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| 1 | Developmental expression and stress induction of glutathione S -transferase in the spruce budworm, Choristoneura fumiferana显示文摘 | Qi-Li Feng Kenneth G Davey Anthony S.D. Pang Tim R Ladd Arthur Retnakaran Bill L Tomkins Sichun Zheng Subba R Palli | 2000 | Journal of Insect Physiology2000，，1： | 1 |
| 2 | Analysis of Offshore Oil Pipeline Floating Laying显示文摘-Based on the calculation model for the floating laying of the offshore oil pipeline, this paper analyses in detail the internal force, and deformation of the pipeline under a definite structural form (pipeline and buoy) and the way of pulling. The obtained results can be used for the buoy deployment, structure design, and the determination of pulling parameters (the pulling force of the cable and its length, etc.), providing an effective analysis method for floating pipeline-laying. A calculation example is given to show the related calculation process and the main results are analyzed and discussed. | Liang Zheng Lecture, Southwest Petroleum Institute, Nanchong 637001, Sichun Province | 1994 | China Ocean Engineering1994，9，1： | 0 |
| 3 | Cloning and expression of the nuclear transcription factor SIβFTZ-F1 cDNA from Spodoptera fitura显示文摘昆虫 molting 和变形被 20-hydroxyecdysone 调整,它激活蜕皮激素受体,由一系列抄写因素的表示列在后面。贝它 Fushi Tarazu 因素(FTZ-F1 ) 1 是这些蜕皮相关的早快车的抄写因素之一。FTZ-F1 互补 DNA (cDNA ) 从 Spodoptera litura 被克隆。全身的 cDNA 是在长度的 2 194 bp, SlFTZ-F1 cDNA 的开的读物框架由 1 659 bp 组成了并且与 61.4 kDa 的一个分子的团编码了 552-amino 酸蛋白质。相同分析表明那 SlFTZ-F1 蛋白质从另外的种类分享了高身份到它的相当或相同事物。A/B 域和 D 域在昆虫 FTZ-F1 蛋白质,而是 DNA 有约束力的域(C 域) 和 ligand 绑定域(E 域) 之中是多样的高度被保存。北污点分析在第五~第六个 molting 幼虫的外皮检测了 2.2 kb 的一个抄本。反向的抄写聚合酶链反应表明那 SlFTZ-F1 基因开始了到在每根脱毛前逐渐地并且周期性地快速,在 intermolt 阶段期间由减少列在后面。 | Lin Tang Chun Zhang Lin Liu Qili Feng Sichun Zheng | 2011 | Insect Science2011，18，6： | 0 |
| 4 | Alternative splicing of POUM2 regulates embryonic cuticular formation and tanning in Bombyx mori显示文摘Insect cuticle is an apical extracellular matrix produced by the epidermis,tracheal,hind-and foregut epithelia during embryogenesis and renewed during molting and metamorphosis.However,the underlying regulatory mechanism for embryonic cuticle formation remains largely unclear.Here,we investigate the function of the transcription factor POUM2 in the embryonic cuticular formation in Bombyx mori,a model lepidopteran insect.Clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein-9-mediated knockout of POUM2 resulted in the defect of cuticular deposition,pigmentation,and sclerotization in the embryos.Differentially expressed transcripts analysis of 7-d-old embryos identified 174 up-or downregulated cuticular protein transcripts,8 upregulated chitin degradation transcripts,2 downregulated chitin synthesis transcripts and 48 up-or downregulated transcription factor transcripts in the POUM2−/−embryos.The expression levels of the key factors of the tyrosine metabolic pathway,such as tyrosine hydroxylase(Th),Dopa decarboxylase(DDC),and arylalkylamine N-acetyltransferase(aaNAT),were significantly decreased in the POUM2−/−embryos.POUM2 isoform POUM2-L specifically bound the POU cis-regulatory element(CRE)in the Th promoter and increased the transcription of Th,whereas POUM2-S could not bind the POU CRE,although it also increased the transcription of Th.Heterogeneous nuclear ribonucleoprotein Squid-1 directly bound the POUM2 pre-mRNA(messenger RNA)and inhibited the alternative splicing of POUM2-L to POUM2-S mRNA.These results suggest that POUM2 participates in the cuticular formation by regulating the chitin and cuticular protein synthesis and metabolism,and the cuticular pigmentation and sclerotization by regulating tyrosine metabolism during embryogenesis.This study provides new insights into novel function of POUM2 in embryogenesis. | Chunmei Tong Kang Zhang Zixia Rong Wanyu Mo Yuling Peng Sichun Zheng Qi-Li Feng Huimin Deng | 2023 | Insect Science2023，30，5： | 0 |
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