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| 1 | Intestinal alkaline phosphatase in the colonic mucosa of children with inflammatory bowel disease显示文摘AIM:To investigate intestinal alkaline phosphatase(iAP) in the intestinal mucosa of children with inflammatory bowel disease(IBD).METHODS:Colonic biopsy samples were taken from 15 newly diagnosed IBD patients and from 10 healthy controls.In IBD patients,specimens were obtainedboth from inflamed and non-inflamed areas.The iAP mRNA and protein expression was determined by reverse transcription-polymerase chain reaction and Western blotting analysis,respectively.Tissue localization of iAP and Toll-like receptor(TLR) 4 was investigated by immunofluorescent staining.RESULTS:The iAP protein level in the inflamed mucosa of children with Crohn's disease(CD) and ulcerative colitis(UC) was significantly decreased when compared with controls(both P < 0.05).Similarly,we found a significantly decreased level of iAP protein in the inflamed mucosa in CD compared with non-inflamed mucosa in CD(P < 0.05).In addition,the iAP protein level in inflamed colonic mucosa in patients with UC was decreased compared with non-inflamed mucosa in patients with CD(P < 0.05).iAP protein levels in the non-inflamed mucosa of patients with CD were similar to controls.iAP mRNA expression in inflamed colonic mucosa of children with CD and UC was not significantly different from that in non-inflamed colonic mucosa with CD.Expression of iAP mRNA in patients with noninflamed mucosa and in controls were similar.Co-localization of iAP with TLR4 showed intense staining with a dotted-like pattern.iAP was present in the inflamed and non-inflamed mucosa of patients with CD,UC,and in control biopsy specimens,irrespective of whether it was present in the terminal ileum or in the colon.However,the fluorescent signal of TLR4 was more pronounced in the colon compared with the terminal ileum in all groups studied.CONCLUSION:Lower than normal iAP protein levels in inflamed mucosa of IBD patients may indicate a role for iAP in inflammatory lesions in IBD.Based on our results,administration of exogenous iAP enzyme to patients with the active form of IBD may be a therapeutic option. | Kriszta Molnár dám Vannay Beáta Szebeni Nóra Fanni Bánki Erna Sziksz ron Cseh Hajnalka Gyrffy Péter László Lakatos Mária Papp András Arató Gábor Veres | 2012 | World Journal of Gastroenterology2012，18，25： | 5 |
| 2 | Increased duodenal expression of mi R-146a and-155 in pediatric Crohn's disease显示文摘AIM: To evaluate the role of micro RNA(mi R)-146 a,-155 and-122 in the duodenal mucosa of pediatric patients with Crohn's disease(CD) and the effect of transforming growth factor-β(TGF-β) on these mi Rs in duodenal epithelial and fibroblast cells.METHODS: Formalin-fixed, paraffin-embedded biopsies derived from the macroscopically inflamed(CD inflamed: n = 10) and intact(CD intact: n = 10) duodenal mucosa of pediatric CD patients and control children(C: n = 10) were examined. Expression of mi R-146 a,-155 and-122 was determined by realtime polymerase-chain reaction(PCR). The expression of the above mi Rs was investigated in recombinant human TGF-β(1 nmol/L, 24 h) or vehicle treated small intestinal epithelial cells(CCL-241) and primary duodenal fibroblast cells derived from healthy children as well.RESULTS: Expression of mi R-146 a was significantly higher in the inflamed duodenal mucosa compared to the intact duodenal mucosa of children with CD(CD inflamed: 3.21 ± 0.50 vs CD intact: 0.62 ± 0.26, p ≤ 0.01) and to the control group(CD inflamed: 3.21 ± 0.50 vs C: 1.00 ± 0.33, p ≤ 0.05). The expression of mi R-155 was significantly increased in the inflamed region of the duodenum compared to the control group(CD inflamed: 4.87 ± 1.02 vs Control: 1.00 ± 0.40, p ≤ 0.001). The expression of mi R-122 was unchanged in the inflamed or intact mucosa of CD patients compared to controls. TGF-β treatment significantly decreased the expression of mi R-155 in small intestinal epithelial cells(TGF-β: 0.7 ± 0.083 vs Control: 1 ± 0.09, p ≤ 0.05) and also the expression of mi R-146a(TGF-β: 0.67 ± 0.04 vs Control: 1 ± 0.15, p ≤ 0.01) and mi R-155(TGF-β: 0.72 ± 0.09 vs Control: 1 ± 0.06, p ≤ 0.05) in primary duodenal fibroblasts compared to corresponding vehicle treated controls. TGF-β treatment did not influence the expression of mi R-122.CONCLUSION: The elevated expression of mi R-146 a and-155 in the inflamed duodenal mucosa of CD patients suggests the role of these mi Rs in the pathomechanism of inflammatory bowel disease. Antiinflammatory TGF-β plays an important role in the regulation of the expression of these mi Rs. | Dániel Szucs Nóra Judit Béres Réka Rokonay Kriszta Boros Katalin Borka Zoltán Kiss András Arató Attila J Szabó ádám Vannay Erna Sziksz Csaba Bereczki Gábor Veres | 2016 | World Journal of Gastroenterology2016，22，26： | 2 |
