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| 1 | Treg/Th17 cell balance and phytohaemagglutinin activation of T lymphocytes in peripheral blood of systemic sclerosis patients显示文摘AIM To investigate T-cell activation, the percentage of peripheral T regulatory cells(Tregs), Th17 cells and the circulating cytokine profile in systemic sclerosis(SSc).METHODS We enrolled a total of 24 SSc patients and 16 healthy controls in the study and divided the patients as having diffuse cutaneous SSc(dc SSc, n = 13) or limited cutaneous SSc(lc SSc, n = 11). We performed a further subdivision of the patients regarding the stage of the disease-early, intermediate or late. Peripheral venous blood samples were collected from all subjects. We performed flow cytometric analysis of the activationcapacity of T-lymphocytes upon stimulation with PHA-M and of the percentage of peripheral Tregs and Th17 cells in both patients and healthy controls. We used ELISA to quantitate serum levels of human interleukin(IL)-6, IL-10, tissue growth factor-β1(TGF-β1), and IL-17 A.RESULTS We identified a decreased percentage of CD3+CD69+ cells in PHA-stimulated samples from SSc patients in comparison with healthy controls(13.35% ± 2.90% vs 37.03% ± 2.33%, P < 0.001). However, we did not establish a correlation between the down-regulated CD3+CD69+ cells and the clinical subset, nor regarding the stage of the disease. The activated CD4+CD25+ peripheral lymphocytes were represented in decreased percentage in patients when compared to controls(6.30% ± 0.68% vs 9.36% ± 1.08%, P = 0.016). Regarding the forms of the disease, dc SSc patients demonstrated lower frequency of CD4+CD25+ T cells against healthy subjects(5.95% ± 0.89% vs 9.36% ± 1.08%, P = 0.025). With regard to Th17 cells, our patients demonstrated increased percentage in comparison with controls(18.13% ± 1.55% vs 13.73% ± 1.21%, P = 0.031). We detected up-regulated Th17 cells within the lc SSc subset against controls(20.46% ± 2.41% vs 13.73% ± 1.21%, P = 0.025), nevertheless no difference was found between dc SSc and lc SSc patients. Flow cytometric analysis revealed an increased percentage of CD4+CD25-Foxp3+ in dc SSc patients compared to controls(10.94% ± 1.65% vs 6.88% ± 0.91, P = 0.032). Regarding the peripheral cytokine profile, we detected raised levels of IL-6 [2.10(1.05-4.60) pg/m L vs 0.00 pg/m L, P < 0.001], TGF-β1(19.94 ± 3.35 ng/m L vs 10.03 ± 2.25 ng/m L, P = 0.02), IL-10(2.83 ± 0.44 pg/m L vs 0.68 ± 0.51 pg/m L, P = 0.008), and IL-17 A [6.30(2.50-15.60) pg/m L vs 0(0.00-0.05) pg/m L, P < 0.001] in patients when compared to healthy controls. Furthermore, we found increased circulating IL-10, TGF-β, IL-6 and IL-17 A in the lc SSc subset vs control subjects, as it follows: IL-10(3.32 ± 0.59 pg/m L vs 0.68 ± 0.51 pg/m L, P = 0.003), TGF-β1(22.82 ± 4.99 ng/m L vs 10.03 ± 2.25 ng/m L, P = 0.031), IL-6 [2.08(1.51-4.69) pg/m L vs 0.00 pg/m L, P < 0.001], and IL-17 A [14.50(8.55-41.65) pg/m L vs 0.00(0.00-0.05) pg/m L, P < 0.001]. Furthermore, circulating IL-17 A was higher in lc SSc as opposed to dc SSc subset(31.99 ± 13.29 pg/m L vs 7.14 ± 3.01 pg/m L, P = 0.008). Within the dc SSc subset, raised levels of IL-17 A and IL-6 were detected vs healthy controls: IL-17 A [2.60(0.45-9.80) pg/m L vs 0.00(0.00-0.05) pg/m L, P < 0.001], IL-6 [2.80(1.03-7.23) pg/m L vs 0.00 pg/m L, P < 0.001]. Regarding the stages of the disease, TGF-β1 serum levels were increased in early stage against late stage, independently from the SSc phenotype(30.03 ± 4.59 ng/m L vs 13.08 ± 4.50 ng/m L, P = 0.017).CONCLUSION It is likely that the altered percentage of Th17 and CD4+CD25-Fox P3+ cells along with the peripheral cytokine profile in patients with SSc may play a key role in the pathogenesis of the disease. | Ekaterina Krasimirova Tsvetelina Velikova Ekaterina Ivanova-Todorova Kalina Tumangelova-Yuzeir Desislava Kalinova Vladimira Boyadzhieva Nikolay Stoilov Tsvetelina Yoneva Rasho Rashkov Dobroslav Kyurkchiev | 2017 | World Journal of Experimental Medicine2017，7，3： | 10 |
