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| 1 | The identities and anti-herpes simplex virus activity of Clinacanthus nutans and Clinacanthus siamensis显示文摘Objective:To distinguish the difference among the Clinacanthus nutans(Burm.f.)Lindau(C.nutans)and Clinacanthus siamensis Bremek(C.siamensis)by assessing pharmacognosy characteristics,molecular aspect and also to evaluate their anti-herpes simplex virus(HSV)type 1 and type 2 activities.Methods:Macroscopic and microscopic evaluation were performed according to WHO Geneva guideline.Stomatal number,stomatal index and palisade ratio of leaves were evaluated.Genomic DNA was extracted by modified CTAB method and ITS region was amplified using PGR and then sequenced.Dry leaves were subsequently extracted with n-hexane,dichloromethane and methanol and antiviral activity was performed using plaque reduction assay and the cytotoxicity of the extracts on Vero cells was determined by MTT assay.Results:Cross section of midrib and stem showed similar major components.Leaf measurement index of stomatal number,stomatal index and palisade ratio of C.nutans were 168.32±29.49,13.83±0.86 and 6.84±0.66,respectively,while C.siamensis were 161.60±18.04,11.93±0.81and 3.37±0.31,respectively.The PCR amplification of ITS region generated the PGR product approximately 700 bp in size.There were 34 polymorphisms within the ITS region which consisted of 11 Indels and 23 nucleotide substitutions.The IC_(50)values of C.nutans extracted with n-hexane,dichloromethane and methanol against HSV-1 were(32.05±3.63)μg/mL,(44.50±2.66)μg/mL,(64.93±7.00)μg/mL,respectively where as those of C.siamensis were(60.00±11.61)μg/mL,(55.69+4.41)μg/mL,(37.39±5.85)μg/mL,respectively.Anti HSV-2 activity of n-hexane,dichloromethane and methanol C.nutans leaves extracts were(72.62±12.60)μg/mL,(65.19±21.45)μg/mL,(65.13±2.22)μg/mL,respectively where as those of C.siamensis were(46.52±4.08)μg/mL,(49.63±2.59)μg/mL,(72.64±6.52)μg/mL,respectively.Conclusions:The combination of macroscopic,microscopic and biomolecular method are able to authenticate these closely related plants and both of them have a potency to be an anti-HSV agent. | Paween Kunsorn Nijsiri Ruangrungsi Vimolmas Lipipun Ariya Khanboon Kanchana Rungsihirunrat | 2013 | Asian Pacific Journal of Tropical Biomedicine2013，3，4： | 6 |
| 2 | Development of polyurethane foam dressing containing silver and asiaticoside for healing of dermal wound显示文摘Polyurethane foam dressings for dermal wounds were formulated with natural polyols in order to improve the foam characteristics and the release of 2 active agents,silver and asiaticoside(AS)as an antimicrobial agent and an herbal wound healing agent,respectively.The foam was instantly formed by interaction of polyols and diisocyanate.Hydroxypropyl methylcellulose,chitosan and sodium alginate were individually mixed with themain polyols,polypropylene glycol,in the formulation while the active componentswere impregnated into the obtained foam dressing sheets.Although the type and amount of the natural polyols slightly affected the pore size,water sorption-desorption profile and compression strength of the obtained foam sheets,a prominent effect was found in the release of both active components.Among natural polyols formulations,foam sheets with alginate showed the highest silver and AS release.Non-cytotoxicity of these foam sheets to human fibroblast cells was confirmed.Antimicrobial testing on four bacteria strains showed that 1mg/cm^2 silver in formulations with 6%of natural polyols and without natural polyols had sufficient content of the silver release with comparable inhibition zone and significantly larger zone than other formulations.In pig study,the foam dressing with 6%alginate,1mg/cm^2 silver and 5%AS could improve wound healing in both the percentage of the wound closure and histological parameters of the dermal wound without any dermatologic reactions.In conclusion,this innovative foam dressing had potential to be a good candidate for wound treatment. | Nantaporn Namviriyachote Vimolmas Lipipun Yada Akkhawattanangkul Phingphol Charoonrut Garnpimol C.Ritthidej | 2019 | Asian Journal of Pharmaceutical Sciences2019，14，1： | 6 |
| 3 | Synthesis and anti-herpes simplex viral activity of monoglycosyl diglycerides显示文摘 | Wicharn Janwitayanuchit Khanit Suwanborirux Chamnan Patarapanich Sunibhond Pummangura Vimolmas Lipipun Tirayut Vilaivan | 2003 | Phytochemistry2003，，7： | 2 |
| 4 | Efficacy of Thai medicinal plant extracts against herpes simplex virus type 1 infection in vitro and in vivo显示文摘 | Vimolmas Lipipun Masahiko Kurokawa Rutt Suttisri Pagorn Taweechotipatr Pornpen Pramyothin Masao Hattori Kimiyasu Shiraki | 2003 | Antiviral Research2003，，3： | 1 |
| 5 | Efficacy of Thai medicinal plant extracts against herpes simplex virus type 1 infection in vitro and in vivo显示文摘 | VIMOLMAS L MASAHIKO K RUTT S | 2003 | Antiviral Res2003，60，3： | 1 |
