5篇
您的检索式：作者名="Suiqing Chen"
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| 1 | Pro-inflammatory cytokine network in peripheral inflammation response to cerebral ischemia显示文摘 | Lili Zeng Yongting Wang Jianrong Liu Ling Wang Suiqing Weng Kewei Chen Edward F. Domino Guo-Yuan Yang | 2013 | Neuroscience Letters2013，，： | 1 |
| 2 | Cocktail Blood Biomarkers: Prediction of Clinical Outcomes in Patients with Acute Ischemic Stroke显示文摘 | Zeng Lili Liu Jianrong Wang Yongting Wang Ling Weng Suiqing Chen Shengdi Yang Guo-yuan | 2013 | European Neurology2013，，2： | 1 |
| 3 | Pro-inflammatory cytokine network in peripheral inflammation response to cerebral ischemia显示文摘 | Lili Zeng Yongting Wang Jianrong Liu Ling Wang Suiqing Weng Kewei Chen Edward F. Domino Guo-Yuan Yang | 2013 | Neuroscience Letters2013，，： | 1 |
| 4 | Cocktail Blood Biomarkers: Prediction of Clinical Outcomes in Patients with Acute Ischemic Stroke显示文摘 | Zeng Lili Liu Jianrong Wang Yongting Wang Ling Weng Suiqing Chen Shengdi Yang Guo-yuan | 2013 | European Neurology2013，，2： | 1 |
| 5 | Identification of (-)-bornyl diphosphate synthase from Blumea balsamifera and its application for (-)-borneol biosynthesis in Saccharomyces cerevisiae显示文摘Borneol is a precious monoterpenoid with two chiral structures,(-)-borneol and(+)-borneol.Bornyl diphosphate synthase is the key enzyme in the borneol biosynthesis pathway.Many(+)-bornyl diphosphate synthases have been reported,but no(-)-bornyl diphosphate synthases have been identified.Blumea balsamifera leaves are rich in borneol,almost all of which is(-)-borneol.In this study,we identified a high-efficiency(-)-bornyl diphosphate synthase(BbTPS3)from B.balsamifera that converts geranyl diphosphate(GPP)to(-)-bornyl diphosphate,which is then converted to(-)-borneol after dephosphorylation in vitro.BbTPS3 exhibited a K m value of 4.93±1.38μM for GPP,and the corresponding k cat value was 1.49 s−1.Multiple strategies were applied to obtain a high-yielding(-)-borneol producing yeast strain.A codon-optimized BbTPS3 protein was introduced into the GPP high-yield strain MD,and the resulting MD-B1 strain produced 1.24 mg⋅L^(-1)(-)-borneol.After truncating the N-terminus of BbTPS3 and adding a Kozak sequence,the(-)-borneol yield was further improved by 4-fold to 4.87 mg⋅L^(-1).Moreover,the(-)-borneol yield was improved by expressing the fusion protein module of ERG20 F96W-N127W-YRSQI-t14-BbTPS3K2,resulting in a final yield of 12.68 mg⋅L^(-1) in shake flasks and 148.59 mg⋅L^(-1) in a 5-L bioreactor.This work is the first reported attempt to produce(-)-borneol by microbial fermentation. | Rui Ma Ping Su Qing Ma Juan Guo Suiqing Chen Baolong Jin Haiyan Zhang Jinfu Tang Tao Zhou Chenghong Xiao Guanghong Cui Luqi Huang | 2022 | Synthetic and Systems Biotechnology2022，7，1： | 0 |
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