| 3 | Divergence of renal vascular endothelial growth factor mRNA expression and protein level in post- ischaemic rat kidneys 显示文摘 | Vannay A Fekete A dori C | 2004 | Experimental Physiology2004，89，4： | 1 |
| 4 | Genetic polymorphisms of vascular endothelial growth factor in severe pre--eclarnpsia显示文摘 | Banyasz I Szabo S Bokodi G Vannay A Vasarhelyi B Szabo A | 2006 | Mol Hum Reprod2006，12，： | 1 |
| 5 | Protective effects of hypercapnic acidosis on ventilator - induced lung injury显示文摘 | Broccard AF Hotchkiss JR Vannay C at al | 2001 | Am J Respir Crit Care Med2001，164，5： | 1 |
| 6 | Effects of L-NAME and inhaled nitric oxide on ventilator-induced lung injury in isolated, perfused rabbit lungs 显示文摘 | Broccard AF Feihl F Vannay C | 2004 | Crit Care Med2004，32，9： | 1 |
| 7 | Protective effects ofhy- percapnic acidosis on ventilator-induced lung injury 显示文摘 | Broccard AF Hotchkiss JR Vannay C | 2001 | Am J Respir Crit Care Med2001，164，： | 1 |
| 8 | Effects of L-NAME and inhaled nitric oxide on ventilator- induced lung injury in iso- lated,perfused rabbit lungs 显示文摘 | Broccard AF Feihl F Vannay C | 2004 | Crit Care Med2004，32，9： | 1 |
| 9 | Radiation risk to low fluences of alpha particles may be greater than we thought 显示文摘 | Zhou H Suzuki M Vannais D | 2001 | Proc Natl Acad Sci USA2001，98，14： | 1 |
| 10 | Tectonometamorphic evolution of the Himalayan metamorphic core between the Annapurna and Dhaulagiri, central Nepal显示文摘 | VANNAY J C HODGES K V | 1996 | Metamorphic Geol1996，14，： | 1 |
| 11 | Effects of L-NAME and inhaled nitric oxide on ventilator-induced lung injury in isolated,perfused rabbit lungs显示文摘 | Broccard AF Feihl F Vannay C | 2004 | Crit Care Med2004，32，9： | 1 |
| 12 | Protective effects of hypercapnic acidosis on ventilator-induced lung injury显示文摘 | Broccard A F Hotchkiss J R Vannay C | 2001 | Am J Respir Crit Care Med2001，164，： | 1 |
| 13 | Increased Heat Shock Protein 72 Expression in Celiac Disease显示文摘 | Erna Sziksz Gábor Veres ádám Vannay ágnes Prókai Krisztina Gál Anna ónody Ilma Rita Korponay-Szabó Gy?rgy Reusz András Szabó Tivadar Tulassay András Arató Beáta Szebeni | 2010 | Journal of Pediatric Gastroenterology and Nutrition2010，，5： | 1 |
| 14 | Effects of L-NAME and inhaled nitric oxide on ventilator-induced lung injury in isolated,perfused rabbit lungs显示文摘 | Broccard AF Feihl F Vannay C | 2004 | Critical Care Medicine2004，32，9： | 1 |
| 15 | Protective effects of hypercapnic acidosis on ventilator-induced lung injury 显示文摘 | Broccard AF Hotchkiss JR Vannay C | 2001 | Am J Respir Crit Care Med2001，164，5： | 1 |
| 16 | Association of Genetic Polymorphisms of Vascular Endothelial Growth Factor and Risk for Proliferative Retinopathy of Prematurity显示文摘 | Vannay A Dunai G Banyasz I | 2005 | Pediatr Res2005，57，： | 1 |
| 17 | Protective effects of hypercapnic acidosis on ventilator-induced lung injury显示文摘 | Broccard AF Hotchkiss JR Vannay C | 2001 | Am J Respir Crit Care Med2001，164，5： | 1 |
| 18 | Effects of L-NAME and inhaled nitric oxide on ventilator-induced lung iniury in isolated,perfused rabbit lungs显示文摘 | Broccard A F Feihl F Vannay C | 2004 | Crit Care Med2004，32，9： | 1 |
| 19 | Single-nucleotide poly- morphisms of VEGF gene are associated with risk of congenital val- vuloseptal heart defects 显示文摘 | Vannay A V6s6rhelyi B Kfirnyei M | 2006 | Am Heart J2006，151，4： | 1 |
| 20 | Association of genetic polymorphisms of vascular endothelial growth factor and risk for proliferative retinopathy of prematurity显示文摘 | Vannay A Dunai G Banyasz I Szab6 M Vamos R Treszl k | 2005 | Pediatr Res2005，57，3： | 1 |
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