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| 1 | Protective effect of essential oil of Pistacia atlantica Desf. on peptic ulcer: role of α-pinene显示文摘OBJECTIVE: To evaluate the efficacy of Pistacia atlantica Desf. oleoresin essential oil on peptic ulcer(PU) and its antibacterial effect on metronidazole-resistant Helicobacter pylori, as well as chemi-cal composition of the essential oil.METHODS: The essential oil was standardized using gas chromatography mass spectrometry(GC/MS) analysis. Acute toxicity of the essential oil was assessed in animal model. In vitro anti-Helicobacter pylori activity was performed through disc diffusion and minimum inhibitory concentration method. For gastroprotective assay, rats received Pistacia atlantica Desf. essential oil(25, 50 and 100 mg/kg orally) 1 h before induction of ulcer by ethanol.Macroscopic(ulcer index and protection rate) and microscopic examination were performed.RESULTS: The GC/MS analysis of the essential oil led to the identification of twenty constituents andα-pinene is predominant constituent. The essential oil was safe up to 2000 mg/kg. All Helicobacter pylori strains were susceptible to the essential oil and the MIC ranged from 275 to 1100 μg/m L. The ulcer index for treated groups was significantly reduced compared to control(P < 0.001) with EC50 value of 12.32 mg/kg. In microscopic examination, Pistacia atlantica attenuated destruction and necrosis of gastric tissue.CONCLUSION: Current study exhibited protective effect of standardized Pistacia atlantica essential oil against ethanol-induced gastric ulcer and its antibacterial activity on Helicobacter pylori. α-pinene might be the responsible agent. | Zahra Memariani Mohammad Sharifzadeh Mahbubeh Bozorgi Mannan Hajimahmoodi Mohammad Hosein Farzaei Mahdi Gholami Farideh Siavoshi Parastoo Saniee | 2017 | Journal of Traditional Chinese Medicine2017，37，1： | 10 |
| 2 | Reciprocal impact of host factors and Helicobacter pylori genotypes on gastric diseases显示文摘AIM: To assess the impact of Helicobacter pylori(H. pylori) genotypes and patient age and sex on the development of gastric diseases.METHODS: H. pylori-infected patients(n = 233) referred to the endoscopy unit at Tehran University of Medical Sciences(Tehran,Iran) were diagnosed with chronic gastritis(CG),gastric ulcer(GU),or duodenal ulcer(DU). Brucella blood agar was used for biopsy cultures and H. pylori isolation under microaerobic conditions. H. pylori isolates were confirmed with biochemical tests and through amplification of the 16 S r RNA gene. DNA was extracted from fresh cultures of the H. pylori isolates and used for amplification of vac A alleles and the cag A gene. Statistical analysis was performed to determine the association between H. pylori genotypes,age(< 40 years vs > 40 years) and sex of the patient,and gastric diseases.RESULTS: CG was the most prevalent gastric disease(113/233; 48.5%),compared to GU(64/233; 27.5%)and DU(56/233; 24%). More patients were male,and gastric diseases were more frequent in patients > 40 years(P < 0.05). The percentage of CG and GU patients that were male and female did not show a significant difference; however DU was more common in males(P < 0.05). Interestingly,a diagnosis of CG in patients > 40 years was more common in females(18.5%) than males(11.6%)(P = 0.05),whereas a diagnosis of GU or DU in patients > 40 years was more frequent in males(14.6% vs 10.7% and 12.4% vs 4.3%,respectively). Overall,genotyping of the H. pylori isolates revealed that the vac A s1(82%),vac A m2(70%),and cag A+(72.5%) alleles were more frequent than vac A s2(18%),vac A m1(29.2%),and cag A-(all P < 0.05). The vac A s1m2 cag A+ genotype was the most prevalent within the three disease groups. vac A s1m2 frequency was 56.2% with a similar occurrence in all diagnoses,while vac A s1m1 appeared more often in DU patients(33.9%). A genotype of vac A s2m2 occurred in 15% of isolates and was more common in CG patients(21.2%); vac A s2m1 was the least common genotype(3%). The vac A s1 allele was found to be a risk factor for DU,vac A s2 for CG,and vac A s1 and vac A s2 for GU(all P < 0.05). The vac A s2m2 genotype was associated with the development of CG and GU compared to DU(P < 0.05). No correlation was found between vac A m or cag A and gastric diseases.CONCLUSION: The outcome of H. pylori infection is the result of interaction between bacterial genotypes and the age and sex of infected individuals. | Sahar Honarmand-Jahromy Farideh Siavoshi Reza Malekzadeh Taher Nejad Sattari Saeid Latifi-Navid | 2015 | World Journal of Gastroenterology2015，21，31： | 5 |
