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| 1 | Gene Identification and Expression Analysis of 86,136 Expressed Sequence Tags(EST)from the Rice Genome显示文摘Expressed Sequence Tag(EST)analysis has pioneered genome-wide gene discovery and expression profiling.In order to establish a gene expression index in the rice cultivar indica,we sequenced and analyzed 86,136 ESTs from nine rice cDNA libraries from the super hybrid cultivar LYP9 and its parental cultivars.We assembled these ESTs into 13,232 contigs and leave 8,976 singletons.Overall,7,497 sequences were found similar to the existing sequences in GenBank and 14,711 are novel.These sequences are classified by molecular function,biological process and pathways according to the Gene Ontology.We compared our sequenced ESTs with the publicly available 95,000 ESTs from japonica,and found little sequence variation,despite the large difference between genome sequences,We then assembled the combined 173,000 rice ESTs for further analysis.Using the pooled ESTs,we compared gene expression in metabolism pathway between rice and Arabidopsis according to KEGG.We further profiled gene expression patterns in different tissues,developmental stages,and in a conditional sterile mutant,after checking the libraries are comparable by means of sequence coverage.We also identified some possible library specific genes and a number of enzymes and transcription factors that contribute to rice development. | YanZhou JiabinTang MichaelG.Walker XiuqingZhang JunWang SongnianHu HuayongXu SigiLiu HuanmingYang JunYu JianWang YajunDeng JianhaiDong LinYe LiLin JunLi XuegangWang HaoHu YibinPan WeiLin WeiTian JingLiu LipingWei | 2003 | Genomics, Proteomics & Bioinformatics2003，1，1： | 4 |
| 2 | EST-based Analysis of Gene Expression in the Porcine Brain显示文摘Since pig is an important livestock species worldwide, its gene expression has been investigated intensively, but rarely in brain. In order to study gene expression profiles in the pig central nervous system, we sequenced and analyzed 43,122 highquality 5′ end expressed sequence tags (ESTs) from porcine cerebellum, cortex cerebrum, and brain stem cDNA libraries, involving several different prenatal and postnatal developmental stages. The initial ESTs were assembled into 16,101 clusters and compared to protein and nucleic acid databases in GenBank. Of these sequences, 30.6% clusters matched protein databases and represented function known sequences; 75.1% had significant hits to nucleic acid databases and partial represented known function; 73.3% matched known porcine ESTs; and 21.5% had no matches to any known sequences in GenBank. We used the categories defined by the Gene Ontology to survey gene expression in the porcine brain. | BingZhang WUJin YanwuZeng ZhixiSu SongnianHu JunYu | 2004 | Genomics, Proteomics & Bioinformatics2004，2，4： | 1 |
| 3 | Transcript profiles of Panax quinquefolius from flower, leaf and root bring new insights into genes related to ginsenosides biosynthesis and transcriptional regulation显示文摘 | QiongWu JingyuanSong YongqiaoSun FengmeiSuo ChenjiLi HongmeiLuo YingLiu YingLi XiaoweiZhang HuiYao XiwenLi SongnianHu ChaoSun | 2010 | Physiologia Plantarum2010，，2： | 1 |
| 4 | Gene Expression Profiling in Porcine Fetal Thymus显示文摘obtain an initial overview of gene diversity and expression pattern in porcinethymus, 11,712 ESTs (Expressed Sequence Tags) from 100-day-old porcine thymus(FTY) were sequenced and 7,071 cleaned ESTs were used for gene expressionanalysis. Clustered by the PHRAP program, 959 contigs and 3,074 singlets wereobtained. Blast search showed that 806 contigs and 1,669 singlets (totally 5,442ESTs) had homologues in GenBank and 1,629 ESTs were novel. According to theGene Ontology classification, 36.99% ESTs were cataloged into the gene expressiongroup, indicating that although the functional gene (18.78% in defense group) ofthymus is expressed in a certain degree, the 100-day-old porcine thymus still existsin a developmental stage. Comparative analysis showed that the gene expressionpattern of the 100-day-old porcine thymus is similar to that of the human infantthymus. | YanjiongChen ShengbinLi LinYe JianingGeng YajunDeng SongnianHu | 2003 | Genomics, Proteomics & Bioinformatics2003，1，2： | 1 |
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