| 2 | Interleukin-6 compared to the other Th17/Treg related cytokines in inflammatory bowel disease and colorectal cancer显示文摘BACKGROUND The connection between inflammatory bowel disease(IBD)and colorectal cancer(CRC)is well-established,as persistent intestinal inflammation plays a substantial role in both disorders.Cytokines may further influence the inflammation and the carcinogenesis process.AIM To compare cytokine patterns of active IBD patients with early and advanced CRC.METHODS Choosing a panel of cytokines crucial for Th17/Treg differentiation and behavior,in colon specimens,as mRNA biomarkers,and their serum protein levels.RESULTS We found a significant difference between higher gene expression of FoxP3,TGFb1,IL-10,and IL-23,and approximately equal level of IL-6 in CRC patients in comparison with IBD patients.After stratification of CRC patients,we found a significant difference in FoxP3,IL-10,IL-23,and IL-17A mRNA in early cases compared to IBD,and IL-23 alone in advanced CRC.The protein levels of the cytokines were significantly higher in CRC patients compared to IBD patients.CONCLUSION Our findings showed that IL-6 upregulation is essential for both IBD and CRC development until the upregulation of other Th17/Treg related genes(TGFb1,IL-10,IL-23,and transcription factor FoxP3)is a crucial primarily for CRC development.The significantly upregulated IL-6 could be a potential drug target for IBD and prevention of CRC development as well. | Tsvetelina Veselinova Velikova Lyuba Miteva Noyko Stanilov Zoya Spassova Spaska Angelova Stanilova | 2020 | World Journal of Gastroenterology2020，26，16： | 10 |
| 3 | Oxidative stress and some antioxidant systems in acid rain-treated bean plants显示文摘 | V Velikova I Yordanov A Edreva | 2000 | Plant Science2000，，1： | 2 |
| 4 | The clinical value of quality of life assessment in oncology practice-a qualitative study of patient and physician views 显示文摘 | Velikova G Awad N Coles-Gale R | 2008 | Psychooncology2008，17，7： | 1 |
| 5 | Oxidative stress and some antioxidant systems in acid rain-treated bean plants protective role of exogenous polyamines显示文摘 | VELIKOVA V YORDANOV I EDREVA A | 2000 | Plant Sci2000，151，： | 1 |
| 6 | Chemical composition and biological activity of propolis from Turkish and Bulgarian Ori- gion 显示文摘 | Velikova M Bankova V Sorkun K | 2001 | Mellifera2001，，1： | 1 |
| 7 | Factors affecting complications development and mortality after single lung transplant显示文摘Lung transplantation(LT)is a life-saving therapeutic procedure that prolongs survival in patients with end-stage lung disease.Furthermore,as a therapeutic option for high-risk candidates,single LT(SLT)can be feasible because the immediate morbidity and mortality after transplantation are lower compared to sequential single(double)LT(SSLTx).Still,the long-term overall survival is,in general,better for SSLTx.Despite the great success over the years,the early post-SLT period remains a perilous time for these patients.Patients who undergo SLT are predisposed to evolving early or late