| 3 | Vacuoles of Candida yeast as a specialized niche for Helicobacter pylori显示文摘Helicobacter pylori(H.pylori)are resistant to hostile gastric environments and antibiotic therapy,reflecting the possibility that they are protected by an ecological niche,such as inside the vacuoles of human epithelial and immune cells.Candida yeast may also provide such an alternative niche,as fluorescently labeled H.pylori were observed as fast-moving and viable bacterium-like bodies inside the vacuoles of gastric,oral,vaginal and foodborne Candida yeasts.In addition,H.pylori-specific genes and proteins were detected in samples extracted from these yeasts.The H.pylori present within these yeasts produce peroxiredoxin and thiol peroxidase,providing the ability to detoxify oxygen metabolites formed in immune cells.Furthermore,these bacteria produce urease and VacA,two virulence determinants of H.pylori that influence phago-lysosome fusion and bacterial survival in macrophages.Microscopic observations of H.pylori cells in new generations of yeasts along with amplification of H.pylori-specific genes from consecutive generations indicate that new yeasts can inherit the intracellular H.pylori as part of their vacuolar content.Accordingly,it is proposed that yeast vacuoles serve as a sophisticated niche that protects H.pylori against the environmental stresses and provides essential nutrients,including ergosterol,for its growth and multiplication.This intracellular establishment inside the yeast vacuole likely occurred long ago,leading to the adaptation of H.pylori to persist in phagocytic cells.The presence of these bacteria within yeasts,including foodborne yeasts,along with the vertical transmission of yeasts from mother to neonate,provide explanations for the persistence and propagation of H.pylori in the human population.This Topic Highlight reviews and discusses recent evidence regarding the evolutionary adaptation of H.pylori to thrive in host cell vacuoles. | Farideh Siavoshi Parastoo Saniee | 2014 | World Journal of Gastroenterology2014，20，18： | 3 |
| 4 | Yeast of the oral cavity is the reservoir of Heliobacter pylori显示文摘 | Salmaniam A H Siavoshi F AKbari | 2008 | J Oral Pathol Med2008，2，： | 1 |
| 5 | Detection of Helicobacter pylori-specifie genes in the oral yeast 显示文摘 | Siavoshi F Salmanian A H Akbari F | 2005 | Helicobacter2005，10，4： | 1 |
| 6 | Effect of contact force on breast tissue optical property measurements using a broadband diffuse optical spectroscopy handheld probe显示文摘 | Cerussi A Siavoshi A Durkin A | | 0，，21： | 1 |
| 7 | Antimicro bial effectiveness of furazolidone against metronidazole-resist ant strains of Helicobacter显示文摘 | Safaralizadeh R Siavoshi F Malekzadeh R | 2006 | East Mediterr Health J2006，12，34： | 1 |
| 8 | Yeast of the oral cavity is the reservoir of Heliobacter pylori 显示文摘 | Salmanian AH Siavoshi F Akbari F | 2008 | Oral Pathol Med2008，37，6： | 1 |
| 9 | Antimicro bial effectiveness of furazolidone against metronidazole-resist ant strains of Helicobacter显示文摘 | Safaralizadeh R Siavoshi F Malekzadeh R | 2006 | East Mediterr Health J2006，12，34： | 1 |
| 10 | Helicobacter pylori genotypes and types of gastritis in first-degree relatives of gastric cancer patients显示文摘 | Siavoshi F Asgharzadeh A Ghadiri H | 2011 | Int J Med Microbiol2011，301，60： | 1 |
| 11 | Immunodetection of Helicobacter pylori-specific Proteins in Oral and Gastric Candida Yeasts显示文摘 | Saniee P Siavoshi F Nikbakht Broujeni G | 2013 | Arch Iran Med2013，16，11： | 1 |
| 12 | Helicobacter pylori genotypes and types of gastritis in first-degree relatives of gastric cancer patients显示文摘 | Siavoshi F Asgharzadeh A Ghadiri H | 2011 | Int J Med Microbiol2011，301，6： | 1 |
