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| 1 | Existing drugs as broad-spectrum and potent inhibitors for Zika virus by targeting NS2B-NS3 interaction显示文摘Zika 病毒(ZIKV ) 的最近的爆发为治疗学加亮迫切需要。朊酶建筑群 NS2B-NS3 在 flaviviral polyprotein 处理期间起必要作用,并且因此代表一个吸引人的药目标。这里,我们开发了裂口酶识别直接指向 flavivirus NS2B-NS3 相互作用的 orthosteric 禁止者的基于互补的高产量的屏蔽试金。由屏蔽一个总数 2 816 同意了并且 investigational 药,我们识别了三个有势力候选人, temoporfin, niclosamide,和 nitazoxanide,,有 nanomolar 力量的 flavivirus NS2B-NS3 相互作用禁止者。显著地,在老鼠的在人的胎盘、神经的祖先房间的大多数有势力化合物, temoporfin,不是仅仅禁止的 ZIKV 复制,而且阻止的导致 ZIKV 的 viremia 和死亡当模特儿。结构的停靠建议 temoporfin 潜在地绑保持批评 NS2B 残余的 NS3 衣袋,因此禁止以一种非竞争的方式处理的 flaviviral polyprotein。当这些药已经在 USA 或另外的国家在另外的指示任何一个为临床的使用被同意了,他们由 ZIKV 和另外的 flaviviruses 为感染的管理代表有希望、容易开发的治疗。 | Zhong Li Matthew Brecher Yong-Qiang Deng Jing Zhang Srilatha Sakamuru Binbin Liu Ruili Huang Cheri A Koetzner Christina A Allen Susan A Jones Haiying Chen Na-Na Zhang Min Tian Fengshan Gao Qishan Lin Nilesh Banavali Jia Zhou Nathan Boles Menghang Xia Laura D Kramer Cheng-Feng Qin Hongmin Li | 2017 | Cell Research2017，27，8： | 11 |
| 2 | Development and Preliminary Evaluation of a Double-cell Ozonesonde显示文摘Ozonesondes are widely used to obtain ozone concentration profiles from the surface to the upper atmosphere. A kind of double-cell ozonesonde has been developed at the Institute of Atmospheric Physics(IAP), Chinese Academy of Sciences(named the 'IAP ozonesonde') based on previous experience over the past 20 years of developing the singlecell GPSO3 ozonesonde. The IAP ozonesonde is of the Electrochemical Concentration Cell(ECC) type. A detailed description of the IAP ozonesonde is firstly provided in the present paper, followed by a presentation of results from a series of launches carried out to evaluate its performance. The analysis involved comparing its observations with measurements from the GPSO3 and ECC ozonesondes(Model type ENSCI-Z) as well as a Brewer spectrophotometer. The results showed that the IAP ozonesonde is a vast improvement over the GPSO3 ozonesonde, able to capture vertical ozone structures very well and in good agreement with ECC ozonesonde measurements. The average difference in the ozone partial pressure between the IAP and ECC ozonesondes was 0.3 mPa from the surface to 2.5 km, close to zero from 2.5 to 9 km and generally less than 1 mPa for layers higher than 9 km. The apparent deviation is likely caused by a decreasing pump flow rate in the IAP ozonesonde which needs further improvement. The total ozone amounts measured by the IAP ozonesonde profiles were highly comparable with the Brewer data with a relative difference of 6%. The development of the IAP ozonesonde and its strong performance will surely accelerate the process of conventional observations of ozone profiles over China in the near future as well as provide more data for ozone research in general. | ZHANG Jinqiang XUAN Yuejian YAN Xiaolu LIU Mingyuan TIAN Hongmin XIA Xiang'ao PANG Li ZHENG Xiangdong | 2014 | Advances in Atmospheric Sciences2014，31，4： | 7 |
| 3 | Tailoring bimodal grain structure of Mg-9Al-1Zn alloy for strength-ductility synergy:Co-regulating effect from coarse Al_(2)Y and submicron Mg_(17)Al_(12) particles显示文摘Grain boundary strengthening is an effective strategy for increasing mechanical properties of Mg alloys.However,this method offers limited strengthening in bimodal grain-structured Mg alloys due to the difficultly in increasing the volume fraction of fine grains while keeping a small grain size.Herein,we show that the volume fraction of fine grains(FGs,~2.5μm)in the bimodal grain structure can be tailored from~30 vol.%in Mg-9 Al-1 Zn(AZ91)to~52 vol.%in AZ91-1Y(wt.%)processed by hard plate rolling(HPR).Moreover,a superior combination of a high ultimate tensile strength(~405 MPa)and decent uniform elongation(~9%)is achieved in present AZ91-1Y alloy.It reveals that a desired bimodal grain structure can be tailored by the co-regulating effect from coarse Al_(2)Y particles resulting in inhomogeneous recrystallization,and dispersed submicron Mg_(17)Al_(12)particles depressing the growth of recrystallized grains.The findings offer a valuable insight in tailoring bimodal grain-structured Mg alloys for optimized strength and ductility. | Yong-Kang Li Min Zha Hai-Long Jia Si-Qing Wang Hongmin Zhang Xiao Ma Teng Tian Pinkui Ma Hui-Yuan Wang | 2021 | Journal of Magnesium and Alloys2021，9，5： | 6 |