| 6 | Inhibitory activity of oxyresveratrol on wild-type and drug-resistant varicella-zoster virus replication in vitro显示文摘 | Pattaraporn Sasivimolphan Vimolmas Lipipun Kittisak Likhitwitayawuid Masaya Takemoto Pornpen Pramyothin Masao Hattori Kimiyasu Shiraki | 2009 | Antiviral Research2009，，1： | 1 |
| 7 | Anti-herpes simplex virus (HSV-1) activity of oxyresveratrol derived from Thai medicinal plant: Mechanism of action and therapeutic efficacy on cutaneous HSV-1 infection in mice显示文摘 | Taksina Chuanasa Jurairatana Phromjai Vimolmas Lipipun Kittisak Likhitwitayawuid Mikiko Suzuki Pornpen Pramyothin Masao Hattori Kimiyasu Shiraki | 2008 | Antiviral Research2008，，1： | 1 |
| 8 | Phenolics with antiviral activity from Millettia Erythrocalyx and Artocarpus Lakoocha显示文摘 | Kittisak Likhitwitayawuid Boonchoo Sritularak Kanokwan Benchanak Vimolmas Lipipun Judy Mathew Raymond F. Schinazi | 2005 | Natural Product Research2005，，2： | 1 |
| 9 | Studies on the Chemical Constituents of Stem Bark of Millettia leucantha:Isolation of New Chalcones with Cytotoxie,Anti-herpes Simplex Virus and Anti-inflammatory Activities显示文摘 | Vimolmas L Nijsiri R | 2003 | Chem Pharm BuU2003，51，2： | 1 |
| 10 | Efficacy of Thai medicinal plant extracts against herpes simplex virus type 1 infection in vitro and in vivo显示文摘 | Vimolmas L Masahiko K Rutt S | 2003 | Antiviral Research2003，，60： | 1 |
| 11 | Efficacy of Thai medicinal plant extracts against herpes simplex virus type 1 infection in vitro and in vivo 显示文摘 | Vimolmas Lipipun Masahiko Kurokawa Rutt Suttisri | 2003 | Antiviral Research2003，60，3： | 1 |
| 12 | Anti-herpes simplex virus (HSV-1) activity of oxyresveratrol derived from Thai medicinal plant: Mechanism of action and therapeutic efficacy on cutaneous HSV-1 infection in mice显示文摘 | Taksina Chuanasa Jurairatana Phromjai Vimolmas Lipipun Kittisak Likhitwitayawuid Mikiko Suzuki Pornpen Pramyothin Masao Hattori Kimiyasu Shiraki | 2008 | Antiviral Research2008，，1： | 1 |
| 13 | Effect of cationic microemulsion on hemolysis and immune cell uptake显示文摘Squalene-based oil-in-water nanoemulsions, constituents in influenza vaccines, have been approved in Europe as human licensed adjuvants (1)With similar constituents but easier manufacturing procedure and greater physical stability [2],microemulsion was of interest. To improve cellular uptake,positive surface charge of nanosystems has been reported(3)In the present study, cationic microemulsions were prepared, characterized and evaluated in immune cell. | Sakalanunt Lamaisakul Angkana Tantituvanont Vimolmas Lipipun Garnpimol C.Ritthidej | 2016 | Asian Journal of Pharmaceutical Sciences2016，11，1： | 0 |
| 14 | Development of novel cationic microemulsion as parenteral adjuvant for influenza vaccine显示文摘Squalene-based oil-in-water(O/W)emulsions have been used as effective and safe adjuvants in approved influenza vaccines.However,there are concerns regarding the safety and side effects of increasing risk of narcolepsy.In present study,novel O/W microemulsions(MEs)containing wheat germ oil,D-alpha tocopheryl polyethylene glycol 1000 succinate(TPGS)and Cremophor EL(CreEL)or Solutol HS15 were formulated with/without a cationic surfactant,cetyltrimethylammonium bromide(CTAB)and then sterilized by autoclaving.Their physical properties and biological efficacies were evaluated.The results demonstrated that autoclaving reduced the droplet size to^20 nm with narrow size distributions resulting in monodisperse systems with good stability up to 3 years.Hemolytic activity,viscosity,pH,and osmolality were appropriate for parenteral use.Bovine serum albumin(BSA),a model antigen,after mixing with MEs retained the protein integrity,assessed by SDS-PAGE and CD spectroscopy.Greater percentages of 28 SC cell viability were observed from CreEL-based MEs.Uptake of FITC-BSA-MEs increased with the increasing concentration of CTAB confirmed by CLSM images.Furthermore,cationic CreEL-based MEs could induce Th1 cytokine synthesis with an increase in TNF-α and IL-12 levels and a decrease in IL-10 level.In vivo immunization study in mice of adjuvants admixed with influenza virus solution revealed that nonionic and selected cationic CreEL-MEs enhanced immune responses as measured by influenza-specific serum antibody titers and hemagglutination inhibition titers.Particularly,cationic CreEL-based ME showed better humoral and cellular immunity with higher IgG2 a titer than nonionic CreEL-based ME and antigen alone.No differences in immune responses were observed between mice immunized with selected cationic CreEL-based ME and marketed adjuvant.In addition,the selected ME induced antigen-sparing while retained immune stimulating effects compared to antigen alone.No inflammatory change in muscle fiber structure was observed.Accordingly,the developed cationic CreEL-based ME had potential as novel adjuvant for parenteral influenza vaccine. | Sakalanunt Lamaisakul Angkana Tantituvanont Vimolmas Lipipun Garnpimol Ritthidej | 2020 | Asian Journal of Pharmaceutical Sciences2020，15，5： | 0 |
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