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| 1 | Biochemical characters and antibiotic susceptibility of Staphylococcus aureus isolates显示文摘Objective:To observe the biochemical characters and antibiotic susceptibility of isolated Staphylococcia aureus(S.auerus) strains against some conventional and traditional antibiotics. Methods:Thirty post operative pathogenic isolated S.aureus strains were used in this study. Bacterial culture was done in Mueller-Hinton broth at 37 ℃.Characters of these strains were determined by traditional biochemical tests such as hydrolysis test of gelatin,urea,galactose, starch and protein,and fermentation of lactose and sucrose.Antibiotic susceptibility were carried out by minimum inhibilory concentration test,minium bactericidal concentration test,disc agar diffusion test and brain heart infusion oxacillin screening agar.Results:Prom this study,it was observed that 100%S.aureus isolates showed positive results in gelatin,urea and galactose hydrolysis test.50%isolates were positive in starch hydrolysis test,35%in protein hydrolysis test. 100%isolates in lactose fermenting test,but no isolate was positive in sucrose fermenting test. Antibiotic susceptibility testing suggested that 20%of isolates were resistant to kanamycin and 46.67%were resistant to oxacillin.Conclusions:These findings show that all these isolates have gelatin,urea,galactose hydrolysis and lactose fermenting activity.20%of these isolates were resistant to kanamvcin and 46.67%were resistant to oxacillin. | Subhankari Prasad Chakraborty Santanu Kar Mahapatra Somenath Roy | 2011 | Asian Pacific Journal of Tropical Biomedicine2011，1，3： | 5 |
| 2 | Nitric oxide mediated Staphylococcus aureus pathogenesis and protective role of nanoconjugated vancomycin显示文摘Objective:To investigate the relation between fruit seeds,plants residuals and appendicitis. Methods:Among cases that underwent appendectomy,the appendicitis cases having fruit seeds and undigested plant residuals in their etiology were examined retrospectively.Also, histopathological features,age,sex,and parameters of morbidity and mortality were used. Results:Fruit seed was found in one case(0.05%) with presence of pus in appendix lumen, undigested plant residuals in 7 cases(0.35%).It was determined that there were appendix inflammation in 2 of the plant residuals cases,while there were obstruction and lymphoid hyperplasia in the appendix lumen of 5 cases.No mortality was observed.Conclusions:The ratio of acute appendicitis caused by plants is minimal among all appendectomised patients, but avoidence of eating undigested fruit seeds and chewing plants well may help to prevent appendicitis. | Subhankari Prasad Chakraborty Santanu Kar Mahapatra Sumanta Kumar Sahu Sourav Chattopadhyay Panchanan Pramanik Somenath Roy | 2011 | Asian Pacific Journal of Tropical Biomedicine2011，1，2： | 4 |
| 3 | Antioxidative effect of folate-modified chitosan nanoparticles显示文摘Objective:To evaluate the potency of carboxymethyl chitosan-2,2' ethylenedioxy bisethylamine-folate(CMC-EDBE-FA) on tissue injury,antioxidant status and glutathione system in tissue mitochondria and serum against nicotine-induced oxidative stress in mice.Methods: CMC-EDBE-FA was prepared on basis of carboxymethyl chitosan tagged with folic acid by covalently linkage through 2,2' ethylenedioxy bis-ethylamine.Animals were divided into four groups,i.e.,control,nicotine(1 mg/kg bw/day),CMC-EDBE-FA(1 mg/kg bw/day) and nicotine(1 mg/kg bw/day) and CMC-EDBE-FA(1 mg/kg bw/day) for 7 days.Levels of lipid peroxidation, oxidized glutathione level,antioxidant enzyme status and DNA damage were observed and compared.Results:The significantly increase of lipid peroxidation,oxidized glutathione levels and DNA damage was observed in nicotine treated group as compared with control group;those were significantly reduced in CMC-EDBE-FA supplemented group.Moreover,significantly reduced antioxidant status in nicotine treated group was effectively ameliorated by the supplementation of CMC-EDBE-FA.Only CMC-EDBE-FA treated groups showed no significant change as compared with control group;rather than it repairs the tissue damage of nicotine treated group.Conclusions:These findings suggest that CMC-EDBE-FA is non-toxic and ameliorates nicotine-induced toxicity. | Subhankari Prasad Chakraborty Santanu Kar Mahapatra Sumanta Kumar Sahu Panchanan Pramanik Somenath Roy | 2011 | Asian Pacific Journal of Tropical Biomedicine2011，1，1： | 3 |
| 4 | Nanoconjugated vancomycin: new opportunities for the development of anti-VRSAagents显示文摘 | Subhankari Prasad Chakraborty Sumanta Kumar Sahu Santanu Kar Mahapatra Susmita Santra Manjusri Bal Somenath Roy Panchanan Pramanik | 2010 | Nanotechnology2010，，10： | 3 |
