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| 1 | Atrophic gastritis and gastric cancer tissue miRNome analysis reveals hsa-miR-129-1 and hsa-miR-196a as potential early diagnostic biomarkers显示文摘BACKGROUND Gastric cancer(GC)is one of the most frequently diagnosed tumor globally.In most cases,GC develops in a stepwise manner from chronic gastritis or atrophic gastritis(AG)to cancer.One of the major issues in clinical settings of GC is diagnosis at advanced disease stages resulting in poor prognosis.Micro RNAs(mi RNAs)are small noncoding molecules that play an essential role in a variety of fundamental biological processes.However,clinical potential of mi RNA profiling in the gastric cancerogenesis,especially in premalignant GC cases,remains unclear.AIM To evaluate the AG and GC tissue mi RNomes and identify specific mi RNAs’potential for clinical applications(e.g.,non-invasive diagnostics).METHODS Study included a total of 125 subjects:Controls(CON),AG,and GC patients.All study subjects were recruited at the Departments of Surgery or Gastroenterology,Hospital of Lithuanian University of Health Sciences and divided into the profiling(n=60)and validation(n=65)cohorts.Total RNA isolated from tissue samples was used for preparation of small RNA sequencing libraries and profiled using next-generation sequencing(NGS).Based on NGS data,deregulated mi RNAs hsa-mi R-129-1-3 p and hsa-mi R-196 a-5 p were analyzed in plasma samples of independent cohort consisting of CON,AG,and GC patients.Expression level of hsa-mi R-129-1-3 p and hsa-mi R-196 a-5 p was determined using the quantitative real-time polymerase chain reaction and 2-ΔΔCt method.RESULTS Results of tissue analysis revealed 20 differentially expressed mi RNAs in AG group compared to CON group,129 deregulated mi RNAs in GC compared to CON,and 99 altered mi RNAs comparing GC and AG groups.Only 2 mi RNAs(hsa-mi R-129-1-3 p and hsa-mi R-196 a-5 p)were identified to be step-wise deregulated in healthy-premalignant-malignant sequence.Area under the curve(AUC)-receiver operating characteristic analysis revealed that expression level of hsa-mi R-196 a-5 p is significant for discrimination of CON vs AG,CON vs GC and AG vs GC and resulted in AUCs:88.0%,93.1%and 66.3%,respectively.Comparing results in tissue and plasma samples,hsa-mi R-129-1-3 p was significantly down-regulated in GC compared to AG(P=0.0021 and P=0.024,tissue and plasma,respectively).Moreover,analysis revealed that hsa-mi R-215-3 p/5 p and hsa-mi R-934 were significantly deregulated in GC based on Helicobacter pylori(H.pylori)infection status[log2 fold change(FC)=-4.52,P-adjusted=0.02;log2 FC=-4.00,P-adjusted=0.02;log2 FC=6.09,P-adjusted=0.02,respectively].CONCLUSION Comprehensive mi RNome study provides evidence for gradual deregulation of hsa-mi R-196 a-5 p and hsa-mi R-129-1-3 p in gastric carcinogenesis and found hsami R-215-3 p/5 p and hsa-mi R-934 to be significantly deregulated in H.pylori carrying GC patients. | Greta Varkalaite Evelina Vaitkeviciute Ruta Inciuraite Violeta Salteniene Simonas Juzenas Vytenis Petkevicius Rita Gudaityte Antanas Mickevicius Alexander Link Limas Kupcinskas Marcis Leja Juozas Kupcinskas Jurgita Skieceviciene | 2022 | World Journal of Gastroenterology2022，28，6： | 2 |
| 2 | Polymorphisms of micro RNA target genes IL12B, INSR, CCND1 and IL10 in gastric cancer显示文摘AIM To evaluate associations between mi RNA target genes IL12B,INSR,CCND1 and IL10 polymorphisms and gastric cancer(GC)in European population.METHODS Gene polymorphisms were analyzed in 508 controls and474 GC patients from 3 tertiary centers in Germany,Lithuania and Latvia.Controls were patients from the out-patient departments,who were referred for upper endoscopy because of dyspeptic symptoms and had no history of previous malignancy.Gastric cancer(GC)patients had histopathological verification of gastric adenocarcinoma.Genomic DNA was extracted using salting out method from peripheral blood mononuclear cells.IL12B T>G(rs1368439),INSR T>C(rs1051690),CCND1 A>C(rs7177)and IL10 T>C(rs3024498)SNPs were genotyped by the real-time polymerase chain reaction.Associations between gene polymorphism and GC were evaluated using multiple logistic regression analysis with adjustment for sex,age and country of birth.RESULTS We observed similar distribution of genotypes and allelic frequencies of all polymorphisms between GC patients and controls except of INSR rs1051690.The frequency of the T