥花序,叶子,和根在茎并且在底层高度被表示。另外, MT1 表示清楚地在反馈规定下面。 | Yong Zhou Jinyan Zhu Zhengyi Li Fei Gu Honggen Zhang Shuzhu Tang Minghong Gu Guohua Liang | 2009 | Progress in Natural Science:Materials International2009，19，12： | 3 |
| 6 | Generation of selectable marker-free transgenic rice resistant to chewing insects using two co-transformation systems显示文摘生产可选没有标记(SMF ) 对咀嚼昆虫抵抗的转基因的米饭,杆菌 thuringiensis cryIA (c) 基因(Bt ) 被使用二个调停 Agrobacterium 的合作转变系统介绍进二个精英装饰用的梨树米饭变化。一个系统与在一 Agrobacterium 紧张的单个微型双胞胎 T-DNA 二进制向量,它由二个分开的 T-DNA 区域组成,当其它包含可选的标记基因时,带 Bt 的, hygromycin 抵抗基因(HPT ) 。另外的系统在二种分开的 Agrobacterium 文化使用二分开的二进制向量,包含单个原生质标志上的 Bt 或 HPT 基因。怀有 Bt 和可选的标记基因的很多独立转基因的米饭线被获得。结果证明 Bt 基因和 HPT 基因的合作转变频率由由二个分开的二进制向量系统(4.52%) 比那使用微型双胞胎 T-DNA 向量系统(29.87%) 是高得多的。然而,从 co 转基因的植物(21.74%) 的后代获得的 SMF 转基因的米饭植物的频率为后者比那为微型双胞胎 T-DNA 向量系统是更低的(50 – 60%) 。ELISA 的数据暗示表示 Bt 蛋白质作为 0.025 – 被测定; 0.103% 总数长叶在转基因的植物的可溶的蛋白质。因此,没有可选的标记基因,转基因的米饭衬里的几精英被选择。在 vitro 并且在 vivo 昆虫生物鉴定的从两个的结果显示 SMF 转基因的米饭被显示对有斑纹的茎 borer 和米饭叶文件夹高度抵抗。而且处于没有使用的任何杀虫药剂的一个自然的地条件,所有转基因的大米植物被发现到别被大米叶文件夹伤害,而野类型严重被损害。 | Hengxiu Yu Quanhong Yao Ling Wang Zhipeng Zhao Zhiyun Gong Shuzhu Tang Qiaoquan Liu Minghong Gu | 2009 | Progress in Natural Science:Materials International2009，19，11： | 2 |
| 7 | Molecular marker for screening rice cultivars with intermediate amylose content in indica显示文摘The waxy(Wx)gene of rice encodes the granule-bound starch synthase(GBSS),which was required in the synthesis of amylose in endosperm.Theamylose content(AC)of rice endosperm played an important role in grain yield,palatability,and processing quality.Our previous researehes showed that en-dosperm AC and GBSS contents were correlated with the ability of excising intron1 from the leader sequence of the Wx transcript.Cultivars with high | CAI Xiuling~(1)) LIU Qiaoquan~(2)) TANG Shuzhu~(2)) GU Minghong~(2)) WANG Zongyang~(1)) 1)Shanghai Inst of Plant Physiology,the Chinese Academy of Sciences,Shanghai 200032 2)College of Bioscienee and Biotechnology,Yangzhou Univ,Yangzhou 225009,China | 2002 | Chinese Rice Research Newsletter2002，10，3： | 1 |
| 8 | Stable Inheritance of the Antisense Waxy Gene in Transgenic Rice with Reduced Amylose Level and Improved Quality显示文摘 | Qiaoquan Liu Zongyang Wang Xiuhua Chen Xiuling Cai Shuzhu Tang Hengxiu Yu Jinliu Zhang Menming Hong Minghong Gu | 2003 | Transgenic Research2003，，1： | 1 |
| 9 | Stable Inheritance of the Antisense Waxy Gene in Transgenic Rice with Reduced Amylose Level and Improved Quality显示文摘 | Qiaoquan Liu Zongyang Wang Xiuhua Chen Xiuling Cai Shuzhu Tang Hengxiu Yu Jinliu Zhang Menming Hong Minghong Gu | 2003 | Transgenic Research2003，，1： | 1 |
| 10 | Identification and fine mapping of a fertility restorer gene for wild abortive cytoplasmic male sterility in the elite indica rice non-restorer line 9311显示文摘The wild abortive(WA)-type cytoplasmic male sterility(CMS)derived from the wild rice species Oryza rufipogon Griff.is used widely in three-line indica hybrids.The identification and mapping of restorer of fertility(Rf)genes aided in the development of WA-type hybrids.Here we report that testcross F1 plants from the WA-type CMS line and 9311 exhibited stainable pollen grains with no seed set,indicating that 9311 carries minor-effect Rfs for WA-type CMS.We developed an advanced backcross population consisting of plants harboring small regions of donor chromosomal segments from 9311 in the WATianfeng A genetic background with moderate seed setting rates.Genetic analysis showed that the pollen fertility levels of the backcross individuals are governed by a single gene from 9311 that we named Rf19(t).By use of the RICE 40 K gene chip,three introduced segments were identified in the fertile lines,and a candidate region spanning 4.37–8.29 Mb on chromosome 1 was identified for Rf19(t).Finally,Rf19(t)was fine-mapped to a region of 90 kb between the DNA marker loci STS1-163 and STS1-183,in which eight ORFs were predicted.Also,using relative expression analyses,comparative sequence analyses and functional domain analyses,we identified LOC_Os01g10530 as the most likely candidate gene for Rf19(t).Furthermore,Rf19(t)was found to function in fertility restoration,most probably by regulating the degradation of m RNA transcribed from the mitochondrial gene WA352.These results increase our knowledge of fertility restoration in WA-type CMS lines and will facilitate the development of high-quality pairs of WAtype CMS and maintainer lines. | Zuopeng Xu Yuanyue Du Xixu Li Ruixuan Wang Zhi'ai Chen Xiangqiang Zhao Qiaoquan Liu Shuzhu Tang Honggen Zhang | 2023 | The Crop Journal2023，11，3： | 0 |
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