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| 1 | Depletion of CD25^+CD4^+T cells (Tregs) enhances the HBV-specific CD8^+ T cell response primed by DNA immunization显示文摘AIM: Persistent hepatitis B virus (HBV) infection is characterized by a weak CD8+ T cell response to HBV. Immunotherapeutic strategies that overcome tolerance and boost these suboptimal responses may facilitate viral clearance in chronically infected individuals. Therefore, we examined whether CD25+CD4+ regulatory T (Treg) cells might be involved in a inhibition of CD8+T cell priming or in the modulation of the magnitude of the'peak' antiviral CD8+ T cell response primed by DNA immunization. METHODS: B10.D2 mice were immunized once with plasmid pCMV-S. Mice received 500 μg of anti-CD25 mAb injected intraperitoneally 3 d before DNA immunization to deplete CD25+ cells. Induction of HBV-specific CD8+ T ceils in peripheral blood mononuclear cells (PBMCs) was measured by S28-39 peptide loaded DimerX staining and their function was analyzed by intracellular IFN-γ staining.RESULTS: DNA immunization induced HBV-specific CD8+ T cells. At the peak T cell response (d 10), 7.1±2.0% of CD8+ T cells were HBV-specific after DNA immunization, whereas 12.7±3.2% of CD8+ T cells were HBV-specific in Treg-depleted mice, suggesting that DNA immunization induced more antigen-specific CD8+ T cells in the absence of CD25+ Treg cells (n = 6, P<0.05). Similarly, fewer HBVspecific memory T cells were detected in the presence of these cells (1.3±0.4%) in comparison to Treg-depleted mice (2.6±0.9%) on d 30 after DNA immunization (n = 6, P<0.01). Both IFN-γ production and the avidity of the HBV-specific CD8+ T cell response to antigen were higher in HBV-specific CD8+ T cells induced in the absence of Treg cells.CONCLUSION: CD25+ Treg cells suppress priming and/or expansion of antigen-specific CD8+ T cells during DNA immunization and the peak CD8+ T cell response is enhanced by depleting this cell population. Furthermore, Treg cells appear to be involved in the contraction phase of the CD8+ T ceil response and may affect the quality of memory T cell pools. The elimination of Treg cells or their inhibition may be important in immunotherapeutic strategies to control HBV infection by inducing virus-specific cytotoxic T lymphocyte responses in chronically infected subjects. | Yoshihiro Furuichi Hirotake Tokuyama Satoshi Ueha Makoto Kurachi Fuminori Moriyasu Kazuhiro Kakimi | 2005 | World Journal of Gastroenterology2005，11，24： | 30 |
| 2 | Differential regulation of elevated angiotensin Ⅱ in chronic renal ischemia显示文摘 | Hayashi K Matsuda H | 2002 | Hypertension2002，40，： | 1 |
| 3 | Melatonin at pharmaco- logical doses enhances human osteoblastic differentiation in vitro and promotes mouse cortical bone for mation in vivo 显示文摘 | Satomura K Tobiume S Tokuyama R | 2007 | J Pineal Res2007，42，3： | 1 |
| 4 | Acceleration of reaction by microwave irradiation显示文摘 | Tokuyama H Nakamura M | 2005 | Journal of Synthetic Chemistry Japan2005，63，5： | 1 |
| 5 | Small cell carcinoma of the gallbladder successfully treated by surgery and ad- juvant chemotherapy 显示文摘 | Imai H Matsui S Tokuyama Y | 2008 | Am Surg2008，74，3： | 1 |
| 6 | The Dipeptidyl Peptidase-4 Inhibitor Vildagliptin has the Capacity to Repair/3- Cell Dysfunction and Insulin Resistance显示文摘 | Horie A Tokuyama Y Ishizuka T | 2014 | Hormone and metabolic research : Hormon-und Stoffwechselforschung : Hormones et metabolisme2014，46，11： | 1 |
| 7 | The role of glutamate in the locus coeruleus during opioid withdrawal and effects of H - 7, a protein kinase inhibitor, on the action of glutamate in rats显示文摘 | Tokuyama S Zhu H Wakabayashi H | 1998 | J Biomed Sci1998，5，1： | 1 |
| 8 | A new method of estimating bronchial hyper-responsiveness in younger children 显示文摘 | Mochizuki H Mitsuhashi M Tokuyama K | 1985 | Ann Allergy1985，55，2： | 1 |
| 9 | Serum resistin level is associated with insulin sensitivity in Japanese patients with type 2 diabetes mellitus显示文摘 | Tokuyama Y Osawa H Ishizuka T | | 0，，05： | 1 |
| 10 | Quantitative approach to ultrasonic emulsion separation 显示文摘 | NII S KIKUMOTO S TOKUYAMA H | 2009 | Ultrasonics Sonochemistry2009，16，1： | 1 |
| 11 | C]inical evaluation of autologous gamma delta T cell-basedimmunotherapy for metastatic solid tumours显示文摘 | Nicol AJ Tokuyama H Mattarollo 81{ | 2011 | British J Oancer2011，6，105： | 1 |
| 12 | Novel synthesis of maeroporous poly(N-isopropylacrylarnide) hydrogels using oil-in-water emulsions显示文摘 | Tokuyama H Kanehara A | 2007 | Langmuir2007，23，11： | 1 |
| 13 | Childhood cough variant asthma and its relationship to classic asthma显示文摘 | TODOKORO M MOCHIZUKI H TOKUYAMA K | 2003 | Ann Allergy Asthma Immunol2003，90，： | 1 |
| 14 | Ornithobacterium rhinotracheale infection in Japan : preliminary inverstigations 显示文摘 | Sakai E Tokuyama Y Nonaka F | 2002 | Vet Rec2002，46，17： | 1 |
| 15 | Anaphylaxis to grand keyhole limpet( abalone- like shellfish)and abalone显示文摘 | Morikawa A Kato M Tokuyama K | 1990 | Ann Al- lergy1990，65，5： | 1 |
| 16 | Solid-phase extraction of indium( Ⅲ ) ions onto thermosensitive poly(N-isopropylacrylamide) 显示文摘 | Tokuyama H Iwama T | 2009 | Separation and Purification Technology2009，68，3： | 1 |
| 17 | Characteristic features of al- lergic airway inflammation in a murine model of infantile asthma 显示文摘 | Ohki Y Tokuyama K Mayuzumi H | 2005 | Int Arch Allergy Immunol2005，138，1： | 1 |
| 18 | Clinical evaluation of autologous gamma delta T cell-based immunotherapy for metastatic solid tumours显示文摘 | Nicol AJ Tokuyama H Mattarollo SR | 2011 | Br J Cancer2011，105，6： | 1 |
| 19 | Bronchial sensitivity and bronchial reactivity in children with cough variant asthma显示文摘 | Mochizuki H Arakawa H Tokuyama K | 2005 | Chest2005，128，4： | 1 |
| 20 | Ileal perforation in a patient with high spinal cord injury: report of a case 显示文摘 | Matsuo A Tokuyama Y Hosono Y | 2004 | Surg Today2004，34，1： | 1 |
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