allele of INSR gene was significantly higher in GC patients than in controls(23.26%and 19.19%respectively,P=0.028).CT genotype was also more prevalent in patients compared to control group(38.48%and 30.12%respectively,P<0.021).Logistic regression analysis revealed that only one polymorphism(rs1051690 in INSR gene)was associated with increased risk of GC.Carriers of CT genotype had higher odds of GC when compared to CC genotype(OR=1.45,95%PI:1.08-1.95,P=0.01).Similar association was observed in a dominant model for INSR gene,where comparison of TT+CT vs CC genotypes showed an increased risk of GC(OR=1.44,95%PI:1.08-1.90,P=0.01).Other analyzed SNPs were not associated with the presence of GC.CONCLUSION INSR rs1051690 SNP is associated with increased risk of GC,while polymorphisms in IL12B,CCND1 and IL10genes are not linked with the presence of GC. | Vytenis Petkevicius Violeta Salteniene Simonas Juzenas Thomas Wex Alexander Link Marcis Leja Ruta Steponaitiene Jurgita Skieceviciene Limas Kupcinskas Laimas Jonaitis Gediminas Kiudelis Peter Malfertheiner Juozas Kupcinskas | 2017 | World Journal of Gastroenterology2017，23，19： | 2 |
| 3 | Investigation of volatile organic compounds(VOCs) emission beyond the territory of oil terminals during different seasons显示文摘 | TATJANA P VYTENIS Z PETRAS V | 2011 | Journal of Environmental Engineering and Landscape Management2011，19，1： | 1 |
| 4 | Fires due to electric arcing: Can 'Cause' beads be distinguished from 'Victim' beads by physical or chemical testing显示文摘 | Vytenis Babrauskas | 2002 | Fire Science and Technology2002，，10： | 1 |
| 5 | Estimating Large Pool Fire Burning Rates 显示文摘 | Vytenis Babrauskas | 1983 | Fire Technology1983，19，4： | 1 |
| 6 | Effective measurement techniques for heat,smoke, and toxic fire gases显示文摘 | Vytenis Babrauskas | 1991 | Fire Safety1991，17，1： | 1 |
| 7 | Free Burning Fires 显示文摘 | Vytenis Babrauskas | 1986 | Fire Safety Journal1986，11，12： | 1 |
| 8 | Regulation of connexin36 gap junction channels by n ‐alkanols and arachidonic acid显示文摘 | Alina Marandykina Nicolás Palacios‐Prado Lina Rimkut? Vytenis A. Skeberdis Feliksas F. Bukauskas | 2013 | The Journal of Physiology2013，，8： | 1 |
| 9 | The cone calorimeter used for predictions of the full-scale burning behavior of upholstered furniture显示文摘 | Vytenis Babrauskas Djebar Baroudi Jukka Myllym ki | 1997 | Fire and Materials1997，，21： | 1 |
| 10 | pH‐dependent modulation of connexin‐based gap junctional uncouplers显示文摘 | Vytenis A.Skeberdis LinaRimkute AisteSkeberdyte NerijusPaulauskas Feliksas F.Bukauskas | 2011 | The Journal of Physiology2011，，14： | 1 |
| 11 | Structure and function of b3-adrenergic receptors显示文摘 | Vytenis Arvydas Skeberdis | 2004 | Medicine Kaunas2004，40，5： | 1 |
| 12 | Structure and function of beta 3-adrenergic receptors显示文摘 | Vytenis AS | 2004 | Medicina (Kaunas)2004，40，5： | 1 |
| 13 | rapid HPLC determation of total homocysteine and other thiols in serum and plasma:sec differences and correlation with cobalamin and folate consentrations in healthy subjects显示文摘 | Donade W Vytenis J | 1994 | Clind Chem1994，40，6： | 1 |
| 14 | Fires due to Electric Arcing: Can 'Cause' Beads Be Distinguished from ' Victim' Beads by Physical or Chemical Testing显示文摘 | Vytenis Babrauskas | 2002 | Fire Science and Technology2002，，10： | 1 |
| 15 | Developingrational, perform- ance-based fire safety requirements in model building codes显示文摘 | Richard W Vytenis B | 1994 | Fire and Materials1994，18，： | 1 |
| 16 | Estimating large pool fire burning rates 显示文摘 | Babrauskas Vytenis | 2003 | Fire Technol2003，139，4： | 1 |
| 17 | Prevalence of C282Y, H63D, and S65C mutations in hereditary HFE-hemochromatosis gene in Lithuanian population显示文摘 | Laimutis Kucinskas Simonas Juzenas Jurgita Sventoraityte Ruta Cedaviciute Astra Vitkauskiene Vytenis Kalibatas Jurate Kondrackiene Limas Kupcinskas | 2012 | Annals of Hematology2012，，4： | 1 |
| 18 | Heat release rate: The single most important variable in fire hazard显示文摘 | Babrauskas Vytenis Peacock Richard D | 1992 | Fire Safety J1992，18，： | 1 |
| 19 | Conditions for smoke toxicity measurement显示文摘 | A Leigh Griffith P Richard G Gann Vytenis Babrauskas etal | 1994 | Fire andMaterials1994，3，19： | 1 |
| 20 | The cone calorimeter used for predictions of the full-scale burning behavior of upholstered furniture 显示文摘 | Vytenis Babrauskas Djebar Baroudi Jukka Myllym ki | 1997 | Fireand Materials1997，，21： | 1 |
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