| 5 | Aqueous extract of Ocimum gratissimum Linn and ascorbic acid ameliorate nicotine-induced cellular damage in murine peritoneal macrophage显示文摘Objective:To test the in vitro protective role of aqueous extract of Ocimum gratissimum Linn. (0.gratissimum) and ascorbic acid against nicotine-induced murine peritoneal macrophage. Methods:Peritoneal macrophages from mice were treated with nicotine(10 mM),nicotine (10 mM) with aqueous extract of O.gratissimum(1 to 25μg/mL),and nicotine(10 mM) with ascorbic acid(0.01 mM) for 12 h in cell culture media,while the control group was treated with culture media.Levels of free radical generation,lipid peroxidation,protein carbonyls,oxidized glutathione levels and DNA damage were observed and compared.Results:Phytochemical analysis of aqueous extract has shown high amount of phenolics and flavonoids compound present in it.The significantly increased free radical generation,lipid peroxidation,protein carbonyls,oxidized glutathione levels and DNA damage were observed in nicotine-treated group as compared to the control group:those were significantly reduced in aqueous extract of O. gratissimum and ascorbic acid supplemented groups.Moreover,significantly reduced antioxidant status in nicotine exposed murine peritoneal macrophage was effectively ameliorated by these two products.Among the different concentration of aqueous extract of O.gratissimum,the maximum protective effect was observed at 10μg/mL which does not produce any significant change in the normal cell.Conclusions:These findings suggest the potential use and beneficial role of O.gratissimum as a modulator of nicotine-induced cellular damage in murine peritoneal macrophage. | Santanu Kar Mahapatra Subhankari Prasad Chakraborty Somenath Roy | 2010 | Asian Pacific Journal of Tropical Medicine2010，3，10： | 2 |
| 6 | Eugenol protects nicotine-induced superoxide mediated oxidative damage in murine peritoneal macrophages in vitro显示文摘 | Santanu Kar Mahapatra Subhankari Prasad Chakraborty Subrata Majumdar Braja Gopal Bag Somenath Roy | 2009 | European Journal of Pharmacology2009，，1： | 2 |
| 7 | Prevalence of severe chloroquine resistance associates the point mutation in pfcrt and pfmdrI gene in eastern India显示文摘 | Santanu Kar Mahapatra Subhankari Prasad Chakraborty Sabyasachi Das AmiyaKumar Hati Somenath Roy | 2011 | Asian Pacific Journal of Tropical Disease2011，，4： | 1 |
| 8 | Synthesis, characterization of chitosan–tripolyphosphate conjugated chloroquine nanoparticle and its in vivo anti-malarial efficacy against rodent parasite: A dose and duration dependent approach显示文摘 | Satyajit Tripathy Sabyasachi Das Subhankari Prasad Chakraborty Sumanta Kumar Sahu Panchanan Pramanik Somenath Roy | | International Journal of Pharmaceutics (-)0，，： | 1 |
| 9 | Alteration of some cellular function in amikacin resistant Pseudomonas aeruginosa transfected macrophages:a time dependent approach显示文摘Objective:To evaluate the free radical generation and antioxidant enzymes status in murine peritoneal macrophage during in vitro amikacin resistant Pseudomonas aeruginosa(ARPA) treatment with different time interval.Methods:Peritoneal macrophages were treated with 1× 10~2 CFU/mL ARPA cell suspension in vitro for different time interval(1,2,3,6,12,and 24 h) and super oxide anion generation,NO generation,reduced glutathione level and antioxidant enzymes status were analyzed.Results:Super oxide anion generation and NO generation got peak at 12 h,indicating maximal free radical generation through activation of NADPH oxidase in murine peritoneal macrophages during ARPA transfection.Reduced glutathione level and antioxidant enzymes status were decreased significantly(P<0.05) with increasing time of ARPA transfection. All the changes in peritoneal macrophages after 12 h in vitro ARPA transfection had significant difference(P<0.05).Conclusions:From this study,it may be summarized that in vitro ARPA infection not only generates excess free radical but also affects the antioxidant system and glutathione cycle in murine peritoneal macrophage. | Subhankari Prasad Chakraborty Santanu KarMahapatra Sabyasachi Das Somenath Roy | 2011 | Asian Pacific Journal of Tropical Biomedicine2011，1，6： | 1 |
| 10 | Methanol Extract of Ocimum Gratissimum Protects Murine Peritoneal Macrophages from Nicotine Toxicity By Decreasing Free Radical Generation, Lipid and Protein Damage and Enhances Antioxidant Protection显示文摘 | Santanu Kar Mahapatra Subhankari Prasad Chakraborty Subhasis Das Somenath Roy | 2009 | Oxidative Medicine and Cellular Longevity2009，，4： | 1 |
| 11 | Biocompatibility of folate-modified chitosan nanoparticles显示文摘Objective:To evaluate the acute toxicity of carboxymethyl chitosan-2,2' ethylenedioxy bisethylamine-folate(CMC-EDBE-FA) and as well as possible effect on microbial growth and in vitro cell cyto-toxicity.Methods:CMC-EDBE-FA was prepared on basis of carboxymethyl chitosan tagged with folic acid by covalently linkage through 2,2' elhylenedioxy bis-ethylamine. In vivo acute toxicity,in vitro cyto-toxicity and antimicrobial activity of CMC-EDBE-FA nanoparticle were determined.Results:Vancomycin exhibited the antibacterial activity against vancomycin sensitive Staphylococcus aureus,but CMC-EDBE-FA nanoparticle did not give any antibacterial activity as evidenced by minimal inhibitory concentration(MIC),minimal bactericidal concentration(MBC),disc agar diffusion(DAD) and killing kinetic assay.Further,the CMC-EDBE-FA nanoparticle showed no signs of in vivo acute toxicity up to a dose level of 1000 mg/kg p.o.,and as well as in vitro cyto-toxicity up to 230 μg/mL.Conclnsions:These findings suggest that CMC-EDBE-FA nanoparticle is expected to be safe for biomedical applications. | Subhankari Prasad Chakraborty Sumanta Kumar Sahu Panchanan Pramanik Somenath Roy | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，3： | 0 |
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