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| 1 | Gamma Irradiation of Embryogenic Callus Cultures and In vitro Selection for Salt Tolerance in Sugarcane (Saccharum officinarum L.)显示文摘Radiation induced mutagenesis followed by in vitro selection was employed for salt tolerance in popular Indian sugarcane (Saccharum officinarum L.) cv. CoC-671. Embryogenic calli were gamma irradiated and exposed to different levels of NaCl (42.8,85.6,128.3,171.1,213.9,256.7,299.5,or 342.2 mM). The relative growth rate (RGR) decreased progressively with increasing salt stress and was the least with a salt stress of 256.7 mM (0.25 ± 0.009),almost 10 fold lesser than the control. The RGR was significantly lower in 85.6 mM and higher salt stressed calli than the control. The survival percent also decreased,with an increase in NaCl concentration. In case of 10 and 20 Gy irradiated calli,regeneration was observed up to 85.6 mM NaCl selection,medium,whereas,higher treatments (128.3 mM and beyond) exhibited browning initially. However,in the subsequent subcultures,regeneration was obtained in the case of 10 and 20 Gy irradiated calli on 128.3 and 171.1 mM NaCl selections. Higher dose of gamma irradiation (40 Gy) also showed regeneration,but only with 85.6 mM NaCl selection. The unirradiated calli regenerated the highest number of plantlets followed by 10 and 20 Gy irradiated calli on salt selection. A total of 147 plantlets were selected from different salt levels. The salt selected plants are being tested for their field performance. | Vikas Y Patade P Suprasanna V A Bapat | 2008 | Agricultural Sciences in China2008，7，9： | 6 |
| 2 | Radiation-induced in vitro mutagenesis system for salt tolerance and other agronomic characters in sugarcane(Saccharum officinarum L.)显示文摘Gamma ray-induced in vitro mutagenesis and selection for salt(NaC l) tolerance were investigated in sugarcane(Saccharum officinarum L.). Embryogenic callus cultures were irradiated(10 to 80 Gy) and subjected to in vitro selection by exposure of irradiated callus to NaC l(0, 50, 100,150, 200, and 250 mmol L-1). Increasing NaC l concentrations resulted in growth reduction and increased membrane damage. Salt-selected callus lines were characterized by the accumulation of proline, glycine betaine, and Na+and K+concentration. Higher accumulation of proline and glycine betaine was observed in NaC l stressed callus irradiated at 20 Gy. Na+concentration increased and K+concentration decreased with increasing salt level. Irradiated callus showed50–60% regeneration under NaC l stress, and in vitro-regenerated plants were acclimatized in the greenhouse, with 80–85% survival. A total of 138 irradiated and salt-selected selections were grown to maturity and their agronomic performance was evaluated under normal and saline conditions. Of these, 18 mutant clones were characterized for different agro-morphological characters and some of the mutant clones exhibited improved sugar yield with increased Brix%,number of millable canes, and yield. The result suggest that radiation-induced mutagenesis offers an effective way to enhance genetic variation in sugarcane. | Ashok A.Nikam Rachayya M. Devarumath Akash Ahuja Harinath Babu Mahadeo G.Shitole Penna Suprasanna | 2015 | The Crop Journal2015，3，1： | 2 |
| 3 | Time course of physiological,biochemical,and gene expression changes under short-term salt stress in Brassica juncea L.显示文摘Salinity-imposed limitations on plant growth are manifested through osmotic and ionic imbalances. However, because salinity-induced responses vary considerably among crop plants, monitoring of such responses at an early stage has relevance. In this study,physiological(seed germination, seed vigor index, root length, shoot length, fresh weight,dry weight) and biochemical attributes(osmoprotectants, K^+/Na^+ ratio) were analyzed for a time-course assessment of salt responses in Indian mustard(Brassica juncea L.) with an emphasis on early monitoring. The results showed strong correlations for total soluble sugars at germination phase(24 h), proline content in the seedling establishment phase(48 h) and various physiological parameters including seed vigor index(R^2= 0.901), shoot length(R^2= 0.982), and fresh weight(R^2= 0.980) at 72 