postoperative complications.This review emphasizes factors leading to post-SLT complications in the early and late periods including primary graft dysfunction and chronic lung allograft dysfunction,native lung complications,anastomosis complications,infections,cardiovascular,gastrointestinal,renal,and metabolite complications,and their association with morbidity and mortality in these patients.Furthermore,we discuss the incidence of malignancy after SLT and their correlation with immunosuppression therapy. | Metodija Sekulovski Bilyana Simonska Milena Peruhova Boris Krastev Monika Peshevska-Sekulovska Lubomir Spassov Tsvetelina Velikova | 2021 | World Journal of Transplantation2021，11，8： | 1 |
| 8 | Plant responses to drought, acclimation, and stress tolerance显示文摘 | Yordanov I Velikova V Tsonev T | 2000 | Photosynthetic2000，38，： | 1 |
| 9 | Oxidative stress and some autioxidant systems in acid rain-treated bean plants:protective role of exogenous polyamines显示文摘 | Velikova V Yordanov I Edreva A | 2000 | Plant Science2000，151，： | 1 |
| 10 | The European Organization for Research and Treatment of Cancer Quality of Life Questionnaire for patients with Bone Metastases: The EORTC QLQ-BM22显示文摘 | Chow E Hird A Velikova G | 2009 | Eur J Cancer2009，45，7： | 1 |
| 11 | Learning Bayesian networks for clinical time series analysis 显示文摘 | van der Heijden M Velikova M Lucas PJ | 2013 | J Biomed Inform2013，464，13： | 1 |
| 12 | On the interplay of machine learning and background knowledge in image in- terpretation by Bayesian networks显示文摘 | Velikova M Lucas P J F Samulski M | 2013 | Artificial Intelligence in Medicine2013，57，1： | 1 |
| 13 | Plant Responses to Drought, Acclimation, and Stress Tolerance显示文摘 | I. Yordanov V. Velikova T. Tsonev | 2000 | Photosynthetica2000，，2： | 1 |
| 14 | Oxidative stress and some antioxidant systems in acid rain-treated bean plants protective role of exogenous polyamines显示文摘 | Velikova V Yordanov I Edreva A | 2000 | Plant Sci2000，151，： | 1 |
| 15 | Factor analysis kf anx- iety and depression among scale from a large cancer poprlation 显示文摘 | Smith AB Selby P J Velikova C | 2002 | Psychol Psychother2002，75，： | 1 |
| 16 | Standard chemotherapy with or without bevacizumab in advanced ovarian cancer: quality-of-life outcomes from the International Collaboration on Ovarian Neoplasms (ICON7) phase 3 randomised trial显示文摘 | Dan Stark Matthew Nankivell Eric Pujade-Lauraine Gunnar Kristensen Lorraine Elit Martin Stockler Felix Hilpert Andrés Cervantes Julia Brown Anne Lanceley Galina Velikova Eduardo Sabate Jacobus Pfisterer Mark S Carey Philip Beale Wendi Qian Ann Marie Swart | 2013 | Lancet Oncology2013，，： | 1 |
| 17 | The European Organization forResearch and Treatment of Cancer Quality of Life Questionnairefor patients with bone metastases:The EORTC QLQ - BM22显示文摘 | Chow E Hird A Velikova G etal | 2009 | Eur J Cancer2009，457，： | 1 |
| 18 | Oxidative stress and some antioxidant systems in acid rain-treated bean plants: Protective role of exogenous polyamines 显示文摘 | Velikova V Yordanov I Edreva A | 2000 | Plant Science2000，151，1： | 1 |
| 19 | Oxidative stress and some antioxidant systems in acid rain-treated bean plants protective role of exogenous polyamines 显示文摘 | Velikova V Yordanov I Edreva A | 2000 | Plant Sci2000，151，2： | 1 |
| 20 | Oxidative stress and some antioxidant systems in acid rain-treated beanplants:protective role of exogenous polyamines显示文摘 | Velikova V Yordanov I Edreva A | 2000 | Plant Science2000，151，1： | 1 |
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