| 13 | Association between Helicobacter pylori infection in gastric cancer, ulcers and gastritis in Iranian patients显示文摘 | Siavoshi F Malekzadeh R Daneshmand M | 2004 | Helieobacter2004，9，5： | 1 |
| 14 | Detection of Helieobacter pylori - specific genes in the oral yeast显示文摘 | Siavoshi F Salmanian AH Akbari F | 2005 | Helicobacter2005，10，4： | 1 |
| 15 | Yeast of the oralcavity is the reservoir of Heliobacter pylori显示文摘 | SALMANIAN A H SIAVOSHI F AKBARI F | 2008 | J Oral Pathol Med2008，，： | 1 |
| 16 | Candida accommodates non-culturable Helicobacter pylori in its vacuole-Koch's postulates aren't applicable显示文摘The following are the responses to the 'letter to the editor'('Helicobacter is preserved in yeast vacuoles! Does Koch's postulates confirm it?') authored by Nader Alipour and Nasrin Gaeini that rejected the methods, results, discussions and conclusions summarized in the review article authored by Siavoshi F and Saniee P. In the article, 7 papers, published between 1998 and 2013, were reviewed. The 7 papers had been reviewed and judged very carefully by the assigned expertise of the journals involved, including the reviewers of the World Journal of Gastroenterology(WJG), before publication. In the review article, 121 references were used to verify the methods, results and discussions of these 7 papers. The review article was edited by the trustworthy British editor of the(WJG), and the final version was rechecked and finally accepted by the reviewers of(WJG). None of the reviewers made comments like those in this 'letter to the editor', especially the humorous comments, which seem unprofessional and nonscientific. Above all, the authors' comments show a lack of understanding of basic and advanced microbiology, e.g. bacterial endosymbiosis in eukaryotic cells. Accordingly, their comments all through the letter contain misconceptions. The comments are mostly based on personal conclusions, without any scientific support. It would have been beneficial if the letter had been reviewed by the reviewers of the article by Siavoshi and Saniee. | Farideh Siavoshi Parastoo Saniee | 2018 | World Journal of Gastroenterology2018，24，2： | 0 |
| 17 | 幽门螺杆菌与胃病显示文摘H. pylori infection of the stomach is widespread among human populations including Iranians and is considered to play a major role in the pathogenesis of gastric diseases such as peptic ulcer, adenocarcinoma, and MALT lymphoma. The association between H. pylori virulence markers and disease has been studied in other populations around the world. The aim of this study was to investigate the distribution of H. pylori vacA and cagA genotypes and their association with clinical outcomes in Iran. H. pylori was cultured from gastric biopsy specimens obtained from 137 Iranian patients (58 with duodenal ulcer, 61 with nonulcer dyspeptic [NUD], and 18 with gastric adenocarcinoma). The vacA alleles and cagA genotypes were determined by PCR. The vacA sl allele was present in 107 of the 137 subjects (78%). Multiple strains (m1 and m2) were detected in H. pylori isolates from the patients. VacA s1 genotypes were more frequent in patients with peptic ulcer (46/58; 79%) than in NUD patients (47/61; 77%). CagA was present in 44%of the patients. NUD patients had a frequency of cagA positivity similar to that of the overall population (46%). CagA was present more frequently more than cagA-negative (20%vs. 8%, respectively) in patients with gastric carcinoma (20%) than cagA-negative in patients with gastric carcinoma (8%). This is the first comprehensive study to demonstrate the frequency of colonization with mixed strain, vacA s1, m1 and m2 as the dominant genotype in these Iranian patients, where a high rate of H. pylori infection exists and is similar to the region with a low rate of H. pylori infection. Therefore, host genetics, environmental factors, and the substantial genetic heterogeneity among different H. pylori strains may contribute to the different clinical outcomes. | Siavoshi F. Malekzadeh R. Daneshmand M. Ashktorab H. 赵天智 | 2006 | 世界核心医学期刊文摘(胃肠病学分册)2006，0，3： | 0 |
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