| 1 | Micro-propagation of Lepedium meyenii Walp. (Maca) by shoot culture显示文摘The callus induction and plant regeneration system for an important plant, Lepedium meyenii Walp., has been established. Calli were induced from cotyledons petioles of Lepedium meyenii Walp within 4 weeks in a modified MS medium supplemented with BA plus NAA. The highest percentage of callus formation (57. 1% ) was found on MS medium supplemented with 0. 5 mg l^-1 BA and 0. 5 mg l^-1 NAA. During subculture on the shoot formation medium, most of calli proliferated and 50% -60% formed shoots. About 66.7% of shoots formed into roots on 1/2 strength MS containing 0.5mg l^-1 IBA after 4 weeks in culture. Chromosome count confirmed the number of the regeneration Maca plantlet was the same as that of the native plant(8x=64) .For regeneration of plantlets, from seedling via primary callus production, a four-step process of organogenes is required about 16 weeks. | ChengHua YuLongjiang HuQiongyue SunYouping JinWenwen ChenShancai LiMaoteng | 2004 | Agricultural Science & Technology2004，5，4： | 2 |
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