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| 1 | Identification of Ca2+ signaling components in neural stem/progenitor cells during differentiation into neurons and glia in intact and dissociated zebrafish neurospheres显示文摘The development of the CNS in vertebrate embryos involves the generation of different sub-types of neurons and glia in a complex but highly-ordered spatio-temporal manner. Zebrafish are commonly used for exploring the development, plasticity and regeneration of the CNS, and the recent development of reliable protocols for isolating and culturing neural stem/progenitor cells(NSCs/NPCs) from the brain of adult fish now enables the exploration of mechanisms underlying the induction/specification/differentiation of these cells. Here, we refined a protocol to generate proliferating and differentiating neurospheres from the entire brain of adult zebrafish. We demonstrated via RT-qPCR that some isoforms of ip3 r, ryr and stim are upregulated/downregulated significantly in differentiating neurospheres, and via immunolabelling that 1,4,5-inositol trisphosphate receptor(IP3 R) type-1 and ryanodine receptor(RyR) type-2 are differentially expressed in cells with neuron-or radial glial-like properties. Furthermore, ATP but not caffeine(IP3 R and RyR agonists, respectively), induced the generation of Ca^(2+) transients in cells exhibiting neuron-or glial-like morphology. These results indicate the differential expression of components of the Ca^(2+) -signaling toolkit in proliferating and differentiating cells. Thus, given the complexity of the intact vertebrate brain, neurospheres might be a useful system for exploring neurodegenerative disease diagnosis protocols and drug development using Ca^(2+) signaling as a read-out. | Man Kit Tse Ting Shing Hung Ching Man Chan Tiffany Wong Mike Dorothea Catherine Leclerc Marc Moreau Andrew L.Miller Sarah E.Webb | 2018 | Science China(Life Sciences)2018，61，11： | 4 |
| 2 | Decomposition of Agregate Carbon Intensity for Freight Trends from 10OECD Countries for the Period 1971 -1993显示文摘 | Greening Loma A Ting Mike Davis William B | 1999 | Energy Economics1999，，4： | 1 |
| 3 | Enrichment of an anammox bacterial community from a flooded paddy soil显示文摘 | Bao‐lan Hu Li‐dong Shen Shuai Liu Chen Cai Ting‐ting Chen Boran Kartal Harry R. Harhangi Huub J. M. Op den Camp Li‐ping Lou Xiang‐yang Xu Ping Zheng Mike S. M. Jetten | 2013 | Environmental Microbiology Reports2013，，3： | 1 |
| 4 | Decomposition of Agregate Carbon Intensity for Freight Trends from 10OECD Countries for the Period 1971 -1993显示文摘 | Greening Loma A Ting Mike Davis William B | 1999 | Energy Economics1999，，4： | 1 |
| 5 | Decomposition of agregate carbon intensity for freight:trends from 10 OECD countries for the period 1971--1993 显示文摘 | Greening Lorna A Ting Mike Davis William B | 1999 | Energy Economics1999，，21： | 1 |
| 6 | In-situ gas contents of a multi-section coal seam in Sydney basin for coal and gas outburst management显示文摘The gas content is crucial for evaluating coal and gas outburst potential in underground coal mining. This study focuses on investigating the in-situ coal seam gas content and gas sorption capacity in a representative coal seam with multiple sections (A1, A2, and A3) in the Sydney basin, where the CO_(2) composition exceeds 90%. The fast direct desorption method and associated devices were described in detail and employed to measure the in-situ gas components (Q_(1), Q_(2), and Q_(3)) of the coal seam. The results show that in-situ total gas content (Q_(T)) ranges from 9.48 m^(3)/t for the A2 section to 14.80 m^(3)/t for the A3 section, surpassing the Level 2 outburst threshold limit value, thereby necessitating gas drainage measures. Among the gas components, Q_(2) demonstrates the highest contribution to Q_(T), ranging between 55% and 70%. Furthermore, high-pressure isothermal gas sorption experiments were conducted on coal samples from each seam section to explore their gas sorption capacity. The Langmuir model accurately characterizes CO_(2) sorption behavior, with ft coefcients (R^(2)) greater than 0.99. Strong positive correlations are observed between in-situ gas content and Langmuir volume, as well as between residual gas content (Q_(3)) and sorption hysteresis. Notably, the A3 seam section is proved to have a higher outburst propensity due to its higher Q_(1) and Q_(2) gas contents, lower sorption hysteresis, and reduced coal toughness f value. The insights derived from the study can contribute to the development of efective gas management strategies and enhance the safety and efciency of coal mining operations. | Zhongbei Li Ting Ren Dennis Black Ming Qiao Itmam Abedin Jessica Juric Mike Wang | 2023 | International Journal of Coal Science & Technology2023，10，4： | 0 |
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