| 4 | An Adaptive PM Model Based on Difference Eigenvalue for Image Restoration显示文摘Classic Perona-Malik(PM) model is usually used to smooth noise for the degraded image. However, to our knowledge, a well-known defect of the PM model is prone to cause ‘staircase' effect and blur image fine feature because of the lack of correct diffusion strength in diffusion process. To tackle the problem, we will improve the PM model by developing a new structure descriptor to adjust anisotropic diffusion strength for smoothing noise and preserving image feature adaptively. The new structure descriptor is based on image local geometry-Hessian matrix, called difference eigenvalue, which can adaptively track edge feature and high degree of homogeneity in an image, even when the observed image is blurred. Experiments on both nature and medical images show that the improved PM method can achieve a superior performance than the traditional methods in terms of visual inspection and quantitative measurements. | TIAN Haiying CAI Hongmin LAI Jianhuang | 2014 | Chinese Journal of Electronics2014，23，4： | 3 |
| 5 | Discrimination of Chuanminshen violaceum Sheh et Shen from different regions based on fatty acid profiles of roots and leaves显示文摘Objectives:The purpose of this paper was to construct a reliable methodology to discriminate the geographical origins of Chuanminshen violaceum Sheh et Shan planted in different regions in Sichuan,China.Materials and methods:Fatty acid profiles of roots and leaves of C.violaceum planted in various regions of Sichuan Province in China,namely Guangyuan(GY),Langzhong(LZ),Jintang(JT),Bazhong(BZ),and Shuangling(SL),were determined using GC-MS followed by multivariate statistical analyses,including orthogonal partial least-squares discriminant analysis and hierarchical clustering analysis.Results:Leaves of C.violaceum showed the highest contents of hexadecatrienoic acid(3.21 g/kg),linoleic acid(6.62 g/kg),andα-linolenic acid(7.24 g/kg),which were all higher than those contained in roots.Chuanminshen violaceum samples collected from LZ,JT,and GY could be clearly distinguished based on fatty acid profiles of leaves and those collected from LZ,GY,and BZ could be clearly distinguished based on fatty acid profiles of roots.Conclusions:Chemometric method is used as a potential approach for analyses of fatty acid profiles of roots and leaves to control the quality of C.violaceum and their powered products. | Qing Zhang Junrui Tian Chong Wan Hongmin Dong Dingtao Wu Shuxiang Liu Wen Qin | 2020 | Food Quality and Safety2020，4，2： | 2 |
| 6 | Effect of UTRs from TMV-RNA on the expression of foreign gene in transgenic plants显示文摘To investigate the effects of 5’and 3’ untranslated region (UTR) from tobacco mosaicvirus (TMV) on expression of foreign genes, four plant-expression vectors pBG437, pBG438, pBG440 and pBG440ANOS containing expression cassettes of GUS-NOS; Ω-GUS-NOS, Ω-GUS-3’ UTR-NOS and Ω-GUS-3’ UTR downstream of CaMV 35S promoter with double enhancer sequences respectively have been constructed. Results from a large number of transgenic tobacco plants show that the GUS activity of pBG440 transformed plants is the highest, being 5-fold that of pBG437 and 1.6-fold that of pBG438. Similar results have been obtained in transient expression by injection of Agrobacterium tumefaciens harbouring these constructs into N. benthamiana leaves. These results obtained at the whole plant level confirm the conclusion drawn from the transient expression studies in protoplast system that TMV- Ω fragment can be a translation enhancer and the 3’ UTR has a coordinate effect with ft fragment to enhance foreign gene expression, but | Hongmin Qin Hongnian Guo Yantao Jia Lihong Li Yingchuan Tian | 2000 | Chinese Science Bulletin2000，45，18： | 2 |
| 7 | Ribonuclease H-like gene SMALL GRAIN2 regulates grain size in rice through brassinosteroid signaling pathway显示文摘Grain size is a key agronomic trait that determines the yield in plants.Regulation of grain size by brassinosteroids(BRs)in rice has been widely reported.However,the relationship between the BR signaling pathway and grain size still requires further study.Here,we isolated a rice mutant,named small grain2(sg2),which displayed smaller grain and a semi-dwarf phenotype.The decreased grain size was caused by repressed cell expansion in spikelet hulls of the sg2 mutant.Using map-based cloning combined with a Mut Map approach,we cloned SG2,which encodes a plant-specific protein with a ribonuclease H-like domain.SG2 is a positive regulator downstream of GLYCOGEN