h(adaptation under stress). In addition, transcriptional changes were observed under NaCl treatment for key genes belonging to the family of selective ion transporters(NHX, HKT) and abscisic acid synthesis(AAO-3). The status of mitochondrial respiration was also examined as a probe for salinity tolerance at an early stage. The results suggested that although all the analyzed parameters showed correlations(negative or positive) with salt stress magnitude, their critical response times differed, with most of the studied biochemical, physiological, or molecular markers providing valuable information only after radicle emergence, whereas mitochondrial respiration via alternative oxidase was useful for the early detection of salt responses. | Manish Pandey Suprasanna Penna | 2017 | The Crop Journal2017，5，3： | 2 |
| 4 | Genome-wide analysis of thiourea-modulated salinity stress-responsive transcripts in seeds of Brassica juncea:Identification of signaling and effector components of stress tolerance显示文摘 | SRIVASTAVA AK RAMASWAMY NK SUPRASANNA P | 2010 | Annals of Botany2010，106，： | 1 |
| 5 | Somatic embyogenesis and plant regeneration from male flower buds in banana显示文摘 | Ganapathi TR Suprasanna P Bapat VA | 1999 | Curr Sci1999，76，9： | 1 |
| 6 | Developing somatic embryogenie culture system and plant regeneration in banana显示文摘 | Sidha M Suprasanna P Bapat V A | 2007 | Founder''s Day2007，285，： | 1 |
| 7 | Biochemical, physiological and growth changes in response to salinity in callus cultures of Sesuvium portulacastrum L.显示文摘 | Vinayak Haribhau Lokhande Tukaram Dayaram Nikam Suprasanna Penna | 2010 | Plant Cell Tissue and Organ Culture (PCTOC)2010，，1： | 1 |
| 8 | Phytofiltration of arsenic from simulated contaminated water using Hydrilla verticillata in field conditions 显示文摘 | Srivastava S Shrivastava M Suprasanna P | 2011 | Ecological Engineering2011，37，11： | 1 |
| 9 | Genome- wide analysis of thiourea- modulated salinity stress-responsive transcripts in seeds of Brassica juncea: identification of signalling and ef- lector components of stress tolerance 显示文摘 | Srivastava A K Ramaswamy N K Suprasanna P | 2010 | Annals of Botany2010，106，： | 1 |
| 10 | Simple and reproducible protocol for direct somatic embryogenesis from cultured immature inflorescence segments of sugarcane显示文摘 | DESAI N S SUPRASANNA P BAPAT V A | 2004 | Current Science2004，87，6： | 1 |
| 11 | Phytofil- tration of arsenic from simulated contaminated water using Hydrilla verticillata in field conditions显示文摘 | Srivastava S Shrivastava M Suprasanna P | 2011 | Ecological Engi- neering2011，37，11： | 1 |
| 12 | Differential gene expression in embryogenic, non-embryogenic and desiccation induced cultures of sugarcane显示文摘 | Suprasanna P Desai N S Nishanth G | 2004 | Sugar Technol2004，6，4： | 1 |
| 13 | An in vitro radiation induced mutagenesis-selection system for salinity tolerance in sug- arcane显示文摘 | Patade V Y Suprasanna P | 2009 | Sugar Tech2009，11，3： | 1 |
| 14 | Short-term salt and PEG stresses regulate expression of microRNA, miR159 in sugarcane leaves 显示文摘 | Patade V Y Suprasanna P | 2010 | J Crop Sci Biotech2010，13，3： | 1 |
| 15 | Somatic embryogenesis and plant regeneration from male flower buds in banana 显示文摘 | GANAPATHI TR SUPRASANNA P BAPAT V A | 1999 | Curt Sci1999，76，9： | 1 |
| 16 | Aroma synthesis in cell and callus cultures of rice显示文摘 | Suprasanna P Ganapathi T R Ramaswmy N K | 2000 | Rice Genet Newsl2000，15，： | 1 |
| 17 | Somatic embryogenesis and plant regeneration from male flower buds in banana显示文摘 | GANAPATHI T R SUPRASANNA P BAPAT V A | 1999 | Curr Sci1999，76，： | 1 |
| 18 | Aroma synthesis in cell and callus cultures of rice显示文摘 | Ganapathi TR Ramaswamy NK | 1998 | Rice Genet Newsl1998，15，： | 1 |
| 19 | Regeneration of plants from alginate-encapsulated somatic embryos of banana cv. Rasthali (Musa SPP. AAB Group)显示文摘 | T. R. Ganapathi L. Srinivas P. Suprasanna V. A. Bapat | | 0，，： | 1 |
| 20 | Genome-wide analysis of thiourea-rnodulated salinity stress-responsive transcripts in seeds of Brassica juncea: I- dentification of signaling and effector components of stress tol- erance 显示文摘 | SRIVASTAVA A K RAMASWAMY N K SUPRASANNA P | 2010 | Annals of Botany2010，106，： | 1 |
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