SYNTHASE KINASE2(GSK2)in response to BR signaling,and its mutation causes insensitivity to exogenous BR treatment.Genetical and biochemical analysis showed that GSK2 interacts with and phosphorylates SG2.We further found that BRs enhance the accumulation of SG2 in the nucleus,and subcellular distribution of SG2 is regulated by GSK2 kinase activity.In addition,Oryza sativa OVATE family protein 19(OsOFP19),a negative regulator of grain shape,interacts with SG2 and plays an antagonistic role with SG2 in controlling gene expression and grain size.Our results indicated that SG2 is a new component of GSK2-related BR signaling response and regulates grain size by interacting with Os OFP19. | Yunshuai Huang Hui Dong Changling Mou Ping Wang Qixian Hao Min Zhang Hongmin Wu Fulin Zhang Tengfei Ma Rong Miao Kai Fu Yaping Chen Ziyan Zhu Cheng Chen Qikai Tong Zhuoran Wang Shirong Zhou Xi Liu Shijia Liu Yunlu Tian Ling Jiang Jianmin Wan | 2022 | Journal of Integrative Plant Biology2022，64，10： | 1 |
| 8 | Construction of a new plant expression vector containing two insect resistant genes and its expression in transgenic tobacco plants显示文摘A new plant expression vector (pBS29K-BA) containing two insect resistant genes, a synthetic chimeric gene BtS29K encoding the activated insecticidal protein Cry1Ac and a gene API-BA encoding the arrowhead (Sagittaria sagittifolia L.) proteinase inhibitor (API) A and B, is constructed. Transgenic tobacco plants expressing these two genes are obtained through Agrobacterium-mediated transformation of tobacco leaf discs. The average expression levels of Cry1Ac and API-BA proteins in transgenic plants are of 3.2 μg and 4.9 μg per gram fresh leaf respectively. The results of insecticidal assay of transgenic plants indicate that the pBS29K-BA transformed plants are more resistant to insect damage than the plants expressing the Cry1Ac gene or API-BA gene alone. | GUO Hongnian, QIN Hongmin, CHEN Xiaoying, LI Changqing, LU Rui and TIAN Yingchuan(Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China) | 2002 | Progress in Natural Science:Materials International2002，12，5： | 1 |
| 9 | Romidepsin(FK228) improves the survival of allogeneic skin grafts through downregulating the production of donor-specific antibody via suppressing the IRE1α-XBP1 pathway显示文摘Antibody-mediated rejection(AMR)is one of the major causes of graft loss after transplantation.Recently,the regulation of B cell differentiation and the prevention of donor-specific antibody(DSA)production have gained increased attention in transplant research.Herein,we established a secondary allogeneic in vivo skin transplant model to study the effects of romidepsin(FK228)on DSA.The survival of grafted skins was monitored daily.The serum levels of DSA and the number of relevant immunocytes in the recipient spleens were evaluated by flow cytometry.Then,we isolated and purified B cells from B6mouse spleens in vitro by magnetic bead sorting.The B cells were cultured with interleukin-4(IL-4)and anti-clusters of differentiation 40(CD40)antibody with or without FK228 treatment.The immunoglobulin G1(IgG1)and IgM levels in the supernatant were evaluated by enzyme-linked immunosorbent assay(ELISA).Quantitative reverse transcription-polymerase chain reaction(RT-q PCR)and western blotting were conducted to determine the corresponding levels of messenger RNA(mRNA)and protein expression in cultured cells and the recipient spleens.The results showed that FK228 significantly improved the survival of allogeneic skin grafts.Moreover,FK228 inhibited DSA production in the serum along with the suppression of histone deacetylase 1(HADC1)and HDAC2 and the upregulation of the acetylation of histones H2A and H3.It also inhibited the differentiation of B cells to plasma cells,decreased the transcription of positive regulatory domain-containing 1(Prdm1)and X-box-binding protein 1(Xbp1),and decreased the expression of phosphorylated inositol-requiring enzyme 1α(p-IRE1α),XBP1,and B lymphocyte-induced maturation protein-1(Blimp-1).In conclusion,FK228 could decrease the production of antibodies by B cells via inhibition of the IRE1α-XBP1 signaling pathway.Thus,FK228 is considered as a promising therapeutic agent for the clinical treatment of AMR. | Yuliang GUO Siyu SONG Xiaoxiao DU Li TIAN Man ZHANG Hongmin ZHOU Zhonghua Klaus CHEN Sheng CHANG | 2022 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2022，23，5： | 0 |
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